ID   A0A672I2V8_SALFA        Unreviewed;       308 AA.
AC   A0A672I2V8;
DT   17-JUN-2020, integrated into UniProtKB/TrEMBL.
DT   17-JUN-2020, sequence version 1.
DT   12-AUG-2020, entry version 2.
DE   RecName: Full=NADH-cytochrome b5 reductase {ECO:0000256|RuleBase:RU361226};
DE            EC=1.6.2.2 {ECO:0000256|RuleBase:RU361226};
GN   Name=LOC115391864 {ECO:0000313|Ensembl:ENSSFAP00005035928};
OS   Salarias fasciatus (Jewelled blenny) (Blennius fasciatus).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Neoteleostei; Acanthomorphata;
OC   Ovalentaria; Blenniimorphae; Blenniiformes; Blennioidei; Blenniidae;
OC   Salariinae; Salarias.
OX   NCBI_TaxID=181472 {ECO:0000313|Ensembl:ENSSFAP00005035928};
RN   [1] {ECO:0000313|Ensembl:ENSSFAP00005035928}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RG   Wellcome Sanger Institute Data Sharing;
RL   Submitted (JUN-2019) to the EMBL/GenBank/DDBJ databases.
RN   [2] {ECO:0000313|Ensembl:ENSSFAP00005035928}
RP   IDENTIFICATION.
RG   Ensembl;
RL   Submitted (MAR-2020) to UniProtKB.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2 [Fe(III)-cytochrome b5] + NADH = 2 [Fe(II)-cytochrome b5] +
CC         H(+) + NAD(+); Xref=Rhea:RHEA:46680, Rhea:RHEA-COMP:10438, Rhea:RHEA-
CC         COMP:10439, ChEBI:CHEBI:15378, ChEBI:CHEBI:29033, ChEBI:CHEBI:29034,
CC         ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.6.2.2;
CC         Evidence={ECO:0000256|ARBA:ARBA00001310,
CC         ECO:0000256|RuleBase:RU361226};
CC   -!- COFACTOR:
CC       Name=FAD; Xref=ChEBI:CHEBI:57692;
CC         Evidence={ECO:0000256|ARBA:ARBA00001974,
CC         ECO:0000256|RuleBase:RU361226};
CC   -!- SIMILARITY: Belongs to the flavoprotein pyridine nucleotide cytochrome
CC       reductase family. {ECO:0000256|ARBA:ARBA00006105,
CC       ECO:0000256|RuleBase:RU361226}.
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DR   Ensembl; ENSSFAT00005037280; ENSSFAP00005035928; ENSSFAG00005018054.
DR   Proteomes; UP000472267; Chromosome: 9.
DR   Gene3D; 3.40.50.80; -; 1.
DR   InterPro; IPR001834; CBR-like.
DR   InterPro; IPR008333; Cbr1-like_FAD-bd_dom.
DR   InterPro; IPR017927; FAD-bd_FR_type.
DR   InterPro; IPR001709; Flavoprot_Pyr_Nucl_cyt_Rdtase.
DR   InterPro; IPR039261; FNR_nucleotide-bd.
DR   InterPro; IPR001433; OxRdtase_FAD/NAD-bd.
DR   InterPro; IPR017938; Riboflavin_synthase-like_b-brl.
DR   Pfam; PF00970; FAD_binding_6; 1.
DR   Pfam; PF00175; NAD_binding_1; 1.
DR   PRINTS; PR00406; CYTB5RDTASE.
DR   PRINTS; PR00371; FPNCR.
DR   SUPFAM; SSF52343; SSF52343; 1.
DR   SUPFAM; SSF63380; SSF63380; 1.
DR   PROSITE; PS51384; FAD_FR; 1.
PE   3: Inferred from homology;
KW   FAD {ECO:0000256|ARBA:ARBA00022827, ECO:0000256|RuleBase:RU361226};
KW   Flavoprotein {ECO:0000256|ARBA:ARBA00022630,
KW   ECO:0000256|RuleBase:RU361226}; Membrane {ECO:0000256|SAM:Phobius};
KW   NAD {ECO:0000256|RuleBase:RU361226};
KW   Oxidoreductase {ECO:0000256|RuleBase:RU361226};
KW   Transmembrane {ECO:0000256|SAM:Phobius};
KW   Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT   TRANSMEM        12..31
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   DOMAIN          47..159
FT                   /note="FAD-binding FR-type"
FT                   /evidence="ECO:0000259|PROSITE:PS51384"
SQ   SEQUENCE   308 AA;  34504 MW;  57E51046B30AD88E CRC64;
     RRASRSAAVL QMLPLLAAVS VVLLSVLLVF LRTFSGEKRK RTLLDPNVKY PLPLIHKQEI
     SHDTKKLRFG LPSGEHALGL PVGQHVYLSA RVGGSLVVRA YTPISSDQDL GYVDLLVKVY
     HRNTHPSFPE GGKLSQHLDS LSIGDTVDFR GPSGLLVYKD HGRFSIRPDK KAEPQVRRFR
     EVAMIAGGTG ITPMLQLIRR ITAEPSDRTK CSLIFANQTE KDILLREELE QLETEHPENL
     RVWFTLDRPE PGWRFGSGFV TRDMMEQRLP AAAPDVLVVL CGPPAMIQNA CLPNLDRLGH
     QPHNIFTY
//