ID A0A650CH07_SULOH Unreviewed; 445 AA. AC A0A650CH07; DT 22-APR-2020, integrated into UniProtKB/TrEMBL. DT 22-APR-2020, sequence version 1. DT 28-JUN-2023, entry version 12. DE RecName: Full=Signal recognition particle 54 kDa protein {ECO:0000256|HAMAP-Rule:MF_00306}; DE Short=SRP54 {ECO:0000256|HAMAP-Rule:MF_00306}; GN Name=srp54 {ECO:0000256|HAMAP-Rule:MF_00306}; GN ORFNames=D1869_07510 {ECO:0000313|EMBL:QGR17046.1}, HNQ62_000226 GN {ECO:0000313|EMBL:MBB5252508.1}; OS Sulfurisphaera ohwakuensis. OC Archaea; Thermoproteota; Thermoprotei; Sulfolobales; Sulfolobaceae; OC Sulfurisphaera. OX NCBI_TaxID=69656 {ECO:0000313|EMBL:QGR17046.1, ECO:0000313|Proteomes:UP000427373}; RN [1] {ECO:0000313|EMBL:QGR17046.1, ECO:0000313|Proteomes:UP000427373} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=TA-1 {ECO:0000313|EMBL:QGR17046.1, RC ECO:0000313|Proteomes:UP000427373}; RA Counts J.A., Kelly R.M.; RT "Genome Sequences from Six Type Strain Members of the Archaeal Family RT Sulfolobaceae: Acidianus ambivalens, Acidianus infernus, Metallosphaera RT prunae, Stygiolobus azoricus, Sulfolobus metallicus, and Sulfurisphaera RT ohwakuensis."; RL Submitted (OCT-2019) to the EMBL/GenBank/DDBJ databases. RN [2] {ECO:0000313|EMBL:MBB5252508.1, ECO:0000313|Proteomes:UP000582213} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=DSM 12421 {ECO:0000313|EMBL:MBB5252508.1, RC ECO:0000313|Proteomes:UP000582213}; RA Goeker M.; RT "Genomic Encyclopedia of Type Strains, Phase IV (KMG-IV): sequencing the RT most valuable type-strain genomes for metagenomic binning, comparative RT biology and taxonomic classification."; RL Submitted (AUG-2020) to the EMBL/GenBank/DDBJ databases. CC -!- FUNCTION: Involved in targeting and insertion of nascent membrane CC proteins into the cytoplasmic membrane. Binds to the hydrophobic signal CC sequence of the ribosome-nascent chain (RNC) as it emerges from the CC ribosomes. The SRP-RNC complex is then targeted to the cytoplasmic CC membrane where it interacts with the SRP receptor FtsY. CC {ECO:0000256|HAMAP-Rule:MF_00306}. CC -!- SUBUNIT: Part of the signal recognition particle protein translocation CC system, which is composed of SRP and FtsY. Archaeal SRP consists of a CC 7S RNA molecule of 300 nucleotides and two protein subunits: SRP54 and CC SRP19. {ECO:0000256|HAMAP-Rule:MF_00306}. CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00306}. CC Note=The SRP-RNC complex is targeted to the cytoplasmic membrane. CC {ECO:0000256|HAMAP-Rule:MF_00306}. CC -!- DOMAIN: Composed of three domains: the N-terminal N domain, which is CC responsible for interactions with the ribosome, the central G domain, CC which binds GTP, and the C-terminal M domain, which binds the RNA and CC the signal sequence of the RNC. {ECO:0000256|HAMAP-Rule:MF_00306}. CC -!- SIMILARITY: Belongs to the GTP-binding SRP family. SRP54 subfamily. CC {ECO:0000256|ARBA:ARBA00005450, ECO:0000256|HAMAP-Rule:MF_00306}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; JACHFY010000001; MBB5252508.1; -; Genomic_DNA. DR EMBL; CP045484; QGR17046.1; -; Genomic_DNA. DR AlphaFoldDB; A0A650CH07; -. DR KEGG; soh:D1869_07510; -. DR OrthoDB; 52849at2157; -. DR Proteomes; UP000427373; Chromosome. DR Proteomes; UP000582213; Unassembled WGS sequence. DR GO; GO:0048500; C:signal recognition particle; IEA:UniProtKB-UniRule. DR GO; GO:0008312; F:7S RNA binding; IEA:UniProtKB-UniRule. DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-UniRule. DR GO; GO:0003924; F:GTPase activity; IEA:UniProtKB-UniRule. DR GO; GO:0006614; P:SRP-dependent cotranslational protein targeting to membrane; IEA:InterPro. DR CDD; cd17875; SRP54_G; 1. DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1. DR Gene3D; 1.20.120.140; Signal recognition particle SRP54, nucleotide-binding domain; 1. DR Gene3D; 1.10.260.30; Signal recognition particle, SRP54 subunit, M-domain; 1. DR HAMAP; MF_00306; SRP54; 1. DR InterPro; IPR003593; AAA+_ATPase. DR InterPro; IPR027417; P-loop_NTPase. DR InterPro; IPR036891; Signal_recog_part_SRP54_M_sf. DR InterPro; IPR013822; Signal_recog_particl_SRP54_hlx. DR InterPro; IPR004125; Signal_recog_particle_SRP54_M. DR InterPro; IPR036225; SRP/SRP_N. DR InterPro; IPR022941; SRP54. DR InterPro; IPR000897; SRP54_GTPase_dom. DR InterPro; IPR042101; SRP54_N_sf. DR PANTHER; PTHR11564:SF5; SIGNAL RECOGNITION PARTICLE 54 KDA PROTEIN; 1. DR PANTHER; PTHR11564; SIGNAL RECOGNITION PARTICLE 54K PROTEIN SRP54; 1. DR Pfam; PF00448; SRP54; 1. DR Pfam; PF02881; SRP54_N; 1. DR Pfam; PF02978; SRP_SPB; 1. DR SMART; SM00382; AAA; 1. DR SMART; SM00962; SRP54; 1. DR SMART; SM00963; SRP54_N; 1. DR SUPFAM; SSF47364; Domain of the SRP/SRP receptor G-proteins; 1. DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1. DR SUPFAM; SSF47446; Signal peptide-binding domain; 1. PE 3: Inferred from homology; KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490, ECO:0000256|HAMAP-Rule:MF_00306}; KW GTP-binding {ECO:0000256|ARBA:ARBA00023134, ECO:0000256|HAMAP- KW Rule:MF_00306}; KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|HAMAP- KW Rule:MF_00306}; KW Ribonucleoprotein {ECO:0000256|ARBA:ARBA00023274, ECO:0000256|HAMAP- KW Rule:MF_00306}; KW RNA-binding {ECO:0000256|ARBA:ARBA00022884, ECO:0000256|HAMAP- KW Rule:MF_00306}; KW Signal recognition particle {ECO:0000256|ARBA:ARBA00023135, KW ECO:0000256|HAMAP-Rule:MF_00306}. FT DOMAIN 1..81 FT /note="Signal recognition particle SRP54 helical bundle" FT /evidence="ECO:0000259|SMART:SM00963" FT DOMAIN 94..297 FT /note="AAA+ ATPase" FT /evidence="ECO:0000259|SMART:SM00382" FT DOMAIN 95..292 FT /note="SRP54-type proteins GTP-binding" FT /evidence="ECO:0000259|SMART:SM00962" FT BINDING 102..109 FT /ligand="GTP" FT /ligand_id="ChEBI:CHEBI:37565" FT /evidence="ECO:0000256|HAMAP-Rule:MF_00306" FT BINDING 184..188 FT /ligand="GTP" FT /ligand_id="ChEBI:CHEBI:37565" FT /evidence="ECO:0000256|HAMAP-Rule:MF_00306" FT BINDING 244..247 FT /ligand="GTP" FT /ligand_id="ChEBI:CHEBI:37565" FT /evidence="ECO:0000256|HAMAP-Rule:MF_00306" SQ SEQUENCE 445 AA; 49816 MW; B607105D7AD86454 CRC64; MLDNLKDAVR KFLGSSNYDK AVNDFIKELQ ISLIKSDVNV KLVSNLTQKI KDRLEKEKPP TAIERREWFI SIVYDELSKL FGGDINPEIT PKKIPYVIML VGVQGSGKTT TAGKLALFYK KKGYKVGLVA ADVYRPAAYD QLVQIGKQIN VPVYGEPNNT DAVGIAKRGV EKFLSEKYDI IIVDTAGRHG YGEEVKLLEE MKNMYSEIKP DEVILVIDAS IGQKAYDLAS RFHQASPIGS IIVTKMDGTA KGGGALSAVA ATGAVIKFIG TGEKLDELEV FNPRRFVSRI LGMGDIESII EKIKGMEEYE QIQKKMEEVM SGKAKLTLRD IYKQLIALRK MGPLNKILQM LPGFGVFSQI PEEQLKLGEE KIKKFLVIMN SMTYKELDNP SIIDKSRIRR IAKGSGASPE EVKELLKQYQ VTNNLLKMVK RRKGLAKLFE DRNSK //