ID A0A5P2U4S2_KLULC Unreviewed; 236 AA. AC A0A5P2U4S2; DT 22-APR-2020, integrated into UniProtKB/TrEMBL. DT 22-APR-2020, sequence version 1. DT 03-AUG-2022, entry version 6. DE RecName: Full=2'-phosphotransferase {ECO:0000256|ARBA:ARBA00012007}; DE EC=2.7.1.160 {ECO:0000256|ARBA:ARBA00012007}; GN ORFNames=KDRO_C03230 {ECO:0000313|EMBL:QEU59801.1}; OS Kluyveromyces lactis (Yeast) (Candida sphaerica). OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; OC Saccharomycetales; Saccharomycetaceae; Kluyveromyces. OX NCBI_TaxID=28985 {ECO:0000313|EMBL:QEU59801.1, ECO:0000313|Proteomes:UP000323107}; RN [1] {ECO:0000313|Proteomes:UP000323107} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=CBS 2105 {ECO:0000313|Proteomes:UP000323107}; RA Ortiz-Merino R.A., Braun-Galleani S., Wolfe K.H., Morrissey J.P.; RT "Kluyveromyces lactis var. drosophilarum CBS 2105 Genome assembly."; RL Submitted (SEP-2019) to the EMBL/GenBank/DDBJ databases. CC -!- FUNCTION: Catalyzes the last step of tRNA splicing, the transfer of the CC splice junction 2'-phosphate from ligated tRNA to NAD to produce ADP- CC ribose 1''-2'' cyclic phosphate. {ECO:0000256|ARBA:ARBA00003343}. CC -!- CATALYTIC ACTIVITY: CC Reaction=2'-phospho-[ligated tRNA] + NAD(+) = ADP-beta-D-ribose CC 1'',2''-cyclic phosphate + mature tRNA + nicotinamide; CC Xref=Rhea:RHEA:23324, Rhea:RHEA-COMP:11106, Rhea:RHEA-COMP:11107, CC ChEBI:CHEBI:17154, ChEBI:CHEBI:57540, ChEBI:CHEBI:76596, CC ChEBI:CHEBI:82883, ChEBI:CHEBI:85027; EC=2.7.1.160; CC Evidence={ECO:0000256|ARBA:ARBA00001562}; CC -!- SIMILARITY: Belongs to the KptA/TPT1 family. CC {ECO:0000256|ARBA:ARBA00009836}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; CP042457; QEU59801.1; -; Genomic_DNA. DR VEuPathDB; FungiDB:KLLA0_C12397g; -. DR Proteomes; UP000323107; Chromosome 3. DR GO; GO:0000215; F:tRNA 2'-phosphotransferase activity; IEA:UniProtKB-EC. DR Gene3D; 1.10.10.970; -; 1. DR Gene3D; 3.20.170.30; -; 1. DR InterPro; IPR002745; Ptrans_KptA/Tpt1. DR InterPro; IPR042081; RNA_2'-PTrans_C. DR InterPro; IPR042080; RNA_2'-PTrans_N. DR PANTHER; PTHR12684; PTHR12684; 1. DR Pfam; PF01885; PTS_2-RNA; 1. PE 3: Inferred from homology; KW NAD {ECO:0000256|ARBA:ARBA00023027}; KW Transferase {ECO:0000256|ARBA:ARBA00022679}. SQ SEQUENCE 236 AA; 26314 MW; E86B45F1FB0E712D CRC64; MNTSHSQEPK DRSVKISKAL AYLLRHGAVK ENLPIDSDGY IALDRILQHN RLKSLKATST EIQHIVASND KQRFHIKTDP NSGLMYVCAT QGHTLKQIAP SQEVLKPITN IEQLSSKLVH GTNVSNCISI LQSGFIKKMS RNHVHLSPGI TGKDTDVISG MRNSSNVYIH IKKIQEMLDS LNLVKSLNNV YLTSQDIPLK FIDFIQVRKG RSAASSAQLQ QLKKLATEMN VPIEII //