ID A0A5N4AGB2_PHOPY Unreviewed; 4331 AA. AC A0A5N4AGB2; DT 26-FEB-2020, integrated into UniProtKB/TrEMBL. DT 26-FEB-2020, sequence version 1. DT 03-MAY-2023, entry version 15. DE RecName: Full=Carrier domain-containing protein {ECO:0000259|PROSITE:PS50075}; GN ORFNames=PPYR_10453 {ECO:0000313|EMBL:KAB0796392.1}; OS Photinus pyralis (Common eastern firefly) (Lampyris pyralis). OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Endopterygota; Coleoptera; Polyphaga; Elateriformia; Elateroidea; OC Lampyridae; Lampyrinae; Photinus. OX NCBI_TaxID=7054 {ECO:0000313|EMBL:KAB0796392.1, ECO:0000313|Proteomes:UP000327044}; RN [1] {ECO:0000313|EMBL:KAB0796392.1, ECO:0000313|Proteomes:UP000327044} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=1611_PpyrPB1 {ECO:0000313|EMBL:KAB0796392.1}; RC TISSUE=Whole body {ECO:0000313|EMBL:KAB0796392.1}; RX PubMed=30324905; RA Fallon T.R., Lower S.E., Chang C.H., Bessho-Uehara M., Martin G.J., RA Bewick A.J., Behringer M., Debat H.J., Wong I., Day J.C., Suvorov A., RA Silva C.J., Stanger-Hall K.F., Hall D.W., Schmitz R.J., Nelson D.R., RA Lewis S.M., Shigenobu S., Bybee S.M., Larracuente A.M., Oba Y., Weng J.K.; RT "Firefly genomes illuminate parallel origins of bioluminescence in RT beetles."; RL Elife 7:e36495-e36495(2018). CC -!- FUNCTION: Fatty acid synthetase is a multifunctional enzyme that CC catalyzes the de novo biosynthesis of long-chain saturated fatty acids CC starting from acetyl-CoA and malonyl-CoA in the presence of NADPH. This CC multifunctional protein contains 7 catalytic activities and a site for CC the binding of the prosthetic group 4'-phosphopantetheine of the acyl CC carrier protein ([ACP]) domain. {ECO:0000256|ARBA:ARBA00023442}. CC -!- CATALYTIC ACTIVITY: CC Reaction=(2E)-butenoyl-[ACP] + H(+) + NADPH = butanoyl-[ACP] + NADP(+); CC Xref=Rhea:RHEA:41812, Rhea:RHEA-COMP:9627, Rhea:RHEA-COMP:9628, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, CC ChEBI:CHEBI:78453, ChEBI:CHEBI:78454; CC Evidence={ECO:0000256|ARBA:ARBA00023357}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41813; CC Evidence={ECO:0000256|ARBA:ARBA00023357}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(2E)-decenoyl-[ACP] + H(+) + NADPH = decanoyl-[ACP] + NADP(+); CC Xref=Rhea:RHEA:41864, Rhea:RHEA-COMP:9639, Rhea:RHEA-COMP:9640, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, CC ChEBI:CHEBI:78467, ChEBI:CHEBI:78468; CC Evidence={ECO:0000256|ARBA:ARBA00023387}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41865; CC Evidence={ECO:0000256|ARBA:ARBA00023387}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(2E)-dodecenoyl-[ACP] + H(+) + NADPH = dodecanoyl-[ACP] + CC NADP(+); Xref=Rhea:RHEA:41880, Rhea:RHEA-COMP:9643, Rhea:RHEA- CC COMP:9644, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, CC ChEBI:CHEBI:65264, ChEBI:CHEBI:78472; CC Evidence={ECO:0000256|ARBA:ARBA00023385}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41881; CC Evidence={ECO:0000256|ARBA:ARBA00023385}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(2E)-hexadecenoyl-[ACP] + H(+) + NADPH = hexadecanoyl-[ACP] + CC NADP(+); Xref=Rhea:RHEA:41912, Rhea:RHEA-COMP:9651, Rhea:RHEA- CC COMP:9652, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, CC ChEBI:CHEBI:78481, ChEBI:CHEBI:78483; CC Evidence={ECO:0000256|ARBA:ARBA00023345}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41913; CC Evidence={ECO:0000256|ARBA:ARBA00023345}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(2E)-hexenoyl-[ACP] + H(+) + NADPH = hexanoyl-[ACP] + NADP(+); CC Xref=Rhea:RHEA:41832, Rhea:RHEA-COMP:9631, Rhea:RHEA-COMP:9632, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, CC ChEBI:CHEBI:78458, ChEBI:CHEBI:78459; CC Evidence={ECO:0000256|ARBA:ARBA00023359}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41833; CC Evidence={ECO:0000256|ARBA:ARBA00023359}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(2E)-octadecenoyl-[ACP] + H(+) + NADPH = NADP(+) + CC octadecanoyl-[ACP]; Xref=Rhea:RHEA:41928, Rhea:RHEA-COMP:9655, CC Rhea:RHEA-COMP:9656, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, CC ChEBI:CHEBI:58349, ChEBI:CHEBI:78489, ChEBI:CHEBI:78495; CC Evidence={ECO:0000256|ARBA:ARBA00023376}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41929; CC Evidence={ECO:0000256|ARBA:ARBA00023376}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(2E)-octenoyl-[ACP] + H(+) + NADPH = NADP(+) + octanoyl-[ACP]; CC Xref=Rhea:RHEA:41848, Rhea:RHEA-COMP:9635, Rhea:RHEA-COMP:9636, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, CC ChEBI:CHEBI:78462, ChEBI:CHEBI:78463; CC Evidence={ECO:0000256|ARBA:ARBA00023420}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41849; CC Evidence={ECO:0000256|ARBA:ARBA00023420}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(2E)-tetradecenoyl-[ACP] + H(+) + NADPH = NADP(+) + CC tetradecanoyl-[ACP]; Xref=Rhea:RHEA:41896, Rhea:RHEA-COMP:9647, CC Rhea:RHEA-COMP:9648, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, CC ChEBI:CHEBI:58349, ChEBI:CHEBI:78475, ChEBI:CHEBI:78477; CC Evidence={ECO:0000256|ARBA:ARBA00023368}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41897; CC Evidence={ECO:0000256|ARBA:ARBA00023368}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(3R)-hydroxybutanoyl-[ACP] = (2E)-butenoyl-[ACP] + H2O; CC Xref=Rhea:RHEA:41808, Rhea:RHEA-COMP:9626, Rhea:RHEA-COMP:9627, CC ChEBI:CHEBI:15377, ChEBI:CHEBI:78451, ChEBI:CHEBI:78453; CC Evidence={ECO:0000256|ARBA:ARBA00023402}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41809; CC Evidence={ECO:0000256|ARBA:ARBA00023402}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(3R)-hydroxydecanoyl-[ACP] = (2E)-decenoyl-[ACP] + H2O; CC Xref=Rhea:RHEA:41860, Rhea:RHEA-COMP:9638, Rhea:RHEA-COMP:9639, CC ChEBI:CHEBI:15377, ChEBI:CHEBI:78466, ChEBI:CHEBI:78467; CC Evidence={ECO:0000256|ARBA:ARBA00023388}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41861; CC Evidence={ECO:0000256|ARBA:ARBA00023388}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(3R)-hydroxydodecanoyl-[ACP] = (2E)-dodecenoyl-[ACP] + H2O; CC Xref=Rhea:RHEA:41876, Rhea:RHEA-COMP:9642, Rhea:RHEA-COMP:9643, CC ChEBI:CHEBI:15377, ChEBI:CHEBI:78470, ChEBI:CHEBI:78472; CC Evidence={ECO:0000256|ARBA:ARBA00023351}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41877; CC Evidence={ECO:0000256|ARBA:ARBA00023351}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(3R)-hydroxyhexadecanoyl-[ACP] = (2E)-hexadecenoyl-[ACP] + CC H2O; Xref=Rhea:RHEA:41908, Rhea:RHEA-COMP:9650, Rhea:RHEA-COMP:9651, CC ChEBI:CHEBI:15377, ChEBI:CHEBI:78480, ChEBI:CHEBI:78481; CC Evidence={ECO:0000256|ARBA:ARBA00023401}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41909; CC Evidence={ECO:0000256|ARBA:ARBA00023401}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(3R)-hydroxyhexanoyl-[ACP] = (2E)-hexenoyl-[ACP] + H2O; CC Xref=Rhea:RHEA:41828, Rhea:RHEA-COMP:9630, Rhea:RHEA-COMP:9631, CC ChEBI:CHEBI:15377, ChEBI:CHEBI:78457, ChEBI:CHEBI:78458; CC Evidence={ECO:0000256|ARBA:ARBA00023373}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41829; CC Evidence={ECO:0000256|ARBA:ARBA00023373}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(3R)-hydroxyoctadecanoyl-[ACP] = (2E)-octadecenoyl-[ACP] + CC H2O; Xref=Rhea:RHEA:41924, Rhea:RHEA-COMP:9654, Rhea:RHEA-COMP:9655, CC ChEBI:CHEBI:15377, ChEBI:CHEBI:78488, ChEBI:CHEBI:78489; CC Evidence={ECO:0000256|ARBA:ARBA00023399}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41925; CC Evidence={ECO:0000256|ARBA:ARBA00023399}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(3R)-hydroxyoctanoyl-[ACP] = (2E)-octenoyl-[ACP] + H2O; CC Xref=Rhea:RHEA:41844, Rhea:RHEA-COMP:9634, Rhea:RHEA-COMP:9635, CC ChEBI:CHEBI:15377, ChEBI:CHEBI:78461, ChEBI:CHEBI:78462; CC Evidence={ECO:0000256|ARBA:ARBA00023332}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41845; CC Evidence={ECO:0000256|ARBA:ARBA00023332}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(3R)-hydroxytetradecanoyl-[ACP] = (2E)-tetradecenoyl-[ACP] + CC H2O; Xref=Rhea:RHEA:41892, Rhea:RHEA-COMP:9646, Rhea:RHEA-COMP:9647, CC ChEBI:CHEBI:15377, ChEBI:CHEBI:78474, ChEBI:CHEBI:78475; CC Evidence={ECO:0000256|ARBA:ARBA00023398}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41893; CC Evidence={ECO:0000256|ARBA:ARBA00023398}; CC -!- CATALYTIC ACTIVITY: CC Reaction=3-oxobutanoyl-[ACP] + H(+) + NADPH = (3R)-hydroxybutanoyl- CC [ACP] + NADP(+); Xref=Rhea:RHEA:41804, Rhea:RHEA-COMP:9625, CC Rhea:RHEA-COMP:9626, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, CC ChEBI:CHEBI:58349, ChEBI:CHEBI:78450, ChEBI:CHEBI:78451; CC Evidence={ECO:0000256|ARBA:ARBA00023413}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41805; CC Evidence={ECO:0000256|ARBA:ARBA00023413}; CC -!- CATALYTIC ACTIVITY: CC Reaction=3-oxodecanoyl-[ACP] + H(+) + NADPH = (3R)-hydroxydecanoyl- CC [ACP] + NADP(+); Xref=Rhea:RHEA:41856, Rhea:RHEA-COMP:9637, CC Rhea:RHEA-COMP:9638, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, CC ChEBI:CHEBI:58349, ChEBI:CHEBI:78464, ChEBI:CHEBI:78466; CC Evidence={ECO:0000256|ARBA:ARBA00023418}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41857; CC Evidence={ECO:0000256|ARBA:ARBA00023418}; CC -!- CATALYTIC ACTIVITY: CC Reaction=3-oxododecanoyl-[ACP] + H(+) + NADPH = (3R)-hydroxydodecanoyl- CC [ACP] + NADP(+); Xref=Rhea:RHEA:41872, Rhea:RHEA-COMP:9641, CC Rhea:RHEA-COMP:9642, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, CC ChEBI:CHEBI:58349, ChEBI:CHEBI:78469, ChEBI:CHEBI:78470; CC Evidence={ECO:0000256|ARBA:ARBA00023416}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41873; CC Evidence={ECO:0000256|ARBA:ARBA00023416}; CC -!- CATALYTIC ACTIVITY: CC Reaction=3-oxohexadecanoyl-[ACP] + H(+) + NADPH = (3R)- CC hydroxyhexadecanoyl-[ACP] + NADP(+); Xref=Rhea:RHEA:41904, Rhea:RHEA- CC COMP:9649, Rhea:RHEA-COMP:9650, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, CC ChEBI:CHEBI:58349, ChEBI:CHEBI:78478, ChEBI:CHEBI:78480; CC Evidence={ECO:0000256|ARBA:ARBA00023390}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41905; CC Evidence={ECO:0000256|ARBA:ARBA00023390}; CC -!- CATALYTIC ACTIVITY: CC Reaction=3-oxohexanoyl-[ACP] + H(+) + NADPH = (3R)-hydroxyhexanoyl- CC [ACP] + NADP(+); Xref=Rhea:RHEA:41824, Rhea:RHEA-COMP:9629, CC Rhea:RHEA-COMP:9630, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, CC ChEBI:CHEBI:58349, ChEBI:CHEBI:78456, ChEBI:CHEBI:78457; CC Evidence={ECO:0000256|ARBA:ARBA00023346}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41825; CC Evidence={ECO:0000256|ARBA:ARBA00023346}; CC -!- CATALYTIC ACTIVITY: CC Reaction=3-oxooctadecanoyl-[ACP] + H(+) + NADPH = (3R)- CC hydroxyoctadecanoyl-[ACP] + NADP(+); Xref=Rhea:RHEA:41920, Rhea:RHEA- CC COMP:9653, Rhea:RHEA-COMP:9654, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, CC ChEBI:CHEBI:58349, ChEBI:CHEBI:78487, ChEBI:CHEBI:78488; CC Evidence={ECO:0000256|ARBA:ARBA00023419}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41921; CC Evidence={ECO:0000256|ARBA:ARBA00023419}; CC -!- CATALYTIC ACTIVITY: CC Reaction=3-oxooctanoyl-[ACP] + H(+) + NADPH = (3R)-hydroxyoctanoyl- CC [ACP] + NADP(+); Xref=Rhea:RHEA:41840, Rhea:RHEA-COMP:9633, CC Rhea:RHEA-COMP:9634, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, CC ChEBI:CHEBI:58349, ChEBI:CHEBI:78460, ChEBI:CHEBI:78461; CC Evidence={ECO:0000256|ARBA:ARBA00023364}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41841; CC Evidence={ECO:0000256|ARBA:ARBA00023364}; CC -!- CATALYTIC ACTIVITY: CC Reaction=3-oxotetradecanoyl-[ACP] + H(+) + NADPH = (3R)- CC hydroxytetradecanoyl-[ACP] + NADP(+); Xref=Rhea:RHEA:41888, CC Rhea:RHEA-COMP:9645, Rhea:RHEA-COMP:9646, ChEBI:CHEBI:15378, CC ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:78473, CC ChEBI:CHEBI:78474; Evidence={ECO:0000256|ARBA:ARBA00023367}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41889; CC Evidence={ECO:0000256|ARBA:ARBA00023367}; CC -!- CATALYTIC ACTIVITY: CC Reaction=H(+) + hexadecanoyl-[ACP] + malonyl-[ACP] = 3-oxooctadecanoyl- CC [ACP] + CO2 + holo-[ACP]; Xref=Rhea:RHEA:41916, Rhea:RHEA-COMP:9623, CC Rhea:RHEA-COMP:9652, Rhea:RHEA-COMP:9653, Rhea:RHEA-COMP:9685, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:64479, CC ChEBI:CHEBI:78449, ChEBI:CHEBI:78483, ChEBI:CHEBI:78487; CC Evidence={ECO:0000256|ARBA:ARBA00023365}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41917; CC Evidence={ECO:0000256|ARBA:ARBA00023365}; CC -!- CATALYTIC ACTIVITY: CC Reaction=H(+) + hexanoyl-[ACP] + malonyl-[ACP] = 3-oxooctanoyl-[ACP] + CC CO2 + holo-[ACP]; Xref=Rhea:RHEA:41836, Rhea:RHEA-COMP:9623, CC Rhea:RHEA-COMP:9632, Rhea:RHEA-COMP:9633, Rhea:RHEA-COMP:9685, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:64479, CC ChEBI:CHEBI:78449, ChEBI:CHEBI:78459, ChEBI:CHEBI:78460; CC Evidence={ECO:0000256|ARBA:ARBA00023361}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41837; CC Evidence={ECO:0000256|ARBA:ARBA00023361}; CC -!- CATALYTIC ACTIVITY: CC Reaction=H(+) + malonyl-[ACP] + octanoyl-[ACP] = 3-oxodecanoyl-[ACP] + CC CO2 + holo-[ACP]; Xref=Rhea:RHEA:41852, Rhea:RHEA-COMP:9623, CC Rhea:RHEA-COMP:9636, Rhea:RHEA-COMP:9637, Rhea:RHEA-COMP:9685, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:64479, CC ChEBI:CHEBI:78449, ChEBI:CHEBI:78463, ChEBI:CHEBI:78464; CC Evidence={ECO:0000256|ARBA:ARBA00023414}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41853; CC Evidence={ECO:0000256|ARBA:ARBA00023414}; CC -!- CATALYTIC ACTIVITY: CC Reaction=H(+) + malonyl-[ACP] + tetradecanoyl-[ACP] = 3- CC oxohexadecanoyl-[ACP] + CO2 + holo-[ACP]; Xref=Rhea:RHEA:41900, CC Rhea:RHEA-COMP:9623, Rhea:RHEA-COMP:9648, Rhea:RHEA-COMP:9649, CC Rhea:RHEA-COMP:9685, ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, CC ChEBI:CHEBI:64479, ChEBI:CHEBI:78449, ChEBI:CHEBI:78477, CC ChEBI:CHEBI:78478; Evidence={ECO:0000256|ARBA:ARBA00023341}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41901; CC Evidence={ECO:0000256|ARBA:ARBA00023341}; CC -!- CATALYTIC ACTIVITY: CC Reaction=H2O + hexadecanoyl-[ACP] = H(+) + hexadecanoate + holo-[ACP]; CC Xref=Rhea:RHEA:41932, Rhea:RHEA-COMP:9652, Rhea:RHEA-COMP:9685, CC ChEBI:CHEBI:7896, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, CC ChEBI:CHEBI:64479, ChEBI:CHEBI:78483; EC=3.1.2.14; CC Evidence={ECO:0000256|ARBA:ARBA00023378}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41933; CC Evidence={ECO:0000256|ARBA:ARBA00023378}; CC -!- CATALYTIC ACTIVITY: CC Reaction=H2O + tetradecanoyl-[ACP] = H(+) + holo-[ACP] + CC tetradecanoate; Xref=Rhea:RHEA:30123, Rhea:RHEA-COMP:9648, Rhea:RHEA- CC COMP:9685, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30807, CC ChEBI:CHEBI:64479, ChEBI:CHEBI:78477; EC=3.1.2.14; CC Evidence={ECO:0000256|ARBA:ARBA00023410}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:30124; CC Evidence={ECO:0000256|ARBA:ARBA00023410}; CC -!- CATALYTIC ACTIVITY: CC Reaction=a (3R)-hydroxyacyl-[ACP] + NADP(+) = a 3-oxoacyl-[ACP] + H(+) CC + NADPH; Xref=Rhea:RHEA:17397, Rhea:RHEA-COMP:9916, Rhea:RHEA- CC COMP:9945, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, CC ChEBI:CHEBI:78776, ChEBI:CHEBI:78827; EC=1.1.1.100; CC Evidence={ECO:0000256|ARBA:ARBA00023333}; CC PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:17399; CC Evidence={ECO:0000256|ARBA:ARBA00023333}; CC -!- CATALYTIC ACTIVITY: CC Reaction=a (3R)-hydroxyacyl-[ACP] = a (2E)-enoyl-[ACP] + H2O; CC Xref=Rhea:RHEA:13097, Rhea:RHEA-COMP:9925, Rhea:RHEA-COMP:9945, CC ChEBI:CHEBI:15377, ChEBI:CHEBI:78784, ChEBI:CHEBI:78827; EC=4.2.1.59; CC Evidence={ECO:0000256|ARBA:ARBA00023394}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:13098; CC Evidence={ECO:0000256|ARBA:ARBA00023394}; CC -!- CATALYTIC ACTIVITY: CC Reaction=a 2,3-saturated acyl-[ACP] + NADP(+) = a (2E)-enoyl-[ACP] + CC H(+) + NADPH; Xref=Rhea:RHEA:22564, Rhea:RHEA-COMP:9925, Rhea:RHEA- CC COMP:9926, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, CC ChEBI:CHEBI:78784, ChEBI:CHEBI:78785; EC=1.3.1.39; CC Evidence={ECO:0000256|ARBA:ARBA00023381}; CC PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:22566; CC Evidence={ECO:0000256|ARBA:ARBA00023381}; CC -!- CATALYTIC ACTIVITY: CC Reaction=a fatty acyl-[ACP] + H(+) + malonyl-[ACP] = a 3-oxoacyl-[ACP] CC + CO2 + holo-[ACP]; Xref=Rhea:RHEA:22836, Rhea:RHEA-COMP:9623, CC Rhea:RHEA-COMP:9685, Rhea:RHEA-COMP:9916, Rhea:RHEA-COMP:14125, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:64479, CC ChEBI:CHEBI:78449, ChEBI:CHEBI:78776, ChEBI:CHEBI:138651; CC EC=2.3.1.41; Evidence={ECO:0000256|ARBA:ARBA00023389}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:22837; CC Evidence={ECO:0000256|ARBA:ARBA00023389}; CC -!- CATALYTIC ACTIVITY: CC Reaction=acetyl-CoA + 2n H(+) + n malonyl-CoA + 2n NADPH = a long-chain CC fatty acid + n CO2 + (n+1) CoA + 2n NADP(+); Xref=Rhea:RHEA:14993, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57287, CC ChEBI:CHEBI:57288, ChEBI:CHEBI:57384, ChEBI:CHEBI:57560, CC ChEBI:CHEBI:57783, ChEBI:CHEBI:58349; EC=2.3.1.85; CC Evidence={ECO:0000256|ARBA:ARBA00023370}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:14994; CC Evidence={ECO:0000256|ARBA:ARBA00023370}; CC -!- CATALYTIC ACTIVITY: CC Reaction=acetyl-CoA + holo-[ACP] = acetyl-[ACP] + CoA; CC Xref=Rhea:RHEA:41788, Rhea:RHEA-COMP:9621, Rhea:RHEA-COMP:9685, CC ChEBI:CHEBI:57287, ChEBI:CHEBI:57288, ChEBI:CHEBI:64479, CC ChEBI:CHEBI:78446; EC=2.3.1.38; CC Evidence={ECO:0000256|ARBA:ARBA00023397}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41789; CC Evidence={ECO:0000256|ARBA:ARBA00023397}; CC -!- CATALYTIC ACTIVITY: CC Reaction=acetyl-[ACP] + H(+) + malonyl-[ACP] = 3-oxobutanoyl-[ACP] + CC CO2 + holo-[ACP]; Xref=Rhea:RHEA:41800, Rhea:RHEA-COMP:9621, CC Rhea:RHEA-COMP:9623, Rhea:RHEA-COMP:9625, Rhea:RHEA-COMP:9685, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:64479, CC ChEBI:CHEBI:78446, ChEBI:CHEBI:78449, ChEBI:CHEBI:78450; CC Evidence={ECO:0000256|ARBA:ARBA00023352}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41801; CC Evidence={ECO:0000256|ARBA:ARBA00023352}; CC -!- CATALYTIC ACTIVITY: CC Reaction=butanoyl-[ACP] + H(+) + malonyl-[ACP] = 3-oxohexanoyl-[ACP] + CC CO2 + holo-[ACP]; Xref=Rhea:RHEA:41820, Rhea:RHEA-COMP:9623, CC Rhea:RHEA-COMP:9628, Rhea:RHEA-COMP:9629, Rhea:RHEA-COMP:9685, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:64479, CC ChEBI:CHEBI:78449, ChEBI:CHEBI:78454, ChEBI:CHEBI:78456; CC Evidence={ECO:0000256|ARBA:ARBA00023403}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41821; CC Evidence={ECO:0000256|ARBA:ARBA00023403}; CC -!- CATALYTIC ACTIVITY: CC Reaction=decanoyl-[ACP] + H(+) + malonyl-[ACP] = 3-oxododecanoyl-[ACP] CC + CO2 + holo-[ACP]; Xref=Rhea:RHEA:41868, Rhea:RHEA-COMP:9623, CC Rhea:RHEA-COMP:9640, Rhea:RHEA-COMP:9641, Rhea:RHEA-COMP:9685, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:64479, CC ChEBI:CHEBI:78449, ChEBI:CHEBI:78468, ChEBI:CHEBI:78469; CC Evidence={ECO:0000256|ARBA:ARBA00023396}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41869; CC Evidence={ECO:0000256|ARBA:ARBA00023396}; CC -!- CATALYTIC ACTIVITY: CC Reaction=dodecanoyl-[ACP] + H(+) + malonyl-[ACP] = 3-oxotetradecanoyl- CC [ACP] + CO2 + holo-[ACP]; Xref=Rhea:RHEA:41884, Rhea:RHEA-COMP:9623, CC Rhea:RHEA-COMP:9644, Rhea:RHEA-COMP:9645, Rhea:RHEA-COMP:9685, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:64479, CC ChEBI:CHEBI:65264, ChEBI:CHEBI:78449, ChEBI:CHEBI:78473; CC Evidence={ECO:0000256|ARBA:ARBA00023348}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41885; CC Evidence={ECO:0000256|ARBA:ARBA00023348}; CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ CC whole genome shotgun (WGS) entry which is preliminary data. CC {ECO:0000313|EMBL:KAB0796392.1}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; VVIM01000007; KAB0796392.1; -; Genomic_DNA. DR Proteomes; UP000327044; Unassembled WGS sequence. DR GO; GO:0019171; F:(3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase activity; IEA:UniProtKB-EC. DR GO; GO:0008659; F:(3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase activity; IEA:UniProtKB-EC. DR GO; GO:0004316; F:3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity; IEA:UniProtKB-EC. DR GO; GO:0004315; F:3-oxoacyl-[acyl-carrier-protein] synthase activity; IEA:UniProtKB-EC. DR GO; GO:0004313; F:[acyl-carrier-protein] S-acetyltransferase activity; IEA:UniProtKB-EC. DR GO; GO:0047117; F:enoyl-[acyl-carrier-protein] reductase (NADPH, A-specific) activity; IEA:UniProtKB-EC. DR GO; GO:0016295; F:myristoyl-[acyl-carrier-protein] hydrolase activity; IEA:UniProtKB-EC. DR GO; GO:0004320; F:oleoyl-[acyl-carrier-protein] hydrolase activity; IEA:UniProtKB-EC. DR GO; GO:0016296; F:palmitoyl-[acyl-carrier-protein] hydrolase activity; IEA:UniProtKB-EC. DR GO; GO:0031177; F:phosphopantetheine binding; IEA:InterPro. DR GO; GO:0006633; P:fatty acid biosynthetic process; IEA:UniProtKB-KW. DR CDD; cd05195; enoyl_red; 2. DR CDD; cd08954; KR_1_FAS_SDR_x; 2. DR CDD; cd00833; PKS; 1. DR Gene3D; 3.30.70.3290; -; 5. DR Gene3D; 3.40.47.10; -; 4. DR Gene3D; 1.10.1200.10; ACP-like; 1. DR Gene3D; 3.40.50.1820; alpha/beta hydrolase; 1. DR Gene3D; 3.40.366.10; Malonyl-Coenzyme A Acyl Carrier Protein, domain 2; 3. DR Gene3D; 3.90.180.10; Medium-chain alcohol dehydrogenases, catalytic domain; 3. DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 2. DR Gene3D; 3.10.129.110; Polyketide synthase dehydratase; 3. DR InterPro; IPR029058; AB_hydrolase. DR InterPro; IPR001227; Ac_transferase_dom_sf. DR InterPro; IPR036736; ACP-like_sf. DR InterPro; IPR016035; Acyl_Trfase/lysoPLipase. DR InterPro; IPR011032; GroES-like_sf. DR InterPro; IPR018201; Ketoacyl_synth_AS. DR InterPro; IPR014031; Ketoacyl_synth_C. DR InterPro; IPR014030; Ketoacyl_synth_N. DR InterPro; IPR036291; NAD(P)-bd_dom_sf. DR InterPro; IPR032821; PKS_assoc. DR InterPro; IPR020841; PKS_Beta-ketoAc_synthase_dom. DR InterPro; IPR042104; PKS_dehydratase_sf. DR InterPro; IPR020807; PKS_DH. DR InterPro; IPR020843; PKS_ER. DR InterPro; IPR013968; PKS_KR. DR InterPro; IPR020806; PKS_PP-bd. DR InterPro; IPR009081; PP-bd_ACP. DR InterPro; IPR001031; Thioesterase. DR InterPro; IPR016039; Thiolase-like. DR PANTHER; PTHR43775:SF23; 3-OXOACYL-[ACYL-CARRIER-PROTEIN] REDUCTASE; 1. DR PANTHER; PTHR43775; FATTY ACID SYNTHASE; 1. DR Pfam; PF13602; ADH_zinc_N_2; 2. DR Pfam; PF16197; KAsynt_C_assoc; 2. DR Pfam; PF00109; ketoacyl-synt; 3. DR Pfam; PF02801; Ketoacyl-synt_C; 2. DR Pfam; PF08659; KR; 3. DR Pfam; PF00550; PP-binding; 1. DR Pfam; PF14765; PS-DH; 3. DR Pfam; PF00975; Thioesterase; 1. DR SMART; SM00829; PKS_ER; 2. DR SMART; SM00822; PKS_KR; 2. DR SMART; SM00825; PKS_KS; 1. DR SMART; SM00823; PKS_PP; 1. DR SUPFAM; SSF47336; ACP-like; 1. DR SUPFAM; SSF53474; alpha/beta-Hydrolases; 1. DR SUPFAM; SSF52151; FabD/lysophospholipase-like; 3. DR SUPFAM; SSF50129; GroES-like; 2. DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 5. DR SUPFAM; SSF53901; Thiolase-like; 3. DR PROSITE; PS00606; B_KETOACYL_SYNTHASE; 1. DR PROSITE; PS50075; CARRIER; 1. PE 4: Predicted; KW Fatty acid biosynthesis {ECO:0000256|ARBA:ARBA00023160}; KW Fatty acid metabolism {ECO:0000256|ARBA:ARBA00023160}; KW Lipid biosynthesis {ECO:0000256|ARBA:ARBA00022516}; KW Lipid metabolism {ECO:0000256|ARBA:ARBA00023160}; KW Multifunctional enzyme {ECO:0000256|ARBA:ARBA00023268}; KW Phosphopantetheine {ECO:0000256|ARBA:ARBA00022450}; KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553}; KW Pyridoxal phosphate {ECO:0000256|ARBA:ARBA00022898}; KW Reference proteome {ECO:0000313|Proteomes:UP000327044}; KW S-nitrosylation {ECO:0000256|ARBA:ARBA00022799}; KW Transferase {ECO:0000256|ARBA:ARBA00022679}. FT DOMAIN 3964..4041 FT /note="Carrier" FT /evidence="ECO:0000259|PROSITE:PS50075" SQ SEQUENCE 4331 AA; 482670 MW; B9D1450A2449B4BB CRC64; MSALSKANLR APGHRQWTLQ IFNVFRDRTE NRLTTTAPID VIKIIKNCEC KDGRELVLAS LAIQLAFVEL LKELNLSLDY TAGMSVGELS AGYANGRINF EQAIEVGYEI SRLMSDDLCL DYALSTKSSV LLSRLQKRIP NPEGIIHSLL NPKPLEARVL QRKHYPQLAV VVLGSQQIRL NGFTSLNISG SDVVTSFLDA VGSIYMLGHN LNVEVLYPPV KWPVGRGTPM ISPLIKWNYD IDWLSSFHSY SKKVWYADSH LITLVSKEID WSILPGHVID GMTIMPAIGY LFMIWETYCK MVDVPMNISR VRFDNVRFLK AVHITKLSEM AFKVTIGRGT NRFEIGDVDE VIVSGSIRTL SDTDEFIDLA LSPEDDEGIS FATKDIYKEL RLRGYNYSGA FRAQETAHIT GLISWIKWDR NWIAFIDNML QMKLLGEDTR NLYVPTFIGH LKIDAMKHFE YVKQLDNPSL LPVYNHREAD AISCGGIELR KLYATPIMRR KVYASSINFM NIMLASGRVP SEAFIKDRLA LATAQGVEYA GIDAKGERVM GILRLGAMAS SVVPDGNMTW RIPDEWTLSQ SASVPITYST VLFSCDPSMT YIILGGLGGF GLELVDWLIL RGARKLVLVS RNGVRNGYAA SRIRVWKMYE ATIRVSTEDV TIEEGCARLL ESSEKLAPVA GIFNLAVVLQ DDLFENQKEE NFVNTLSPKA RLHYHEANQM DPMVKITLEK AYEAIVDAGY NESEMDGADC GVFIGCCFSE SEEVVLMATI ASPNFGVTGS SLYALEHAYR AIRTGRCDSA LVGGVSLCLN PTTSLQFFRP LIVGSVKSYL GHPEGASGMC SLVKVLVGME DDCILPNINY NKPRQSIKAL VDGRMVVPVD RTSWPENGSG VVAINCFGFG GSNAHLILKR FEQRKVNGGL PEDDLPRLIC VSGRAPESLK AIFDDIGGRK LDAEYCRILQ EVFKSPNFEH PYRGYIIHSK IGELKRSSTR FDSKSRVCLL LPRTSLELLE IGSRLFKFSA FSATVQRIAS LLPPQVDISK IIKNCECNSG RELVLANLGI QLAFIELLKE LNLSLDYTGG MSIGELSAGY ANGRINLEQA LAVGYEISRL VSDDLRLEHA ISTKSSVLLT RLQKVLPHPE VILHSLLNPK PLEERLLQQS RYPDLAVVVL GSDQIRLNGF TFLHISSLDV VTSFLDVVGS LYMLGHNLNV EALYPPVKWP VSRGTPMISP LIKWNYDMDW FTKLHTKKVL HKGINVIPLV NKEIDWTILP GHVIDGMTIM PATGYLFMVW ETYCKIVDVP IAGSRVRFDN VRFHKAVHIS KISEMEFKII IGRGTNRFEI ADADEVIVSG SIHTLSETDE FIDLALSPED DEGISFASKD IYKELRLRGY NYSGAFRAQE ESHISGLISW IKWDRNWIAF IDNMLQTKLL GEDTRSLYVP TFIGHLKIDA MKHFEYVKQL DNPSLLPVYN HREADAISCG GIELRKLYAT PILRRKVTAE PVLETHKFVP FDTELPMQSA VRVLVQIFLE NTLSLKVKIV EVAQTDAIAP ILQEALDDQP LVKTEVTILG DENLTAPNLK VHNKALSAEK KCAIVILEGA SGNLKLLTEA KGALKENGII ISREKGGFAS NFPGLAILAQ VKTEMETLVL LRKVVGYAKE HSAHLKPGQS LLIHSGAGGV GQAAIHIALF YKCQIFTTVG TEEKRKFIME TFPEIPASHI GNSRDTSFVQ MVLKHTKGRG VDLVLNSLAE AKLKATVQCL APKGYFVEIG KYDLVNSSKL ALSVLSEGRR YAGCMLDMYF RDFHNQKHTL QQILSKFLKQ GAVKPLSLTT FNMDQVEEAY RYMAAGKHIG KVVVKVRDEN QQSPELFKAL PRFSCDPCMT YIILGGLGGF GLELVDWLIL RGARKLVLVS RNGVRNGYAA SRLRAWKTYE AVVKVATEDV TTEKGCARLL ESSEKLAPVA AIFNLAVVLR DDLFENQTEE NFRNTLSPKA RATQHLDTLS RQHCPRLQHF VVFSSVSCGR GNAGQTTYGM ANSIMERICE KRKREGYPGL AIQWGAIGEV GLVAEMQEQH KEIVISGTLQ QRVASCLSTL ETFLLQNEPI VASMVVAEKK SIAEGAENVI NAIRSIMRMS GRFPESDDIY QYMRNIYNKF DMITSDNRRW LPTNPEIPHR IGKISHLEKL DAGQFDLHYH EANQMDPMIK ISLEKAYEAI LDAGYNVNEM DGANCGVFMG CCFSESEEVV LMATNASPNF GVTGSMTSQR ISHYFNLKGP SYITDTACSS SLYALEHAYR AIRTGRCESA LVGGVSLCLN PIASLQFARL GVLSADGMCK PFDEDAKGYA RSETVSVVFL QKAKDARRIY AQLLHSKTNS DGYKEQGITF PSSEMQKVLL DDTYEECRLD PTTIDFLEAH ATGTRVGDPE EMAAMEQVFC TNRDTPLIAG SIKSYLGHAE GASGMCSLVK VLIGMEDDCL LPNIHYDKPR QSIKGLVDGR VVVPLDKMLW PKNGSGVVAI NCFGFGGSNA HVILKRFEQR KINEGLPDDD LPRLMCVSGR VPESLKSIFD DISGRKLDAE YCRLLQEAFK GTTTDHPYRG YIIHSKAGEL KRSSTRFDSK RRVCLLLPGP TMELLEIGSG LFRFSAFSAA VERIASLLPP EVHLTKIIKN CECNCGRELV LANLAIQLAF IELFKELNLS LDYTAGMSIG ELSAGYANGR INLEQAIAVG YEISRLVGDD LRLEYALSTK SSLLLSRLQK VIPSPEVMLH SLLNPKPLEA REWQQSHYPE LAVVVLGSDR IRLNGFASLH ISGSDAVTSF LDVVGGLYKL GHNLNVEALY PPVKWPVGRG TPMISPLIKW NYDLDWFTKL HTKTVLHTGA QVIPLVNKTI DWSILPGHVI DGMTIMPATG YLFMVWETYC KIVDVPIAGS RVRFDNVRFH KAVHISKISE MEFKITIGRG TNRFEIGDVD EVIVSGSIRT LSETDEFIDL ALSPEDDEGI SFASKDIYKE LRLRGYNYSG AFRSQEKAHI SGLISWIKWE RNWIAFIDNM LQTKLLGEDT RSLYVPTFIG QLKIDAMKHF EYVKELDNPS LLPVYNHREA DAISCGGIEL RKLYATSILR RKVTAEPVLE THKFVPFNTE LPMQSAVRVL VQIFLENSLS LKVKIVEVAQ TDAIAPILQE ALNDQPLVKT EVIILGDENL SVPNMKVHNK ALSTEKQCAI VIAEEASGNL ELLKEAEGVL KENGIIISRE RGEFTANFPG LALLAQVKTE TDTLVLLRKV VSYPKEHVIM AKFDGTTYDW LPNLQSAMRN ETKTLVVVEN QPLSGVLGFV NCLRREPGGT NVKCVFVQNG NIKFNLSDPL FREQLDKDLA INVLNQDVWG SYRHLPLERV TEVETQHVFC NQNVVGNLST LSWVEGLPSK DPERSIKVYA SSINFMNIML ASGRVPSEAY TKDRLISTSA QGIEYAGIDA RGERVMGIQY GAMASYVVPD GEIMWRIPCE WTMDQGVSIP ITYSTVLCSF FVSAHLKPGQ SLLIHSGAGG VGQAAIYIAL FYKCQIFTTV GTEEKRKFIM ETFPEIPANH IGNSRDTSFV QMVLKHTRGR GVDLVLNSLA EEKLKASLQC LAPKGYFVEI GKYDLVNSNT LALSVLSEGR RYAGCMLDMY LGDCHNQKHK LQQILSKFLK QGAVKPLFLA TFNMDQAEEA YRFMAAGKHV GKVVIKVRDE NQQSPKLFKA LPRFSCDPCM TYIILGGLGG FGLELVDWLI LRGARKLVLV SRNGVRNGYA ASRLRTWKTY EATIRVATDD VTTEEGCARL LESSEKLAPV AAIFNLAVVL RDDLFENQTE ENFVNALSPK ARATQHLDIL SRQLCPRLQH FVVFSSVSCG RGNAGQTTYG MANSIMERIC EKRKREGYPG LAIQWGAIGE VGLVAEMQEQ HKEIVISGTL QQRVASCLST LETFLLQNEP IVASMVVAEK KSVAEGAESV ISAIKGIMGI TDMTTISEHA TLSEMGMDSM TAVEIKQTLE RDCEVFLSPK EIRTLTFAKL KEKLSKEASD PVAPRLQPRS NIFFVHFRTE EDSKIPLKRL ESLVKKEDQA PLAIFLPGIE GVAETMEFLA KNLHSHVECI QYANNVSDFR LEAFAKSLPT LIPHVKNHFN LVAYSYGCVV ALELASILEV RGLIGKVILI DGAPEMLKNL IKLQYPSEHE SEAFLETAIL FNLITSYAPM EATVELRETV RQLRTLKERV EYLLEVIPEG IPHSKDYQRQ VGIAVCAKTK SVYNYEANFP KLKSEVTLIK PNKSPFVNYD EDYRLQKLCE KPVRVHTIEG THTTIVQNPE LADCINKIIS N //