ID A0A5M9WIK3_9LACO Unreviewed; 410 AA. AC A0A5M9WIK3; DT 26-FEB-2020, integrated into UniProtKB/TrEMBL. DT 26-FEB-2020, sequence version 1. DT 22-APR-2020, entry version 2. DE RecName: Full=Cysteine desulfurase {ECO:0000256|SAAS:SAAS00062216}; DE EC=2.8.1.7 {ECO:0000256|SAAS:SAAS00062216}; GN ORFNames=F1C01_04695 {ECO:0000313|EMBL:KAA8781180.1}; OS Lactobacillus crispatus. OC Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae; OC Lactobacillus. OX NCBI_TaxID=47770 {ECO:0000313|EMBL:KAA8781180.1, ECO:0000313|Proteomes:UP000324846}; RN [1] {ECO:0000313|EMBL:KAA8781180.1, ECO:0000313|Proteomes:UP000324846} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=NCK971 {ECO:0000313|EMBL:KAA8781180.1, RC ECO:0000313|Proteomes:UP000324846}; RA Pan M., Hidalgo-Cantabrana C., Barrangou R.; RT "Host and body site-specific adaptation of Lactobacillus crispatus RT genomes."; RL Submitted (SEP-2019) to the EMBL/GenBank/DDBJ databases. CC -!- CATALYTIC ACTIVITY: CC Reaction=[sulfur carrier]-H + L-cysteine = [sulfur carrier]-SH + L- CC alanine; Xref=Rhea:RHEA:43892, Rhea:RHEA-COMP:14737, Rhea:RHEA- CC COMP:14739, ChEBI:CHEBI:29917, ChEBI:CHEBI:35235, ChEBI:CHEBI:57972, CC ChEBI:CHEBI:64428; EC=2.8.1.7; CC Evidence={ECO:0000256|SAAS:SAAS01122505}; CC -!- COFACTOR: CC Name=pyridoxal 5'-phosphate; Xref=ChEBI:CHEBI:597326; CC Evidence={ECO:0000256|SAAS:SAAS00608953}; CC -!- SIMILARITY: Belongs to the class-V pyridoxal-phosphate-dependent CC aminotransferase family. {ECO:0000256|SAAS:SAAS00549899}. CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ CC whole genome shotgun (WGS) entry which is preliminary data. CC {ECO:0000313|EMBL:KAA8781180.1}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; VYYV01000001; KAA8781180.1; -; Genomic_DNA. DR RefSeq; WP_065990616.1; NZ_VYYV01000001.1. DR Proteomes; UP000324846; Unassembled WGS sequence. DR Gene3D; 3.40.640.10; -; 1. DR Gene3D; 3.90.1150.10; -; 1. DR InterPro; IPR000192; Aminotrans_V_dom. DR InterPro; IPR010970; Cys_dSase_SufS. DR InterPro; IPR016454; Cysteine_dSase. DR InterPro; IPR015424; PyrdxlP-dep_Trfase. DR InterPro; IPR015422; PyrdxlP-dep_Trfase_dom1. DR InterPro; IPR015421; PyrdxlP-dep_Trfase_major. DR Pfam; PF00266; Aminotran_5; 1. DR PIRSF; PIRSF005572; NifS; 1. DR SUPFAM; SSF53383; SSF53383; 1. DR TIGRFAMs; TIGR01979; sufS; 1. PE 3: Inferred from homology; KW Pyridoxal phosphate {ECO:0000256|SAAS:SAAS00184662}; KW Transferase {ECO:0000256|SAAS:SAAS00062250}. FT DOMAIN 27..395 FT /note="Aminotran_5" FT /evidence="ECO:0000259|Pfam:PF00266" SQ SEQUENCE 410 AA; 45742 MW; 4BDAB11010393FC2 CRC64; MDKEAITKIR NDFPILKQKI NGNRLAYLDN AATMQMPVPI LHKINSFYQT SYANVHRSVD TLGFNATKQY EEARGKVASF INAADANEII FTSGCTDSLN LVAATYGEQN IHEQDEIVLT IMEHHSNLLP WQQLAKRKGA ILKYIDLTES QTLDLDDVKK KITAKTKIVA IAHASNILGM MNPIEEVIKI AHQVEAVVVV DGAQAVSHFP IDVQKLDADF YAFSGHKMLA PTGIGVLYGK KNLLNQMPPY RFGGEMIGNV TKYNATWADL PQKFEAGTPN IIGAIGLGYA IDYLHEIGMN RIQTYENELS KYLMQKIEKL DFVAIYGPKE NHTGVFSFNL KNIHPHDVAT ALDMQGIEVR AGHHCAQPLM RRLGVESTVR ASLSFYNNKE DIDQLIAGIS ETEEFFNGTK //