ID A0A5I2WV80_SALET Unreviewed; 257 AA. AC A0A5I2WV80; DT 11-DEC-2019, integrated into UniProtKB/TrEMBL. DT 11-DEC-2019, sequence version 1. DT 07-OCT-2020, entry version 6. DE RecName: Full=NADH pyrophosphatase {ECO:0000256|ARBA:ARBA00021298, ECO:0000256|HAMAP-Rule:MF_00297}; DE EC=3.6.1.22 {ECO:0000256|ARBA:ARBA00012381, ECO:0000256|HAMAP-Rule:MF_00297}; GN Name=nudC {ECO:0000256|HAMAP-Rule:MF_00297, GN ECO:0000313|EMBL:ECF3836160.1}; GN ORFNames=A3X66_20740 {ECO:0000313|EMBL:ECS2758674.1}, APM01_23080 GN {ECO:0000313|EMBL:ECS2231151.1}, APN55_22505 GN {ECO:0000313|EMBL:ECT4987962.1}, APN76_22850 GN {ECO:0000313|EMBL:ECU5519599.1}, AR812_23430 GN {ECO:0000313|EMBL:EBW0682999.1}, BFV08_22450 GN {ECO:0000313|EMBL:ECT7930094.1}, D4T73_23110 GN {ECO:0000313|EMBL:ECT3946125.1}, D8Z14_22610 GN {ECO:0000313|EMBL:EBZ2411064.1}, DC274_21995 GN {ECO:0000313|EMBL:ECV5544209.1}, DM677_23175 GN {ECO:0000313|EMBL:EBU9895995.1}, DN927_22070 GN {ECO:0000313|EMBL:ECT0134668.1}, DOG83_22505 GN {ECO:0000313|EMBL:ECV3391360.1}, DPE57_22935 GN {ECO:0000313|EMBL:EBS3934698.1}, DQK10_24005 GN {ECO:0000313|EMBL:MLO07687.1}, DWS46_21290 GN {ECO:0000313|EMBL:ECV1784185.1}, DYO32_22160 GN {ECO:0000313|EMBL:ECB8738000.1}, DYS13_21830 GN {ECO:0000313|EMBL:ECV1599414.1}, DYT81_20305 GN {ECO:0000313|EMBL:ECT2693329.1}, E1821_21895 GN {ECO:0000313|EMBL:ECG8287559.1}, E1B31_21850 GN {ECO:0000313|EMBL:ECD4765434.1}, E4A02_22065 GN {ECO:0000313|EMBL:ECF3836160.1}, E5908_22465 GN {ECO:0000313|EMBL:ECB8847373.1}, EUR83_22525 GN {ECO:0000313|EMBL:ECB0315133.1}, EWC91_21850 GN {ECO:0000313|EMBL:ECG2745078.1}, EWI81_21765 GN {ECO:0000313|EMBL:ECB3379513.1}, EWZ30_22750 GN {ECO:0000313|EMBL:ECD2227786.1}, EZX42_22295 GN {ECO:0000313|EMBL:ECD3428269.1}, FBQ34_21250 GN {ECO:0000313|EMBL:ECB9050082.1}, FDN33_21295 GN {ECO:0000313|EMBL:ECB9159705.1}, FGK47_22155 GN {ECO:0000313|EMBL:ECI8685776.1}, FHJ37_21235 GN {ECO:0000313|EMBL:ECI9072383.1}; OS Salmonella enterica subsp. enterica serovar Reading. OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; OC Enterobacteriaceae; Salmonella. OX NCBI_TaxID=165302 {ECO:0000313|EMBL:ECF3836160.1}; RN [1] {ECO:0000313|EMBL:ECT7930094.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=PNUSAS003387 {ECO:0000313|EMBL:ECT7930094.1}; RG PulseNet: The National Subtyping Network for Foodborne Disease Surveillance; RA Tarr C.L., Trees E., Katz L.S., Carleton-Romer H.A., Stroika S., RA Kucerova Z., Roache K.F., Sabol A.L., Besser J., Gerner-Smidt P.; RL Submitted (JUL-2018) to the EMBL/GenBank/DDBJ databases. RN [2] {ECO:0000313|EMBL:ECS2231151.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=CVM N17S1125 {ECO:0000313|EMBL:ECB8738000.1}, CVM N54259 RC {ECO:0000313|EMBL:ECT4987962.1}, CVM N54280 RC {ECO:0000313|EMBL:ECU5519599.1}, CVM N55400 RC {ECO:0000313|EMBL:ECS2231151.1}, CVM N56234 RC {ECO:0000313|EMBL:ECS2758674.1}, FSIS11813897 RC {ECO:0000313|EMBL:ECT3946125.1}, FSIS11919862 RC {ECO:0000313|EMBL:ECB8847373.1}, and NY86998528 RC {ECO:0000313|EMBL:ECV5544209.1}; RG NARMS: The National Antimicrobial Resistance Monitoring System; RL Submitted (JUL-2018) to the EMBL/GenBank/DDBJ databases. RN [3] {ECO:0000313|EMBL:ECF3836160.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=146074 {ECO:0000313|EMBL:EBS3934698.1}, 186573 RC {ECO:0000313|EMBL:ECG8287559.1}, 261481 RC {ECO:0000313|EMBL:ECD4765434.1}, 275776 {ECO:0000313|EMBL:MLO07687.1, RC ECO:0000313|Proteomes:UP000277002}, 298956 RC {ECO:0000313|EMBL:EBU9895995.1}, 413193 RC {ECO:0000313|EMBL:ECD2227786.1}, 458062 RC {ECO:0000313|EMBL:ECG2745078.1}, 654694 RC {ECO:0000313|EMBL:ECB0315133.1}, 690336 RC {ECO:0000313|EMBL:ECD3428269.1}, and 708215 RC {ECO:0000313|EMBL:ECF3836160.1}; RA Ashton P.M., Dallman T., Nair S., De Pinna E., Peters T., Grant K.; RL Submitted (MAR-2019) to the EMBL/GenBank/DDBJ databases. RN [4] {ECO:0000313|EMBL:ECB9159705.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=CVM-N19771 {ECO:0000313|EMBL:EBW0682999.1}, FSIS11812033 RC {ECO:0000313|EMBL:ECV1784185.1}, FSIS11812504 RC {ECO:0000313|EMBL:ECT2693329.1}, FSIS11920178 RC {ECO:0000313|EMBL:ECB9050082.1}, FSIS21822255 RC {ECO:0000313|EMBL:EBZ2411064.1}, FSIS21924219 RC {ECO:0000313|EMBL:ECB9159705.1}, FSIS31800512 RC {ECO:0000313|EMBL:ECT0134668.1}, FSIS31800555 RC {ECO:0000313|EMBL:ECV3391360.1}, FSIS31800800 RC {ECO:0000313|EMBL:ECV1599414.1}, FSIS31901531 RC {ECO:0000313|EMBL:ECB3379513.1}, FSIS31902023 RC {ECO:0000313|EMBL:ECI8685776.1}, and FSIS31902085 RC {ECO:0000313|EMBL:ECI9072383.1}; RG GenomeTrakr network: Whole genome sequencing for foodborne pathogen traceback; RL Submitted (MAY-2019) to the EMBL/GenBank/DDBJ databases. CC -!- CATALYTIC ACTIVITY: CC Reaction=H2O + NAD(+) = AMP + beta-nicotinamide D-ribonucleotide + 2 CC H(+); Xref=Rhea:RHEA:11800, ChEBI:CHEBI:14649, ChEBI:CHEBI:15377, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57540, ChEBI:CHEBI:456215; CC EC=3.6.1.22; Evidence={ECO:0000256|ARBA:ARBA00001825, CC ECO:0000256|HAMAP-Rule:MF_00297}; CC -!- CATALYTIC ACTIVITY: CC Reaction=H2O + NADH = AMP + 2 H(+) + reduced beta-nicotinamide D- CC ribonucleotide; Xref=Rhea:RHEA:48868, ChEBI:CHEBI:15377, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57945, ChEBI:CHEBI:90832, CC ChEBI:CHEBI:456215; EC=3.6.1.22; CC Evidence={ECO:0000256|ARBA:ARBA00000970, ECO:0000256|HAMAP- CC Rule:MF_00297}; CC -!- COFACTOR: CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420; CC Evidence={ECO:0000256|HAMAP-Rule:MF_00297}; CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035; CC Evidence={ECO:0000256|HAMAP-Rule:MF_00297}; CC Note=Divalent metal cations. Mg(2+) or Mn(2+). {ECO:0000256|HAMAP- CC Rule:MF_00297}; CC -!- COFACTOR: CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035; CC Evidence={ECO:0000256|ARBA:ARBA00001936}; CC -!- COFACTOR: CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105; CC Evidence={ECO:0000256|HAMAP-Rule:MF_00297}; CC Note=Binds 1 zinc ion per subunit. {ECO:0000256|HAMAP-Rule:MF_00297}; CC -!- SUBUNIT: Homodimer. {ECO:0000256|ARBA:ARBA00011738, ECO:0000256|HAMAP- CC Rule:MF_00297}. CC -!- SIMILARITY: Belongs to the Nudix hydrolase family. NudC subfamily. CC {ECO:0000256|ARBA:ARBA00009595, ECO:0000256|HAMAP-Rule:MF_00297}. CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ CC whole genome shotgun (WGS) entry which is preliminary data. CC {ECO:0000313|EMBL:ECF3836160.1}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AAGVGK010000039; EBS3934698.1; -; Genomic_DNA. DR EMBL; AAHDUY010000030; EBU9895995.1; -; Genomic_DNA. DR EMBL; AAHHFV010000021; EBW0682999.1; -; Genomic_DNA. DR EMBL; AAHQMR010000030; EBZ2411064.1; -; Genomic_DNA. DR EMBL; AAHWGP010000029; ECB0315133.1; -; Genomic_DNA. DR EMBL; AAHXHM010000054; ECB3379513.1; -; Genomic_DNA. DR EMBL; AAHYZR010000030; ECB8738000.1; -; Genomic_DNA. DR EMBL; AAHYZU010000033; ECB8847373.1; -; Genomic_DNA. DR EMBL; AAHZBR010000041; ECB9050082.1; -; Genomic_DNA. DR EMBL; AAHZCO010000065; ECB9159705.1; -; Genomic_DNA. DR EMBL; AAIEAE010000029; ECD2227786.1; -; Genomic_DNA. DR EMBL; AAIEKD010000027; ECD3428269.1; -; Genomic_DNA. DR EMBL; AAIEVF010000027; ECD4765434.1; -; Genomic_DNA. DR EMBL; AAIKZN010000031; ECF3836160.1; -; Genomic_DNA. DR EMBL; AAINVR010000034; ECG2745078.1; -; Genomic_DNA. DR EMBL; AAIOXY010000024; ECG8287559.1; -; Genomic_DNA. DR EMBL; AAIWNN010000015; ECI8685776.1; -; Genomic_DNA. DR EMBL; AAIWQF010000064; ECI9072383.1; -; Genomic_DNA. DR EMBL; AAKIXE010000028; ECS2231151.1; -; Genomic_DNA. DR EMBL; AAKJCG010000035; ECS2758674.1; -; Genomic_DNA. DR EMBL; AAKLKV010000039; ECT0134668.1; -; Genomic_DNA. DR EMBL; AAKMFT010000094; ECT2693329.1; -; Genomic_DNA. DR EMBL; AAKMPZ010000024; ECT3946125.1; -; Genomic_DNA. DR EMBL; AAKMZB010000028; ECT4987962.1; -; Genomic_DNA. DR EMBL; AAKNWZ010000014; ECT7930094.1; -; Genomic_DNA. DR EMBL; AAKQHM010000020; ECU5519599.1; -; Genomic_DNA. DR EMBL; AAKSPP010000061; ECV1599414.1; -; Genomic_DNA. DR EMBL; AAKSMT010000079; ECV1784185.1; -; Genomic_DNA. DR EMBL; AAKSBI010000052; ECV3391360.1; -; Genomic_DNA. DR EMBL; AAKRUX010000041; ECV5544209.1; -; Genomic_DNA. DR EMBL; RVCL01000025; MLO07687.1; -; Genomic_DNA. DR RefSeq; WP_000373956.1; NZ_MZFK01000019.1. DR SMR; A0A5I2WV80; -. DR Proteomes; UP000277002; Unassembled WGS sequence. DR GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule. DR GO; GO:0030145; F:manganese ion binding; IEA:UniProtKB-UniRule. DR GO; GO:0000210; F:NAD+ diphosphatase activity; IEA:UniProtKB-UniRule. DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule. DR HAMAP; MF_00297; Nudix_NudC; 1. DR InterPro; IPR022925; NADH_pyroPase_NudC. DR InterPro; IPR015797; NUDIX_hydrolase-like_dom_sf. DR InterPro; IPR020084; NUDIX_hydrolase_CS. DR InterPro; IPR000086; NUDIX_hydrolase_dom. DR InterPro; IPR015376; Znr_NADH_PPase. DR Pfam; PF00293; NUDIX; 1. DR Pfam; PF09297; zf-NADH-PPase; 1. DR SUPFAM; SSF55811; SSF55811; 2. DR PROSITE; PS51462; NUDIX; 1. DR PROSITE; PS00893; NUDIX_BOX; 1. PE 3: Inferred from homology; KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|HAMAP-Rule:MF_00297, KW ECO:0000313|EMBL:ECF3836160.1}; KW Magnesium {ECO:0000256|ARBA:ARBA00022842, ECO:0000256|HAMAP-Rule:MF_00297}; KW Manganese {ECO:0000256|ARBA:ARBA00023211, ECO:0000256|HAMAP-Rule:MF_00297}; KW Metal-binding {ECO:0000256|ARBA:ARBA00022723, ECO:0000256|HAMAP- KW Rule:MF_00297}; KW NAD {ECO:0000256|ARBA:ARBA00023027, ECO:0000256|HAMAP-Rule:MF_00297}; KW Zinc {ECO:0000256|HAMAP-Rule:MF_00297}. FT DOMAIN 125..248 FT /note="Nudix hydrolase" FT /evidence="ECO:0000259|PROSITE:PS51462" FT MOTIF 159..180 FT /note="Nudix box" FT /evidence="ECO:0000256|HAMAP-Rule:MF_00297" FT METAL 98 FT /note="Zinc" FT /evidence="ECO:0000256|HAMAP-Rule:MF_00297" FT METAL 101 FT /note="Zinc" FT /evidence="ECO:0000256|HAMAP-Rule:MF_00297" FT METAL 116 FT /note="Zinc" FT /evidence="ECO:0000256|HAMAP-Rule:MF_00297" FT METAL 119 FT /note="Zinc" FT /evidence="ECO:0000256|HAMAP-Rule:MF_00297" FT METAL 174 FT /note="Divalent metal cation" FT /evidence="ECO:0000256|HAMAP-Rule:MF_00297" FT METAL 178 FT /note="Divalent metal cation" FT /evidence="ECO:0000256|HAMAP-Rule:MF_00297" SQ SEQUENCE 257 AA; 29593 MW; 05C99052BD43DD3D CRC64; MDRIIEKLES GWWIVSHEQK LWLPYGELPH GLAANFDLVG QRALRIGEWQ GEPVWLVLQH RRHDMGSVRQ VIDQDAGLFQ LAGRGVQLAE FYRSHKFCGY CGHPMHPSKT EWAMLCSHCR ERYYPQIAPC IIVAIRREDS ILLAQHVRHR NGVHTVLAGF VEVGETLEQA VAREVMEESG IKVKNLRYVT SQPWPFPQSL MTAFMAEYDS GDIVIDPKEL LEANWYRYDD LPLLPPPGTV ARRLIEDTVA MCRAEYD //