ID A0A5I2WV80_SALET Unreviewed; 257 AA. AC A0A5I2WV80; DT 11-DEC-2019, integrated into UniProtKB/TrEMBL. DT 11-DEC-2019, sequence version 1. DT 26-FEB-2020, entry version 2. DE RecName: Full=NADH pyrophosphatase {ECO:0000256|HAMAP-Rule:MF_00297, ECO:0000256|SAAS:SAAS00345522}; DE EC=3.6.1.22 {ECO:0000256|HAMAP-Rule:MF_00297, ECO:0000256|SAAS:SAAS00042483}; GN Name=nudC {ECO:0000256|HAMAP-Rule:MF_00297, GN ECO:0000313|EMBL:ECF3836160.1}; GN ORFNames=E0U64_22640 {ECO:0000313|EMBL:ECF0955378.1}, E1821_21895 GN {ECO:0000313|EMBL:ECG8287559.1}, E1B31_21850 GN {ECO:0000313|EMBL:ECD4765434.1}, E2G14_21725 GN {ECO:0000313|EMBL:ECF2941432.1}, E2H02_21740 GN {ECO:0000313|EMBL:ECD5903336.1}, E4A02_22065 GN {ECO:0000313|EMBL:ECF3836160.1}, EWC91_21850 GN {ECO:0000313|EMBL:ECG2745078.1}, EWZ30_22750 GN {ECO:0000313|EMBL:ECD2227786.1}, EZX42_22295 GN {ECO:0000313|EMBL:ECD3428269.1}, FGK47_22155 GN {ECO:0000313|EMBL:ECI8685776.1}, FHJ37_21235 GN {ECO:0000313|EMBL:ECI9072383.1}; OS Salmonella enterica subsp. enterica serovar Reading. OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; OC Enterobacteriaceae; Salmonella. OX NCBI_TaxID=165302 {ECO:0000313|EMBL:ECF3836160.1}; RN [1] {ECO:0000313|EMBL:ECF3836160.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=143518 {ECO:0000313|EMBL:ECF2941432.1}, 153169 RC {ECO:0000313|EMBL:ECD5903336.1}, 186573 RC {ECO:0000313|EMBL:ECG8287559.1}, 261481 RC {ECO:0000313|EMBL:ECD4765434.1}, 302603 RC {ECO:0000313|EMBL:ECF0955378.1}, 413193 RC {ECO:0000313|EMBL:ECD2227786.1}, 458062 RC {ECO:0000313|EMBL:ECG2745078.1}, 690336 RC {ECO:0000313|EMBL:ECD3428269.1}, and 708215 RC {ECO:0000313|EMBL:ECF3836160.1}; RA Ashton P.M., Dallman T., Nair S., De Pinna E., Peters T., Grant K.; RL Submitted (MAR-2019) to the EMBL/GenBank/DDBJ databases. RN [2] {ECO:0000313|EMBL:ECI8685776.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=FSIS31902023 {ECO:0000313|EMBL:ECI8685776.1}, and FSIS31902085 RC {ECO:0000313|EMBL:ECI9072383.1}; RG GenomeTrakr network: Whole genome sequencing for foodborne pathogen traceback; RL Submitted (JUL-2019) to the EMBL/GenBank/DDBJ databases. CC -!- CATALYTIC ACTIVITY: CC Reaction=H2O + NAD(+) = AMP + beta-nicotinamide D-ribonucleotide + 2 CC H(+); Xref=Rhea:RHEA:11800, ChEBI:CHEBI:14649, ChEBI:CHEBI:15377, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57540, ChEBI:CHEBI:456215; CC EC=3.6.1.22; Evidence={ECO:0000256|HAMAP-Rule:MF_00297, CC ECO:0000256|SAAS:SAAS01118353}; CC -!- CATALYTIC ACTIVITY: CC Reaction=H2O + NADH = AMP + 2 H(+) + reduced beta-nicotinamide D- CC ribonucleotide; Xref=Rhea:RHEA:48868, ChEBI:CHEBI:15377, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57945, ChEBI:CHEBI:90832, CC ChEBI:CHEBI:456215; EC=3.6.1.22; Evidence={ECO:0000256|HAMAP- CC Rule:MF_00297, ECO:0000256|SAAS:SAAS01118352}; CC -!- COFACTOR: CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035; CC Evidence={ECO:0000256|SAAS:SAAS00608010}; CC -!- SUBUNIT: Homodimer. {ECO:0000256|HAMAP-Rule:MF_00297, CC ECO:0000256|SAAS:SAAS00042551}. CC -!- SIMILARITY: Belongs to the Nudix hydrolase family. NudC subfamily. CC {ECO:0000256|HAMAP-Rule:MF_00297, ECO:0000256|SAAS:SAAS00541212}. CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ CC whole genome shotgun (WGS) entry which is preliminary data. CC {ECO:0000313|EMBL:ECF3836160.1}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AAIEAE010000029; ECD2227786.1; -; Genomic_DNA. DR EMBL; AAIEKD010000027; ECD3428269.1; -; Genomic_DNA. DR EMBL; AAIEVF010000027; ECD4765434.1; -; Genomic_DNA. DR EMBL; AAIFFZ010000024; ECD5903336.1; -; Genomic_DNA. DR EMBL; AAIKBL010000027; ECF0955378.1; -; Genomic_DNA. DR EMBL; AAIKSC010000050; ECF2941432.1; -; Genomic_DNA. DR EMBL; AAIKZN010000031; ECF3836160.1; -; Genomic_DNA. DR EMBL; AAINVR010000034; ECG2745078.1; -; Genomic_DNA. DR EMBL; AAIOXY010000024; ECG8287559.1; -; Genomic_DNA. DR EMBL; AAIWNN010000015; ECI8685776.1; -; Genomic_DNA. DR EMBL; AAIWQF010000064; ECI9072383.1; -; Genomic_DNA. DR RefSeq; WP_000373956.1; NZ_MZFK01000019.1. DR GO; GO:0000210; F:NAD+ diphosphatase activity; IEA:UniProtKB-EC. DR HAMAP; MF_00297; Nudix_NudC; 1. DR InterPro; IPR022925; NADH_pyroPase_NudC. DR InterPro; IPR015797; NUDIX_hydrolase-like_dom_sf. DR InterPro; IPR020084; NUDIX_hydrolase_CS. DR InterPro; IPR000086; NUDIX_hydrolase_dom. DR InterPro; IPR015376; Znr_NADH_PPase. DR Pfam; PF00293; NUDIX; 1. DR Pfam; PF09297; zf-NADH-PPase; 1. DR SUPFAM; SSF55811; SSF55811; 2. DR PROSITE; PS51462; NUDIX; 1. DR PROSITE; PS00893; NUDIX_BOX; 1. PE 3: Inferred from homology; KW Hydrolase {ECO:0000256|HAMAP-Rule:MF_00297, ECO:0000256|SAAS:SAAS00437314, KW ECO:0000313|EMBL:ECF3836160.1}; KW Magnesium {ECO:0000256|HAMAP-Rule:MF_00297, ECO:0000256|SAAS:SAAS00016165}; KW Manganese {ECO:0000256|HAMAP-Rule:MF_00297, ECO:0000256|SAAS:SAAS00016184}; KW Metal-binding {ECO:0000256|HAMAP-Rule:MF_00297, KW ECO:0000256|SAAS:SAAS00016198}; KW NAD {ECO:0000256|HAMAP-Rule:MF_00297, ECO:0000256|SAAS:SAAS00016191}; KW Zinc {ECO:0000256|HAMAP-Rule:MF_00297, ECO:0000256|SAAS:SAAS01085471}. FT DOMAIN 125..248 FT /note="Nudix hydrolase" FT /evidence="ECO:0000259|PROSITE:PS51462" FT MOTIF 159..180 FT /note="Nudix box" FT /evidence="ECO:0000256|HAMAP-Rule:MF_00297" FT METAL 98 FT /note="Zinc" FT /evidence="ECO:0000256|HAMAP-Rule:MF_00297" FT METAL 101 FT /note="Zinc" FT /evidence="ECO:0000256|HAMAP-Rule:MF_00297" FT METAL 116 FT /note="Zinc" FT /evidence="ECO:0000256|HAMAP-Rule:MF_00297" FT METAL 119 FT /note="Zinc" FT /evidence="ECO:0000256|HAMAP-Rule:MF_00297" FT METAL 174 FT /note="Divalent metal cation" FT /evidence="ECO:0000256|HAMAP-Rule:MF_00297" FT METAL 178 FT /note="Divalent metal cation" FT /evidence="ECO:0000256|HAMAP-Rule:MF_00297" SQ SEQUENCE 257 AA; 29593 MW; 05C99052BD43DD3D CRC64; MDRIIEKLES GWWIVSHEQK LWLPYGELPH GLAANFDLVG QRALRIGEWQ GEPVWLVLQH RRHDMGSVRQ VIDQDAGLFQ LAGRGVQLAE FYRSHKFCGY CGHPMHPSKT EWAMLCSHCR ERYYPQIAPC IIVAIRREDS ILLAQHVRHR NGVHTVLAGF VEVGETLEQA VAREVMEESG IKVKNLRYVT SQPWPFPQSL MTAFMAEYDS GDIVIDPKEL LEANWYRYDD LPLLPPPGTV ARRLIEDTVA MCRAEYD //