ID A0A5A7RCS8_STRAF Unreviewed; 267 AA. AC A0A5A7RCS8; DT 13-NOV-2019, integrated into UniProtKB/TrEMBL. DT 13-NOV-2019, sequence version 1. DT 08-NOV-2023, entry version 17. DE SubName: Full=2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein {ECO:0000313|EMBL:GER55543.1}; GN ORFNames=STAS_33219 {ECO:0000313|EMBL:GER55543.1}; OS Striga asiatica (Asiatic witchweed) (Buchnera asiatica). OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC asterids; lamiids; Lamiales; Orobanchaceae; Buchnereae; Striga. OX NCBI_TaxID=4170 {ECO:0000313|EMBL:GER55543.1, ECO:0000313|Proteomes:UP000325081}; RN [1] {ECO:0000313|Proteomes:UP000325081} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=cv. UVA1 {ECO:0000313|Proteomes:UP000325081}; RX PubMed=31522940; DOI=10.1016/j.cub.2019.07.086; RA Yoshida S., Kim S., Wafula E.K., Tanskanen J., Kim Y.M., Honaas L., RA Yang Z., Spallek T., Conn C.E., Ichihashi Y., Cheong K., Cui S., Der J.P., RA Gundlach H., Jiao Y., Hori C., Ishida J.K., Kasahara H., Kiba T., Kim M.S., RA Koo N., Laohavisit A., Lee Y.H., Lumba S., McCourt P., Mortimer J.C., RA Mutuku J.M., Nomura T., Sasaki-Sekimoto Y., Seto Y., Wang Y., Wakatake T., RA Sakakibara H., Demura T., Yamaguchi S., Yoneyama K., Manabe R.I., RA Nelson D.C., Schulman A.H., Timko M.P., dePamphilis C.W., Choi D., RA Shirasu K.; RT "Genome Sequence of Striga asiatica Provides Insight into the Evolution of RT Plant Parasitism."; RL Curr. Biol. 29:3041-3052.e4(2019). CC -!- CATALYTIC ACTIVITY: CC Reaction=2-oxoglutarate + L-prolyl-[collagen] + O2 = CO2 + succinate + CC trans-4-hydroxy-L-prolyl-[collagen]; Xref=Rhea:RHEA:18945, Rhea:RHEA- CC COMP:11676, Rhea:RHEA-COMP:11680, ChEBI:CHEBI:15379, CC ChEBI:CHEBI:16526, ChEBI:CHEBI:16810, ChEBI:CHEBI:30031, CC ChEBI:CHEBI:50342, ChEBI:CHEBI:61965; EC=1.14.11.2; CC Evidence={ECO:0000256|ARBA:ARBA00024151}; CC -!- COFACTOR: CC Name=L-ascorbate; Xref=ChEBI:CHEBI:38290; CC Evidence={ECO:0000256|ARBA:ARBA00001961}; CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane CC {ECO:0000256|ARBA:ARBA00004648}; Single-pass type II membrane protein CC {ECO:0000256|ARBA:ARBA00004648}. Membrane CC {ECO:0000256|ARBA:ARBA00004606}; Single-pass type II membrane protein CC {ECO:0000256|ARBA:ARBA00004606}. CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ CC whole genome shotgun (WGS) entry which is preliminary data. CC {ECO:0000313|EMBL:GER55543.1}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; BKCP01011959; GER55543.1; -; Genomic_DNA. DR AlphaFoldDB; A0A5A7RCS8; -. DR Proteomes; UP000325081; Unassembled WGS sequence. DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell. DR GO; GO:0051213; F:dioxygenase activity; IEA:UniProtKB-KW. DR GO; GO:0005506; F:iron ion binding; IEA:InterPro. DR GO; GO:0031418; F:L-ascorbic acid binding; IEA:InterPro. DR GO; GO:0016705; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; IEA:InterPro. DR Gene3D; 2.60.120.620; q2cbj1_9rhob like domain; 1. DR InterPro; IPR005123; Oxoglu/Fe-dep_dioxygenase. DR InterPro; IPR045054; P4HA-like. DR InterPro; IPR006620; Pro_4_hyd_alph. DR InterPro; IPR044862; Pro_4_hyd_alph_FE2OG_OXY. DR PANTHER; PTHR10869:SF42; PROLYL 4-HYDROXYLASE 1; 1. DR PANTHER; PTHR10869; PROLYL 4-HYDROXYLASE ALPHA SUBUNIT; 1. DR Pfam; PF13640; 2OG-FeII_Oxy_3; 1. DR SMART; SM00702; P4Hc; 1. DR PROSITE; PS51471; FE2OG_OXY; 1. PE 4: Predicted; KW Dioxygenase {ECO:0000256|ARBA:ARBA00022964}; KW Iron {ECO:0000256|ARBA:ARBA00023004}; Membrane {ECO:0000256|SAM:Phobius}; KW Metal-binding {ECO:0000256|ARBA:ARBA00022723}; KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002}; KW Reference proteome {ECO:0000313|Proteomes:UP000325081}; KW Signal-anchor {ECO:0000256|ARBA:ARBA00022968}; KW Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius}; KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989, KW ECO:0000256|SAM:Phobius}. FT TRANSMEM 6..31 FT /note="Helical" FT /evidence="ECO:0000256|SAM:Phobius" FT DOMAIN 146..263 FT /note="Fe2OG dioxygenase" FT /evidence="ECO:0000259|PROSITE:PS51471" SQ SEQUENCE 267 AA; 30007 MW; 1D76C20ACED75F9A CRC64; MAASWMRFFL ILLIILTVGM MIGVLLQLAF IRRLEDSYGA KSSLSRAQSL NADVSYWGDK EAAALRLGYV KPEVISWSPR IIVFHNFLSP EECDYLRAIA KPRLQGMKSK VRTSSGMFLT VEERKYPMIE TIEKRISVYS QVPVENGERI QKHRYEKGEL YRPHHDYFSD TFNLKHGGQR VATMLMYLSD NVEGGETYFP QAGTGECSCG GEKVPGLCVK PVKGDAVLFW SMGLDGKSDP NSLHGGCAVL SGEKWSATKW MRQSITS //