ID A0A520PM79_9PROT Unreviewed; 245 AA. AC A0A520PM79; DT 16-OCT-2019, integrated into UniProtKB/TrEMBL. DT 16-OCT-2019, sequence version 1. DT 13-NOV-2019, entry version 2. DE RecName: Full=Ribosomal RNA small subunit methyltransferase E {ECO:0000256|PIRNR:PIRNR015601}; DE EC=2.1.1.193 {ECO:0000256|PIRNR:PIRNR015601}; GN ORFNames=EVA80_02400 {ECO:0000313|EMBL:RZO46831.1}; OS Proteobacteria bacterium. OC Bacteria; Proteobacteria. OX NCBI_TaxID=1977087 {ECO:0000313|EMBL:RZO46831.1}; RN [1] {ECO:0000313|EMBL:RZO46831.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=MED-G180 {ECO:0000313|EMBL:RZO46831.1}; RA Haro-Moreno J.M., Rodriguez-Valera F., Lopez-Perez M.; RT "Prokaryotic population dynamics and viral predation in marine RT succession experiment using metagenomics: the confinement effect."; RL Submitted (FEB-2019) to the EMBL/GenBank/DDBJ databases. CC -!- FUNCTION: Specifically methylates the N3 position of the uracil CC ring of uridine 1498 (m3U1498) in 16S rRNA. Acts on the fully CC assembled 30S ribosomal subunit. {ECO:0000256|PIRNR:PIRNR015601}. CC -!- CATALYTIC ACTIVITY: CC Reaction=S-adenosyl-L-methionine + uridine(1498) in 16S rRNA = CC H(+) + N(3)-methyluridine(1498) in 16S rRNA + S-adenosyl-L- CC homocysteine; Xref=Rhea:RHEA:42920, Rhea:RHEA-COMP:10283, CC Rhea:RHEA-COMP:10284, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, CC ChEBI:CHEBI:59789, ChEBI:CHEBI:65315, ChEBI:CHEBI:74502; CC EC=2.1.1.193; Evidence={ECO:0000256|PIRNR:PIRNR015601}; CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|PIRNR:PIRNR015601}. CC -!- SIMILARITY: Belongs to the RNA methyltransferase RsmE family. CC {ECO:0000256|PIRNR:PIRNR015601}. CC -!- CAUTION: The sequence shown here is derived from an CC EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is CC preliminary data. {ECO:0000313|EMBL:RZO46831.1}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; SHAS01000004; RZO46831.1; -; Genomic_DNA. DR Gene3D; 3.40.1280.10; -; 1. DR InterPro; IPR029028; Alpha/beta_knot_MTases. DR InterPro; IPR006700; rRNA_ssu_MeTrfase-E. DR InterPro; IPR029026; tRNA_m1G_MTases_N. DR PANTHER; PTHR30027; PTHR30027; 1. DR Pfam; PF04452; Methyltrans_RNA; 1. DR PIRSF; PIRSF015601; MTase_slr0722; 1. DR SUPFAM; SSF75217; SSF75217; 1. DR TIGRFAMs; TIGR00046; TIGR00046; 1. PE 3: Inferred from homology; KW Cytoplasm {ECO:0000256|PIRNR:PIRNR015601}; KW Methyltransferase {ECO:0000256|PIRNR:PIRNR015601, KW ECO:0000313|EMBL:RZO46831.1}; KW rRNA processing {ECO:0000256|PIRNR:PIRNR015601}; KW S-adenosyl-L-methionine {ECO:0000256|PIRNR:PIRNR015601}; KW Transferase {ECO:0000256|PIRNR:PIRNR015601, KW ECO:0000313|EMBL:RZO46831.1}. SQ SEQUENCE 245 AA; 28118 MW; 8DB886E7CAE6C80F CRC64; MSAYFKFEGE LRLALRAEMQ GEEVRHISQS RRLRPGDDFL LQDRLGQRFR VKLLNQIRQR LEFEVLEAVV VPEPSPLLLE LWLALPKEKA LELILQKGVE LGVSRVVLFQ GEFSSGRDET PTINTQQRWE RIMSEACKQS GRQFSPTWKW FQSLEAALGQ TTATDQHWIF APLLDSSEWP ISREEMVGQK HTLWIGPEGG WHASELALAH QAKARSLTLG PRILRTETAA ISAMTIFQHR FGDMN //