ID A0A520PM79_UNCPS Unreviewed; 245 AA. AC A0A520PM79; DT 16-OCT-2019, integrated into UniProtKB/TrEMBL. DT 16-OCT-2019, sequence version 1. DT 13-SEP-2023, entry version 12. DE RecName: Full=Ribosomal RNA small subunit methyltransferase E {ECO:0000256|PIRNR:PIRNR015601}; DE EC=2.1.1.193 {ECO:0000256|PIRNR:PIRNR015601}; GN ORFNames=EVA80_02400 {ECO:0000313|EMBL:RZO46831.1}; OS Pseudomonadota bacterium. OC Bacteria; Pseudomonadota. OX NCBI_TaxID=1977087 {ECO:0000313|EMBL:RZO46831.1, ECO:0000313|Proteomes:UP000316779}; RN [1] {ECO:0000313|EMBL:RZO46831.1, ECO:0000313|Proteomes:UP000316779} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=MED-G180 {ECO:0000313|EMBL:RZO46831.1}; RA Haro-Moreno J.M., Rodriguez-Valera F., Lopez-Perez M.; RT "Prokaryotic population dynamics and viral predation in marine succession RT experiment using metagenomics: the confinement effect."; RL Submitted (FEB-2019) to the EMBL/GenBank/DDBJ databases. CC -!- FUNCTION: Specifically methylates the N3 position of the uracil ring of CC uridine 1498 (m3U1498) in 16S rRNA. Acts on the fully assembled 30S CC ribosomal subunit. {ECO:0000256|ARBA:ARBA00025699, CC ECO:0000256|PIRNR:PIRNR015601}. CC -!- CATALYTIC ACTIVITY: CC Reaction=S-adenosyl-L-methionine + uridine(1498) in 16S rRNA = H(+) + CC N(3)-methyluridine(1498) in 16S rRNA + S-adenosyl-L-homocysteine; CC Xref=Rhea:RHEA:42920, Rhea:RHEA-COMP:10283, Rhea:RHEA-COMP:10284, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, CC ChEBI:CHEBI:65315, ChEBI:CHEBI:74502; EC=2.1.1.193; CC Evidence={ECO:0000256|ARBA:ARBA00000792, CC ECO:0000256|PIRNR:PIRNR015601}; CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496, CC ECO:0000256|PIRNR:PIRNR015601}. CC -!- SIMILARITY: Belongs to the RNA methyltransferase RsmE family. CC {ECO:0000256|ARBA:ARBA00005528, ECO:0000256|PIRNR:PIRNR015601}. CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ CC whole genome shotgun (WGS) entry which is preliminary data. CC {ECO:0000313|EMBL:RZO46831.1}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; SHAS01000004; RZO46831.1; -; Genomic_DNA. DR AlphaFoldDB; A0A520PM79; -. DR Proteomes; UP000316779; Unassembled WGS sequence. DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell. DR GO; GO:0008168; F:methyltransferase activity; IEA:UniProtKB-KW. DR GO; GO:0032259; P:methylation; IEA:UniProtKB-KW. DR GO; GO:0006364; P:rRNA processing; IEA:UniProtKB-KW. DR CDD; cd18084; RsmE-like; 1. DR Gene3D; 3.40.1280.10; -; 1. DR InterPro; IPR029028; Alpha/beta_knot_MTases. DR InterPro; IPR006700; RsmE. DR InterPro; IPR046886; RsmE_MTase_dom. DR InterPro; IPR029026; tRNA_m1G_MTases_N. DR NCBIfam; TIGR00046; RsmE family RNA methyltransferase; 1. DR PANTHER; PTHR30027:SF3; 16S RRNA (URACIL(1498)-N(3))-METHYLTRANSFERASE; 1. DR PANTHER; PTHR30027; RIBOSOMAL RNA SMALL SUBUNIT METHYLTRANSFERASE E; 1. DR Pfam; PF04452; Methyltrans_RNA; 1. DR PIRSF; PIRSF015601; MTase_slr0722; 1. DR SUPFAM; SSF75217; alpha/beta knot; 1. PE 3: Inferred from homology; KW Cytoplasm {ECO:0000256|PIRNR:PIRNR015601}; KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603, KW ECO:0000256|PIRNR:PIRNR015601}; KW rRNA processing {ECO:0000256|PIRNR:PIRNR015601}; KW S-adenosyl-L-methionine {ECO:0000256|PIRNR:PIRNR015601}; KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|PIRNR:PIRNR015601}. FT DOMAIN 74..238 FT /note="Ribosomal RNA small subunit methyltransferase E FT methyltransferase" FT /evidence="ECO:0000259|Pfam:PF04452" SQ SEQUENCE 245 AA; 28118 MW; 8DB886E7CAE6C80F CRC64; MSAYFKFEGE LRLALRAEMQ GEEVRHISQS RRLRPGDDFL LQDRLGQRFR VKLLNQIRQR LEFEVLEAVV VPEPSPLLLE LWLALPKEKA LELILQKGVE LGVSRVVLFQ GEFSSGRDET PTINTQQRWE RIMSEACKQS GRQFSPTWKW FQSLEAALGQ TTATDQHWIF APLLDSSEWP ISREEMVGQK HTLWIGPEGG WHASELALAH QAKARSLTLG PRILRTETAA ISAMTIFQHR FGDMN //