ID A0A513WY84_PRRSV Unreviewed; 1460 AA. AC A0A513WY84; DT 16-OCT-2019, integrated into UniProtKB/TrEMBL. DT 16-OCT-2019, sequence version 1. DT 07-APR-2021, entry version 9. DE RecName: Full=Non-structural protein 8 {ECO:0000256|ARBA:ARBA00016245}; DE EC=3.6.4.13 {ECO:0000256|ARBA:ARBA00012552}; DE AltName: Full=ORF1ab polyprotein {ECO:0000256|ARBA:ARBA00015878}; DE AltName: Full=RNA-directed RNA polymerase {ECO:0000256|ARBA:ARBA00022412}; DE AltName: Full=Replicase polyprotein 1ab {ECO:0000256|ARBA:ARBA00022087}; DE Flags: Fragment; OS Porcine reproductive and respiratory syndrome virus (PRRSV). OC Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; OC Nidovirales; Arnidovirineae; Arteriviridae; unclassified Arteriviridae. OX NCBI_TaxID=28344 {ECO:0000313|EMBL:QDH06638.1}; OH NCBI_TaxID=9823; Sus scrofa (Pig). RN [1] {ECO:0000313|EMBL:QDH06638.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=180900-5 {ECO:0000313|EMBL:QDH06638.1}; RA Chang Y., Li T., Wang J., Jin C., Tan F., Li X., Tian K.; RL Submitted (DEC-2018) to the EMBL/GenBank/DDBJ databases. CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000256|ARBA:ARBA00004141}; Multi- CC pass membrane protein {ECO:0000256|ARBA:ARBA00004141}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; MK303390; QDH06638.1; -; Genomic_RNA. DR GO; GO:0033644; C:host cell membrane; IEA:UniProtKB-KW. DR GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell. DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW. DR GO; GO:0003723; F:RNA binding; IEA:InterPro. DR GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC. DR GO; GO:0003968; F:RNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-KW. DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro. DR GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro. DR GO; GO:0039694; P:viral RNA genome replication; IEA:InterPro. DR CDD; cd21410; 1B_av_Nsp10-like; 1. DR CDD; cd21160; NendoU_av_Nsp11-like; 1. DR CDD; cd21166; NTD_av_Nsp11-like; 1. DR CDD; cd21405; ZBD_av_Nsp10-like; 1. DR InterPro; IPR027351; (+)RNA_virus_helicase_core_dom. DR InterPro; IPR043502; DNA/RNA_pol_sf. DR InterPro; IPR037227; EndoU-like. DR InterPro; IPR043609; NendoU_nidovirus. DR InterPro; IPR044348; NSP10_1B_Av. DR InterPro; IPR027355; NSP10_Av_ZBD. DR InterPro; IPR044320; NSP11_Av_N. DR InterPro; IPR044314; NSP11_NendoU_Av. DR InterPro; IPR027417; P-loop_NTPase. DR InterPro; IPR001205; RNA-dir_pol_C. DR InterPro; IPR007094; RNA-dir_pol_PSvirus. DR Pfam; PF19215; CoV_NSP15_C; 1. DR Pfam; PF00680; RdRP_1; 1. DR Pfam; PF01443; Viral_helicase1; 1. DR SUPFAM; SSF142877; SSF142877; 1. DR SUPFAM; SSF52540; SSF52540; 2. DR SUPFAM; SSF56672; SSF56672; 1. DR PROSITE; PS51652; AV_ZBD; 1. DR PROSITE; PS51657; PSRV_HELICASE; 1. DR PROSITE; PS50507; RDRP_SSRNA_POS; 1. PE 4: Predicted; KW ATP-binding {ECO:0000256|ARBA:ARBA00022840}; KW Host membrane {ECO:0000256|ARBA:ARBA00022870}; KW Hydrolase {ECO:0000256|ARBA:ARBA00022801}; KW Membrane {ECO:0000256|ARBA:ARBA00023136}; KW Metal-binding {ECO:0000256|ARBA:ARBA00022771, ECO:0000256|PROSITE- KW ProRule:PRU00985}; Nucleotide-binding {ECO:0000256|ARBA:ARBA00022840}; KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695}; KW Ribosomal frameshifting {ECO:0000256|ARBA:ARBA00022758}; KW RNA-directed RNA polymerase {ECO:0000256|ARBA:ARBA00022484}; KW Transferase {ECO:0000256|ARBA:ARBA00022679}; KW Viral RNA replication {ECO:0000256|ARBA:ARBA00022953}; KW Zinc {ECO:0000256|ARBA:ARBA00022833}; KW Zinc-finger {ECO:0000256|ARBA:ARBA00022771, ECO:0000256|PROSITE- KW ProRule:PRU00985}. FT DOMAIN 391..525 FT /note="RdRp catalytic" FT /evidence="ECO:0000259|PROSITE:PS50507" FT DOMAIN 646..709 FT /note="AV ZBD" FT /evidence="ECO:0000259|PROSITE:PS51652" FT DOMAIN 759..1048 FT /note="(+)RNA virus helicase C-terminal" FT /evidence="ECO:0000259|PROSITE:PS51657" FT NON_TER 1 FT /evidence="ECO:0000313|EMBL:QDH06638.1" SQ SEQUENCE 1460 AA; 161201 MW; ECD39982308CE534 CRC64; TGFKLLAASG LTRCGRGGLV VTETAVKIVK YHSRTFTLGP LDLKVTSETE VKKSTEQGHA VVANLCSGVV LMRPHPPSLV DVLLVPGLDT IPGIQPGHGA GNMGVNGAIW DFETAPTKAE LELSKQIIQA CEIRRGDAPN LQLPYKLYPV RGDPERRNGR LSNTRFGDLP YKTPQDTKSA IHAACCLHPN GAPVSDGKST LGTTLQRGFE LYVPTVPYSV LEYLDSRPDT PVMCTKHGTS EAAAEDLQKY NLSTQGFVLP GVLRLVRKFV FGHIGKAPPL YLPSTYPAKN SMAGINGQRF PTKDVQSIPE IDEMCARAVK ENWQTVTPCT LKKQYCSKLK TRTILGTNNF IALAHRSALS GVTQAFMKKA WKSPIALGKN KFKELHCTVA GRCLEADLAS CDRSTPAIVR WFTANLLYEL AGCEEYLPSY VLNCCHDLVA TQDGAFTKRG GLSSGDPVTS VSNTVYSLVI YAQHMVLSAL KMGHEIGLKF LEEQLKFEDL IEIQPMLVYS DDLVLYAEKP TFPNYHWWVE HLDLMLGFKT DPKKTVITDK PSFLGCRIEA GRQLVPNRDR ILAALAYHMK AQNASEYYAS AAAILMDSCA CIDYDPEWYE DLICGIAQCA RQDGYSFPGP AFFMSMWEKL KGHNEGKKFR HCGICDAKAD HASACGLDLC LFHSHFHQHC PVTLSCGHHA GSKECPQCQS PVGAGKSPLD TVLQQVPYKP PRTVIMKVNN KTTALDPGRY QSRRGLVAVK RGIAGNEVDL ADGDYQVVPL LPTCKDINMV KVACNVLLSK FIVGPPGSGK TTWLLSQVQN DDVIYTPTHQ TMFDIVSALK VCRYSIPVAS GLPFPPPSRS GPWVRLVASG HTPGRVSYLD EAGYCNHLDI LRLLSKTPLV CLGDLQQLHP VGFDSCCYVF NQMPHKQLTT IYRFGPNICA AIQPCYREKL ESKARNTRVV FTTRPVAYGQ VLTPFHKDRD GSAITIDSSQ GATFDVVTLH LPSPKSLNKP RALVAITRAR HGLFIYDPHN QLQEFFNLTP EHTDCNLVFH RGDELVVLDP DNVTTTVAKA LEAGPSQFRV TDPRCKSLLA ACSVSLEGSC MPLPQVAHNL GFYFSPDSPA FAPLPKELAP HWPVVTHQNN HAWPDRLVAS MRPIDARYSK PMVGAGYVVG PSTFLGTPGV VSYYLTLYIR GEPQALPETL VSTGRIATDC REYLDTAEEE TAKELPHAFI GDVKGTTVGG CHHITSKYLP RFLPKDSVAV VGVSSPGKAA KAVCTLTDVY LPDLRPYLQP ETASKCWKLK LDFRDVRLMV WKGATAYFQL EGLTWSALPD YARFIQLPKD AVVYIDPCIG PAAANRKVVR TTDWRADLAV TPYDYGAQTI LTTAWFEDLG PQWKILGLQP FKRALGLENT EDWAILARRM NDGKDYTDYN WTCVRERPHA IYGRARDHTY HFALGTELQV ELGKPRPLSE //