ID A0A4Y3W455_GLUNI Unreviewed; 324 AA. AC A0A4Y3W455; DT 18-SEP-2019, integrated into UniProtKB/TrEMBL. DT 18-SEP-2019, sequence version 1. DT 11-DEC-2019, entry version 4. DE RecName: Full=Porphobilinogen deaminase {ECO:0000256|HAMAP-Rule:MF_00260}; DE Short=PBG {ECO:0000256|HAMAP-Rule:MF_00260}; DE EC=2.5.1.61 {ECO:0000256|HAMAP-Rule:MF_00260}; DE AltName: Full=Hydroxymethylbilane synthase {ECO:0000256|HAMAP-Rule:MF_00260}; DE Short=HMBS {ECO:0000256|HAMAP-Rule:MF_00260}; DE AltName: Full=Pre-uroporphyrinogen synthase {ECO:0000256|HAMAP-Rule:MF_00260}; GN Name=hemC1 {ECO:0000313|EMBL:GEC12026.1}; GN Synonyms=hemC {ECO:0000256|HAMAP-Rule:MF_00260}; GN ORFNames=ANI01nite_12290 {ECO:0000313|EMBL:GEC12026.1}; OS Glutamicibacter nicotianae (Arthrobacter nicotianae). OC Bacteria; Actinobacteria; Micrococcales; Micrococcaceae; Glutamicibacter. OX NCBI_TaxID=37929 {ECO:0000313|EMBL:GEC12026.1, ECO:0000313|Proteomes:UP000316242}; RN [1] {ECO:0000313|EMBL:GEC12026.1, ECO:0000313|Proteomes:UP000316242} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=NBRC 14234 {ECO:0000313|EMBL:GEC12026.1, RC ECO:0000313|Proteomes:UP000316242}; RA Hosoyama A., Uohara A., Ohji S., Ichikawa N.; RT "Whole genome shotgun sequence of Glutamicibacter nicotianae NBRC 14234."; RL Submitted (JUN-2019) to the EMBL/GenBank/DDBJ databases. CC -!- FUNCTION: Tetrapolymerization of the monopyrrole PBG into the CC hydroxymethylbilane pre-uroporphyrinogen in several discrete steps. CC {ECO:0000256|HAMAP-Rule:MF_00260, ECO:0000256|SAAS:SAAS00129929}. CC -!- CATALYTIC ACTIVITY: CC Reaction=H2O + 4 porphobilinogen = hydroxymethylbilane + 4 NH4(+); CC Xref=Rhea:RHEA:13185, ChEBI:CHEBI:15377, ChEBI:CHEBI:28938, CC ChEBI:CHEBI:57845, ChEBI:CHEBI:58126; EC=2.5.1.61; CC Evidence={ECO:0000256|HAMAP-Rule:MF_00260, CC ECO:0000256|SAAS:SAAS01118549}; CC -!- COFACTOR: CC Name=dipyrromethane; Xref=ChEBI:CHEBI:60342; CC Evidence={ECO:0000256|HAMAP-Rule:MF_00260}; CC Note=Binds 1 dipyrromethane group covalently. {ECO:0000256|HAMAP- CC Rule:MF_00260}; CC -!- PATHWAY: Porphyrin-containing compound metabolism; protoporphyrin-IX CC biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 2/4. CC {ECO:0000256|SAAS:SAAS00018329}. CC -!- SUBUNIT: Monomer. {ECO:0000256|HAMAP-Rule:MF_00260}. CC -!- MISCELLANEOUS: The porphobilinogen subunits are added to the CC dipyrromethane group. {ECO:0000256|HAMAP-Rule:MF_00260}. CC -!- SIMILARITY: Belongs to the HMBS family. {ECO:0000256|HAMAP- CC Rule:MF_00260, ECO:0000256|SAAS:SAAS00542451}. CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ CC whole genome shotgun (WGS) entry which is preliminary data. CC {ECO:0000313|EMBL:GEC12026.1}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; BJNE01000003; GEC12026.1; -; Genomic_DNA. DR UniPathway; UPA00251; UER00319. DR Proteomes; UP000316242; Unassembled WGS sequence. DR GO; GO:0004418; F:hydroxymethylbilane synthase activity; IEA:UniProtKB-UniRule. DR GO; GO:0018160; P:peptidyl-pyrromethane cofactor linkage; IEA:InterPro. DR GO; GO:0006782; P:protoporphyrinogen IX biosynthetic process; IEA:UniProtKB-UniPathway. DR Gene3D; 3.30.160.40; -; 1. DR HAMAP; MF_00260; Porphobil_deam; 1. DR InterPro; IPR000860; HemC. DR InterPro; IPR022419; Porphobilin_deaminase_cofac_BS. DR InterPro; IPR022417; Porphobilin_deaminase_N. DR InterPro; IPR022418; Porphobilinogen_deaminase_C. DR InterPro; IPR036803; Porphobilinogen_deaminase_C_sf. DR PANTHER; PTHR11557; PTHR11557; 1. DR Pfam; PF01379; Porphobil_deam; 1. DR Pfam; PF03900; Porphobil_deamC; 1. DR PIRSF; PIRSF001438; 4pyrrol_synth_OHMeBilane_synth; 1. DR PRINTS; PR00151; PORPHBDMNASE. DR SUPFAM; SSF54782; SSF54782; 1. DR TIGRFAMs; TIGR00212; hemC; 1. DR PROSITE; PS00533; PORPHOBILINOGEN_DEAM; 1. PE 3: Inferred from homology; KW Porphyrin biosynthesis {ECO:0000256|HAMAP-Rule:MF_00260, KW ECO:0000256|SAAS:SAAS00425415}; KW Transferase {ECO:0000256|HAMAP-Rule:MF_00260, KW ECO:0000256|SAAS:SAAS00425344}. FT DOMAIN 6..220 FT /note="Porphobil_deam" FT /evidence="ECO:0000259|Pfam:PF01379" FT DOMAIN 242..313 FT /note="Porphobil_deamC" FT /evidence="ECO:0000259|Pfam:PF03900" FT MOD_RES 257 FT /note="S-(dipyrrolylmethanemethyl)cysteine" FT /evidence="ECO:0000256|HAMAP-Rule:MF_00260" SQ SEQUENCE 324 AA; 33368 MW; 03D768B786076572 CRC64; MSEAFRVGTR GSALATTQTG WAAQQLAEIS GLEVQTVLVK TEGDVVTGSL ATLGGTGVFA AALRQHLLDS GCDLAVHSLK DLPTAQPEGL VLAAIPPRED VRDALCSRDS LGLDQLPQGA KIGTGSPRRA AQLLAYRSDL QIVDIRGNVP TRLGRVKGVG ETLDDGSVGR STQGDLDAVI LAAAGMRRLG LEKHITEYLD PAIMLPAPGQ GALALETRTA GSGHKSLDAA LAQFDDAATR MQVEAERALL AELEAGCAAP IGALAHIEGD ELILEAVACN PDGSQTMRRS ARTEASSVQS AQQLGRSLAQ SMIADGAAVV AGLA //