ID A0A4U0YMJ6_9GAMM Unreviewed; 352 AA. AC A0A4U0YMJ6; DT 31-JUL-2019, integrated into UniProtKB/TrEMBL. DT 31-JUL-2019, sequence version 1. DT 25-MAY-2022, entry version 8. DE RecName: Full=tRNA pseudouridine synthase D {ECO:0000256|HAMAP-Rule:MF_01082}; DE EC=5.4.99.27 {ECO:0000256|HAMAP-Rule:MF_01082}; DE AltName: Full=tRNA pseudouridine(13) synthase {ECO:0000256|HAMAP-Rule:MF_01082}; DE AltName: Full=tRNA pseudouridylate synthase D {ECO:0000256|HAMAP-Rule:MF_01082}; DE AltName: Full=tRNA-uridine isomerase D {ECO:0000256|HAMAP-Rule:MF_01082}; GN Name=truD {ECO:0000256|HAMAP-Rule:MF_01082, GN ECO:0000313|EMBL:TKA91024.1}; GN ORFNames=FA869_12275 {ECO:0000313|EMBL:TKA91024.1}; OS Halopseudomonas bauzanensis. OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; OC Pseudomonadaceae; Halopseudomonas. OX NCBI_TaxID=653930 {ECO:0000313|EMBL:TKA91024.1, ECO:0000313|Proteomes:UP000305198}; RN [1] {ECO:0000313|EMBL:TKA91024.1, ECO:0000313|Proteomes:UP000305198} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=SBBB {ECO:0000313|EMBL:TKA91024.1, RC ECO:0000313|Proteomes:UP000305198}; RA Bhattacharya S., Roy C., Mondal N., Sarkar J., Mandal S., Rameez M.J., RA Ghosh W.; RT "Crypto-aerobic microbial life in anoxic (sulfidic) marine sediments."; RL Submitted (APR-2019) to the EMBL/GenBank/DDBJ databases. CC -!- FUNCTION: Responsible for synthesis of pseudouridine from uracil-13 in CC transfer RNAs. {ECO:0000256|HAMAP-Rule:MF_01082}. CC -!- CATALYTIC ACTIVITY: CC Reaction=uridine(13) in tRNA = pseudouridine(13) in tRNA; CC Xref=Rhea:RHEA:42540, Rhea:RHEA-COMP:10105, Rhea:RHEA-COMP:10106, CC ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.27; CC Evidence={ECO:0000256|HAMAP-Rule:MF_01082}; CC -!- SIMILARITY: Belongs to the pseudouridine synthase TruD family. CC {ECO:0000256|ARBA:ARBA00007953, ECO:0000256|HAMAP-Rule:MF_01082}. CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ CC whole genome shotgun (WGS) entry which is preliminary data. CC {ECO:0000313|EMBL:TKA91024.1}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; SWAV01000004; TKA91024.1; -; Genomic_DNA. DR Proteomes; UP000305198; Unassembled WGS sequence. DR GO; GO:0003723; F:RNA binding; IEA:InterPro. DR GO; GO:0106029; F:tRNA pseudouridine synthase activity; IEA:UniProtKB-EC. DR GO; GO:0031119; P:tRNA pseudouridine synthesis; IEA:UniProtKB-UniRule. DR Gene3D; 3.30.2340.10; -; 1. DR Gene3D; 3.30.2350.20; -; 1. DR HAMAP; MF_01082; TruD; 1. DR InterPro; IPR020103; PsdUridine_synth_cat_dom_sf. DR InterPro; IPR001656; PsdUridine_synth_TruD. DR InterPro; IPR020119; PsdUridine_synth_TruD_CS. DR InterPro; IPR011760; PsdUridine_synth_TruD_insert. DR InterPro; IPR042214; TruD_catalytic. DR InterPro; IPR043165; TruD_insert_sf. DR Pfam; PF01142; TruD; 2. DR SUPFAM; SSF55120; SSF55120; 1. DR PROSITE; PS50984; TRUD; 1. DR PROSITE; PS01268; UPF0024; 1. PE 3: Inferred from homology; KW Isomerase {ECO:0000256|ARBA:ARBA00023235, ECO:0000256|HAMAP-Rule:MF_01082}; KW tRNA processing {ECO:0000256|ARBA:ARBA00022694, ECO:0000256|HAMAP- KW Rule:MF_01082}. FT DOMAIN 157..303 FT /note="TRUD" FT /evidence="ECO:0000259|PROSITE:PS50984" FT ACT_SITE 81 FT /note="Nucleophile" FT /evidence="ECO:0000256|HAMAP-Rule:MF_01082" SQ SEQUENCE 352 AA; 39244 MW; 167AF0C95B436FE6 CRC64; MDDSQWLGPR ALGEPCGRAV LKAQPEDFRV TEILDIELSG QGEHLWLLIE KRDLNTEEVA RQLARAAGIK LRDVSYAGIK DRRAITSQWF SLQLPGKADP DLSALWDDNL RCLASQRHSR KLQRGAHRAN RFVIRLSSLQ AEQESLDARL QCIATTGVPN YFGPQRFGRD GGNIDQARHW AERGGLPPAR GTRSRLLSTA RSLVFNRVLA QRVEKGNWNH LVEGDCLAFT DSRSHFSAQR LAADDRRLDE LDVHPTGPLW GQGELPTAQT AQMLEQAVAA SEPVLCHWLE SAGLEQQRRI LRLPISGLTW HYPSPDCLEI EFTLPTGCFA TAVLRELLVL ADEPGGGLES ET //