ID A0A4U0YMJ6_9PSED Unreviewed; 352 AA. AC A0A4U0YMJ6; DT 31-JUL-2019, integrated into UniProtKB/TrEMBL. DT 31-JUL-2019, sequence version 1. DT 16-OCT-2019, entry version 3. DE RecName: Full=tRNA pseudouridine synthase D {ECO:0000256|HAMAP-Rule:MF_01082, ECO:0000256|SAAS:SAAS01167837}; DE EC=5.4.99.27 {ECO:0000256|HAMAP-Rule:MF_01082}; DE AltName: Full=tRNA pseudouridine(13) synthase {ECO:0000256|HAMAP-Rule:MF_01082}; DE AltName: Full=tRNA pseudouridylate synthase D {ECO:0000256|HAMAP-Rule:MF_01082}; DE AltName: Full=tRNA-uridine isomerase D {ECO:0000256|HAMAP-Rule:MF_01082}; GN Name=truD {ECO:0000256|HAMAP-Rule:MF_01082, GN ECO:0000313|EMBL:TKA91024.1}; GN ORFNames=FA869_12275 {ECO:0000313|EMBL:TKA91024.1}; OS Pseudomonas bauzanensis. OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; OC Pseudomonadaceae; Pseudomonas. OX NCBI_TaxID=653930 {ECO:0000313|EMBL:TKA91024.1}; RN [1] {ECO:0000313|EMBL:TKA91024.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=SBBB {ECO:0000313|EMBL:TKA91024.1}; RA Bhattacharya S., Roy C., Mondal N., Sarkar J., Mandal S., Rameez M.J., RA Ghosh W.; RT "Crypto-aerobic microbial life in anoxic (sulfidic) marine RT sediments."; RL Submitted (APR-2019) to the EMBL/GenBank/DDBJ databases. CC -!- FUNCTION: Responsible for synthesis of pseudouridine from uracil- CC 13 in transfer RNAs. {ECO:0000256|HAMAP-Rule:MF_01082, CC ECO:0000256|SAAS:SAAS01167839}. CC -!- CATALYTIC ACTIVITY: CC Reaction=uridine(13) in tRNA = pseudouridine(13) in tRNA; CC Xref=Rhea:RHEA:42540, Rhea:RHEA-COMP:10105, Rhea:RHEA- CC COMP:10106, ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.27; CC Evidence={ECO:0000256|HAMAP-Rule:MF_01082}; CC -!- SIMILARITY: Belongs to the pseudouridine synthase TruD family. CC {ECO:0000256|HAMAP-Rule:MF_01082}. CC -!- CAUTION: The sequence shown here is derived from an CC EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is CC preliminary data. {ECO:0000313|EMBL:TKA91024.1}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; SWAV01000004; TKA91024.1; -; Genomic_DNA. DR GO; GO:0106029; F:tRNA pseudouridine synthase activity; IEA:UniProtKB-EC. DR GO; GO:0031119; P:tRNA pseudouridine synthesis; IEA:UniProtKB-UniRule. DR Gene3D; 3.30.2350.20; -; 1. DR HAMAP; MF_01082; TruD; 1. DR InterPro; IPR020103; PsdUridine_synth_cat_dom_sf. DR InterPro; IPR001656; PsdUridine_synth_TruD. DR InterPro; IPR020119; PsdUridine_synth_TruD_CS. DR InterPro; IPR011760; PsdUridine_synth_TruD_insert. DR InterPro; IPR042214; TruD_catalytic. DR Pfam; PF01142; TruD; 2. DR SUPFAM; SSF55120; SSF55120; 1. DR PROSITE; PS50984; TRUD; 1. DR PROSITE; PS01268; UPF0024; 1. PE 3: Inferred from homology; KW Isomerase {ECO:0000256|HAMAP-Rule:MF_01082, KW ECO:0000313|EMBL:TKA91024.1}; KW tRNA processing {ECO:0000256|HAMAP-Rule:MF_01082}. FT DOMAIN 157 303 TRUD. {ECO:0000259|PROSITE:PS50984}. FT ACT_SITE 81 81 Nucleophile. {ECO:0000256|HAMAP-Rule: FT MF_01082}. SQ SEQUENCE 352 AA; 39244 MW; 167AF0C95B436FE6 CRC64; MDDSQWLGPR ALGEPCGRAV LKAQPEDFRV TEILDIELSG QGEHLWLLIE KRDLNTEEVA RQLARAAGIK LRDVSYAGIK DRRAITSQWF SLQLPGKADP DLSALWDDNL RCLASQRHSR KLQRGAHRAN RFVIRLSSLQ AEQESLDARL QCIATTGVPN YFGPQRFGRD GGNIDQARHW AERGGLPPAR GTRSRLLSTA RSLVFNRVLA QRVEKGNWNH LVEGDCLAFT DSRSHFSAQR LAADDRRLDE LDVHPTGPLW GQGELPTAQT AQMLEQAVAA SEPVLCHWLE SAGLEQQRRI LRLPISGLTW HYPSPDCLEI EFTLPTGCFA TAVLRELLVL ADEPGGGLES ET //