ID A0A4S9UFT7_AURPU Unreviewed; 545 AA. AC A0A4S9UFT7; DT 31-JUL-2019, integrated into UniProtKB/TrEMBL. DT 31-JUL-2019, sequence version 1. DT 29-MAY-2024, entry version 15. DE RecName: Full=Tyrosine--tRNA ligase {ECO:0000256|ARBA:ARBA00013160, ECO:0000256|RuleBase:RU361234}; DE EC=6.1.1.1 {ECO:0000256|ARBA:ARBA00013160, ECO:0000256|RuleBase:RU361234}; DE AltName: Full=Tyrosyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00033323, ECO:0000256|RuleBase:RU361234}; GN ORFNames=D6C86_08051 {ECO:0000313|EMBL:THZ56168.1}, D6D22_05450 GN {ECO:0000313|EMBL:THW41314.1}; OS Aureobasidium pullulans (Black yeast) (Pullularia pullulans). OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Dothideomycetes; OC Dothideomycetidae; Dothideales; Saccotheciaceae; Aureobasidium. OX NCBI_TaxID=5580 {ECO:0000313|EMBL:THZ56168.1, ECO:0000313|Proteomes:UP000310756}; RN [1] {ECO:0000313|Proteomes:UP000310687, ECO:0000313|Proteomes:UP000310756} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=EXF-11013 {ECO:0000313|EMBL:THW41314.1, RC ECO:0000313|Proteomes:UP000310687}, and EXF-3645 RC {ECO:0000313|EMBL:THZ56168.1, ECO:0000313|Proteomes:UP000310756}; RA Gostincar C., Turk M., Zajc J., Gunde-Cimerman N.; RT "Fifty Aureobasidium pullulans genomes reveal a recombining RT polyextremotolerant generalist."; RL Submitted (OCT-2018) to the EMBL/GenBank/DDBJ databases. CC -!- CATALYTIC ACTIVITY: CC Reaction=ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + H(+) + L- CC tyrosyl-tRNA(Tyr); Xref=Rhea:RHEA:10220, Rhea:RHEA-COMP:9706, CC Rhea:RHEA-COMP:9707, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, CC ChEBI:CHEBI:33019, ChEBI:CHEBI:58315, ChEBI:CHEBI:78442, CC ChEBI:CHEBI:78536, ChEBI:CHEBI:456215; EC=6.1.1.1; CC Evidence={ECO:0000256|ARBA:ARBA00000069, CC ECO:0000256|RuleBase:RU361234}; CC -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family. CC {ECO:0000256|RuleBase:RU361234}. CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ CC whole genome shotgun (WGS) entry which is preliminary data. CC {ECO:0000313|EMBL:THZ56168.1}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; QZAL01000071; THW41314.1; -; Genomic_DNA. DR EMBL; QZBR01000070; THZ56168.1; -; Genomic_DNA. DR AlphaFoldDB; A0A4S9UFT7; -. DR Proteomes; UP000310687; Unassembled WGS sequence. DR Proteomes; UP000310756; Unassembled WGS sequence. DR GO; GO:0005829; C:cytosol; IEA:TreeGrafter. DR GO; GO:0005739; C:mitochondrion; IEA:TreeGrafter. DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW. DR GO; GO:0003723; F:RNA binding; IEA:InterPro. DR GO; GO:0004831; F:tyrosine-tRNA ligase activity; IEA:UniProtKB-EC. DR GO; GO:0006437; P:tyrosyl-tRNA aminoacylation; IEA:InterPro. DR CDD; cd00805; TyrRS_core; 1. DR Gene3D; 3.40.50.620; HUPs; 1. DR Gene3D; 3.10.290.10; RNA-binding S4 domain; 1. DR Gene3D; 1.10.240.10; Tyrosyl-Transfer RNA Synthetase; 1. DR InterPro; IPR001412; aa-tRNA-synth_I_CS. DR InterPro; IPR002305; aa-tRNA-synth_Ic. DR InterPro; IPR014729; Rossmann-like_a/b/a_fold. DR InterPro; IPR036986; S4_RNA-bd_sf. DR InterPro; IPR002307; Tyr-tRNA-ligase. DR InterPro; IPR024088; Tyr-tRNA-ligase_bac-type. DR InterPro; IPR032005; TyrRSs_C. DR NCBIfam; TIGR00234; tyrS; 1. DR PANTHER; PTHR11766:SF0; TYROSINE--TRNA LIGASE, MITOCHONDRIAL; 1. DR PANTHER; PTHR11766; TYROSYL-TRNA SYNTHETASE; 1. DR Pfam; PF00579; tRNA-synt_1b; 1. DR Pfam; PF16714; TyrRSs_C; 1. DR PRINTS; PR01040; TRNASYNTHTYR. DR SUPFAM; SSF52374; Nucleotidylyl transferase; 1. DR PROSITE; PS00178; AA_TRNA_LIGASE_I; 1. PE 3: Inferred from homology; KW Aminoacyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00023146, KW ECO:0000256|RuleBase:RU361234}; KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|RuleBase:RU361234}; KW Ligase {ECO:0000256|ARBA:ARBA00022598, ECO:0000256|RuleBase:RU361234}; KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, KW ECO:0000256|RuleBase:RU361234}; KW Protein biosynthesis {ECO:0000256|ARBA:ARBA00022917, KW ECO:0000256|RuleBase:RU361234}. FT DOMAIN 424..534 FT /note="Tyrosyl-tRNA synthetase C-terminal" FT /evidence="ECO:0000259|Pfam:PF16714" SQ SEQUENCE 545 AA; 60167 MW; BAB03273E3B43499 CRC64; MRGPTLWRSR SLQPLKPYVC GDCVSKLGLR RSSQNAVAGS NDALKSWDEQ AQKTRAGDRK SLLTILEERG YVKDLAGDRN ALDKLLTDKR IGAYSGIDPT APSLHLGHLL PMMVLFWMYI HGHRAVSIIG GATAKIGDPT GRLVSRQDMD RNTRSSNLVS LHNCVRGMWK SVERYAERHG YPKDKAWSKS IQNNSVWLGK LNIIDFLSDA GTVARMGAML GRDTVKNKMA QGDGMSYAEF TYPLLQAYDW WHLYQSGVQV QIGGSDQYGN IVAGIDLIKH LTPRPSTSPE TKQLSGPFGL TVPLLTTAAG AKFGKSAGNA IWLDKTMTSP FELYGFLVRS ADADVERYLK LFTFLPLEHI AAVMTEHEAD PSQRKAQHLL AREVVDLVHG TEEAQNTQNQ HTFSRRPNLQ SLQAEAENSK DSLNRLYLPQ SLVQGKQPAR VLYHAGLAKS NSDGTRMVNS GGAYIGSQTS EQEAATEGEL KFRSLKGVTS SVITEMVEKS NVLVLRTGKW NVKLVEVISD AEFEQKGLSA PGWEEFKEAR DASSS //