ID A0A4P6UMD1_9BURK Unreviewed; 1169 AA. AC A0A4P6UMD1; DT 31-JUL-2019, integrated into UniProtKB/TrEMBL. DT 31-JUL-2019, sequence version 1. DT 29-MAY-2024, entry version 16. DE RecName: Full=DNA polymerase III subunit alpha {ECO:0000256|ARBA:ARBA00019114}; DE EC=2.7.7.7 {ECO:0000256|ARBA:ARBA00012417}; GN ORFNames=DW355_11335 {ECO:0000313|EMBL:QBK05265.1}; OS Hylemonella gracilis. OC Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales; OC Comamonadaceae; Hylemonella. OX NCBI_TaxID=80880 {ECO:0000313|EMBL:QBK05265.1, ECO:0000313|Proteomes:UP000292939}; RN [1] {ECO:0000313|EMBL:QBK05265.1, ECO:0000313|Proteomes:UP000292939} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=NS1 {ECO:0000313|EMBL:QBK05265.1, RC ECO:0000313|Proteomes:UP000292939}; RA Tyc O., Kulkarni P., Gawehns F., Hundscheid M., Zweers H., Garbeva P.; RT "Exploring interactions and the metabolic potential of the ultra-small soil RT bacteria Hylemonella gracilis."; RL Submitted (JUL-2018) to the EMBL/GenBank/DDBJ databases. CC -!- CATALYTIC ACTIVITY: CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) = CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339, CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560, CC ChEBI:CHEBI:173112; EC=2.7.7.7; CC Evidence={ECO:0000256|ARBA:ARBA00024632}; CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; CP031395; QBK05265.1; -; Genomic_DNA. DR AlphaFoldDB; A0A4P6UMD1; -. DR OrthoDB; 9803237at2; -. DR Proteomes; UP000292939; Chromosome. DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell. DR GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro. DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW. DR GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW. DR CDD; cd04485; DnaE_OBF; 1. DR CDD; cd07433; PHP_PolIIIA_DnaE1; 1. DR Gene3D; 1.10.150.870; -; 1. DR Gene3D; 1.10.10.1600; Bacterial DNA polymerase III alpha subunit, thumb domain; 1. DR Gene3D; 3.20.20.140; Metal-dependent hydrolases; 1. DR InterPro; IPR011708; DNA_pol3_alpha_NTPase_dom. DR InterPro; IPR041931; DNA_pol3_alpha_thumb_dom. DR InterPro; IPR040982; DNA_pol3_finger. DR InterPro; IPR004805; DnaE2/DnaE/PolC. DR InterPro; IPR029460; DNAPol_HHH. DR InterPro; IPR004013; PHP_dom. DR InterPro; IPR003141; Pol/His_phosphatase_N. DR InterPro; IPR016195; Pol/histidinol_Pase-like. DR InterPro; IPR049821; PolIIIA_DnaE1_PHP. DR NCBIfam; TIGR00594; polc; 1. DR PANTHER; PTHR32294; DNA POLYMERASE III SUBUNIT ALPHA; 1. DR PANTHER; PTHR32294:SF0; DNA POLYMERASE III SUBUNIT ALPHA; 1. DR Pfam; PF07733; DNA_pol3_alpha; 1. DR Pfam; PF17657; DNA_pol3_finger; 1. DR Pfam; PF14579; HHH_6; 1. DR Pfam; PF02811; PHP; 1. DR SMART; SM00481; POLIIIAc; 1. DR SUPFAM; SSF89550; PHP domain-like; 2. PE 4: Predicted; KW DNA replication {ECO:0000256|ARBA:ARBA00022705}; KW DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00022932}; KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695, KW ECO:0000313|EMBL:QBK05265.1}; KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000313|EMBL:QBK05265.1}. FT DOMAIN 7..74 FT /note="Polymerase/histidinol phosphatase N-terminal" FT /evidence="ECO:0000259|SMART:SM00481" SQ SEQUENCE 1169 AA; 129577 MW; 631FD8B3A7D33D9A CRC64; MSTPSFSHLR LHTEFSVVDG ATRVDDVVDA AAKDGQPALA ITDLNNLFGA IKFYKAARGK GVKPLIGVEI LLEGLGADAQ VTSRLLLLVQ NKQGYLNLSE LLARAWTSNV HKAQAIVKLA WLKELHEGLI VLSGAQAGAV GQALVQGDTA RAHDVALQFA TLFPHRFYLE LQRAGRSDDE AHVGAAVQLA SRLKLPVVAT HPVQFLTEDD YEAHEARVCI AEGEILGNPR RVRRFTREQY FKSAAQMQAL FADIPSALAN TLEIAKRCNL TLTLGKPQLP NFPIPGGLSI EDYFRKASLE GLEDRLALLY PDATKRDAER PRYLERLEFE INTILKMGFP GYFLIVGDFI QWAKANGCPV GPGRGSGAGS LVAYALKITD LDPLAYKLLF ERFLNPERVS MPDFDIDFCQ ANRDRVIEYV KERYGREAVS QIATFGTMAA RAAIRDVGRV MDMSYTFCDG ISKLIPNKPG QHITIDGALK EVPELAERQA KEDEVKNLLE LAQKLEGMTR NVGMHAGGVL IAPGKLTDFC PLYQQPGSDA AVSQYDKDDV EAVGLVKFDF LGLATLTILE IAREFIMKRH PGQEHFNFES IPLDDKATYQ LFQDGKTEAV FQFESRGMQG MLRDAKPTRL EDLIALNALY RPGPMDLIPS FVARKHGREE IEYPHPLVAD MLSETYGIMV YQEQVMQTAQ ILGGYSLGGA DLLRRAMGKK KAEEMAQHRE IFRKGAAGNG IAQDKADEIF DLMEKFAGYG FNKSHAAAYS LLAYHTAWLK VHYTAEFFCA NMTVEMDDTD KLKVLFEDAQ KMGMRFEPPD VNRGHYRFEP VSDSVIRYGL GAVKGTGQQA IEAIVKAREE DGPFKSLYDF CNRVDRARIN KRTVEALIKA GAFDSLNLNR AALLASIDRA FDFATATAAN ANQGGLFDLT ADDGADAHGS STQEPELVEA LPWGVKERLS HEKTAVGFYF SGHLFDEVAH EVRRFAKREL AELIDTREPQ LLAGIVSDLR VINGQRGRLA LFKLDDKSVA LDARVDEALL NANKNLLKDD ELVVVMAKVM PDRFSGGLQL NVTQLWSLEA ARCRFGKYLS VAVNLSSGRY PDVRRLLTDH PPQREMTEQG ELLRGLPVRF TLKVGSEAIG TYQSALKMED EKTRFYPSDA ALAGWMAEAD QGKAVVVYE //