ID A0A4D6SRC5_9LAMI Unreviewed; 751 AA. AC A0A4D6SRC5; DT 03-JUL-2019, integrated into UniProtKB/TrEMBL. DT 03-JUL-2019, sequence version 1. DT 16-OCT-2019, entry version 4. DE RecName: Full=NAD(P)H-quinone oxidoreductase subunit 5, chloroplastic {ECO:0000256|RuleBase:RU364062}; DE EC=7.1.1.- {ECO:0000256|RuleBase:RU364062}; DE AltName: Full=NADH-plastoquinone oxidoreductase subunit 5 {ECO:0000256|RuleBase:RU364062}; GN Name=ndhF {ECO:0000256|RuleBase:RU364062, GN ECO:0000313|EMBL:QCG69614.1}; OS Buddleja sessilifolia. OG Plastid; Chloroplast {ECO:0000313|EMBL:QCG69614.1}. OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; OC Pentapetalae; asterids; lamiids; Lamiales; Scrophulariaceae; OC Buddlejeae; Buddleja. OX NCBI_TaxID=2567862 {ECO:0000313|EMBL:QCG69614.1}; RN [1] {ECO:0000313|EMBL:QCG69614.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=GJ10 {ECO:0000313|EMBL:QCG69704.1}, and GJ9 RC {ECO:0000313|EMBL:QCG69614.1}; RA Ge J., Cai L., Bi G., Chen G., Sun W.; RT "Characterization of the complete chloroplast genomes of Buddleja RT colvilei and B. sessilifolia: Implications for the taxonomy of RT Buddleja L."; RL Molecules 23:0-1248(2018). CC -!- FUNCTION: NDH shuttles electrons from NAD(P)H:plastoquinone, via CC FMN and iron-sulfur (Fe-S) centers, to quinones in the CC photosynthetic chain and possibly in a chloroplast respiratory CC chain. The immediate electron acceptor for the enzyme in this CC species is believed to be plastoquinone. Couples the redox CC reaction to proton translocation, and thus conserves the redox CC energy in a proton gradient. {ECO:0000256|RuleBase:RU364062}. CC -!- CATALYTIC ACTIVITY: CC Reaction=a plastoquinone + (n+1) H(+)(in) + NADH = a plastoquinol CC + n H(+)(out) + NAD(+); Xref=Rhea:RHEA:42608, Rhea:RHEA- CC COMP:9561, Rhea:RHEA-COMP:9562, ChEBI:CHEBI:15378, CC ChEBI:CHEBI:17757, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, CC ChEBI:CHEBI:62192; Evidence={ECO:0000256|RuleBase:RU364062, CC ECO:0000256|SAAS:SAAS01131453}; CC -!- CATALYTIC ACTIVITY: CC Reaction=a plastoquinone + (n+1) H(+)(in) + NADPH = a plastoquinol CC + n H(+)(out) + NADP(+); Xref=Rhea:RHEA:42612, Rhea:RHEA- CC COMP:9561, Rhea:RHEA-COMP:9562, ChEBI:CHEBI:15378, CC ChEBI:CHEBI:17757, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, CC ChEBI:CHEBI:62192; Evidence={ECO:0000256|RuleBase:RU364062, CC ECO:0000256|SAAS:SAAS01131456}; CC -!- SUBUNIT: NDH is composed of at least 16 different subunits, 5 of CC which are encoded in the nucleus. {ECO:0000256|RuleBase:RU364062}. CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid membrane CC {ECO:0000256|RuleBase:RU364062}; Multi-pass membrane protein CC {ECO:0000256|RuleBase:RU364062}. CC -!- SIMILARITY: Belongs to the complex I subunit 5 family. CC {ECO:0000256|RuleBase:RU364062, ECO:0000256|SAAS:SAAS00546322}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; MH411149; QCG69614.1; -; Genomic_DNA. DR EMBL; MH411150; QCG69704.1; -; Genomic_DNA. DR GO; GO:0009535; C:chloroplast thylakoid membrane; IEA:UniProtKB-SubCell. DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW. DR GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; IEA:InterPro. DR GO; GO:0048038; F:quinone binding; IEA:UniProtKB-KW. DR GO; GO:0042773; P:ATP synthesis coupled electron transport; IEA:InterPro. DR InterPro; IPR002128; NADH_UbQ_OxRdtase_chlpt_su5_C. DR InterPro; IPR003945; NADHpl_OxRdtase_5. DR InterPro; IPR018393; NADHpl_OxRdtase_5_subgr. DR InterPro; IPR001750; ND/Mrp_mem. DR InterPro; IPR001516; Proton_antipo_N. DR Pfam; PF01010; Proton_antipo_C; 1. DR Pfam; PF00361; Proton_antipo_M; 1. DR Pfam; PF00662; Proton_antipo_N; 1. DR PRINTS; PR01435; NPOXDRDTASE5. DR TIGRFAMs; TIGR01974; NDH_I_L; 1. PE 3: Inferred from homology; KW Chloroplast {ECO:0000256|RuleBase:RU364062, KW ECO:0000313|EMBL:QCG69614.1}; KW Membrane {ECO:0000256|RuleBase:RU364062, KW ECO:0000256|SAAS:SAAS00464283}; KW NAD {ECO:0000256|RuleBase:RU364062, ECO:0000256|SAAS:SAAS00220887}; KW NADP {ECO:0000256|RuleBase:RU364062, ECO:0000256|SAAS:SAAS00093259}; KW Plastid {ECO:0000256|RuleBase:RU364062, ECO:0000313|EMBL:QCG69614.1}; KW Plastoquinone {ECO:0000256|RuleBase:RU364062, KW ECO:0000256|SAAS:SAAS00430364}; KW Quinone {ECO:0000256|RuleBase:RU364062}; KW Thylakoid {ECO:0000256|RuleBase:RU364062}; KW Translocase {ECO:0000256|SAAS:SAAS01131454}; KW Transmembrane {ECO:0000256|RuleBase:RU364062, KW ECO:0000256|SAAS:SAAS00464191}; KW Transmembrane helix {ECO:0000256|RuleBase:RU364062, KW ECO:0000256|SAAS:SAAS00464492}; KW Transport {ECO:0000256|RuleBase:RU364062}. FT TRANSMEM 6 28 Helical. {ECO:0000256|RuleBase:RU364062}. FT TRANSMEM 40 60 Helical. {ECO:0000256|RuleBase:RU364062}. FT TRANSMEM 85 109 Helical. {ECO:0000256|RuleBase:RU364062}. FT TRANSMEM 121 139 Helical. {ECO:0000256|RuleBase:RU364062}. FT TRANSMEM 145 166 Helical. {ECO:0000256|RuleBase:RU364062}. FT TRANSMEM 187 205 Helical. {ECO:0000256|RuleBase:RU364062}. FT TRANSMEM 262 287 Helical. {ECO:0000256|RuleBase:RU364062}. FT TRANSMEM 293 311 Helical. {ECO:0000256|RuleBase:RU364062}. FT TRANSMEM 318 339 Helical. {ECO:0000256|RuleBase:RU364062}. FT TRANSMEM 393 412 Helical. {ECO:0000256|RuleBase:RU364062}. FT TRANSMEM 424 447 Helical. {ECO:0000256|RuleBase:RU364062}. FT TRANSMEM 548 568 Helical. {ECO:0000256|RuleBase:RU364062}. FT TRANSMEM 606 624 Helical. {ECO:0000256|RuleBase:RU364062}. FT TRANSMEM 723 741 Helical. {ECO:0000256|RuleBase:RU364062}. FT DOMAIN 72 129 Proton_antipo_N. {ECO:0000259|Pfam: FT PF00662}. FT DOMAIN 141 442 Proton_antipo_M. {ECO:0000259|Pfam: FT PF00361}. FT DOMAIN 448 690 Proton_antipo_C. {ECO:0000259|Pfam: FT PF01010}. SQ SEQUENCE 751 AA; 85428 MW; 7499F28057B7130A CRC64; MEQTYQYAWI IPFLPLPVPM LIGVGLLLFP TATKNLRRMW AFPSILLLSI VMIFSTNLSI QQINSSFIYQ YVWSWTLDND FSLEFGYLID PLTSIMLMLI TTVGIMVLIY SDNYMAHDQG YLRFFAYMSF FSTSMLGLVT SSNLIQIYIF WELVGMCSYL LIGFWFTRPP AANACQKAFV TNRVGDFGLL LGILGFYWIT GSFEFRDLFE IFNNLIYNNE VNSPFVTLCA ALLFAGAVAK SAQFPLHVWL PDAMEGPTPI SALIHAATMV AAGIFLVARL LPLFILIPYI MNLISLIGII TVLLGATLAL AQKDIKRGLA YSTMSQLGYM MLALGMGSYR SALFHLITHA YSKALLFLGS GSVIHSMETI VGYSPDKSQN MVLMGGLTKH IPITKTSFLL GTLSLCGIPP LACFWSKDEI LNDSWLYSPI FAIIAWATAG LTSFYMFRIY FLTFEGHLNS NFQNYSGSQN TPLYSISLWG KGCSKRINKS FRLLRMNNNE SSSFLSKTTY RSDDNVRKRN RGRPFINIVH FDNKKPYSYP YESDNTMLFP LLVLVLFTLF VGILGIPFNQ EGTDLDILSK WLAPSINLLH QKSKDSADWY EFFKDAIFSV SIAYFGIFLA SFLYKPIYSS FKNFYLINLF VKIGPKRRYW DKILNVLYDW SYNRAYIDAF YTTSFTEAIR GVAQLTHFFD RRVIDGITNG VGVMSFFVGE GIKYVGGGRI SSYLFLYFSC VSIFLLVYFF IPFDITFESF I //