ID   A0A424S9H7_9PROT        Unreviewed;       438 AA.
AC   A0A424S9H7;
DT   08-MAY-2019, integrated into UniProtKB/TrEMBL.
DT   08-MAY-2019, sequence version 1.
DT   13-SEP-2023, entry version 14.
DE   RecName: Full=Proline--tRNA ligase {ECO:0000256|ARBA:ARBA00019110};
DE            EC=6.1.1.15 {ECO:0000256|ARBA:ARBA00012831};
DE   AltName: Full=Prolyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00029731};
GN   ORFNames=CBD13_003125 {ECO:0000313|EMBL:RPH00430.1};
OS   Candidatus Pelagibacter sp. TMED153.
OC   Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus Pelagibacterales;
OC   Pelagibacteraceae; Candidatus Pelagibacter.
OX   NCBI_TaxID=1986803 {ECO:0000313|EMBL:RPH00430.1};
RN   [1] {ECO:0000313|EMBL:RPH00430.1, ECO:0000313|Proteomes:UP000195484}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=TMED153 {ECO:0000313|EMBL:RPH00430.1};
RX   PubMed=28713657; DOI=10.7717/peerj.3558;
RA   Tully B.J., Sachdeva R., Graham E.D., Heidelberg J.F.;
RT   "290 metagenome-assembled genomes from the Mediterranean Sea: a resource
RT   for marine microbiology.";
RL   PeerJ 5:e3558-e3558(2017).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-
CC         tRNA(Pro); Xref=Rhea:RHEA:14305, Rhea:RHEA-COMP:9700, Rhea:RHEA-
CC         COMP:9702, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:60039,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78532, ChEBI:CHEBI:456215;
CC         EC=6.1.1.15; Evidence={ECO:0000256|ARBA:ARBA00000857};
CC   -!- SUBUNIT: Homodimer. {ECO:0000256|ARBA:ARBA00011738}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:RPH00430.1}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; NHHC02000046; RPH00430.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A424S9H7; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004827; F:proline-tRNA ligase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006433; P:prolyl-tRNA aminoacylation; IEA:InterPro.
DR   CDD; cd00861; ProRS_anticodon_short; 1.
DR   CDD; cd00779; ProRS_core_prok; 1.
DR   Gene3D; 3.40.50.800; Anticodon-binding domain; 1.
DR   InterPro; IPR002314; aa-tRNA-synt_IIb.
DR   InterPro; IPR006195; aa-tRNA-synth_II.
DR   InterPro; IPR045864; aa-tRNA-synth_II/BPL/LPL.
DR   InterPro; IPR004154; Anticodon-bd.
DR   InterPro; IPR036621; Anticodon-bd_dom_sf.
DR   InterPro; IPR002316; Pro-tRNA-ligase_IIa.
DR   InterPro; IPR004500; Pro-tRNA-synth_IIa_bac-type.
DR   InterPro; IPR044140; ProRS_anticodon_short.
DR   InterPro; IPR033730; ProRS_core_prok.
DR   NCBIfam; TIGR00409; proS_fam_II; 1.
DR   PANTHER; PTHR42753; MITOCHONDRIAL RIBOSOME PROTEIN L39/PROLYL-TRNA LIGASE FAMILY MEMBER; 1.
DR   PANTHER; PTHR42753:SF2; PROLYL-TRNA SYNTHETASE; 1.
DR   Pfam; PF03129; HGTP_anticodon; 1.
DR   Pfam; PF00587; tRNA-synt_2b; 1.
DR   PRINTS; PR01046; TRNASYNTHPRO.
DR   SUPFAM; SSF52954; Class II aaRS ABD-related; 1.
DR   SUPFAM; SSF55681; Class II aaRS and biotin synthetases; 1.
DR   PROSITE; PS50862; AA_TRNA_LIGASE_II; 1.
PE   4: Predicted;
KW   Aminoacyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00023146};
KW   ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW   Coiled coil {ECO:0000256|SAM:Coils};
KW   Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW   Ligase {ECO:0000256|ARBA:ARBA00022598, ECO:0000313|EMBL:RPH00430.1};
KW   Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW   Protein biosynthesis {ECO:0000256|ARBA:ARBA00022917}.
FT   DOMAIN          38..339
FT                   /note="Aminoacyl-transfer RNA synthetases class-II family
FT                   profile"
FT                   /evidence="ECO:0000259|PROSITE:PS50862"
FT   COILED          354..381
FT                   /evidence="ECO:0000256|SAM:Coils"
SQ   SEQUENCE   438 AA;  50890 MW;  AAABF1D782CBDA9A CRC64;
     MYLSKLFIPI TKDLPAEAKI KSHQLMLRAG MIKQSSAGIY SWLPLGFKVM KKIEQIVREE
     QNLIGAQEML MPTIQSSEIW KESGRYKDYG EEMLRIKDRQ GREMLYGPTN EELVTDIFRS
     SIKSYKSLPQ ILYHIQWKFR DEIRPRFGVM RCREFYMKDA YSFDLSDEDA KKSYNKMFYS
     YIRTFSRLGL KAIPMSADTG PIGGDLSHEF IILAETGESQ IYADKKIFEI DLNKYDFSDK
     SLQKMREDFS SIYAVTDDKY KETDFNKKVK KENQIKTKGI EVGHIFYFSD KYSKPLSCLI
     DTQGGKKIAV KMGSYGIGIS RLVGATIEAN YNKNVMKWPK SISPFDVVII PSISKNNNEN
     MEKAEKIYKE LKKQNIDVLL DDVDESMSNK FKKHDLIGIP YQIIIGSKSE QDKFEFKELN
     SKNEMLNLEN IKSQLKKK
//