ID A0A417A2J2_9FIRM Unreviewed; 224 AA. AC A0A417A2J2; DT 08-MAY-2019, integrated into UniProtKB/TrEMBL. DT 08-MAY-2019, sequence version 1. DT 22-FEB-2023, entry version 8. DE RecName: Full=Aminopyrimidine aminohydrolase {ECO:0000256|ARBA:ARBA00013647}; DE EC=3.5.99.2 {ECO:0000256|ARBA:ARBA00012684}; GN ORFNames=DW958_18435 {ECO:0000313|EMBL:RHS59746.1}; OS Ruminococcus sp. AM46-18. OC Bacteria; Firmicutes; Clostridia; Eubacteriales; Oscillospiraceae; OC Ruminococcus. OX NCBI_TaxID=2293220 {ECO:0000313|EMBL:RHS59746.1, ECO:0000313|Proteomes:UP000284979}; RN [1] {ECO:0000313|EMBL:RHS59746.1, ECO:0000313|Proteomes:UP000284979} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=AM46-18 {ECO:0000313|EMBL:RHS59746.1, RC ECO:0000313|Proteomes:UP000284979}; RA Zou Y., Xue W., Luo G.; RT "A genome reference for cultivated species of the human gut microbiota."; RL Submitted (AUG-2018) to the EMBL/GenBank/DDBJ databases. CC -!- CATALYTIC ACTIVITY: CC Reaction=4-amino-5-aminomethyl-2-methylpyrimidine + H2O = 4-amino-5- CC hydroxymethyl-2-methylpyrimidine + NH4(+); Xref=Rhea:RHEA:31799, CC ChEBI:CHEBI:15377, ChEBI:CHEBI:16892, ChEBI:CHEBI:28938, CC ChEBI:CHEBI:63416; EC=3.5.99.2; CC Evidence={ECO:0000256|ARBA:ARBA00001881}; CC -!- CATALYTIC ACTIVITY: CC Reaction=H2O + thiamine = 4-amino-5-hydroxymethyl-2-methylpyrimidine + CC 5-(2-hydroxyethyl)-4-methylthiazole + H(+); Xref=Rhea:RHEA:17509, CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:16892, CC ChEBI:CHEBI:17957, ChEBI:CHEBI:18385; EC=3.5.99.2; CC Evidence={ECO:0000256|ARBA:ARBA00001671}; CC -!- PATHWAY: Cofactor biosynthesis; thiamine diphosphate biosynthesis. CC {ECO:0000256|ARBA:ARBA00004948}. CC -!- SUBUNIT: Homotetramer. {ECO:0000256|ARBA:ARBA00011881}. CC -!- SIMILARITY: Belongs to the TenA family. CC {ECO:0000256|ARBA:ARBA00010264}. CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ CC whole genome shotgun (WGS) entry which is preliminary data. CC {ECO:0000313|EMBL:RHS59746.1}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; QUIC01000063; RHS59746.1; -; Genomic_DNA. DR RefSeq; WP_055200141.1; NZ_QUIC01000063.1. DR AlphaFoldDB; A0A417A2J2; -. DR UniPathway; UPA00060; -. DR Proteomes; UP000284979; Unassembled WGS sequence. DR GO; GO:0050334; F:thiaminase activity; IEA:UniProtKB-EC. DR GO; GO:0009229; P:thiamine diphosphate biosynthetic process; IEA:UniProtKB-UniPathway. DR CDD; cd19369; TenA_C-like; 1. DR Gene3D; 1.20.910.10; Heme oxygenase-like; 1. DR InterPro; IPR016084; Haem_Oase-like_multi-hlx. DR InterPro; IPR004305; Thiaminase-2/PQQC. DR PANTHER; PTHR43198; BIFUNCTIONAL TH2 PROTEIN; 1. DR PANTHER; PTHR43198:SF2; SI:CH1073-67J19.1-RELATED; 1. DR Pfam; PF03070; TENA_THI-4; 1. DR SUPFAM; SSF48613; Heme oxygenase-like; 1. PE 3: Inferred from homology; FT DOMAIN 13..215 FT /note="Thiaminase-2/PQQC" FT /evidence="ECO:0000259|Pfam:PF03070" SQ SEQUENCE 224 AA; 26468 MW; 23EF2B5C92E745B4 CRC64; MAFMKDILTR NIPIWEECAA TPFVQEVQTG KLPLEKFKRY MIQDSIYLKN YARIYGKAIF HADTLREIQL YYSMLNFVND TESEVRLDYL KQFCMTDNDI ELIAPLPENQ NYIDFMFEIA SHGKNEEILM AVLPCMLSYS YIFRKLAAVP TSRQSRYWDF IKDYADEQYA ESCKEWSAFA EHKCAGLSVA NKKYLADIFE KASLLELAFW KMAYRNERME ENAK //