ID A0A401NEE6_RHOER Unreviewed; 212 AA. AC A0A401NEE6; DT 08-MAY-2019, integrated into UniProtKB/TrEMBL. DT 08-MAY-2019, sequence version 1. DT 13-SEP-2023, entry version 12. DE RecName: Full=Nitrile hydratase subunit beta {ECO:0000256|PIRNR:PIRNR001427}; DE Short=NHase {ECO:0000256|PIRNR:PIRNR001427}; DE EC=4.2.1.84 {ECO:0000256|PIRNR:PIRNR001427}; GN ORFNames=rerp_57550 {ECO:0000313|EMBL:GCB59347.1}; OS Rhodococcus erythropolis (Arthrobacter picolinophilus). OC Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales; Nocardiaceae; OC Rhodococcus; Rhodococcus erythropolis group. OX NCBI_TaxID=1833 {ECO:0000313|EMBL:GCB59347.1, ECO:0000313|Proteomes:UP000287380}; RN [1] {ECO:0000313|Proteomes:UP000287380} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=JCM 3201 {ECO:0000313|Proteomes:UP000287380}; RA Yoshida K., Kitagawa W., Ishiya K., Mitani Y., Nakashima N., Aburatani S., RA Tamura T.; RT "Genome sequence of Rhodococcus erythropolis type strain, JCM 3201."; RL Submitted (OCT-2018) to the EMBL/GenBank/DDBJ databases. CC -!- FUNCTION: NHase catalyzes the hydration of various nitrile compounds to CC the corresponding amides. {ECO:0000256|ARBA:ARBA00004042, CC ECO:0000256|PIRNR:PIRNR001427}. CC -!- CATALYTIC ACTIVITY: CC Reaction=an aliphatic amide = a nitrile + H2O; Xref=Rhea:RHEA:12673, CC ChEBI:CHEBI:15377, ChEBI:CHEBI:18379, ChEBI:CHEBI:65285; EC=4.2.1.84; CC Evidence={ECO:0000256|ARBA:ARBA00001410, CC ECO:0000256|PIRNR:PIRNR001427}; CC -!- SIMILARITY: Belongs to the nitrile hydratase subunit beta family. CC {ECO:0000256|ARBA:ARBA00009098, ECO:0000256|PIRNR:PIRNR001427}. CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ CC whole genome shotgun (WGS) entry which is preliminary data. CC {ECO:0000313|EMBL:GCB59347.1}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; BHXB01000001; GCB59347.1; -; Genomic_DNA. DR RefSeq; WP_060939365.1; NZ_JAHWLW010000038.1. DR AlphaFoldDB; A0A401NEE6; -. DR Proteomes; UP000287380; Unassembled WGS sequence. DR GO; GO:0080109; F:indole-3-acetonitrile nitrile hydratase activity; IEA:UniProtKB-UniRule. DR GO; GO:0046914; F:transition metal ion binding; IEA:InterPro. DR GO; GO:0006807; P:nitrogen compound metabolic process; IEA:InterPro. DR Gene3D; 2.30.30.50; -; 1. DR Gene3D; 1.10.472.20; Nitrile hydratase, beta subunit; 1. DR InterPro; IPR042262; CN_hydtase_beta_C. DR InterPro; IPR024690; CN_hydtase_beta_dom. DR InterPro; IPR008990; Elect_transpt_acc-like_dom_sf. DR InterPro; IPR003168; Nitrile_hydratase_bsu. DR NCBIfam; TIGR03888; nitrile_beta; 1. DR Pfam; PF02211; NHase_beta; 1. DR PIRSF; PIRSF001427; NHase_beta; 1. DR SUPFAM; SSF50090; Electron transport accessory proteins; 1. PE 3: Inferred from homology; KW Lyase {ECO:0000256|ARBA:ARBA00023239, ECO:0000256|PIRNR:PIRNR001427}. FT DOMAIN 1..210 FT /note="Nitrile hydratase beta subunit" FT /evidence="ECO:0000259|Pfam:PF02211" SQ SEQUENCE 212 AA; 23463 MW; 93A9E55A5EA41CC1 CRC64; MDGVHDLAGV QGFGKVPHTV NADIGPTFHA EWEHLPYSLM FAGVAELGAF SVDEVRYVVE RMEPRHYMMT SYYERYVIGV AALMVEKGIL TQEELESLAG GPFPLSRPSE SEGRPARVDT TTFEVGQRVR VRDEYVPGHI RMPAYCRGRV GTIAHRTTEK WPFPDAIGHG RNDAGEEPTY HVTFAAEELF GSDTDGGSVV VDLFEGYLEP AA //