ID A0A401HQ12_9EURY Unreviewed; 242 AA.
AC A0A401HQ12;
DT 08-MAY-2019, integrated into UniProtKB/TrEMBL.
DT 08-MAY-2019, sequence version 1.
DT 02-OCT-2024, entry version 16.
DE RecName: Full=Phosphoribosylaminoimidazole-succinocarboxamide synthase {ECO:0000256|HAMAP-Rule:MF_00137};
DE EC=6.3.2.6 {ECO:0000256|HAMAP-Rule:MF_00137};
DE AltName: Full=SAICAR synthetase {ECO:0000256|HAMAP-Rule:MF_00137};
GN Name=purC {ECO:0000256|HAMAP-Rule:MF_00137};
GN ORFNames=MHHB_P0461 {ECO:0000313|EMBL:GBF36231.1};
OS Methanofervidicoccus abyssi.
OC Archaea; Euryarchaeota; Methanomada group; Methanococci; Methanococcales;
OC Methanofervidicoccus.
OX NCBI_TaxID=2082189 {ECO:0000313|EMBL:GBF36231.1, ECO:0000313|Proteomes:UP000290527};
RN [1] {ECO:0000313|EMBL:GBF36231.1, ECO:0000313|Proteomes:UP000290527}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=HHB {ECO:0000313|EMBL:GBF36231.1,
RC ECO:0000313|Proteomes:UP000290527};
RX PubMed=30843780; DOI=.1099/ijsem.0.003297;
RA Sakai S., Takaki Y., Miyazaki M., Ogawara M., Yanagawa K., Miyazaki J.,
RA Takai K.;
RT "Methanofervidicoccus abyssi gen. nov., sp. nov., a hydrogenotrophic
RT methanogen, isolated from a hydrothermal vent chimney in the Mid-Cayman
RT Spreading Center, the Caribbean Sea.";
RL Int. J. Syst. Evol. Microbiol. 69:1225-1230(2019).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + ATP +
CC L-aspartate = (2S)-2-[5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-
CC 4-carboxamido]succinate + ADP + 2 H(+) + phosphate;
CC Xref=Rhea:RHEA:22628, ChEBI:CHEBI:15378, ChEBI:CHEBI:29991,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:58443,
CC ChEBI:CHEBI:77657, ChEBI:CHEBI:456216; EC=6.3.2.6;
CC Evidence={ECO:0000256|HAMAP-Rule:MF_00137};
CC -!- PATHWAY: Purine metabolism; IMP biosynthesis via de novo pathway; 5-
CC amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-
CC phospho-D-ribosyl)imidazole-4-carboxylate: step 1/2.
CC {ECO:0000256|ARBA:ARBA00004672, ECO:0000256|HAMAP-Rule:MF_00137}.
CC -!- SIMILARITY: Belongs to the SAICAR synthetase family.
CC {ECO:0000256|ARBA:ARBA00010190, ECO:0000256|HAMAP-Rule:MF_00137}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:GBF36231.1}.
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DR EMBL; BFAX01000002; GBF36231.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A401HQ12; -.
DR OrthoDB; 10775at2157; -.
DR UniPathway; UPA00074; UER00131.
DR Proteomes; UP000290527; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004639; F:phosphoribosylaminoimidazolesuccinocarboxamide synthase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006189; P:'de novo' IMP biosynthetic process; IEA:UniProtKB-UniRule.
DR GO; GO:0009236; P:cobalamin biosynthetic process; IEA:InterPro.
DR CDD; cd01415; SAICAR_synt_PurC; 1.
DR Gene3D; 3.30.470.20; ATP-grasp fold, B domain; 1.
DR HAMAP; MF_00137; SAICAR_synth; 1.
DR InterPro; IPR028923; SAICAR_synt/ADE2_N.
DR InterPro; IPR033934; SAICAR_synt_PurC.
DR InterPro; IPR001636; SAICAR_synth.
DR InterPro; IPR050089; SAICAR_synthetase.
DR InterPro; IPR018236; SAICAR_synthetase_CS.
DR NCBIfam; TIGR00081; purC; 1.
DR PANTHER; PTHR43599:SF3; BIFUNCTIONAL PHOSPHORIBOSYLAMINOIMIDAZOLE CARBOXYLASE_PHOSPHORIBOSYLAMINOIMIDAZOLE SUCCINOCARBOXAMIDE SYNTHETASE; 1.
DR PANTHER; PTHR43599; MULTIFUNCTIONAL PROTEIN ADE2; 1.
DR Pfam; PF01259; SAICAR_synt; 1.
DR SUPFAM; SSF56104; SAICAR synthase-like; 1.
DR PROSITE; PS01057; SAICAR_SYNTHETASE_1; 1.
DR PROSITE; PS01058; SAICAR_SYNTHETASE_2; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|HAMAP-
KW Rule:MF_00137};
KW Ligase {ECO:0000256|ARBA:ARBA00022598, ECO:0000256|HAMAP-Rule:MF_00137};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|HAMAP-
KW Rule:MF_00137};
KW Purine biosynthesis {ECO:0000256|ARBA:ARBA00022755, ECO:0000256|HAMAP-
KW Rule:MF_00137}; Reference proteome {ECO:0000313|Proteomes:UP000290527}.
FT DOMAIN 14..238
FT /note="SAICAR synthetase/ADE2 N-terminal"
FT /evidence="ECO:0000259|Pfam:PF01259"
SQ SEQUENCE 242 AA; 27721 MW; EE19A2003790C9B1 CRC64;
MDIDVKEILK REPLYSGKVK SIYEIDEDSV LIEFRDDITA GDGKKRDVKK GKGHLNALIS
SKLFEVLNKE DIPTHYLGYI DPVYMVAKKV EIIPIEVIVR NIAAGSLCKR YPFKEGEELK
RPIVQFDYKS DEYGDPMLNE DIAIALGLAT EEELKKLREL ALRVNDTLKR FFDEKGIILV
DFKIEIGRTK DGHLVVADEI SPDTMRLWDK ETKDVLDKDV FRKDLGDVVS KYEIVAKRIG
CI
//