ID A0A3S0ZT07_ELYCH Unreviewed; 663 AA. AC A0A3S0ZT07; DT 10-APR-2019, integrated into UniProtKB/TrEMBL. DT 10-APR-2019, sequence version 1. DT 12-AUG-2020, entry version 8. DE RecName: Full=Hormone-sensitive lipase {ECO:0000256|ARBA:ARBA00015845}; DE EC=3.1.1.79 {ECO:0000256|ARBA:ARBA00013088}; DE Flags: Fragment; GN ORFNames=EGW08_006196 {ECO:0000313|EMBL:RUS86042.1}; OS Elysia chlorotica (Eastern emerald elysia) (Sea slug). OC Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Mollusca; Gastropoda; OC Heterobranchia; Euthyneura; Panpulmonata; Sacoglossa; Placobranchoidea; OC Plakobranchidae; Elysia. OX NCBI_TaxID=188477 {ECO:0000313|EMBL:RUS86042.1, ECO:0000313|Proteomes:UP000271974}; RN [1] {ECO:0000313|EMBL:RUS86042.1, ECO:0000313|Proteomes:UP000271974} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=EC2010 {ECO:0000313|EMBL:RUS86042.1}; RC TISSUE=Whole organism of an adult {ECO:0000313|EMBL:RUS86042.1}; RA Cai H., Li Q., Fang X., Li J., Curtis N.E., Altenburger A., Shibata T., RA Feng M., Maeda T., Schwartz J.A., Shigenobu S., Lundholm N., Nishiyama T., RA Yang H., Hasebe M., Li S., Pierce S.K., Wang J.; RT "A draft genome assembly of the solar-powered sea slug Elysia chlorotica."; RL Submitted (JAN-2019) to the EMBL/GenBank/DDBJ databases. CC -!- FUNCTION: In adipose tissue and heart, it primarily hydrolyzes stored CC triglycerides to free fatty acids, while in steroidogenic tissues, it CC principally converts cholesteryl esters to free cholesterol for steroid CC hormone production. {ECO:0000256|ARBA:ARBA00002419}. CC -!- CATALYTIC ACTIVITY: CC Reaction=a diacylglycerol + H2O = a fatty acid + a monoacylglycerol + CC H(+); Xref=Rhea:RHEA:32731, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, CC ChEBI:CHEBI:17408, ChEBI:CHEBI:18035, ChEBI:CHEBI:28868; EC=3.1.1.79; CC Evidence={ECO:0000256|ARBA:ARBA00000354}; CC -!- CATALYTIC ACTIVITY: CC Reaction=a monoacylglycerol + H2O = a fatty acid + glycerol + H(+); CC Xref=Rhea:RHEA:15245, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, CC ChEBI:CHEBI:17408, ChEBI:CHEBI:17754, ChEBI:CHEBI:28868; EC=3.1.1.79; CC Evidence={ECO:0000256|ARBA:ARBA00001413}; CC -!- CATALYTIC ACTIVITY: CC Reaction=a triacylglycerol + H2O = a diacylglycerol + a fatty acid + CC H(+); Xref=Rhea:RHEA:12044, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, CC ChEBI:CHEBI:17855, ChEBI:CHEBI:18035, ChEBI:CHEBI:28868; EC=3.1.1.79; CC Evidence={ECO:0000256|ARBA:ARBA00000803}; CC -!- PATHWAY: Glycerolipid metabolism; triacylglycerol degradation. CC {ECO:0000256|ARBA:ARBA00004879}. CC -!- PATHWAY: Lipid metabolism. {ECO:0000256|ARBA:ARBA00005189}. CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000256|ARBA:ARBA00004236}. CC Cytoplasm, cytosol {ECO:0000256|ARBA:ARBA00004514}. Membrane, caveola CC {ECO:0000256|ARBA:ARBA00004345}. CC -!- SIMILARITY: Belongs to the 'GDXG' lipolytic enzyme family. CC {ECO:0000256|ARBA:ARBA00010515}. CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ CC whole genome shotgun (WGS) entry which is preliminary data. CC {ECO:0000313|EMBL:RUS86042.1}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; RQTK01000152; RUS86042.1; -; Genomic_DNA. DR UniPathway; UPA00256; -. DR Proteomes; UP000271974; Unassembled WGS sequence. DR GO; GO:0016298; F:lipase activity; IEA:InterPro. DR GO; GO:0008203; P:cholesterol metabolic process; IEA:InterPro. DR GO; GO:0016042; P:lipid catabolic process; IEA:InterPro. DR InterPro; IPR029058; AB_hydrolase. DR InterPro; IPR013094; AB_hydrolase_3. DR InterPro; IPR010468; HSL_N. DR InterPro; IPR002168; Lipase_GDXG_HIS_AS. DR Pfam; PF07859; Abhydrolase_3; 2. DR Pfam; PF06350; HSL_N; 1. DR SUPFAM; SSF53474; SSF53474; 1. DR PROSITE; PS01173; LIPASE_GDXG_HIS; 1. PE 3: Inferred from homology; KW Cell membrane {ECO:0000256|ARBA:ARBA00022475}; KW Cholesterol metabolism {ECO:0000256|ARBA:ARBA00022548}; KW Hydrolase {ECO:0000256|ARBA:ARBA00022801}; KW Lipid degradation {ECO:0000256|ARBA:ARBA00022963}; KW Lipid metabolism {ECO:0000256|ARBA:ARBA00023098}; KW Membrane {ECO:0000256|ARBA:ARBA00023136}; KW Reference proteome {ECO:0000313|Proteomes:UP000271974}; KW Steroid metabolism {ECO:0000256|ARBA:ARBA00023221}; KW Sterol metabolism {ECO:0000256|ARBA:ARBA00023166}. FT DOMAIN 19..325 FT /note="HSL_N" FT /evidence="ECO:0000259|Pfam:PF06350" FT DOMAIN 358..474 FT /note="Abhydrolase_3" FT /evidence="ECO:0000259|Pfam:PF07859" FT DOMAIN 574..639 FT /note="Abhydrolase_3" FT /evidence="ECO:0000259|Pfam:PF07859" FT NON_TER 663 FT /evidence="ECO:0000313|EMBL:RUS86042.1" SQ SEQUENCE 663 AA; 73702 MW; 7EDE65F96B8D9641 CRC64; MMEFDSTDNP GRFFFALSKD LKSIALSNIQ FFKSGKQSNH AKFHVLFRLL YEYLDIGIEP TYAELSKLVS EYDYSPEIKG NGYRSLLRVV QKCCLHLLQL CRYISTVRDS MLFRKKVYAK ELESYVSALG QLRAVLYYAL KLTQYCQRGH LFADEDQLNS DIAEALMIEV ESLSQEYFYG RCLGFQYCES MQRPVQAIGI VMASLSEGYL ESSQVMRVAT SVFNSGKYFL DPELRAKQMV KVTRTADIRF CQTFWNFPES PIMHQLPSFV CPMMEVNEML TLGPDAFELP SVDGGETVTI TPPCAHTGPG HVYTRLISAQ LRDGQVGSEY ICSYNDSLTK SVKSKGPVSA KPRVPGLVVH FHGGGFVAQS SKSHEVYLRD WAVSLDCPIL SVDYSLAPQA PFPRALEECF YAYAWAVQNC EQLGSTGEKI IVVGDSAGGN LAISTAMRAA SFGIRPPDGA VLVYPCTVVK YTPSPARLLS LMDPLLPVGL MTRCLAGEEV CGSSPTLATS SPTGWVGVSI IRTPPTPPVI SNICFYENDR NCQIIYLNLE EMPTSTFEVQ NFSSHSPLHL LRRAAVVKNP YMSPLMASDE MLAGLTRVSI IGCHLDPLLD DSVMMARRLR DLGVPVDLHL VDDLPHGFLN FALLSMEARQ AADLCGRKIR DML //