ID A0A3S0ZT07_ELYCH Unreviewed; 663 AA. AC A0A3S0ZT07; DT 10-APR-2019, integrated into UniProtKB/TrEMBL. DT 10-APR-2019, sequence version 1. DT 25-MAY-2022, entry version 15. DE RecName: Full=Hormone-sensitive lipase {ECO:0000256|ARBA:ARBA00015845}; DE EC=3.1.1.23 {ECO:0000256|ARBA:ARBA00013254}; DE EC=3.1.1.79 {ECO:0000256|ARBA:ARBA00013088}; DE AltName: Full=Monoacylglycerol lipase LIPE {ECO:0000256|ARBA:ARBA00031112}; DE AltName: Full=Retinyl ester hydrolase {ECO:0000256|ARBA:ARBA00030031}; DE Flags: Fragment; GN ORFNames=EGW08_006196 {ECO:0000313|EMBL:RUS86042.1}; OS Elysia chlorotica (Eastern emerald elysia) (Sea slug). OC Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Mollusca; Gastropoda; OC Heterobranchia; Euthyneura; Panpulmonata; Sacoglossa; Placobranchoidea; OC Plakobranchidae; Elysia. OX NCBI_TaxID=188477 {ECO:0000313|EMBL:RUS86042.1, ECO:0000313|Proteomes:UP000271974}; RN [1] {ECO:0000313|EMBL:RUS86042.1, ECO:0000313|Proteomes:UP000271974} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=EC2010 {ECO:0000313|EMBL:RUS86042.1}; RC TISSUE=Whole organism of an adult {ECO:0000313|EMBL:RUS86042.1}; RA Cai H., Li Q., Fang X., Li J., Curtis N.E., Altenburger A., Shibata T., RA Feng M., Maeda T., Schwartz J.A., Shigenobu S., Lundholm N., Nishiyama T., RA Yang H., Hasebe M., Li S., Pierce S.K., Wang J.; RT "A draft genome assembly of the solar-powered sea slug Elysia chlorotica."; RL Submitted (JAN-2019) to the EMBL/GenBank/DDBJ databases. CC -!- CATALYTIC ACTIVITY: CC Reaction=(9Z)-octadecenoate + 1,2-di-(9Z-octadecenoyl)-glycerol + H(+) CC = 1,2,3-tri-(9Z-octadecenoyl)-glycerol + H2O; Xref=Rhea:RHEA:38379, CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30823, CC ChEBI:CHEBI:52323, ChEBI:CHEBI:53753; CC Evidence={ECO:0000256|ARBA:ARBA00023334}; CC PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:38381; CC Evidence={ECO:0000256|ARBA:ARBA00023334}; CC -!- CATALYTIC ACTIVITY: CC Reaction=1,2,3-tri-(9Z-octadecenoyl)-glycerol + H2O = (9Z)- CC octadecenoate + di-(9Z)-octadecenoylglycerol + H(+); CC Xref=Rhea:RHEA:38575, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, CC ChEBI:CHEBI:30823, ChEBI:CHEBI:53753, ChEBI:CHEBI:75945; CC Evidence={ECO:0000256|ARBA:ARBA00000652}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38576; CC Evidence={ECO:0000256|ARBA:ARBA00000652}; CC -!- CATALYTIC ACTIVITY: CC Reaction=1,2-di-(9Z-octadecenoyl)-glycerol + H2O = (9Z)-octadecenoate + CC (9Z-octadecenoyl)-glycerol + H(+); Xref=Rhea:RHEA:38455, CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30823, CC ChEBI:CHEBI:52323, ChEBI:CHEBI:75937; CC Evidence={ECO:0000256|ARBA:ARBA00023366}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38456; CC Evidence={ECO:0000256|ARBA:ARBA00023366}; CC -!- CATALYTIC ACTIVITY: CC Reaction=1,2-di-(9Z-octadecenoyl)-glycerol + H2O = (9Z)-octadecenoate + CC 2-(9Z-octadecenoyl)-glycerol + H(+); Xref=Rhea:RHEA:38659, CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30823, CC ChEBI:CHEBI:52323, ChEBI:CHEBI:73990; CC Evidence={ECO:0000256|ARBA:ARBA00023353}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38660; CC Evidence={ECO:0000256|ARBA:ARBA00023353}; CC -!- CATALYTIC ACTIVITY: CC Reaction=1,2-di-(9Z-octadecenoyl)-sn-glycerol + H2O = (9Z)- CC octadecenoate + (9Z-octadecenoyl)-glycerol + H(+); CC Xref=Rhea:RHEA:39935, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, CC ChEBI:CHEBI:30823, ChEBI:CHEBI:52333, ChEBI:CHEBI:75937; CC Evidence={ECO:0000256|ARBA:ARBA00023356}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:39936; CC Evidence={ECO:0000256|ARBA:ARBA00023356}; CC -!- CATALYTIC ACTIVITY: CC Reaction=1,3-di-(9Z-octadecenoyl)-glycerol + H2O = (9Z)-octadecenoate + CC (9Z-octadecenoyl)-glycerol + H(+); Xref=Rhea:RHEA:39939, CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30823, CC ChEBI:CHEBI:75735, ChEBI:CHEBI:75937; CC Evidence={ECO:0000256|ARBA:ARBA00023393}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:39940; CC Evidence={ECO:0000256|ARBA:ARBA00023393}; CC -!- CATALYTIC ACTIVITY: CC Reaction=1-(9Z-octadecenoyl)-glycerol + H2O = (9Z)-octadecenoate + CC glycerol + H(+); Xref=Rhea:RHEA:38487, ChEBI:CHEBI:15377, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:17754, ChEBI:CHEBI:30823, CC ChEBI:CHEBI:75342; Evidence={ECO:0000256|ARBA:ARBA00023407}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38488; CC Evidence={ECO:0000256|ARBA:ARBA00023407}; CC -!- CATALYTIC ACTIVITY: CC Reaction=1-O-hexadecyl-2-acetyl-sn-glycerol + H2O = 1-O-hexadecyl-sn- CC glycerol + acetate + H(+); Xref=Rhea:RHEA:38563, ChEBI:CHEBI:15377, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:30089, ChEBI:CHEBI:34115, CC ChEBI:CHEBI:75936; Evidence={ECO:0000256|ARBA:ARBA00023406}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38564; CC Evidence={ECO:0000256|ARBA:ARBA00023406}; CC -!- CATALYTIC ACTIVITY: CC Reaction=2,3-di-(9Z)-octadecenoyl-sn-glycerol + H2O = (9Z)- CC octadecenoate + 2-(9Z-octadecenoyl)-glycerol + H(+); CC Xref=Rhea:RHEA:38383, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, CC ChEBI:CHEBI:30823, ChEBI:CHEBI:73990, ChEBI:CHEBI:75824; CC Evidence={ECO:0000256|ARBA:ARBA00023360}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38384; CC Evidence={ECO:0000256|ARBA:ARBA00023360}; CC -!- CATALYTIC ACTIVITY: CC Reaction=2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-glycerol + H2O = CC (5Z,8Z,11Z,14Z)-eicosatetraenoate + glycerol + H(+); CC Xref=Rhea:RHEA:26132, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, CC ChEBI:CHEBI:17754, ChEBI:CHEBI:32395, ChEBI:CHEBI:52392; CC Evidence={ECO:0000256|ARBA:ARBA00000361}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:26133; CC Evidence={ECO:0000256|ARBA:ARBA00000361}; CC -!- CATALYTIC ACTIVITY: CC Reaction=2-(9Z-octadecenoyl)-glycerol + H2O = (9Z)-octadecenoate + CC glycerol + H(+); Xref=Rhea:RHEA:38491, ChEBI:CHEBI:15377, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:17754, ChEBI:CHEBI:30823, CC ChEBI:CHEBI:73990; Evidence={ECO:0000256|ARBA:ARBA00023382}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38492; CC Evidence={ECO:0000256|ARBA:ARBA00023382}; CC -!- CATALYTIC ACTIVITY: CC Reaction=Hydrolyzes glycerol monoesters of long-chain fatty acids.; CC EC=3.1.1.23; Evidence={ECO:0000256|ARBA:ARBA00001613}; CC -!- CATALYTIC ACTIVITY: CC Reaction=a diacylglycerol + H2O = a fatty acid + a monoacylglycerol + CC H(+); Xref=Rhea:RHEA:32731, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, CC ChEBI:CHEBI:17408, ChEBI:CHEBI:18035, ChEBI:CHEBI:28868; EC=3.1.1.79; CC Evidence={ECO:0000256|ARBA:ARBA00000354}; CC -!- CATALYTIC ACTIVITY: CC Reaction=a monoacylglycerol + H2O = a fatty acid + glycerol + H(+); CC Xref=Rhea:RHEA:15245, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, CC ChEBI:CHEBI:17408, ChEBI:CHEBI:17754, ChEBI:CHEBI:28868; EC=3.1.1.79; CC Evidence={ECO:0000256|ARBA:ARBA00001413}; CC -!- CATALYTIC ACTIVITY: CC Reaction=a triacylglycerol + H2O = a diacylglycerol + a fatty acid + CC H(+); Xref=Rhea:RHEA:12044, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, CC ChEBI:CHEBI:17855, ChEBI:CHEBI:18035, ChEBI:CHEBI:28868; EC=3.1.1.79; CC Evidence={ECO:0000256|ARBA:ARBA00000803}; CC -!- CATALYTIC ACTIVITY: CC Reaction=all-trans-retinyl hexadecanoate + H2O = all-trans-retinol + CC H(+) + hexadecanoate; Xref=Rhea:RHEA:13933, ChEBI:CHEBI:7896, CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17336, CC ChEBI:CHEBI:17616; Evidence={ECO:0000256|ARBA:ARBA00023384}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:13934; CC Evidence={ECO:0000256|ARBA:ARBA00023384}; CC -!- CATALYTIC ACTIVITY: CC Reaction=cholesteryl (9Z-octadecenoate) + H2O = (9Z)-octadecenoate + CC cholesterol + H(+); Xref=Rhea:RHEA:33875, ChEBI:CHEBI:15377, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:16113, ChEBI:CHEBI:30823, CC ChEBI:CHEBI:46898; Evidence={ECO:0000256|ARBA:ARBA00023350}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:33876; CC Evidence={ECO:0000256|ARBA:ARBA00023350}; CC -!- PATHWAY: Glycerolipid metabolism; triacylglycerol degradation. CC {ECO:0000256|ARBA:ARBA00004879}. CC -!- PATHWAY: Lipid metabolism. {ECO:0000256|ARBA:ARBA00005189}. CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000256|ARBA:ARBA00004236}. CC Cytoplasm, cytosol {ECO:0000256|ARBA:ARBA00004514}. Lipid droplet CC {ECO:0000256|ARBA:ARBA00004502}. Membrane, caveola CC {ECO:0000256|ARBA:ARBA00004345}. CC -!- SIMILARITY: Belongs to the 'GDXG' lipolytic enzyme family. CC {ECO:0000256|ARBA:ARBA00010515}. CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ CC whole genome shotgun (WGS) entry which is preliminary data. CC {ECO:0000313|EMBL:RUS86042.1}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; RQTK01000152; RUS86042.1; -; Genomic_DNA. DR STRING; 188477.A0A3S0ZT07; -. DR UniPathway; UPA00256; -. DR Proteomes; UP000271974; Unassembled WGS sequence. DR GO; GO:0005901; C:caveola; IEA:UniProtKB-SubCell. DR GO; GO:0005829; C:cytosol; IEA:UniProtKB-SubCell. DR GO; GO:0005811; C:lipid droplet; IEA:UniProtKB-SubCell. DR GO; GO:0047372; F:acylglycerol lipase activity; IEA:UniProtKB-EC. DR GO; GO:0047376; F:all-trans-retinyl-palmitate hydrolase, all-trans-retinol forming activity; IEA:RHEA. DR GO; GO:0033878; F:hormone-sensitive lipase activity; IEA:UniProtKB-EC. DR GO; GO:0004806; F:triglyceride lipase activity; IEA:RHEA. DR GO; GO:0008203; P:cholesterol metabolic process; IEA:UniProtKB-KW. DR GO; GO:0019433; P:triglyceride catabolic process; IEA:UniProtKB-UniPathway. DR Gene3D; 3.40.50.1820; -; 1. DR InterPro; IPR029058; AB_hydrolase. DR InterPro; IPR013094; AB_hydrolase_3. DR InterPro; IPR010468; HSL_N. DR InterPro; IPR002168; Lipase_GDXG_HIS_AS. DR Pfam; PF07859; Abhydrolase_3; 2. DR Pfam; PF06350; HSL_N; 1. DR SUPFAM; SSF53474; SSF53474; 1. DR PROSITE; PS01173; LIPASE_GDXG_HIS; 1. PE 3: Inferred from homology; KW Cell membrane {ECO:0000256|ARBA:ARBA00022475}; KW Cholesterol metabolism {ECO:0000256|ARBA:ARBA00022548}; KW Hydrolase {ECO:0000256|ARBA:ARBA00022801}; KW Lipid degradation {ECO:0000256|ARBA:ARBA00022963}; KW Lipid droplet {ECO:0000256|ARBA:ARBA00022677}; KW Lipid metabolism {ECO:0000256|ARBA:ARBA00023098}; KW Membrane {ECO:0000256|ARBA:ARBA00023136}; KW Reference proteome {ECO:0000313|Proteomes:UP000271974}; KW Steroid metabolism {ECO:0000256|ARBA:ARBA00023221}; KW Sterol metabolism {ECO:0000256|ARBA:ARBA00023166}. FT DOMAIN 19..325 FT /note="HSL_N" FT /evidence="ECO:0000259|Pfam:PF06350" FT DOMAIN 358..475 FT /note="Abhydrolase_3" FT /evidence="ECO:0000259|Pfam:PF07859" FT DOMAIN 574..639 FT /note="Abhydrolase_3" FT /evidence="ECO:0000259|Pfam:PF07859" FT NON_TER 663 FT /evidence="ECO:0000313|EMBL:RUS86042.1" SQ SEQUENCE 663 AA; 73702 MW; 7EDE65F96B8D9641 CRC64; MMEFDSTDNP GRFFFALSKD LKSIALSNIQ FFKSGKQSNH AKFHVLFRLL YEYLDIGIEP TYAELSKLVS EYDYSPEIKG NGYRSLLRVV QKCCLHLLQL CRYISTVRDS MLFRKKVYAK ELESYVSALG QLRAVLYYAL KLTQYCQRGH LFADEDQLNS DIAEALMIEV ESLSQEYFYG RCLGFQYCES MQRPVQAIGI VMASLSEGYL ESSQVMRVAT SVFNSGKYFL DPELRAKQMV KVTRTADIRF CQTFWNFPES PIMHQLPSFV CPMMEVNEML TLGPDAFELP SVDGGETVTI TPPCAHTGPG HVYTRLISAQ LRDGQVGSEY ICSYNDSLTK SVKSKGPVSA KPRVPGLVVH FHGGGFVAQS SKSHEVYLRD WAVSLDCPIL SVDYSLAPQA PFPRALEECF YAYAWAVQNC EQLGSTGEKI IVVGDSAGGN LAISTAMRAA SFGIRPPDGA VLVYPCTVVK YTPSPARLLS LMDPLLPVGL MTRCLAGEEV CGSSPTLATS SPTGWVGVSI IRTPPTPPVI SNICFYENDR NCQIIYLNLE EMPTSTFEVQ NFSSHSPLHL LRRAAVVKNP YMSPLMASDE MLAGLTRVSI IGCHLDPLLD DSVMMARRLR DLGVPVDLHL VDDLPHGFLN FALLSMEARQ AADLCGRKIR DML //