ID A0A3N0AE55_9ACTN Unreviewed; 454 AA. AC A0A3N0AE55; DT 13-FEB-2019, integrated into UniProtKB/TrEMBL. DT 13-FEB-2019, sequence version 1. DT 10-APR-2019, entry version 2. DE RecName: Full=Pyridinium-3,5-bisthiocarboxylic acid mononucleotide nickel insertion protein {ECO:0000256|HAMAP-Rule:MF_01074}; DE Short=P2TMN nickel insertion protein {ECO:0000256|HAMAP-Rule:MF_01074}; DE EC=4.99.1.12 {ECO:0000256|HAMAP-Rule:MF_01074}; DE AltName: Full=Nickel-pincer cofactor biosynthesis protein LarC {ECO:0000256|HAMAP-Rule:MF_01074}; GN Name=larC {ECO:0000256|HAMAP-Rule:MF_01074, GN ECO:0000313|EMBL:RNL19452.1}; GN ORFNames=DMP07_07060 {ECO:0000313|EMBL:RNL19452.1}; OS Slackia faecicanis. OC Bacteria; Actinobacteria; Coriobacteriia; Eggerthellales; OC Eggerthellaceae; Slackia. OX NCBI_TaxID=255723 {ECO:0000313|EMBL:RNL19452.1}; RN [1] {ECO:0000313|EMBL:RNL19452.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=DSM 17537 {ECO:0000313|EMBL:RNL19452.1}; RA Danylec N., Stoll D.A., Doetsch A., Huch M.; RT "Genome Sequencing of selected type strains of the family RT Eggerthellaceae."; RL Submitted (MAY-2018) to the EMBL/GenBank/DDBJ databases. CC -!- FUNCTION: Involved in the biosynthesis of a nickel-pincer cofactor CC ((SCS)Ni(II) pincer complex). Binds Ni(2+), and functions in CC nickel delivery to pyridinium-3,5-bisthiocarboxylic acid CC mononucleotide (P2TMN), to form the mature cofactor. Is thus CC probably required for the activation of nickel-pincer cofactor- CC dependent enzymes. {ECO:0000256|HAMAP-Rule:MF_01074}. CC -!- CATALYTIC ACTIVITY: CC Reaction=Ni(II)-pyridinium-3,5-bisthiocarboxylate mononucleotide = CC Ni(2+) + pyridinium-3,5-bisthiocarboxylate mononucleotide; CC Xref=Rhea:RHEA:54784, ChEBI:CHEBI:49786, ChEBI:CHEBI:137372, CC ChEBI:CHEBI:137373; EC=4.99.1.12; Evidence={ECO:0000256|HAMAP- CC Rule:MF_01074}; CC -!- SIMILARITY: Belongs to the LarC family. {ECO:0000256|HAMAP- CC Rule:MF_01074}. CC -!- CAUTION: The sequence shown here is derived from an CC EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is CC preliminary data. {ECO:0000313|EMBL:RNL19452.1}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; QICB01000005; RNL19452.1; -; Genomic_DNA. DR HAMAP; MF_01074; LarC; 1. DR InterPro; IPR002822; Ni_insertion. DR PANTHER; PTHR36566; PTHR36566; 1. DR Pfam; PF01969; DUF111; 1. DR TIGRFAMs; TIGR00299; TIGR00299; 1. PE 3: Inferred from homology; KW Lyase {ECO:0000256|HAMAP-Rule:MF_01074}; KW Nickel {ECO:0000256|HAMAP-Rule:MF_01074}. SQ SEQUENCE 454 AA; 48970 MW; EF77C0C5A99412CD CRC64; MGTMLYLECA AGISGDMTVA ALLDLGAREA VMCTALESLP VEGFDVRVSR VKKAGLDACD FAVLLDEAHE NRDHDMEYLH GPAPACDDFH DHDHDHDHDH DHDHDHDHDH DHDHDHDHDH DHAHRGLPEI DAIIDAAAMS ERAKAVAHRI FAILAQAEAQ AHGVPADQVH FHEVGAVDSI VDIVAAAVCL DDLDVDGVVV RGLTEGRGTV RCQHGVLPVP VPAVANIAAQ HAIPLSIADV QGELVTPTGA AIAAAVRTAS ELPERFIVRK TGIGAGKRSY AVPSMLRAML IEDAGSCASA ADASTPHGGP FVWKLETEVD DCPGEALGYA MERLFAAGAR EAHYAPVFMK KNRPAYQVQA LCAEEDIAAL EQVLFEETTT IGIRRTPMER TVLPREIVQV STTFGPMRAK CVAMPDGTRR LYPEYEDVAA AARAQELPYQ DVYRAALEAC AKDA //