ID A0A3G6V9Y8_9RHAB Unreviewed; 2066 AA. AC A0A3G6V9Y8; DT 13-FEB-2019, integrated into UniProtKB/TrEMBL. DT 13-FEB-2019, sequence version 1. DT 07-APR-2021, entry version 11. DE RecName: Full=Large structural protein {ECO:0000256|ARBA:ARBA00020375}; DE EC=2.7.7.48 {ECO:0000256|ARBA:ARBA00012494}; DE AltName: Full=Replicase {ECO:0000256|ARBA:ARBA00017192}; DE AltName: Full=Transcriptase {ECO:0000256|ARBA:ARBA00013861}; OS Rice stripe mosaic virus. OC Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; OC Monjiviricetes; Mononegavirales; Rhabdoviridae; Cytorhabdovirus; OC Rice stripe mosaic cytorhabdovirus. OX NCBI_TaxID=1931356 {ECO:0000313|EMBL:AZB50417.1}; RN [1] {ECO:0000313|EMBL:AZB50417.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=TP1 {ECO:0000313|EMBL:AZB50417.1}; RA Yang X., Zhou G.; RL Submitted (AUG-2018) to the EMBL/GenBank/DDBJ databases. CC -!- CATALYTIC ACTIVITY: CC Reaction=GTP + H2O = GDP + H(+) + phosphate; Xref=Rhea:RHEA:19669, CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:37565, CC ChEBI:CHEBI:43474, ChEBI:CHEBI:58189; CC Evidence={ECO:0000256|ARBA:ARBA00001270}; CC -!- CATALYTIC ACTIVITY: CC Reaction=a 5'-end (5'-triphosphoguanosine)-(2'-O-methyladenylyl)- CC adenylyl-cytidyl-adenosine in mRNA + S-adenosyl-L-methionine = a 5'- CC end (N(7)-methyl 5'-triphosphoguanosine)-(2'-O-methyladenylyl)- CC adenylyl-cytidyl-adenosine in mRNA + S-adenosyl-L-homocysteine; CC Xref=Rhea:RHEA:65440, Rhea:RHEA-COMP:16798, Rhea:RHEA-COMP:16801, CC ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:156482, CC ChEBI:CHEBI:156483; Evidence={ECO:0000256|ARBA:ARBA00023924}; CC -!- CATALYTIC ACTIVITY: CC Reaction=a 5'-end (5'-triphosphoguanosine)-adenylyl-adenylyl-cytidyl- CC adenosine in mRNA + 2 S-adenosyl-L-methionine = a 5'-end (N(7)-methyl CC 5'-triphosphoguanosine)-(2'-O-methyladenylyl)-adenylyl-cytidyl- CC adenosine in mRNA + H(+) + 2 S-adenosyl-L-homocysteine; CC Xref=Rhea:RHEA:65376, Rhea:RHEA-COMP:16797, Rhea:RHEA-COMP:16798, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, CC ChEBI:CHEBI:156483, ChEBI:CHEBI:156484; CC Evidence={ECO:0000256|ARBA:ARBA00023946}; CC -!- CATALYTIC ACTIVITY: CC Reaction=a 5'-end (5'-triphosphoguanosine)-adenylyl-adenylyl-cytidyl- CC adenosine in mRNA + S-adenosyl-L-methionine = a 5'-end (5'- CC triphosphoguanosine)-(2'-O-methyladenylyl)-adenylyl-cytidyl-adenosine CC in mRNA + H(+) + S-adenosyl-L-homocysteine; Xref=Rhea:RHEA:65380, CC Rhea:RHEA-COMP:16797, Rhea:RHEA-COMP:16801, ChEBI:CHEBI:15378, CC ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:156482, CC ChEBI:CHEBI:156484; Evidence={ECO:0000256|ARBA:ARBA00023913}; CC -!- SUBCELLULAR LOCATION: Virion {ECO:0000256|ARBA:ARBA00004328}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; MH720464; AZB50417.1; -; Viral_cRNA. DR GO; GO:0019012; C:virion; IEA:UniProtKB-SubCell. DR GO; GO:0005524; F:ATP binding; IEA:InterPro. DR GO; GO:0004482; F:mRNA (guanine-N7-)-methyltransferase activity; IEA:UniProtKB-EC. DR GO; GO:0003968; F:RNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-EC. DR InterPro; IPR026890; Mononeg_mRNAcap. DR InterPro; IPR014023; Mononeg_RNA_pol_cat. DR Pfam; PF14318; Mononeg_mRNAcap; 1. DR Pfam; PF00946; Mononeg_RNA_pol; 1. DR PROSITE; PS50526; RDRP_SSRNA_NEG_NONSEG; 1. PE 4: Predicted; KW mRNA capping {ECO:0000256|ARBA:ARBA00023042}; KW mRNA processing {ECO:0000256|ARBA:ARBA00022664}; KW Multifunctional enzyme {ECO:0000256|ARBA:ARBA00023268}; KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691}; KW Virion {ECO:0000256|ARBA:ARBA00022844}. FT DOMAIN 566..755 FT /note="RdRp catalytic" FT /evidence="ECO:0000259|PROSITE:PS50526" SQ SEQUENCE 2066 AA; 235884 MW; F2B949A02D61B328 CRC64; MDLDDGGLWR RARGLGDYHL RSALVTPSLE RFRSRKGRHR EQLCFDRMKS LGWMLRWVDQ GKLLGYLMVE ANKSLPKSIA NQELLVETLK LEYGCLRQII MTDGDLHDQV ISYLDRKSIS THYTHGREVF QEALIVVMAL SSGREPPDHV NNLGCEMLNE ELEVPVVRTY GVIFYLFGDL IYVKYPEEEG MITLDMFRNL TDKFSERENI MIATQLGTEI LQEIYPSETV LKTVFSLWDK GLLKEGNDFY TVVKTFEAII NGMLIKNNDG TYYDPSAYLR ETIMGLPVRL RDYAQTLVSY LDSLPFNPHH LSQIGGLFRL WGHPIVDPNA GVRKVRLLGT ADKMNLTHIP TLAERKFKEI FYLSYYEKHR VYPNHNLNGE IEGSYLLSQL AQMALVNPKH ANYSLVDWDS VNTLETFPFP KSFNLSLIIA DKAVSPNREE WLELRRKGGT QMDPHIRRGP LKAMKDGVID CEKLLRKIDR NPSGLAKKHR IIGLYPKERE ENMVPRMFAL MSFDMRAFSV VSESMIADHI IPHIEGVTMT KSMLALQKEM IISTKSQASS TQSDSITFCL NIDFEKWNLN FRRWMTEGVF REMGRLFGLP EIFNRTYDIF KKSIIYLADG SFDLLLTDEL EIEPGTNPDC AYTGHVGGFE GLRQKGWTVF TAVLISSICD EMGIKTHLMG QGDNQVLMLT IYSRAARETG DLKSAPAVLE ITNTLETFKS RLVSLFANLG LPIKPLETWV SEELFAYGKT PIYRAVPLAM SLKRISRVFA FSNEDLMTLS NALGAISANA QAASMCDVHP MVSYAIAKWQ HLCCAIIFSN YHPLCGCAPH VSGEEWAIKL RLPSGKKIQE TSDEEIDERD LMKLIVTIPR SLGGYNTLTL YEMIMRGFSD PVSRDMCWLF AIASESTGKL RGYLINWIKP IVSPEVNAQH LIQDPTALNL LVPPNATSVI KRMIDKSLEA LPKRSQFAVW FSEILEISGD KEISKLAEAL TRTDSLNPRF LHDILGATLY GYCTAITSKV DKTVTLSRMA LASKDVVGAL IKGEMRLYSY FGWRTLQSRG LPLTTRCPNK WVRIIRDISW QKQIKAVSVP YPTHFLSEDI SETDRPDSWI ECYIDDAPTS DRSCLIYSTG KALPYLGSVT REKLTTRGAK AAYGTEPLVL RPINLVRTIG WFIEEDSNFA ELIKMLLGAV TDLPIEEVLY IPEMVSGSMA HRYLDMSTQH GSLWMPLYGP ATFLHMSTNT FVQYLKGTEN VTLHFQCVMG LIQYAIVNKA LGECPTKRMT RFFRSCPDCI VPIDDTLEDL PEVPSLDLIP ERTTNPYLYL KKEKIELNVR HRLAVINEIR VIARDEIEET PLLANNTLED VMSLRAAQRI FYTAKGKEAQ WDLQTADREG YLKLDFVSVI RKIIGHLFVM ESETLKAGSD YPTFQLQQRR IMRRVRQSDT SNFVHLGGFF CWQESIARIQ KLKWSVMPAT FPITAESVSL AAKMSLIGAM TSGLTPKRCD GVLAENLIPD VTRQAKNIIC LDKVFQSRCD YCYTAAMTNR WSDSINSDSI FNMRCERGHT ILSPRMLHQL RRTILPEGAL YTLAVRVVHQ SPPEASQPLQ IIPCRRERYQ ILSEADMPRQ TGYSGVYRRP TNQICMYTEV ELAKKYRLPT NSLYRILDLH DCFFSQKLMD TRGNILVVGD GYGYSSLLTK CLNPDRNVVS WTYIEPSEAL PHSLRISKPP MHYKADVQID SSPSIDRISD IHNSSYPDEF AKVVTKNWIT ALISDIETVY VSGEKSVSSL INLAWNNQIQ LGALKLELMT DPLEKVVEYA HNAYQRWELF TLPGANLGGG VLYIGFYGRR EKLTGYIIPH SGVETLMDRL ATEVDEDRGR LRAEDRDRWE QLDTMESKIH LQQYHKMRLD LWFGSAYLSG YLSEDMTEFF YSVKTSYRPP AISWEKGNQA RYLYGSREEI LFMSMITVAL SSYLEDQDVL REFLSSSGWK LQWKKDKKNP RHWSPYLERS DSQLVWKKNK AMIFKLVSVL RGSKPREEYK MGKASDSIVF RYIPRKVREG EVPLCFPISK VASMSV //