ID A0A3G2QE10_9VIBR Unreviewed; 453 AA. AC A0A3G2QE10; DT 13-FEB-2019, integrated into UniProtKB/TrEMBL. DT 13-FEB-2019, sequence version 1. DT 10-APR-2019, entry version 2. DE RecName: Full=tRNA modification GTPase MnmE {ECO:0000256|HAMAP-Rule:MF_00379}; DE EC=3.6.-.- {ECO:0000256|HAMAP-Rule:MF_00379}; GN Name=mnmE {ECO:0000256|HAMAP-Rule:MF_00379, GN ECO:0000313|EMBL:AYO16115.1}; GN Synonyms=trmE {ECO:0000256|HAMAP-Rule:MF_00379}; GN ORFNames=D0812_17620 {ECO:0000313|EMBL:AYO16115.1}; OS Vibrio owensii. OC Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; OC Vibrionaceae; Vibrio. OX NCBI_TaxID=696485 {ECO:0000313|EMBL:AYO16115.1, ECO:0000313|Proteomes:UP000272136}; RN [1] {ECO:0000313|EMBL:AYO16115.1, ECO:0000313|Proteomes:UP000272136} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=1700302 {ECO:0000313|EMBL:AYO16115.1, RC ECO:0000313|Proteomes:UP000272136}; RA Yan M., Wang X., Wang Y.; RT "Whole Genome of Vibrio owensii strain 170502, isolated from Acute RT Hepatopancreatic Necrosis Disease (AHPND) shrimp."; RL Submitted (OCT-2018) to the EMBL/GenBank/DDBJ databases. CC -!- FUNCTION: Exhibits a very high intrinsic GTPase hydrolysis rate. CC Involved in the addition of a carboxymethylaminomethyl (cmnm) CC group at the wobble position (U34) of certain tRNAs, forming tRNA- CC cmnm(5)s(2)U34. {ECO:0000256|HAMAP-Rule:MF_00379}. CC -!- COFACTOR: CC Name=K(+); Xref=ChEBI:CHEBI:29103; Evidence={ECO:0000256|HAMAP- CC Rule:MF_00379}; CC Note=Binds 1 potassium ion per subunit. {ECO:0000256|HAMAP- CC Rule:MF_00379}; CC -!- SUBUNIT: Homodimer. Heterotetramer of two MnmE and two MnmG CC subunits. {ECO:0000256|HAMAP-Rule:MF_00379}. CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00379}. CC -!- SIMILARITY: Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin- CC like GTPase superfamily. TrmE GTPase family. {ECO:0000256|HAMAP- CC Rule:MF_00379, ECO:0000256|RuleBase:RU003313, CC ECO:0000256|SAAS:SAAS00534589}. CC -!- CAUTION: Lacks conserved residue(s) required for the propagation CC of feature annotation. {ECO:0000256|HAMAP-Rule:MF_00379}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; CP033137; AYO16115.1; -; Genomic_DNA. DR Proteomes; UP000272136; Chromosome 1. DR CDD; cd04164; trmE; 1. DR Gene3D; 1.20.120.430; -; 1. DR Gene3D; 3.30.1360.120; -; 1. DR HAMAP; MF_00379; GTPase_MnmE; 1. DR InterPro; IPR031168; G_TrmE. DR InterPro; IPR018948; GTP-bd_TrmE_N. DR InterPro; IPR006073; GTP_binding_domain. DR InterPro; IPR004520; GTPase_MnmE. DR InterPro; IPR027368; MnmE_dom2. DR InterPro; IPR025867; MnmE_helical. DR InterPro; IPR027417; P-loop_NTPase. DR InterPro; IPR005225; Small_GTP-bd_dom. DR InterPro; IPR027266; TrmE/GcvT_dom1. DR Pfam; PF01926; MMR_HSR1; 1. DR Pfam; PF12631; MnmE_helical; 1. DR Pfam; PF10396; TrmE_N; 1. DR SUPFAM; SSF52540; SSF52540; 1. DR TIGRFAMs; TIGR00450; mnmE_trmE_thdF; 1. DR TIGRFAMs; TIGR00231; small_GTP; 1. DR PROSITE; PS51709; G_TRME; 1. PE 3: Inferred from homology; KW Complete proteome {ECO:0000313|Proteomes:UP000272136}; KW Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00379}; KW GTP-binding {ECO:0000256|HAMAP-Rule:MF_00379, KW ECO:0000256|RuleBase:RU003313, ECO:0000256|SAAS:SAAS00415297}; KW Hydrolase {ECO:0000256|HAMAP-Rule:MF_00379}; KW Magnesium {ECO:0000256|HAMAP-Rule:MF_00379}; KW Metal-binding {ECO:0000256|HAMAP-Rule:MF_00379}; KW Nucleotide-binding {ECO:0000256|HAMAP-Rule:MF_00379, KW ECO:0000256|RuleBase:RU003313, ECO:0000256|SAAS:SAAS00027939}; KW Potassium {ECO:0000256|HAMAP-Rule:MF_00379}; KW tRNA processing {ECO:0000256|HAMAP-Rule:MF_00379, KW ECO:0000256|RuleBase:RU003313, ECO:0000256|SAAS:SAAS00027974}. FT DOMAIN 215 376 TrmE-type G. {ECO:0000259|PROSITE: FT PS51709}. FT NP_BIND 225 230 GTP. {ECO:0000256|HAMAP-Rule:MF_00379}. FT NP_BIND 244 250 GTP. {ECO:0000256|HAMAP-Rule:MF_00379}. FT NP_BIND 269 272 GTP. {ECO:0000256|HAMAP-Rule:MF_00379}. FT NP_BIND 334 337 GTP. {ECO:0000256|HAMAP-Rule:MF_00379}. FT METAL 225 225 Potassium. {ECO:0000256|HAMAP-Rule: FT MF_00379}. FT METAL 229 229 Magnesium. {ECO:0000256|HAMAP-Rule: FT MF_00379}. FT METAL 244 244 Potassium; via carbonyl oxygen. FT {ECO:0000256|HAMAP-Rule:MF_00379}. FT METAL 246 246 Potassium; via carbonyl oxygen. FT {ECO:0000256|HAMAP-Rule:MF_00379}. FT METAL 249 249 Potassium. {ECO:0000256|HAMAP-Rule: FT MF_00379}. FT METAL 250 250 Magnesium. {ECO:0000256|HAMAP-Rule: FT MF_00379}. FT BINDING 22 22 Formyltetrahydrofolate. FT {ECO:0000256|HAMAP-Rule:MF_00379}. FT BINDING 79 79 Formyltetrahydrofolate. FT {ECO:0000256|HAMAP-Rule:MF_00379}. FT BINDING 119 119 Formyltetrahydrofolate. FT {ECO:0000256|HAMAP-Rule:MF_00379}. FT BINDING 453 453 Formyltetrahydrofolate. FT {ECO:0000256|HAMAP-Rule:MF_00379}. SQ SEQUENCE 453 AA; 49214 MW; 9F66C5BD5169F460 CRC64; MTTDTIVAQA TAPGRGGVGI IRVSGPKANQ VALEVTGKTL KPRYAEYLPF QAEDGTVLDQ GIALYFPNPH SFTGEDVLEL QGHGGPVVMD MLIKRILGID GVRAARPGEF SERAFLNDKM DLTQAEAIAD LIDASSEEAA KSALQSLQGQ FSQRIQTLVE SLIHLRIYVE AAIDFPEEEI DFLADGKVSG DLQTIIDNLD AVRKEANQGA IMREGMKVVI AGRPNAGKSS LLNALSGKES AIVTDIAGTT RDVLREHIHI DGMPLHIIDT AGLRDASDEV EKIGIERAWD EIAQADRVLF MVDGTTTDAT DPKDIWPDFV DRLPNSIGMT VIRNKADQTG EDMGICHVND PTLIRLSAKT GAGVDALRTH LKECMGFSGN TEGGFMARRR HLDALERAAQ HLQIGQEQLE GYMAGEILAE ELRITQQHLN EITGEFSSDD LLGRIFSSFC IGK //