ID A0A3A3KD82_SALMO Unreviewed; 457 AA. AC A0A3A3KD82; DT 05-DEC-2018, integrated into UniProtKB/TrEMBL. DT 05-DEC-2018, sequence version 1. DT 03-MAY-2023, entry version 28. DE RecName: Full=Siroheme synthase {ECO:0000256|HAMAP-Rule:MF_01646}; DE Includes: DE RecName: Full=Uroporphyrinogen-III C-methyltransferase {ECO:0000256|HAMAP-Rule:MF_01646}; DE Short=Urogen III methylase {ECO:0000256|HAMAP-Rule:MF_01646}; DE EC=2.1.1.107 {ECO:0000256|HAMAP-Rule:MF_01646}; DE AltName: Full=SUMT {ECO:0000256|HAMAP-Rule:MF_01646}; DE AltName: Full=Uroporphyrinogen III methylase {ECO:0000256|HAMAP-Rule:MF_01646}; DE Short=UROM {ECO:0000256|HAMAP-Rule:MF_01646}; DE Includes: DE RecName: Full=Precorrin-2 dehydrogenase {ECO:0000256|HAMAP-Rule:MF_01646}; DE EC=1.3.1.76 {ECO:0000256|HAMAP-Rule:MF_01646}; DE Includes: DE RecName: Full=Sirohydrochlorin ferrochelatase {ECO:0000256|HAMAP-Rule:MF_01646}; DE EC=4.99.1.4 {ECO:0000256|HAMAP-Rule:MF_01646}; GN Name=cobA {ECO:0000313|EMBL:EBO8724379.1}; GN Synonyms=cysG {ECO:0000256|HAMAP-Rule:MF_01646, GN ECO:0000313|EMBL:EDI2489406.1}; GN ORFNames=AHY82_05955 {ECO:0000313|EMBL:ECV9755739.1}, AIG37_12450 GN {ECO:0000313|EMBL:ECZ7803670.1}, APO85_22565 GN {ECO:0000313|EMBL:ECX1556874.1}, AU259_12670 GN {ECO:0000313|EMBL:ECY3344894.1}, AVH73_20640 GN {ECO:0000313|EMBL:ECY9608729.1}, AXU14_19530 GN {ECO:0000313|EMBL:EDA0012506.1}, AXU32_12220 GN {ECO:0000313|EMBL:EDB4301317.1}, B0D32_12790 GN {ECO:0000313|EMBL:EDF3868613.1}, B5881_01010 GN {ECO:0000313|EMBL:EDG1349773.1}, B6C67_06015 GN {ECO:0000313|EMBL:EDG0896523.1}, B6F82_01695 GN {ECO:0000313|EMBL:QVS86478.1}, BIW02_13375 GN {ECO:0000313|EMBL:EDC6720060.1}, BS131_20695 GN {ECO:0000313|EMBL:EDI2489406.1}, BZ049_13945 GN {ECO:0000313|EMBL:EDF3746908.1}, C2689_15270 GN {ECO:0000313|EMBL:ECY6787821.1}, CW117_11520 GN {ECO:0000313|EMBL:ECS8999704.1}, D0215_03540 GN {ECO:0000313|EMBL:ECV0718804.1}, D2K49_02285 GN {ECO:0000313|EMBL:ECT3325251.1}, D9U82_00785 GN {ECO:0000313|EMBL:EBZ3293249.1}, DK664_00780 GN {ECO:0000313|EMBL:ECS9166961.1}, DPE74_06105 GN {ECO:0000313|EMBL:EBW3454518.1}, DPL19_03830 GN {ECO:0000313|EMBL:ECV3149336.1}, DQS63_22775 GN {ECO:0000313|EMBL:EBS4622864.1}, DT992_02860 GN {ECO:0000313|EMBL:ECV2332036.1}, DYO72_17690 GN {ECO:0000313|EMBL:ECU3335379.1}, DYT96_08350 GN {ECO:0000313|EMBL:ECU3685001.1}, E0U36_04570 GN {ECO:0000313|EMBL:ECB6706091.1}, E1406_10255 GN {ECO:0000313|EMBL:ECG5541193.1}, E4933_11310 GN {ECO:0000313|EMBL:KAA7153371.1}, E4S88_10635 GN {ECO:0000313|EMBL:EBO8724379.1}, EIC46_01300 GN {ECO:0000313|EMBL:EBO8585431.1}, FEH78_05450 GN {ECO:0000313|EMBL:EBM9175818.1}, FJJ53_08865 GN {ECO:0000313|EMBL:EBG8086904.1}, G2800_22205 GN {ECO:0000313|EMBL:HAE1156762.1}, G2805_22200 GN {ECO:0000313|EMBL:HAE0613076.1}, G2893_22775 GN {ECO:0000313|EMBL:HAE0809426.1}, G2898_19095 GN {ECO:0000313|EMBL:HAE0786146.1}, G2910_08000 GN {ECO:0000313|EMBL:HAE0880103.1}, G2911_15745 GN {ECO:0000313|EMBL:HAE0767951.1}, G3401_001371 GN {ECO:0000313|EMBL:HAE2971120.1}, G4B33_000853 GN {ECO:0000313|EMBL:HAE3985214.1}, G4Y05_003679 GN {ECO:0000313|EMBL:HAE9354060.1}, G4Y21_000153 GN {ECO:0000313|EMBL:HAE8947232.1}, G4Y22_002812 GN {ECO:0000313|EMBL:HAE9389018.1}, G9254_000510 GN {ECO:0000313|EMBL:HAF7388039.1}, G9G40_000203 GN {ECO:0000313|EMBL:HAF1250544.1}, G9G43_003152 GN {ECO:0000313|EMBL:HAF0923478.1}, G9X30_000953 GN {ECO:0000313|EMBL:HAF7251952.1}, GBH27_09500 GN {ECO:0000313|EMBL:EDF9740439.1}, GND10_002434 GN {ECO:0000313|EMBL:HAE4143879.1}, II32_05055 GN {ECO:0000313|EMBL:ECW6425007.1}; OS Salmonella montevideo. OC Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; OC Enterobacteriaceae; Salmonella. OX NCBI_TaxID=115981 {ECO:0000313|EMBL:EBO8724379.1}; RN [1] {ECO:0000313|EMBL:HAE0613076.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=09-0646 {ECO:0000313|EMBL:HAF7251952.1}, 09-1991 RC {ECO:0000313|EMBL:HAE2971120.1}, 09-2247 RC {ECO:0000313|EMBL:HAE9354060.1}, 10-2196 RC {ECO:0000313|EMBL:HAF7388039.1}, 10-6667 RC {ECO:0000313|EMBL:HAE9389018.1}, 10-6841 RC {ECO:0000313|EMBL:HAE3985214.1}, 12-1128 RC {ECO:0000313|EMBL:HAE8947232.1}, 12-4802 RC {ECO:0000313|EMBL:HAF1250544.1}, 13-0136 RC {ECO:0000313|EMBL:HAF0923478.1}, BCW_2023 RC {ECO:0000313|EMBL:HAE4143879.1}, and Salmonella enterica RC {ECO:0000313|EMBL:HAE0613076.1}; RX PubMed=30286803; RA Souvorov A., Agarwala R., Lipman D.J.; RT "SKESA: strategic k-mer extension for scrupulous assemblies."; RL Genome Biol. 19:153-150(2018). RN [2] {ECO:0000313|EMBL:EDF3746908.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=PNUSAS005717 {ECO:0000313|EMBL:EDI2489406.1}, PNUSAS007847 RC {ECO:0000313|EMBL:EDF3746908.1}, and PNUSAS010114 RC {ECO:0000313|EMBL:EDG1349773.1}; RG PulseNet: The National Subtyping Network for Foodborne Disease Surveillance; RA Tarr C.L., Trees E., Katz L.S., Carleton-Romer H.A., Stroika S., RA Kucerova Z., Roache K.F., Sabol A.L., Besser J., Gerner-Smidt P.; RL Submitted (JUL-2018) to the EMBL/GenBank/DDBJ databases. RN [3] {ECO:0000313|EMBL:ECU3335379.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=CVM N17S1479 {ECO:0000313|EMBL:ECU3335379.1}, FSIS11705537 RC {ECO:0000313|EMBL:ECS8999704.1}, FSIS11807106 RC {ECO:0000313|EMBL:ECY6787821.1}, FSIS11810643 RC {ECO:0000313|EMBL:ECV3149336.1}, FSIS11814638 RC {ECO:0000313|EMBL:EBZ3293249.1}, FSIS11815944 RC {ECO:0000313|EMBL:EBO8585431.1}, FSIS11920988 RC {ECO:0000313|EMBL:EBM9175818.1}, FSIS1500714 RC {ECO:0000313|EMBL:ECX1556874.1}, FSIS1504186 RC {ECO:0000313|EMBL:ECY3344894.1}, FSIS1607655 RC {ECO:0000313|EMBL:EDC6720060.1}, and FSIS1700054 RC {ECO:0000313|EMBL:EDG0896523.1}; RG NARMS: The National Antimicrobial Resistance Monitoring System; RL Submitted (AUG-2018) to the EMBL/GenBank/DDBJ databases. RN [4] {ECO:0000313|EMBL:KAA7153371.1, ECO:0000313|Proteomes:UP000322618} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=ME2L-19-263 {ECO:0000313|EMBL:KAA7153371.1, RC ECO:0000313|Proteomes:UP000322618}; RX PubMed=31527278; DOI=.1073/pnas.1907787116; RA Rudman S.M., Greenblum S., Hughes R.C., Rajpurohit S., Kiratli O., RA Lowder D.B., Lemmon S.G., Petrov D.A., Chaston J.M., Schmidt P.; RT "Microbiome composition shapes rapid genomic adaptation of Drosophila RT melanogaster."; RL Proc. Natl. Acad. Sci. U.S.A. 116:20025-20032(2019). RN [5] {ECO:0000313|EMBL:KAA7153371.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=ME2L-19-263 {ECO:0000313|EMBL:KAA7153371.1}; RA Levent G., Schlochtermeier A., Ives S.E., Norman K.N., Lawhon S.D., RA Loneragan G.H., Anderson R.C., Scott H.M.; RL Submitted (MAR-2019) to the EMBL/GenBank/DDBJ databases. RN [6] {ECO:0000313|EMBL:HAE0613076.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=09-0646 {ECO:0000313|EMBL:HAF7251952.1}, 09-1991 RC {ECO:0000313|EMBL:HAE2971120.1}, 09-2247 RC {ECO:0000313|EMBL:HAE9354060.1}, 10-2196 RC {ECO:0000313|EMBL:HAF7388039.1}, 10-6667 RC {ECO:0000313|EMBL:HAE9389018.1}, 10-6841 RC {ECO:0000313|EMBL:HAE3985214.1}, 12-1128 RC {ECO:0000313|EMBL:HAE8947232.1}, 12-4802 RC {ECO:0000313|EMBL:HAF1250544.1}, 13-0136 RC {ECO:0000313|EMBL:HAF0923478.1}, BCW_2023 RC {ECO:0000313|EMBL:HAE4143879.1}, and Salmonella enterica RC {ECO:0000313|EMBL:HAE0613076.1}; RG NCBI Pathogen Detection Project; RL Submitted (APR-2019) to the EMBL/GenBank/DDBJ databases. RN [7] {ECO:0000313|EMBL:EBO8724379.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=CFSAN008715 {ECO:0000313|EMBL:QVS86478.1}, CFSAN032143 RC {ECO:0000313|EMBL:EDA0012506.1}, CFSAN032161 RC {ECO:0000313|EMBL:EDB4301317.1}, FDA00001465 RC {ECO:0000313|EMBL:ECV9755739.1}, FDA00004064 RC {ECO:0000313|EMBL:ECZ7803670.1}, FSIS11809966 RC {ECO:0000313|EMBL:ECS9166961.1}, FSIS11811659 RC {ECO:0000313|EMBL:ECV2332036.1}, FSIS11812555 RC {ECO:0000313|EMBL:ECU3685001.1}, FSIS11813078 RC {ECO:0000313|EMBL:ECV0718804.1}, FSIS11813543 RC {ECO:0000313|EMBL:ECT3325251.1}, FSIS11919213 RC {ECO:0000313|EMBL:EBO8724379.1}, FSIS1709880 RC {ECO:0000313|EMBL:EDF3868613.1}, and MDH-2014-00368 RC {ECO:0000313|EMBL:ECW6425007.1}; RG GenomeTrakr network: Whole genome sequencing for foodborne pathogen traceback; RL Submitted (JUN-2019) to the EMBL/GenBank/DDBJ databases. RN [8] {ECO:0000313|EMBL:EBG8086904.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=160770 {ECO:0000313|EMBL:EBS4622864.1}, 197101 RC {ECO:0000313|EMBL:ECG5541193.1}, 319650 RC {ECO:0000313|EMBL:ECB6706091.1}, 401701 RC {ECO:0000313|EMBL:EBW3454518.1}, 56964 RC {ECO:0000313|EMBL:ECY9608729.1}, 758106 RC {ECO:0000313|EMBL:EBG8086904.1}, and 818883 RC {ECO:0000313|EMBL:EDF9740439.1}; RA Ashton P.M., Dallman T., Nair S., De Pinna E., Peters T., Grant K.; RL Submitted (JUN-2019) to the EMBL/GenBank/DDBJ databases. RN [9] {ECO:0000313|EMBL:QVS86478.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=CFSAN008715 {ECO:0000313|EMBL:QVS86478.1}; RA Hoffmann M., Balkey M., Luo Y.; RT "Whole genome PacBio Sequel sequence of Salmonella enterica subsp. RT enterica."; RL Submitted (MAY-2021) to the EMBL/GenBank/DDBJ databases. CC -!- FUNCTION: Multifunctional enzyme that catalyzes the SAM-dependent CC methylations of uroporphyrinogen III at position C-2 and C-7 to form CC precorrin-2 via precorrin-1. Then it catalyzes the NAD-dependent ring CC dehydrogenation of precorrin-2 to yield sirohydrochlorin. Finally, it CC catalyzes the ferrochelation of sirohydrochlorin to yield siroheme. CC {ECO:0000256|HAMAP-Rule:MF_01646}. CC -!- CATALYTIC ACTIVITY: CC Reaction=2 H(+) + siroheme = Fe(2+) + sirohydrochlorin; CC Xref=Rhea:RHEA:24360, ChEBI:CHEBI:15378, ChEBI:CHEBI:29033, CC ChEBI:CHEBI:58351, ChEBI:CHEBI:60052; EC=4.99.1.4; CC Evidence={ECO:0000256|HAMAP-Rule:MF_01646}; CC -!- CATALYTIC ACTIVITY: CC Reaction=2 S-adenosyl-L-methionine + uroporphyrinogen III = H(+) + CC precorrin-2 + 2 S-adenosyl-L-homocysteine; Xref=Rhea:RHEA:32459, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57308, ChEBI:CHEBI:57856, CC ChEBI:CHEBI:58827, ChEBI:CHEBI:59789; EC=2.1.1.107; CC Evidence={ECO:0000256|HAMAP-Rule:MF_01646}; CC -!- CATALYTIC ACTIVITY: CC Reaction=NAD(+) + precorrin-2 = 2 H(+) + NADH + sirohydrochlorin; CC Xref=Rhea:RHEA:15613, ChEBI:CHEBI:15378, ChEBI:CHEBI:57540, CC ChEBI:CHEBI:57945, ChEBI:CHEBI:58351, ChEBI:CHEBI:58827; EC=1.3.1.76; CC Evidence={ECO:0000256|ARBA:ARBA00001156, ECO:0000256|HAMAP- CC Rule:MF_01646}; CC -!- PATHWAY: Cofactor biosynthesis; adenosylcobalamin biosynthesis; CC precorrin-2 from uroporphyrinogen III: step 1/1. {ECO:0000256|HAMAP- CC Rule:MF_01646}. CC -!- PATHWAY: Cofactor biosynthesis; adenosylcobalamin biosynthesis; CC sirohydrochlorin from precorrin-2: step 1/1. {ECO:0000256|HAMAP- CC Rule:MF_01646}. CC -!- PATHWAY: Porphyrin-containing compound metabolism; siroheme CC biosynthesis; precorrin-2 from uroporphyrinogen III: step 1/1. CC {ECO:0000256|ARBA:ARBA00025705, ECO:0000256|HAMAP-Rule:MF_01646}. CC -!- PATHWAY: Porphyrin-containing compound metabolism; siroheme CC biosynthesis; siroheme from sirohydrochlorin: step 1/1. CC {ECO:0000256|HAMAP-Rule:MF_01646}. CC -!- PATHWAY: Porphyrin-containing compound metabolism; siroheme CC biosynthesis; sirohydrochlorin from precorrin-2: step 1/1. CC {ECO:0000256|ARBA:ARBA00005010, ECO:0000256|HAMAP-Rule:MF_01646}. CC -!- SIMILARITY: Belongs to the precorrin methyltransferase family. CC {ECO:0000256|ARBA:ARBA00005879, ECO:0000256|RuleBase:RU003960}. CC -!- SIMILARITY: In the C-terminal section; belongs to the precorrin CC methyltransferase family. {ECO:0000256|HAMAP-Rule:MF_01646}. CC -!- SIMILARITY: In the N-terminal section; belongs to the precorrin-2 CC dehydrogenase / sirohydrochlorin ferrochelatase family. CC {ECO:0000256|HAMAP-Rule:MF_01646}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AAFKBF010000006; EBG8086904.1; -; Genomic_DNA. DR EMBL; AAGEDJ010000003; EBM9175818.1; -; Genomic_DNA. DR EMBL; AAGJVW010000001; EBO8585431.1; -; Genomic_DNA. DR EMBL; AAGJXF010000008; EBO8724379.1; -; Genomic_DNA. DR EMBL; AAGVOP010000264; EBS4622864.1; -; Genomic_DNA. DR EMBL; AAHIBZ010000005; EBW3454518.1; -; Genomic_DNA. DR EMBL; AAHQUA010000001; EBZ3293249.1; -; Genomic_DNA. DR EMBL; AAHYIA010000002; ECB6706091.1; -; Genomic_DNA. DR EMBL; AAIOTV010000008; ECG5541193.1; -; Genomic_DNA. DR EMBL; AAKKUG010000004; ECS8999704.1; -; Genomic_DNA. DR EMBL; AAKLCW010000001; ECS9166961.1; -; Genomic_DNA. DR EMBL; AAKMKW010000002; ECT3325251.1; -; Genomic_DNA. DR EMBL; AAKPPR010000007; ECU3335379.1; -; Genomic_DNA. DR EMBL; AAKPRQ010000007; ECU3685001.1; -; Genomic_DNA. DR EMBL; AAKSUE010000003; ECV0718804.1; -; Genomic_DNA. DR EMBL; AAKSJB010000002; ECV2332036.1; -; Genomic_DNA. DR EMBL; AAKSCW010000003; ECV3149336.1; -; Genomic_DNA. DR EMBL; AAKUXP010000005; ECV9755739.1; -; Genomic_DNA. DR EMBL; AAKXFP010000003; ECW6425007.1; -; Genomic_DNA. DR EMBL; AAKYVF010000039; ECX1556874.1; -; Genomic_DNA. DR EMBL; AALCUX010000007; ECY3344894.1; -; Genomic_DNA. DR EMBL; AALEDO010000008; ECY6787821.1; -; Genomic_DNA. DR EMBL; AALGFK010000042; ECY9608729.1; -; Genomic_DNA. DR EMBL; AALHUF010000006; ECZ7803670.1; -; Genomic_DNA. DR EMBL; AALIOX010000018; EDA0012506.1; -; Genomic_DNA. DR EMBL; AALNLT010000029; EDB4301317.1; -; Genomic_DNA. DR EMBL; AALRRW010000008; EDC6720060.1; -; Genomic_DNA. DR EMBL; AAMAPH010000009; EDF3746908.1; -; Genomic_DNA. DR EMBL; AAMARA010000006; EDF3868613.1; -; Genomic_DNA. DR EMBL; AAMCON010000007; EDF9740439.1; -; Genomic_DNA. DR EMBL; AAMCZA010000004; EDG0896523.1; -; Genomic_DNA. DR EMBL; AAMDCD010000001; EDG1349773.1; -; Genomic_DNA. DR EMBL; AAMKGK010000033; EDI2489406.1; -; Genomic_DNA. DR EMBL; DAAQRW010000021; HAE0613076.1; -; Genomic_DNA. DR EMBL; DAAQTF010000007; HAE0767951.1; -; Genomic_DNA. DR EMBL; DAAQTK010000053; HAE0786146.1; -; Genomic_DNA. DR EMBL; DAAQTN010000024; HAE0809426.1; -; Genomic_DNA. DR EMBL; DAAQUD010000003; HAE0880103.1; -; Genomic_DNA. DR EMBL; DAAQWK010000020; HAE1156762.1; -; Genomic_DNA. DR EMBL; DAARLW010000003; HAE2971120.1; -; Genomic_DNA. DR EMBL; DAARUM010000003; HAE3985214.1; -; Genomic_DNA. DR EMBL; DAARVT010000008; HAE4143879.1; -; Genomic_DNA. DR EMBL; DAATOZ010000001; HAE8947232.1; -; Genomic_DNA. DR EMBL; DAATOC010000049; HAE9354060.1; -; Genomic_DNA. DR EMBL; DAATOK010000005; HAE9389018.1; -; Genomic_DNA. DR EMBL; DAAUAZ010000008; HAF0923478.1; -; Genomic_DNA. DR EMBL; DAAUCY010000001; HAF1250544.1; -; Genomic_DNA. DR EMBL; DAAWCN010000004; HAF7251952.1; -; Genomic_DNA. DR EMBL; DAAWDQ010000003; HAF7388039.1; -; Genomic_DNA. DR EMBL; SRBT01000004; KAA7153371.1; -; Genomic_DNA. DR EMBL; CP074666; QVS86478.1; -; Genomic_DNA. DR RefSeq; WP_000349904.1; NZ_VCUN02000005.1. DR AlphaFoldDB; A0A3A3KD82; -. DR UniPathway; UPA00148; UER00211. DR UniPathway; UPA00262; UER00211. DR Proteomes; UP000322618; Unassembled WGS sequence. DR Proteomes; UP000839901; Unassembled WGS sequence. DR GO; GO:0051287; F:NAD binding; IEA:InterPro. DR GO; GO:0043115; F:precorrin-2 dehydrogenase activity; IEA:UniProtKB-UniRule. DR GO; GO:0051266; F:sirohydrochlorin ferrochelatase activity; IEA:UniProtKB-EC. DR GO; GO:0004851; F:uroporphyrin-III C-methyltransferase activity; IEA:UniProtKB-UniRule. DR GO; GO:0009236; P:cobalamin biosynthetic process; IEA:UniProtKB-UniRule. DR GO; GO:0032259; P:methylation; IEA:UniProtKB-KW. DR GO; GO:0019354; P:siroheme biosynthetic process; IEA:UniProtKB-UniRule. DR CDD; cd11642; SUMT; 1. DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 1. DR Gene3D; 1.10.8.210; Sirohaem synthase, dimerisation domain; 1. DR HAMAP; MF_01646; Siroheme_synth; 1. DR InterPro; IPR000878; 4pyrrol_Mease. DR InterPro; IPR035996; 4pyrrol_Methylase_sf. DR InterPro; IPR014777; 4pyrrole_Mease_sub1. DR InterPro; IPR014776; 4pyrrole_Mease_sub2. DR InterPro; IPR006366; CobA/CysG_C. DR InterPro; IPR036291; NAD(P)-bd_dom_sf. DR InterPro; IPR037115; Sirohaem_synt_dimer_dom_sf. DR InterPro; IPR012409; Sirohaem_synth. DR InterPro; IPR028281; Sirohaem_synthase_central. DR InterPro; IPR019478; Sirohaem_synthase_dimer_dom. DR InterPro; IPR006367; Sirohaem_synthase_N. DR InterPro; IPR003043; Uropor_MeTrfase_CS. DR PANTHER; PTHR45790:SF1; SIROHEME SYNTHASE; 1. DR PANTHER; PTHR45790; SIROHEME SYNTHASE-RELATED; 1. DR Pfam; PF10414; CysG_dimeriser; 1. DR Pfam; PF13241; NAD_binding_7; 1. DR Pfam; PF14824; Sirohm_synth_M; 1. DR Pfam; PF00590; TP_methylase; 1. DR PIRSF; PIRSF036426; Sirohaem_synth; 1. DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 1. DR SUPFAM; SSF75615; Siroheme synthase middle domains-like; 1. DR SUPFAM; SSF53790; Tetrapyrrole methylase; 1. DR TIGRFAMs; TIGR01469; cobA_cysG_Cterm; 1. DR TIGRFAMs; TIGR01470; cysG_Nterm; 1. DR PROSITE; PS00839; SUMT_1; 1. DR PROSITE; PS00840; SUMT_2; 1. PE 3: Inferred from homology; KW Cobalamin biosynthesis {ECO:0000256|ARBA:ARBA00022573, ECO:0000256|HAMAP- KW Rule:MF_01646}; KW Lyase {ECO:0000256|ARBA:ARBA00023239, ECO:0000256|HAMAP-Rule:MF_01646}; KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603, ECO:0000256|HAMAP- KW Rule:MF_01646}; KW Multifunctional enzyme {ECO:0000256|ARBA:ARBA00023268, ECO:0000256|HAMAP- KW Rule:MF_01646}; KW NAD {ECO:0000256|ARBA:ARBA00023027, ECO:0000256|HAMAP-Rule:MF_01646}; KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002, ECO:0000256|HAMAP- KW Rule:MF_01646}; Phosphoprotein {ECO:0000256|HAMAP-Rule:MF_01646}; KW Porphyrin biosynthesis {ECO:0000256|ARBA:ARBA00023244, ECO:0000256|HAMAP- KW Rule:MF_01646}; KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691, ECO:0000256|HAMAP- KW Rule:MF_01646}; KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|HAMAP- KW Rule:MF_01646}. FT DOMAIN 119..145 FT /note="Siroheme synthase central" FT /evidence="ECO:0000259|Pfam:PF14824" FT DOMAIN 150..207 FT /note="Sirohaem synthase dimerisation" FT /evidence="ECO:0000259|Pfam:PF10414" FT DOMAIN 218..426 FT /note="Tetrapyrrole methylase" FT /evidence="ECO:0000259|Pfam:PF00590" FT REGION 1..204 FT /note="Precorrin-2 dehydrogenase / sirohydrochlorin FT ferrochelatase" FT /evidence="ECO:0000256|HAMAP-Rule:MF_01646" FT REGION 216..457 FT /note="Uroporphyrinogen-III C-methyltransferase" FT /evidence="ECO:0000256|HAMAP-Rule:MF_01646" FT ACT_SITE 248 FT /note="Proton acceptor" FT /evidence="ECO:0000256|HAMAP-Rule:MF_01646, FT ECO:0000256|PIRSR:PIRSR036426-1" FT ACT_SITE 270 FT /note="Proton donor" FT /evidence="ECO:0000256|HAMAP-Rule:MF_01646, FT ECO:0000256|PIRSR:PIRSR036426-1" FT BINDING 22..23 FT /ligand="NAD(+)" FT /ligand_id="ChEBI:CHEBI:57540" FT /evidence="ECO:0000256|HAMAP-Rule:MF_01646" FT BINDING 43..44 FT /ligand="NAD(+)" FT /ligand_id="ChEBI:CHEBI:57540" FT /evidence="ECO:0000256|HAMAP-Rule:MF_01646" FT BINDING 225 FT /ligand="S-adenosyl-L-methionine" FT /ligand_id="ChEBI:CHEBI:59789" FT /evidence="ECO:0000256|HAMAP-Rule:MF_01646" FT BINDING 301..303 FT /ligand="S-adenosyl-L-methionine" FT /ligand_id="ChEBI:CHEBI:59789" FT /evidence="ECO:0000256|HAMAP-Rule:MF_01646" FT BINDING 306 FT /ligand="S-adenosyl-L-methionine" FT /ligand_id="ChEBI:CHEBI:59789" FT /evidence="ECO:0000256|HAMAP-Rule:MF_01646" FT BINDING 331..332 FT /ligand="S-adenosyl-L-methionine" FT /ligand_id="ChEBI:CHEBI:59789" FT /evidence="ECO:0000256|HAMAP-Rule:MF_01646" FT BINDING 382 FT /ligand="S-adenosyl-L-methionine" FT /ligand_id="ChEBI:CHEBI:59789" FT /evidence="ECO:0000256|HAMAP-Rule:MF_01646" FT BINDING 411 FT /ligand="S-adenosyl-L-methionine" FT /ligand_id="ChEBI:CHEBI:59789" FT /evidence="ECO:0000256|HAMAP-Rule:MF_01646" FT MOD_RES 128 FT /note="Phosphoserine" FT /evidence="ECO:0000256|HAMAP-Rule:MF_01646" SQ SEQUENCE 457 AA; 50128 MW; 5B2A57B48D62A3AF CRC64; MDHLPIFCQL RDRDCLIVGG GDVAERKARL LLEAGARLTV NALTFIPQFT VWANEGMLTL VEGPFDETLL DSCWLAIAAT DDDTVNQRVS EAAESRRIFC NVVDAPKAAS FIMPSIIDRS PLMVAVSSGG TSPVLARLLR EKLESLLPQH LGQVARYAGQ LRARVKKQFA TMGERRRFWE KFFVNDRLAQ SLANADEKAV NATTEHLFSE PLDHRGEVVL VGAGPGDAGL LTLKGLQQIQ QADIVVYDRL VSDDIMNLVR RDADRVFVGK RAGYHCVPQE EINQILLREA QKGKRVVRLK GGDPFIFGRG GEELETLCHA GIPFSVVPGI TAASGCSAYS GIPLTHRDYA QSVRLVTGHL KTGGELDWEN LAAEKQTLVF YMGLNQAATI QEKLIAFGMQ ADMPVALVEN GTSVKQRVVH GVLTQLGELA QQVESPALII VGRVVGLRDK LNWFSNH //