ID A0A3A3KD82_SALMO Unreviewed; 457 AA. AC A0A3A3KD82; DT 05-DEC-2018, integrated into UniProtKB/TrEMBL. DT 05-DEC-2018, sequence version 1. DT 22-APR-2020, entry version 13. DE RecName: Full=Siroheme synthase {ECO:0000256|HAMAP-Rule:MF_01646}; DE Includes: DE RecName: Full=Uroporphyrinogen-III C-methyltransferase {ECO:0000256|HAMAP-Rule:MF_01646}; DE Short=Urogen III methylase {ECO:0000256|HAMAP-Rule:MF_01646}; DE EC=2.1.1.107 {ECO:0000256|HAMAP-Rule:MF_01646}; DE AltName: Full=SUMT {ECO:0000256|HAMAP-Rule:MF_01646}; DE AltName: Full=Uroporphyrinogen III methylase {ECO:0000256|HAMAP-Rule:MF_01646}; DE Short=UROM {ECO:0000256|HAMAP-Rule:MF_01646}; DE Includes: DE RecName: Full=Precorrin-2 dehydrogenase {ECO:0000256|HAMAP-Rule:MF_01646}; DE EC=1.3.1.76 {ECO:0000256|HAMAP-Rule:MF_01646}; DE Includes: DE RecName: Full=Sirohydrochlorin ferrochelatase {ECO:0000256|HAMAP-Rule:MF_01646}; DE EC=4.99.1.4 {ECO:0000256|HAMAP-Rule:MF_01646}; GN Name=cobA {ECO:0000313|EMBL:EAA5755080.1}; GN Synonyms=cysG {ECO:0000256|HAMAP-Rule:MF_01646, GN ECO:0000313|EMBL:EDI2489406.1}; GN ORFNames=A4W27_12685 {ECO:0000313|EMBL:ECT6369364.1}, A7H78_14325 GN {ECO:0000313|EMBL:ECU6781968.1}, AAA83_00755 GN {ECO:0000313|EMBL:ECV8598069.1}, AAN00_09060 GN {ECO:0000313|EMBL:EAB5747702.1}, ABC80_03765 GN {ECO:0000313|EMBL:ECW5083772.1}, ABQ32_21105 GN {ECO:0000313|EMBL:ECV8680761.1}, AGE01_13850 GN {ECO:0000313|EMBL:ECS6048301.1}, AH152_07815 GN {ECO:0000313|EMBL:ECX6293266.1}, AHW55_06995 GN {ECO:0000313|EMBL:ECZ5382779.1}, AHY82_05955 GN {ECO:0000313|EMBL:ECV9755739.1}, AHZ57_11095 GN {ECO:0000313|EMBL:ECW6277666.1}, AIG37_12450 GN {ECO:0000313|EMBL:ECZ7803670.1}, AIG40_13175 GN {ECO:0000313|EMBL:ECZ7992705.1}, APO85_22565 GN {ECO:0000313|EMBL:ECX1556874.1}, ATO22_22235 GN {ECO:0000313|EMBL:ECX3292214.1}, AU259_12670 GN {ECO:0000313|EMBL:ECY3344894.1}, AUB14_16295 GN {ECO:0000313|EMBL:EBW5856307.1}, AVH73_20640 GN {ECO:0000313|EMBL:ECY9608729.1}, AXU14_19530 GN {ECO:0000313|EMBL:EDA0012506.1}, AXU21_23170 GN {ECO:0000313|EMBL:EAA9237225.1}, AXU32_12220 GN {ECO:0000313|EMBL:EDB4301317.1}, AXU38_13655 GN {ECO:0000313|EMBL:EAA5755080.1}, B0D32_12790 GN {ECO:0000313|EMBL:EDF3868613.1}, B4W87_11580 GN {ECO:0000313|EMBL:EDF8046213.1}, B5881_01010 GN {ECO:0000313|EMBL:EDG1349773.1}, B6438_13505 GN {ECO:0000313|EMBL:EDF8424727.1}, B6C67_06015 GN {ECO:0000313|EMBL:EDG0896523.1}, B9F35_11955 GN {ECO:0000313|EMBL:EDG6967856.1}, BGP51_12190 GN {ECO:0000313|EMBL:ECT7834235.1}, BIW02_13375 GN {ECO:0000313|EMBL:EDC6720060.1}, BS131_20695 GN {ECO:0000313|EMBL:EDI2489406.1}, BW881_12135 GN {ECO:0000313|EMBL:EBW9014067.1}, BZ049_13945 GN {ECO:0000313|EMBL:EDF3746908.1}, C2689_15270 GN {ECO:0000313|EMBL:ECY6787821.1}, C5M29_11430 GN {ECO:0000313|EMBL:ECU0262994.1}, CDF98_09540 GN {ECO:0000313|EMBL:EDI2972011.1}, CDG04_09550 GN {ECO:0000313|EMBL:EDI2922238.1}, CDK56_12575 GN {ECO:0000313|EMBL:EDI2830819.1}, CDK60_10680 GN {ECO:0000313|EMBL:EDI2909165.1}, CEA34_13350 GN {ECO:0000313|EMBL:EDI3638726.1}, CJK98_11975 GN {ECO:0000313|EMBL:ECS7617439.1}, CM22_11685 GN {ECO:0000313|EMBL:EAA7519904.1}, CVB19_01830 GN {ECO:0000313|EMBL:EAA8532710.1}, CW117_11520 GN {ECO:0000313|EMBL:ECS8999704.1}, D0180_12775 GN {ECO:0000313|EMBL:ECT3028538.1}, D0215_03540 GN {ECO:0000313|EMBL:ECV0718804.1}, D2K48_02850 GN {ECO:0000313|EMBL:ECU4075207.1}, D2K49_02285 GN {ECO:0000313|EMBL:ECT3325251.1}, D3L82_11070 GN {ECO:0000313|EMBL:ECV0570431.1}, D3L96_21165 GN {ECO:0000313|EMBL:ECT3577477.1}, D6317_03655 GN {ECO:0000313|EMBL:EBZ0625307.1}, D6R56_04475 GN {ECO:0000313|EMBL:EBY8466085.1}, D7O42_10915 GN {ECO:0000313|EMBL:EBZ1034862.1}, D9U82_00785 GN {ECO:0000313|EMBL:EBZ3293249.1}, D9Z08_02570 GN {ECO:0000313|EMBL:EBZ3336464.1}, DK664_00780 GN {ECO:0000313|EMBL:ECS9166961.1}, DN091_09070 GN {ECO:0000313|EMBL:EBV2229751.1}, DN315_11515 GN {ECO:0000313|EMBL:EBV2633473.1}, DO829_05745 GN {ECO:0000313|EMBL:ECV3374124.1}, DO851_03780 GN {ECO:0000313|EMBL:ECU1648370.1}, DOA62_18105 GN {ECO:0000313|EMBL:EAA3557976.1}, DP697_23525 GN {ECO:0000313|EMBL:EBW6729891.1}, DPD34_07780 GN {ECO:0000313|EMBL:EBW3249377.1}, DPE74_06105 GN {ECO:0000313|EMBL:EBW3454518.1}, DPL19_03830 GN {ECO:0000313|EMBL:ECV3149336.1}, DQQ77_10500 GN {ECO:0000313|EMBL:ECV2817836.1}, DQS63_22775 GN {ECO:0000313|EMBL:EBS4622864.1}, DR817_06875 GN {ECO:0000313|EMBL:ECT0761347.1}, DT992_02860 GN {ECO:0000313|EMBL:ECV2332036.1}, DU033_02045 GN {ECO:0000313|EMBL:ECT1447978.1}, DUR40_08190 GN {ECO:0000313|EMBL:EBY0617106.1}, DUZ79_19460 GN {ECO:0000313|EMBL:ECV5493333.1}, DVF18_19885 GN {ECO:0000313|EMBL:EBY2678738.1}, DWS41_21465 GN {ECO:0000313|EMBL:ECU3035994.1}, DWU36_01950 GN {ECO:0000313|EMBL:ECU2886735.1}, DYN58_13705 GN {ECO:0000313|EMBL:ECV1369222.1}, DYO72_17690 GN {ECO:0000313|EMBL:ECU3335379.1}, DYT96_08350 GN {ECO:0000313|EMBL:ECU3685001.1}, DZG42_02665 GN {ECO:0000313|EMBL:ECV1014629.1}, E0U36_04570 GN {ECO:0000313|EMBL:ECB6706091.1}, E1406_10255 GN {ECO:0000313|EMBL:ECG5541193.1}, E3B49_03600 GN {ECO:0000313|EMBL:EBO2003073.1}, E4933_11310 GN {ECO:0000313|EMBL:KAA7153371.1}, E4S88_10635 GN {ECO:0000313|EMBL:EBO8724379.1}, E5453_08715 GN {ECO:0000313|EMBL:EBM8713119.1}, ECC84_11055 GN {ECO:0000313|EMBL:EBZ5448309.1}, EER82_11860 GN {ECO:0000313|EMBL:EBZ6770628.1}, EIC46_01300 GN {ECO:0000313|EMBL:EBO8585431.1}, EP765_09060 GN {ECO:0000313|EMBL:ECA7972613.1}, ERF79_03020 GN {ECO:0000313|EMBL:ECA8572825.1}, FEH78_05450 GN {ECO:0000313|EMBL:EBM9175818.1}, FJJ53_08865 GN {ECO:0000313|EMBL:EBG8086904.1}, GBH27_09500 GN {ECO:0000313|EMBL:EDF9740439.1}, II32_05055 GN {ECO:0000313|EMBL:ECW6425007.1}, QB52_09305 GN {ECO:0000313|EMBL:EAA7131451.1}, RU33_11720 GN {ECO:0000313|EMBL:ECU5582395.1}, UK00_22635 GN {ECO:0000313|EMBL:EAA8125631.1}, XU87_13655 GN {ECO:0000313|EMBL:EAA9554405.1}; OS Salmonella montevideo. OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; OC Enterobacteriaceae; Salmonella. OX NCBI_TaxID=115981 {ECO:0000313|EMBL:EAA5755080.1}; RN [1] {ECO:0000313|EMBL:EAA5755080.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=ADRDL-1061 {ECO:0000313|EMBL:EDF8424727.1}, ADRDL-1578 RC {ECO:0000313|EMBL:EAA8532710.1}, CFSAN004243 RC {ECO:0000313|EMBL:EAA8125631.1}, CFSAN012296 RC {ECO:0000313|EMBL:ECS6048301.1}, CFSAN025698 RC {ECO:0000313|EMBL:ECU5582395.1}, CFSAN032143 RC {ECO:0000313|EMBL:EDA0012506.1}, CFSAN032150 RC {ECO:0000313|EMBL:EAA9237225.1}, CFSAN032161 RC {ECO:0000313|EMBL:EDB4301317.1}, CFSAN032167 RC {ECO:0000313|EMBL:EAA5755080.1}, CVM-N26923 RC {ECO:0000313|EMBL:EBW5856307.1}, FDA00001001 RC {ECO:0000313|EMBL:ECZ5382779.1}, FDA00001465 RC {ECO:0000313|EMBL:ECV9755739.1}, FDA00001553 RC {ECO:0000313|EMBL:ECW6277666.1}, FDA00001773 RC {ECO:0000313|EMBL:ECX6293266.1}, FDA00004064 RC {ECO:0000313|EMBL:ECZ7803670.1}, FDA00004067 RC {ECO:0000313|EMBL:ECZ7992705.1}, FSIS11809966 RC {ECO:0000313|EMBL:ECS9166961.1}, FSIS11810956 RC {ECO:0000313|EMBL:ECV2817836.1}, FSIS11811659 RC {ECO:0000313|EMBL:ECV2332036.1}, FSIS11811732 RC {ECO:0000313|EMBL:ECT1447978.1}, FSIS11812028 RC {ECO:0000313|EMBL:ECU3035994.1}, FSIS11812188 RC {ECO:0000313|EMBL:ECU2886735.1}, FSIS11812555 RC {ECO:0000313|EMBL:ECU3685001.1}, FSIS11813009 RC {ECO:0000313|EMBL:ECV1014629.1}, FSIS11813078 RC {ECO:0000313|EMBL:ECV0718804.1}, FSIS11813231 RC {ECO:0000313|EMBL:ECV0570431.1}, FSIS11813436 RC {ECO:0000313|EMBL:ECT3577477.1}, FSIS11813543 RC {ECO:0000313|EMBL:ECT3325251.1}, FSIS11813547 RC {ECO:0000313|EMBL:ECU4075207.1}, FSIS11814491 RC {ECO:0000313|EMBL:EBZ3336464.1}, FSIS11919213 RC {ECO:0000313|EMBL:EBO8724379.1}, FSIS1503492 RC {ECO:0000313|EMBL:ECX3292214.1}, FSIS1606211 RC {ECO:0000313|EMBL:ECT6369364.1}, FSIS1701791 RC {ECO:0000313|EMBL:EDI3638726.1}, FSIS1709880 RC {ECO:0000313|EMBL:EDF3868613.1}, FSIS1710584 RC {ECO:0000313|EMBL:EDF8046213.1}, FSIS31800893 RC {ECO:0000313|EMBL:ECT3028538.1}, FSIS31801083 RC {ECO:0000313|EMBL:EBZ0625307.1}, HIY0203 RC {ECO:0000313|EMBL:EBW9014067.1}, MDH-2014-00193 RC {ECO:0000313|EMBL:EAA7519904.1}, MDH-2014-00368 RC {ECO:0000313|EMBL:ECW6425007.1, ECO:0000313|Proteomes:UP000419897}, RC NY-N13154 {ECO:0000313|EMBL:EAA9554405.1}, and TX-ACF1401827 RC {ECO:0000313|EMBL:ECW5083772.1}; RG GenomeTrakr network: Whole genome sequencing for foodborne pathogen traceback; RL Submitted (JUN-2018) to the EMBL/GenBank/DDBJ databases. RN [2] {ECO:0000313|EMBL:ECS8999704.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=CVM N17S1479 {ECO:0000313|EMBL:ECU3335379.1}, CVM N17S499 RC {ECO:0000313|EMBL:ECV1369222.1}, FSIS11705537 RC {ECO:0000313|EMBL:ECS8999704.1}, FSIS11807106 RC {ECO:0000313|EMBL:ECY6787821.1}, FSIS11807534 RC {ECO:0000313|EMBL:ECU0262994.1}, FSIS11810214 RC {ECO:0000313|EMBL:ECV3374124.1}, FSIS11810439 RC {ECO:0000313|EMBL:ECU1648370.1}, FSIS11810643 RC {ECO:0000313|EMBL:ECV3149336.1}, FSIS11811098 RC {ECO:0000313|EMBL:ECT0761347.1}, FSIS11811961 RC {ECO:0000313|EMBL:ECV5493333.1}, FSIS11814638 RC {ECO:0000313|EMBL:EBZ3293249.1}, FSIS11815944 RC {ECO:0000313|EMBL:EBO8585431.1}, FSIS11917052 RC {ECO:0000313|EMBL:ECA7972613.1}, FSIS11918954 RC {ECO:0000313|EMBL:EBO2003073.1}, FSIS11919668 RC {ECO:0000313|EMBL:EBM8713119.1}, FSIS11920988 RC {ECO:0000313|EMBL:EBM9175818.1}, FSIS1500714 RC {ECO:0000313|EMBL:ECX1556874.1}, FSIS1504186 RC {ECO:0000313|EMBL:ECY3344894.1}, FSIS1606411 RC {ECO:0000313|EMBL:ECU6781968.1}, FSIS1607454 RC {ECO:0000313|EMBL:ECT7834235.1}, FSIS1607655 RC {ECO:0000313|EMBL:EDC6720060.1}, FSIS1700054 RC {ECO:0000313|EMBL:EDG0896523.1}, FSIS1701071 RC {ECO:0000313|EMBL:EDI2909165.1}, and FSIS1701094 RC {ECO:0000313|EMBL:EDI2830819.1}; RG NARMS: The National Antimicrobial Resistance Monitoring System; RL Submitted (JUL-2018) to the EMBL/GenBank/DDBJ databases. RN [3] {ECO:0000313|EMBL:ECS7617439.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=PNUSAS005717 {ECO:0000313|EMBL:EDI2489406.1}, PNUSAS007847 RC {ECO:0000313|EMBL:EDF3746908.1}, PNUSAS010114 RC {ECO:0000313|EMBL:EDG1349773.1}, PNUSAS012048 RC {ECO:0000313|EMBL:EDG6967856.1}, PNUSAS013952 RC {ECO:0000313|EMBL:EDI2972011.1}, PNUSAS013962 RC {ECO:0000313|EMBL:EDI2922238.1}, and PNUSAS019757 RC {ECO:0000313|EMBL:ECS7617439.1}; RG PulseNet: The National Subtyping Network for Foodborne Disease Surveillance; RA Tarr C.L., Trees E., Katz L.S., Carleton-Romer H.A., Stroika S., RA Kucerova Z., Roache K.F., Sabol A.L., Besser J., Gerner-Smidt P.; RL Submitted (JUL-2018) to the EMBL/GenBank/DDBJ databases. RN [4] {ECO:0000313|EMBL:EAA7131451.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=133983 {ECO:0000313|EMBL:EBW6729891.1}, 13831 RC {ECO:0000313|EMBL:ECV8680761.1}, 14493 RC {ECO:0000313|EMBL:ECV8598069.1}, 155638 RC {ECO:0000313|EMBL:EBY2678738.1}, 160770 RC {ECO:0000313|EMBL:EBS4622864.1}, 197101 RC {ECO:0000313|EMBL:ECG5541193.1}, 260057 RC {ECO:0000313|EMBL:EBY0617106.1}, 280672 RC {ECO:0000313|EMBL:EBV2633473.1}, 319650 RC {ECO:0000313|EMBL:ECB6706091.1}, 387053 RC {ECO:0000313|EMBL:EBW3249377.1}, 401701 RC {ECO:0000313|EMBL:EBW3454518.1}, 473905 RC {ECO:0000313|EMBL:EAA3557976.1}, 490270 RC {ECO:0000313|EMBL:EBV2229751.1}, 56964 RC {ECO:0000313|EMBL:ECY9608729.1}, 575102 RC {ECO:0000313|EMBL:EBZ1034862.1}, 607266 RC {ECO:0000313|EMBL:EBY8466085.1}, 621734 RC {ECO:0000313|EMBL:EBZ5448309.1}, 624443 RC {ECO:0000313|EMBL:EBZ6770628.1}, 64947 RC {ECO:0000313|EMBL:EAB5747702.1}, 664953 RC {ECO:0000313|EMBL:ECA8572825.1}, 758106 RC {ECO:0000313|EMBL:EBG8086904.1}, 818883 RC {ECO:0000313|EMBL:EDF9740439.1}, and H120360356 RC {ECO:0000313|EMBL:EAA7131451.1}; RA Ashton P.M., Dallman T., Nair S., De Pinna E., Peters T., Grant K.; RL Submitted (JUL-2018) to the EMBL/GenBank/DDBJ databases. RN [5] {ECO:0000313|EMBL:KAA7153371.1, ECO:0000313|Proteomes:UP000322618} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=ME2L-19-263 {ECO:0000313|EMBL:KAA7153371.1, RC ECO:0000313|Proteomes:UP000322618}; RX PubMed=31527278; DOI=.1073/pnas.1907787116; RA Rudman S.M., Greenblum S., Hughes R.C., Rajpurohit S., Kiratli O., RA Lowder D.B., Lemmon S.G., Petrov D.A., Chaston J.M., Schmidt P.; RT "Microbiome composition shapes rapid genomic adaptation of Drosophila RT melanogaster."; RL Proc. Natl. Acad. Sci. U.S.A. 116:20025-20032(2019). RN [6] {ECO:0000313|EMBL:KAA7153371.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=ME2L-19-263 {ECO:0000313|EMBL:KAA7153371.1}; RA Levent G., Schlochtermeier A., Ives S.E., Norman K.N., Lawhon S.D., RA Loneragan G.H., Anderson R.C., Scott H.M.; RL Submitted (MAR-2019) to the EMBL/GenBank/DDBJ databases. CC -!- FUNCTION: Multifunctional enzyme that catalyzes the SAM-dependent CC methylations of uroporphyrinogen III at position C-2 and C-7 to form CC precorrin-2 via precorrin-1. Then it catalyzes the NAD-dependent ring CC dehydrogenation of precorrin-2 to yield sirohydrochlorin. Finally, it CC catalyzes the ferrochelation of sirohydrochlorin to yield siroheme. CC {ECO:0000256|HAMAP-Rule:MF_01646, ECO:0000256|SAAS:SAAS00971394}. CC -!- CATALYTIC ACTIVITY: CC Reaction=2 H(+) + siroheme = Fe(2+) + sirohydrochlorin; CC Xref=Rhea:RHEA:24360, ChEBI:CHEBI:15378, ChEBI:CHEBI:29033, CC ChEBI:CHEBI:58351, ChEBI:CHEBI:60052; EC=4.99.1.4; CC Evidence={ECO:0000256|HAMAP-Rule:MF_01646, CC ECO:0000256|SAAS:SAAS01123671}; CC -!- CATALYTIC ACTIVITY: CC Reaction=2 S-adenosyl-L-methionine + uroporphyrinogen III = H(+) + CC precorrin-2 + 2 S-adenosyl-L-homocysteine; Xref=Rhea:RHEA:32459, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57308, ChEBI:CHEBI:57856, CC ChEBI:CHEBI:58827, ChEBI:CHEBI:59789; EC=2.1.1.107; CC Evidence={ECO:0000256|HAMAP-Rule:MF_01646, CC ECO:0000256|SAAS:SAAS01116480}; CC -!- CATALYTIC ACTIVITY: CC Reaction=NAD(+) + precorrin-2 = 2 H(+) + NADH + sirohydrochlorin; CC Xref=Rhea:RHEA:15613, ChEBI:CHEBI:15378, ChEBI:CHEBI:57540, CC ChEBI:CHEBI:57945, ChEBI:CHEBI:58351, ChEBI:CHEBI:58827; EC=1.3.1.76; CC Evidence={ECO:0000256|HAMAP-Rule:MF_01646, CC ECO:0000256|SAAS:SAAS01123666}; CC -!- PATHWAY: Cofactor biosynthesis; adenosylcobalamin biosynthesis; CC sirohydrochlorin from precorrin-2: step 1/1. {ECO:0000256|HAMAP- CC Rule:MF_01646, ECO:0000256|SAAS:SAAS01024981}. CC -!- PATHWAY: Porphyrin-containing compound metabolism; siroheme CC biosynthesis; precorrin-2 from uroporphyrinogen III: step 1/1. CC {ECO:0000256|HAMAP-Rule:MF_01646, ECO:0000256|SAAS:SAAS00971402}. CC -!- PATHWAY: Porphyrin-containing compound metabolism; siroheme CC biosynthesis; siroheme from sirohydrochlorin: step 1/1. CC {ECO:0000256|HAMAP-Rule:MF_01646, ECO:0000256|SAAS:SAAS01024978}. CC -!- PATHWAY: Porphyrin-containing compound metabolism; siroheme CC biosynthesis; sirohydrochlorin from precorrin-2: step 1/1. CC {ECO:0000256|HAMAP-Rule:MF_01646, ECO:0000256|SAAS:SAAS01024982}. CC -!- SIMILARITY: Belongs to the precorrin methyltransferase family. CC {ECO:0000256|RuleBase:RU003960}. CC -!- SIMILARITY: In the C-terminal section; belongs to the precorrin CC methyltransferase family. {ECO:0000256|HAMAP-Rule:MF_01646, CC ECO:0000256|SAAS:SAAS00971398}. CC -!- SIMILARITY: In the N-terminal section; belongs to the precorrin-2 CC dehydrogenase / sirohydrochlorin ferrochelatase family. CC {ECO:0000256|HAMAP-Rule:MF_01646, ECO:0000256|SAAS:SAAS00971404}. CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ CC whole genome shotgun (WGS) entry which is preliminary data. CC {ECO:0000313|EMBL:EAA5755080.1}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AAABFP010000062; EAA3557976.1; -; Genomic_DNA. DR EMBL; AAABXU010000007; EAA5755080.1; -; Genomic_DNA. DR EMBL; AAACJZ010000006; EAA7131451.1; -; Genomic_DNA. DR EMBL; AAACMB010000004; EAA7519904.1; -; Genomic_DNA. DR EMBL; AAACQX010000028; EAA8125631.1; -; Genomic_DNA. DR EMBL; AAACTW010000002; EAA8532710.1; -; Genomic_DNA. DR EMBL; AAACZB010000023; EAA9237225.1; -; Genomic_DNA. DR EMBL; AAADCM010000006; EAA9554405.1; -; Genomic_DNA. DR EMBL; AAAFDE010000006; EAB5747702.1; -; Genomic_DNA. DR EMBL; AAFKBF010000006; EBG8086904.1; -; Genomic_DNA. DR EMBL; AAGDZM010000006; EBM8713119.1; -; Genomic_DNA. DR EMBL; AAGEDJ010000003; EBM9175818.1; -; Genomic_DNA. DR EMBL; AAGHTI010000003; EBO2003073.1; -; Genomic_DNA. DR EMBL; AAGJVW010000001; EBO8585431.1; -; Genomic_DNA. DR EMBL; AAGJXF010000008; EBO8724379.1; -; Genomic_DNA. DR EMBL; AAGVOP010000264; EBS4622864.1; -; Genomic_DNA. DR EMBL; AAHENV010000007; EBV2229751.1; -; Genomic_DNA. DR EMBL; AAHERQ010000008; EBV2633473.1; -; Genomic_DNA. DR EMBL; AAHIAJ010000006; EBW3249377.1; -; Genomic_DNA. DR EMBL; AAHIBZ010000005; EBW3454518.1; -; Genomic_DNA. DR EMBL; AAHHIL010000056; EBW5856307.1; -; Genomic_DNA. DR EMBL; AAHIXI010000040; EBW6729891.1; -; Genomic_DNA. DR EMBL; AAHJPH010000007; EBW9014067.1; -; Genomic_DNA. DR EMBL; AAHNAA010000006; EBY0617106.1; -; Genomic_DNA. DR EMBL; AAHNQG010000054; EBY2678738.1; -; Genomic_DNA. DR EMBL; AAHPGB010000004; EBY8466085.1; -; Genomic_DNA. DR EMBL; AAHPXJ010000003; EBZ0625307.1; -; Genomic_DNA. DR EMBL; AAHQBH010000007; EBZ1034862.1; -; Genomic_DNA. DR EMBL; AAHQUA010000001; EBZ3293249.1; -; Genomic_DNA. DR EMBL; AAHQUI010000002; EBZ3336464.1; -; Genomic_DNA. DR EMBL; AAHRME010000008; EBZ5448309.1; -; Genomic_DNA. DR EMBL; AAHRXI010000007; EBZ6770628.1; -; Genomic_DNA. DR EMBL; AAHVMW010000004; ECA7972613.1; -; Genomic_DNA. DR EMBL; AAHVSA010000002; ECA8572825.1; -; Genomic_DNA. DR EMBL; AAHYIA010000002; ECB6706091.1; -; Genomic_DNA. DR EMBL; AAIOTV010000008; ECG5541193.1; -; Genomic_DNA. DR EMBL; AAKKCM010000008; ECS6048301.1; -; Genomic_DNA. DR EMBL; AAKKPQ010000004; ECS7617439.1; -; Genomic_DNA. DR EMBL; AAKKUG010000004; ECS8999704.1; -; Genomic_DNA. DR EMBL; AAKLCW010000001; ECS9166961.1; -; Genomic_DNA. DR EMBL; AAKLPX010000004; ECT0761347.1; -; Genomic_DNA. DR EMBL; AAKLVM010000002; ECT1447978.1; -; Genomic_DNA. DR EMBL; AAKMIM010000009; ECT3028538.1; -; Genomic_DNA. DR EMBL; AAKMKW010000002; ECT3325251.1; -; Genomic_DNA. DR EMBL; AAKMMZ010000050; ECT3577477.1; -; Genomic_DNA. DR EMBL; AAKNKC010000004; ECT6369364.1; -; Genomic_DNA. DR EMBL; AAKNWF010000005; ECT7834235.1; -; Genomic_DNA. DR EMBL; AAKOQL010000006; ECU0262994.1; -; Genomic_DNA. DR EMBL; AAKPBW010000003; ECU1648370.1; -; Genomic_DNA. DR EMBL; AAKPMA010000003; ECU2886735.1; -; Genomic_DNA. DR EMBL; AAKPND010000052; ECU3035994.1; -; Genomic_DNA. DR EMBL; AAKPPR010000007; ECU3335379.1; -; Genomic_DNA. DR EMBL; AAKPRQ010000007; ECU3685001.1; -; Genomic_DNA. DR EMBL; AAKPVL010000002; ECU4075207.1; -; Genomic_DNA. DR EMBL; AAKQIB010000005; ECU5582395.1; -; Genomic_DNA. DR EMBL; AAKQRS010000006; ECU6781968.1; -; Genomic_DNA. DR EMBL; AAKSVN010000008; ECV0570431.1; -; Genomic_DNA. DR EMBL; AAKSUE010000003; ECV0718804.1; -; Genomic_DNA. DR EMBL; AAKSRY010000002; ECV1014629.1; -; Genomic_DNA. DR EMBL; AAKSQM010000007; ECV1369222.1; -; Genomic_DNA. DR EMBL; AAKSJB010000002; ECV2332036.1; -; Genomic_DNA. DR EMBL; AAKSDW010000006; ECV2817836.1; -; Genomic_DNA. DR EMBL; AAKSCW010000003; ECV3149336.1; -; Genomic_DNA. DR EMBL; AAKSCO010000004; ECV3374124.1; -; Genomic_DNA. DR EMBL; AAKSKE010000059; ECV5493333.1; -; Genomic_DNA. DR EMBL; AAKUNK010000001; ECV8598069.1; -; Genomic_DNA. DR EMBL; AAKUOB010000035; ECV8680761.1; -; Genomic_DNA. DR EMBL; AAKUXP010000005; ECV9755739.1; -; Genomic_DNA. DR EMBL; AAKWSS010000003; ECW5083772.1; -; Genomic_DNA. DR EMBL; AAKXFW010000003; ECW6277666.1; -; Genomic_DNA. DR EMBL; AAKXFP010000003; ECW6425007.1; -; Genomic_DNA. DR EMBL; AAKYVF010000039; ECX1556874.1; -; Genomic_DNA. DR EMBL; AAKZKE010000038; ECX3292214.1; -; Genomic_DNA. DR EMBL; AAKZQR010000004; ECX6293266.1; -; Genomic_DNA. DR EMBL; AALCUX010000007; ECY3344894.1; -; Genomic_DNA. DR EMBL; AALEDO010000008; ECY6787821.1; -; Genomic_DNA. DR EMBL; AALGFK010000042; ECY9608729.1; -; Genomic_DNA. DR EMBL; AALGYC010000003; ECZ5382779.1; -; Genomic_DNA. DR EMBL; AALHUF010000006; ECZ7803670.1; -; Genomic_DNA. DR EMBL; AALHUL010000007; ECZ7992705.1; -; Genomic_DNA. DR EMBL; AALIOX010000018; EDA0012506.1; -; Genomic_DNA. DR EMBL; AALNLT010000029; EDB4301317.1; -; Genomic_DNA. DR EMBL; AALRRW010000008; EDC6720060.1; -; Genomic_DNA. DR EMBL; AAMAPH010000009; EDF3746908.1; -; Genomic_DNA. DR EMBL; AAMARA010000006; EDF3868613.1; -; Genomic_DNA. DR EMBL; AAMBYF010000006; EDF8046213.1; -; Genomic_DNA. DR EMBL; AAMCCS010000007; EDF8424727.1; -; Genomic_DNA. DR EMBL; AAMCON010000007; EDF9740439.1; -; Genomic_DNA. DR EMBL; AAMCZA010000004; EDG0896523.1; -; Genomic_DNA. DR EMBL; AAMDCD010000001; EDG1349773.1; -; Genomic_DNA. DR EMBL; AAMFAQ010000005; EDG6967856.1; -; Genomic_DNA. DR EMBL; AAMKGK010000033; EDI2489406.1; -; Genomic_DNA. DR EMBL; AAMKKW010000007; EDI2830819.1; -; Genomic_DNA. DR EMBL; AAMKLQ010000006; EDI2909165.1; -; Genomic_DNA. DR EMBL; AAMKMP010000005; EDI2922238.1; -; Genomic_DNA. DR EMBL; AAMKMT010000006; EDI2972011.1; -; Genomic_DNA. DR EMBL; AAMKRW010000008; EDI3638726.1; -; Genomic_DNA. DR EMBL; SRBT01000004; KAA7153371.1; -; Genomic_DNA. DR RefSeq; WP_000349904.1; NZ_VCUN02000005.1. DR UniPathway; UPA00148; UER00222. DR UniPathway; UPA00262; UER00211. DR Proteomes; UP000322618; Unassembled WGS sequence. DR Proteomes; UP000419897; Unassembled WGS sequence. DR GO; GO:0051287; F:NAD binding; IEA:InterPro. DR GO; GO:0043115; F:precorrin-2 dehydrogenase activity; IEA:UniProtKB-UniRule. DR GO; GO:0051266; F:sirohydrochlorin ferrochelatase activity; IEA:UniProtKB-EC. DR GO; GO:0004851; F:uroporphyrin-III C-methyltransferase activity; IEA:UniProtKB-UniRule. DR GO; GO:0009236; P:cobalamin biosynthetic process; IEA:UniProtKB-UniRule. DR GO; GO:0032259; P:methylation; IEA:UniProtKB-KW. DR GO; GO:0019354; P:siroheme biosynthetic process; IEA:UniProtKB-UniRule. DR CDD; cd11642; SUMT; 1. DR Gene3D; 1.10.8.210; -; 1. DR Gene3D; 3.30.950.10; -; 1. DR Gene3D; 3.40.1010.10; -; 1. DR HAMAP; MF_01646; Siroheme_synth; 1. DR InterPro; IPR000878; 4pyrrol_Mease. DR InterPro; IPR035996; 4pyrrol_Methylase_sf. DR InterPro; IPR014777; 4pyrrole_Mease_sub1. DR InterPro; IPR014776; 4pyrrole_Mease_sub2. DR InterPro; IPR006366; CobA/CysG_C. DR InterPro; IPR036291; NAD(P)-bd_dom_sf. DR InterPro; IPR037115; Sirohaem_synt_dimer_dom_sf. DR InterPro; IPR012409; Sirohaem_synth. DR InterPro; IPR028281; Sirohaem_synthase_central. DR InterPro; IPR019478; Sirohaem_synthase_dimer_dom. DR InterPro; IPR006367; Sirohaem_synthase_N. DR InterPro; IPR003043; Uropor_MeTrfase_CS. DR Pfam; PF10414; CysG_dimeriser; 1. DR Pfam; PF14824; Sirohm_synth_M; 1. DR Pfam; PF00590; TP_methylase; 1. DR PIRSF; PIRSF036426; Sirohaem_synth; 1. DR SUPFAM; SSF51735; SSF51735; 1. DR SUPFAM; SSF53790; SSF53790; 1. DR TIGRFAMs; TIGR01469; cobA_cysG_Cterm; 1. DR TIGRFAMs; TIGR01470; cysG_Nterm; 1. DR PROSITE; PS00839; SUMT_1; 1. DR PROSITE; PS00840; SUMT_2; 1. PE 3: Inferred from homology; KW Cobalamin biosynthesis {ECO:0000256|HAMAP-Rule:MF_01646, KW ECO:0000256|SAAS:SAAS01024974}; Coiled coil {ECO:0000256|SAM:Coils}; KW Lyase {ECO:0000256|HAMAP-Rule:MF_01646, ECO:0000256|SAAS:SAAS01024979, KW ECO:0000313|EMBL:EDI2489406.1}; KW Methyltransferase {ECO:0000256|HAMAP-Rule:MF_01646, KW ECO:0000256|RuleBase:RU003960, ECO:0000256|SAAS:SAAS00467449, KW ECO:0000313|EMBL:EAA5755080.1}; KW Multifunctional enzyme {ECO:0000256|HAMAP-Rule:MF_01646, KW ECO:0000256|SAAS:SAAS01024984}; KW NAD {ECO:0000256|HAMAP-Rule:MF_01646, ECO:0000256|SAAS:SAAS01024985}; KW Oxidoreductase {ECO:0000256|HAMAP-Rule:MF_01646, KW ECO:0000256|SAAS:SAAS01024988, ECO:0000313|EMBL:EDI2489406.1}; KW Phosphoprotein {ECO:0000256|HAMAP-Rule:MF_01646}; KW Porphyrin biosynthesis {ECO:0000256|HAMAP-Rule:MF_01646, KW ECO:0000256|SAAS:SAAS01024977}; KW S-adenosyl-L-methionine {ECO:0000256|HAMAP-Rule:MF_01646, KW ECO:0000256|SAAS:SAAS00467481}; KW Transferase {ECO:0000256|HAMAP-Rule:MF_01646, KW ECO:0000256|RuleBase:RU003960, ECO:0000256|SAAS:SAAS00467429, KW ECO:0000313|EMBL:EAA5755080.1}. FT DOMAIN 119..145 FT /note="Sirohm_synth_M" FT /evidence="ECO:0000259|Pfam:PF14824" FT DOMAIN 150..207 FT /note="CysG_dimeriser" FT /evidence="ECO:0000259|Pfam:PF10414" FT DOMAIN 218..426 FT /note="TP_methylase" FT /evidence="ECO:0000259|Pfam:PF00590" FT NP_BIND 22..23 FT /note="NAD" FT /evidence="ECO:0000256|HAMAP-Rule:MF_01646" FT NP_BIND 43..44 FT /note="NAD" FT /evidence="ECO:0000256|HAMAP-Rule:MF_01646" FT REGION 1..204 FT /note="Precorrin-2 dehydrogenase / sirohydrochlorin FT ferrochelatase" FT /evidence="ECO:0000256|HAMAP-Rule:MF_01646" FT REGION 216..457 FT /note="Uroporphyrinogen-III C-methyltransferase" FT /evidence="ECO:0000256|HAMAP-Rule:MF_01646" FT REGION 301..303 FT /note="S-adenosyl-L-methionine binding" FT /evidence="ECO:0000256|HAMAP-Rule:MF_01646" FT REGION 331..332 FT /note="S-adenosyl-L-methionine binding" FT /evidence="ECO:0000256|HAMAP-Rule:MF_01646" FT COILED 185..205 FT /evidence="ECO:0000256|SAM:Coils" FT ACT_SITE 248 FT /note="Proton acceptor" FT /evidence="ECO:0000256|HAMAP-Rule:MF_01646, FT ECO:0000256|PIRSR:PIRSR036426-1" FT ACT_SITE 270 FT /note="Proton donor" FT /evidence="ECO:0000256|HAMAP-Rule:MF_01646, FT ECO:0000256|PIRSR:PIRSR036426-1" FT BINDING 225 FT /note="S-adenosyl-L-methionine; via carbonyl oxygen" FT /evidence="ECO:0000256|HAMAP-Rule:MF_01646" FT BINDING 306 FT /note="S-adenosyl-L-methionine; via carbonyl oxygen" FT /evidence="ECO:0000256|HAMAP-Rule:MF_01646" FT BINDING 382 FT /note="S-adenosyl-L-methionine; via amide nitrogen" FT /evidence="ECO:0000256|HAMAP-Rule:MF_01646" FT BINDING 411 FT /note="S-adenosyl-L-methionine; via amide nitrogen and FT carbonyl oxygen" FT /evidence="ECO:0000256|HAMAP-Rule:MF_01646" FT MOD_RES 128 FT /note="Phosphoserine" FT /evidence="ECO:0000256|HAMAP-Rule:MF_01646" SQ SEQUENCE 457 AA; 50128 MW; 5B2A57B48D62A3AF CRC64; MDHLPIFCQL RDRDCLIVGG GDVAERKARL LLEAGARLTV NALTFIPQFT VWANEGMLTL VEGPFDETLL DSCWLAIAAT DDDTVNQRVS EAAESRRIFC NVVDAPKAAS FIMPSIIDRS PLMVAVSSGG TSPVLARLLR EKLESLLPQH LGQVARYAGQ LRARVKKQFA TMGERRRFWE KFFVNDRLAQ SLANADEKAV NATTEHLFSE PLDHRGEVVL VGAGPGDAGL LTLKGLQQIQ QADIVVYDRL VSDDIMNLVR RDADRVFVGK RAGYHCVPQE EINQILLREA QKGKRVVRLK GGDPFIFGRG GEELETLCHA GIPFSVVPGI TAASGCSAYS GIPLTHRDYA QSVRLVTGHL KTGGELDWEN LAAEKQTLVF YMGLNQAATI QEKLIAFGMQ ADMPVALVEN GTSVKQRVVH GVLTQLGELA QQVESPALII VGRVVGLRDK LNWFSNH //