ID A0A352P2U5_9FIRM Unreviewed; 404 AA. AC A0A352P2U5; DT 07-NOV-2018, integrated into UniProtKB/TrEMBL. DT 07-NOV-2018, sequence version 1. DT 13-FEB-2019, entry version 4. DE RecName: Full=Homoserine dehydrogenase {ECO:0000256|RuleBase:RU000579}; DE EC=1.1.1.3 {ECO:0000256|RuleBase:RU000579}; GN ORFNames=DC053_07820 {ECO:0000313|EMBL:HBC99278.1}; OS Lachnoclostridium sp. OC Bacteria; Firmicutes; Clostridia; Clostridiales; Lachnospiraceae. OX NCBI_TaxID=2028282 {ECO:0000313|EMBL:HBC99278.1}; RN [1] {ECO:0000313|EMBL:HBC99278.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=UBA11745 {ECO:0000313|EMBL:HBC99278.1}; RX PubMed=30148503; DOI=.1038/nbt.4229; RA Parks D.H., Chuvochina M., Waite D.W., Rinke C., Skarshewski A., RA Chaumeil P.A., Hugenholtz P.; RT "A standardized bacterial taxonomy based on genome phylogeny RT substantially revises the tree of life."; RL Nat. Biotechnol. 36:996-1004(2018). CC -!- CATALYTIC ACTIVITY: CC Reaction=L-homoserine + NADP(+) = H(+) + L-aspartate 4- CC semialdehyde + NADPH; Xref=Rhea:RHEA:15761, ChEBI:CHEBI:15378, CC ChEBI:CHEBI:57476, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, CC ChEBI:CHEBI:537519; EC=1.1.1.3; CC Evidence={ECO:0000256|RuleBase:RU000579}; CC -!- PATHWAY: Amino-acid biosynthesis; L-methionine biosynthesis via de CC novo pathway; L-homoserine from L-aspartate: step 3/3. CC {ECO:0000256|RuleBase:RU000579}. CC -!- PATHWAY: Amino-acid biosynthesis; L-threonine biosynthesis; L- CC threonine from L-aspartate: step 3/5. CC {ECO:0000256|RuleBase:RU000579}. CC -!- SIMILARITY: Belongs to the homoserine dehydrogenase family. CC {ECO:0000256|RuleBase:RU004171}. CC -!- CAUTION: The sequence shown here is derived from an CC EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is CC preliminary data. {ECO:0000313|EMBL:HBC99278.1}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; DNNH01000084; HBC99278.1; -; Genomic_DNA. DR UniPathway; UPA00050; UER00063. DR UniPathway; UPA00051; UER00465. DR GO; GO:0004412; F:homoserine dehydrogenase activity; IEA:UniProtKB-EC. DR GO; GO:0050661; F:NADP binding; IEA:InterPro. DR GO; GO:0009097; P:isoleucine biosynthetic process; IEA:UniProtKB-KW. DR GO; GO:0009086; P:methionine biosynthetic process; IEA:UniProtKB-KW. DR GO; GO:0009088; P:threonine biosynthetic process; IEA:UniProtKB-UniPathway. DR InterPro; IPR005106; Asp/hSer_DH_NAD-bd. DR InterPro; IPR016204; HDH. DR InterPro; IPR001342; HDH_cat. DR InterPro; IPR019811; HDH_CS. DR InterPro; IPR036291; NAD(P)-bd_dom_sf. DR Pfam; PF00742; Homoserine_dh; 1. DR Pfam; PF03447; NAD_binding_3; 1. DR PIRSF; PIRSF000098; Homoser_dehydrog; 1. DR SUPFAM; SSF51735; SSF51735; 1. DR PROSITE; PS01042; HOMOSER_DHGENASE; 1. PE 3: Inferred from homology; KW Amino-acid biosynthesis {ECO:0000256|RuleBase:RU000579}; KW Branched-chain amino acid biosynthesis KW {ECO:0000256|RuleBase:RU000579}; KW Isoleucine biosynthesis {ECO:0000256|RuleBase:RU000579}; KW Methionine biosynthesis {ECO:0000256|RuleBase:RU000579}; KW NADP {ECO:0000256|PIRSR:PIRSR000098-2, ECO:0000256|RuleBase:RU000579}; KW Oxidoreductase {ECO:0000256|RuleBase:RU000579}; KW Threonine biosynthesis {ECO:0000256|RuleBase:RU000579}. FT DOMAIN 8 124 NAD_binding_3. {ECO:0000259|Pfam: FT PF03447}. FT DOMAIN 132 310 Homoserine_dh. {ECO:0000259|Pfam: FT PF00742}. FT NP_BIND 7 14 NADP. {ECO:0000256|PIRSR:PIRSR000098-2}. FT ACT_SITE 200 200 Proton donor. {ECO:0000256|PIRSR: FT PIRSR000098-1}. FT BINDING 100 100 NADP. {ECO:0000256|PIRSR:PIRSR000098-2}. FT BINDING 185 185 Substrate. {ECO:0000256|PIRSR: FT PIRSR000098-2}. SQ SEQUENCE 404 AA; 44446 MW; 1F2629E54473A8B7 CRC64; MRHIAVMGYG TIGSGVVEVL ERNKEIIAKR VGDEVDVKYV LDLREFPGDP AEDKIVHDFS VIEKDQSVSM VIETMGGLNP AYPFVKASLM AGKHVATSNK ALVAAYGTEL LKIAKEHNVN FFFEASVGGG IPVIRPLYTS LAGEVVEEIT GILNGTTNYI LTKMDKAGET FETALREAQE LGYAERNPEA DVDGHDTCRK IAILTAMATG HEVNYEHIYT EGITKITDVD FRYADAMGTS VKLFGSSRIQ DGTVHAFVAP VMIGKDHPLY SVNDVYNGIL VKGNMLGTSM FYGCGAGKLP TASAVVADII EALKNKDHHV EMGWDSENLT ISAMNSISFR YFVRIRGIAD KRVKEVEAVF GKVEVVELDH MDEFAVLTET MTEEDYEIKA KKLSGIRQRI RAEF //