ID A0A345FVV0_9ENTE Unreviewed; 189 AA. AC A0A345FVV0; DT 07-NOV-2018, integrated into UniProtKB/TrEMBL. DT 07-NOV-2018, sequence version 1. DT 03-MAY-2023, entry version 17. DE RecName: Full=Signal peptidase I {ECO:0000256|ARBA:ARBA00013208, ECO:0000256|RuleBase:RU003993}; DE EC=3.4.21.89 {ECO:0000256|ARBA:ARBA00013208, ECO:0000256|RuleBase:RU003993}; GN Name=lepB {ECO:0000313|EMBL:AXG39882.1}; GN ORFNames=EGCR1_14205 {ECO:0000313|EMBL:AXG39882.1}; OS Enterococcus gilvus. OG Plasmid pcr1a {ECO:0000313|Proteomes:UP000253883}. OC Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae; OC Enterococcus. OX NCBI_TaxID=160453 {ECO:0000313|EMBL:AXG39882.1, ECO:0000313|Proteomes:UP000253883}; RN [1] {ECO:0000313|EMBL:AXG39882.1, ECO:0000313|Proteomes:UP000253883} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=CR1 {ECO:0000313|EMBL:AXG39882.1, RC ECO:0000313|Proteomes:UP000253883}; RC PLASMID=pcr1a {ECO:0000313|Proteomes:UP000253883}; RA Ohki S., Hagi T., Nakano K., Shiroma A., Tamotsu H., Shimoji M., RA Shinzato M., Ashimine N., Minami M., Nakanishi T., Teruya K., Satou K., RA Moriya N., Kobayashi M., Nomura M., Suzuki C., Hirano T.; RT "Complete Genome Sequence of Carotenoid-producing Enterococcus gilvus CR1 RT Isolated from Raw Cow's Milk."; RL Submitted (JUL-2018) to the EMBL/GenBank/DDBJ databases. CC -!- CATALYTIC ACTIVITY: CC Reaction=Cleavage of hydrophobic, N-terminal signal or leader sequences CC from secreted and periplasmic proteins.; EC=3.4.21.89; CC Evidence={ECO:0000256|ARBA:ARBA00000677, CC ECO:0000256|RuleBase:RU003993}; CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000256|ARBA:ARBA00004401}; CC Single-pass type II membrane protein {ECO:0000256|ARBA:ARBA00004401}. CC Membrane {ECO:0000256|RuleBase:RU362042}; Single-pass type II membrane CC protein {ECO:0000256|RuleBase:RU362042}. CC -!- SIMILARITY: Belongs to the peptidase S26 family. CC {ECO:0000256|ARBA:ARBA00009370, ECO:0000256|RuleBase:RU362042}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; CP030933; AXG39882.1; -; Genomic_DNA. DR AlphaFoldDB; A0A345FVV0; -. DR STRING; 160453.RV02_GL002104; -. DR EnsemblBacteria; AXG39882; AXG39882; EGCR1_14205. DR KEGG; egv:EGCR1_14205; -. DR Proteomes; UP000253883; Plasmid pcr1a. DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell. DR GO; GO:0004252; F:serine-type endopeptidase activity; IEA:UniProtKB-EC. DR GO; GO:0006465; P:signal peptide processing; IEA:InterPro. DR CDD; cd06530; S26_SPase_I; 1. DR Gene3D; 2.10.109.10; Umud Fragment, subunit A; 1. DR InterPro; IPR036286; LexA/Signal_pep-like_sf. DR InterPro; IPR000223; Pept_S26A_signal_pept_1. DR InterPro; IPR019758; Pept_S26A_signal_pept_1_CS. DR InterPro; IPR019757; Pept_S26A_signal_pept_1_Lys-AS. DR InterPro; IPR019756; Pept_S26A_signal_pept_1_Ser-AS. DR InterPro; IPR019533; Peptidase_S26. DR PANTHER; PTHR43390:SF1; CHLOROPLAST PROCESSING PEPTIDASE; 1. DR PANTHER; PTHR43390; SIGNAL PEPTIDASE I; 1. DR Pfam; PF10502; Peptidase_S26; 1. DR PRINTS; PR00727; LEADERPTASE. DR SUPFAM; SSF51306; LexA/Signal peptidase; 1. DR TIGRFAMs; TIGR02227; sigpep_I_bact; 1. DR PROSITE; PS00501; SPASE_I_1; 1. DR PROSITE; PS00760; SPASE_I_2; 1. DR PROSITE; PS00761; SPASE_I_3; 1. PE 3: Inferred from homology; KW Hydrolase {ECO:0000256|RuleBase:RU003993, ECO:0000313|EMBL:AXG39882.1}; KW Membrane {ECO:0000256|RuleBase:RU003993}; KW Plasmid {ECO:0000313|EMBL:AXG39882.1}; KW Protease {ECO:0000256|ARBA:ARBA00022670, ECO:0000256|RuleBase:RU003993}; KW Transmembrane {ECO:0000256|RuleBase:RU003993}; KW Transmembrane helix {ECO:0000256|RuleBase:RU003993}. FT TRANSMEM 7..26 FT /note="Helical" FT /evidence="ECO:0000256|RuleBase:RU003993" FT DOMAIN 7..181 FT /note="Peptidase S26" FT /evidence="ECO:0000259|Pfam:PF10502" FT ACT_SITE 35 FT /evidence="ECO:0000256|PIRSR:PIRSR600223-1" FT ACT_SITE 74 FT /evidence="ECO:0000256|PIRSR:PIRSR600223-1" SQ SEQUENCE 189 AA; 21823 MW; BE8EE94FD4CE2E10 CRC64; MNNKIKEWLP VVIIIVLIFL ARMFVFSPVK VEGHSMDPTL HDSQRLITSK ISTLKRQDII TTKEPDNQNI YVVKRIIGVP GDRVAMKNNV LTINGEEVPE PYLDDFKEKF KKDKLAEEYS YSTAFQEQAA NATSFTNDFD VTVPNNQYFV LGDNRLISKD SRIFGFVDKS LIQGKVVLRF WPLDKFKLF //