ID A0A317SC64_9PEZI Unreviewed; 2125 AA. AC A0A317SC64; DT 10-OCT-2018, integrated into UniProtKB/TrEMBL. DT 10-OCT-2018, sequence version 1. DT 11-DEC-2019, entry version 8. DE RecName: Full=DNA polymerase epsilon catalytic subunit {ECO:0000256|RuleBase:RU365029}; DE EC=2.7.7.7 {ECO:0000256|RuleBase:RU365029}; GN ORFNames=C7212DRAFT_354896 {ECO:0000313|EMBL:PWW72129.1}; OS Tuber magnatum (white Piedmont truffle). OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Pezizomycetes; OC Pezizales; Tuberaceae; Tuber. OX NCBI_TaxID=42249 {ECO:0000313|EMBL:PWW72129.1, ECO:0000313|Proteomes:UP000246991}; RN [1] {ECO:0000313|EMBL:PWW72129.1, ECO:0000313|Proteomes:UP000246991} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=091103-1 {ECO:0000313|EMBL:PWW72129.1}; RA Murat C., Payen T., Noel B., Kuo A., Martin F.M.; RT "Genomes of Pezizomycetes fungi and the evolution of truffles."; RL Submitted (MAR-2018) to the EMBL/GenBank/DDBJ databases. CC -!- FUNCTION: DNA polymerase II participates in chromosomal DNA CC replication. {ECO:0000256|RuleBase:RU365029}. CC -!- CATALYTIC ACTIVITY: CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) = CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:11130, CC Rhea:RHEA-COMP:11131, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560, CC ChEBI:CHEBI:83828; EC=2.7.7.7; CC Evidence={ECO:0000256|RuleBase:RU365029}; CC -!- COFACTOR: CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; CC Evidence={ECO:0000256|RuleBase:RU365029}; CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000256|RuleBase:RU365029}. CC -!- SIMILARITY: Belongs to the DNA polymerase type-B family. CC {ECO:0000256|RuleBase:RU365029}. CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ CC whole genome shotgun (WGS) entry which is preliminary data. CC {ECO:0000313|EMBL:PWW72129.1}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; PYWC01000117; PWW72129.1; -; Genomic_DNA. DR Proteomes; UP000246991; Unassembled WGS sequence. DR GO; GO:0008622; C:epsilon DNA polymerase complex; IEA:InterPro. DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW. DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW. DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW. DR GO; GO:0000166; F:nucleotide binding; IEA:InterPro. DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro. DR GO; GO:0006281; P:DNA repair; IEA:InterPro. DR GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW. DR Gene3D; 1.10.132.60; -; 1. DR Gene3D; 3.30.420.10; -; 1. DR Gene3D; 3.90.1600.10; -; 1. DR InterPro; IPR006172; DNA-dir_DNA_pol_B. DR InterPro; IPR006133; DNA-dir_DNA_pol_B_exonuc. DR InterPro; IPR006134; DNA-dir_DNA_pol_B_multi_dom. DR InterPro; IPR042087; DNA_pol_B_C. DR InterPro; IPR013697; DNA_pol_e_suA_C. DR InterPro; IPR023211; DNA_pol_palm_dom_sf. DR InterPro; IPR029703; POL2. DR InterPro; IPR012337; RNaseH-like_sf. DR InterPro; IPR036397; RNaseH_sf. DR PANTHER; PTHR10670; PTHR10670; 3. DR Pfam; PF00136; DNA_pol_B; 1. DR Pfam; PF03104; DNA_pol_B_exo1; 1. DR Pfam; PF08490; DUF1744; 1. DR SMART; SM01159; DUF1744; 1. DR SMART; SM00486; POLBc; 1. DR SUPFAM; SSF53098; SSF53098; 1. PE 3: Inferred from homology; KW 4Fe-4S {ECO:0000256|RuleBase:RU365029}; KW Coiled coil {ECO:0000256|SAM:Coils}; KW DNA replication {ECO:0000256|RuleBase:RU365029}; KW DNA-binding {ECO:0000256|RuleBase:RU365029}; KW DNA-directed DNA polymerase {ECO:0000256|RuleBase:RU365029}; KW Iron {ECO:0000256|RuleBase:RU365029}; KW Iron-sulfur {ECO:0000256|RuleBase:RU365029}; KW Metal-binding {ECO:0000256|RuleBase:RU365029}; KW Nucleotidyltransferase {ECO:0000256|RuleBase:RU365029}; KW Nucleus {ECO:0000256|RuleBase:RU365029}; KW Reference proteome {ECO:0000313|Proteomes:UP000246991}; KW Transferase {ECO:0000256|RuleBase:RU365029}; KW Zinc {ECO:0000256|RuleBase:RU365029}; KW Zinc-finger {ECO:0000256|RuleBase:RU365029}. FT DOMAIN 1418..1811 FT /note="DUF1744" FT /evidence="ECO:0000259|SMART:SM01159" FT REGION 1..30 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT REGION 666..686 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT COILED 609..629 FT /evidence="ECO:0000256|SAM:Coils" FT COILED 1458..1478 FT /evidence="ECO:0000256|SAM:Coils" SQ SEQUENCE 2125 AA; 242335 MW; 68F9DB14E2BC5348 CRC64; MYSSRGSSGS TRFKRGSYRR GGNSGGGRFQ KTKTITANSL LATDGTSGEA RFESLRLAND IDEQMGFARF EAGPKRVGWL VNMKSTILED DQVTGGKAGV DFYFLDDYGE SFKATVLYDP YFLIATKRGN EPEVEEWLRR KFEGIIKKTS RLMKEDLQMP NHLLGYRRTF IKLEFANVTD LLAARKVLMP IAEKNRKNVN AMDAYAEVAS ASAGFDFIDE GNDVRKSTNM AEACDFIIDI REYDVPYHVR VTIDQGIRIG KWYTVEAKHG IVSLTCIEDR LKQAEPVVLA WDIETTKLPL KFPDASIDQI MMISYMIEGS GFLITNREIV SQDIEDFEYT PRPEYEGHFM VFNEPDEKAV LERFFDHIKE AKPTVMVTYN GDFFDWPFVE ARASQLGIDM YREIGFRKNS EDVYYSNYSA HMDAFAWVKR DSYLPQGSQG LKAVTTAKLG YDPDELDPEL MTRYAVEKPQ VLAEYSVSDA VATYYLYMKY VHPFVFALCT IIPLGPDDVL RKGTGTLCEM LLMVQAYRKE IVLPNKHKQV EEAFYDGHLL GSETYVGGHV ESIQAGVFRS DIPADFVIDT KAIDKLIEDL DSALEFSIVV EGKKKMEDVT NYDEVKEQIR SKLQQLKDVP TRNERPSIYH LDVASIGEYL PAKKDEYNMI RRALENEKFP PKNPKSPPRT WDDLASEEQA SAIKKRLADY SKKVYHKIHE TKTIEKEAII CQRENPFYVD TVKNFRDRRY DFKGKQKVWK RKADDLKASG ATAAEIDEAN KMTVLFDSMQ LAHKVILNSF YGYVMRKGSR WYSMEMAGVT CLTGAHIIQM AKEIVEQIGR PLELDTDGIW CILPGTFPEN FAFTLGGGRK LPISYPCVML NHLVHAKFTN HQYAELVDPV THYYTTHDEN TIFFEVDGPY RAMILPTSKE EDKNLKKRYA VFNHDGSLAE LKGFEVKRRG ELKLIKIFQS QIFKFFLEGT SLEECYGAVA KTANRWLDVL HSRGSTLADE ELIDLICENR SMSKTLAEYG SQKSTSITTA KRLADFLGEQ MVKDKGLNCK YIVSAKPRGA PKVRNPVPRV PHPDWLQRRI DIEDDRFKQK KMTDMFQKRT ELEIVPLGVP THLEDIEDFS GTTTMPINRP ILGTAKRLGK RKAPDTPAPV GGDPFASLPA IMPSITEDYR GWLQYQKKKW KIQKHARTRR RQLFGDRSSL QADSIGGFFR NQTEFMLSNT WEILQLRPTD MPGEVRAFVL VGRKIITLKI KVPRQIFINF KNEELPDVTV QGCIVERVNH TLPNGHPSGN LFKLVVPEDT YLSEQKAFSI TFSHASVEGV YETQVPMSIR VLLELGNSCT FDESQRGVLG KGLDQGFALS SLKSKETKSP YLQIGGMDYL FLYHVVAGDR QVFGLFPPGG EAHVIVYNKT REVQGLPNMD KIYAEQFIRR MEAANGPRDS MIEYPATLHF QTTYVNTKKK LEKGLTELLK KIRSEESKPT TLVIQSPQRR TLVDTMSILN EFPILALRSD PSDSNLPPLG WQTFVAKRIV THYLASGAWI LHLVELARYG RIPLCNLEKD DPTFLIDIDY ARRLQKNNVV LWWSPSPRPD HGGHEKDDIL GSMETVDMPA INNPGSYSSV CIEIEVRNLA INTILTSSLI NELEGSDSVA MNPAAPQGDD PADGSNIIFA NNAFATAGVA VLREMVKAWS ALATRGQPVA EIMVEHLVRW VESPDSFLYD RALHYYVQTM SKKAFLQLLT DFRRVGSHVV FANVNKLLIQ TTKVDVGNAY AYSEYIIKAI RAKPLFNFLD LKINEYWDYL VWYDEMNHGG KACEKVVEVE NQELNVIMHW QFAKFLPQTL QTIFHDWVVE FVEIMHTQKN PPASRSDTST PRPTQIFFKM SEDSGDKIAL GSEFKMPLRK QIGQLIRRQQ ESMIHPEINV DFEFPSLPGA YREMKNPVLQ LVRSLTAVFG LAKEINIEVR TFRKELLAML DIKEFSKDGT FENPSETLKF SQVICKNCAL PRDMDLCKDE NLMPEVTADG QLRERAWKCK FDFCGSEFDR LAFEEKMISQ LHQFVTAYNV QDLKCGKCQT LKVNEFMEHC SCSGPWVLTV EREVLIKRLH VYKSVAEHYS FRMLEGVVGD IMGCV //