ID A0A2Z5UY12_9EUCA Unreviewed; 513 AA. AC A0A2Z5UY12; DT 10-OCT-2018, integrated into UniProtKB/TrEMBL. DT 10-OCT-2018, sequence version 1. DT 16-JAN-2019, entry version 4. DE RecName: Full=NADH-ubiquinone oxidoreductase chain 4 {ECO:0000256|RuleBase:RU003297}; DE EC=7.1.1.2 {ECO:0000256|RuleBase:RU003297}; GN Name=ND4 {ECO:0000313|EMBL:BBB16292.1}; OS Pagurus gracilipes. OG Mitochondrion {ECO:0000313|EMBL:BBB16292.1}. OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Crustacea; Multicrustacea; OC Malacostraca; Eumalacostraca; Eucarida; Decapoda; Pleocyemata; OC Anomura; Paguroidea; Paguridae; Pagurus. OX NCBI_TaxID=1929474 {ECO:0000313|EMBL:BBB16292.1}; RN [1] {ECO:0000313|EMBL:BBB16292.1} RP NUCLEOTIDE SEQUENCE. RA Sultana Z., Asakura A., Kinjo S., Nozawa M., Nakano T., Ikeo K.; RT "Molecular phylogeny of ten intertidal hermit crabs of the genus RT Pagurus inferred from multiple mitochondrial genes, with special RT emphasis on the evolutionary relationship of Pagurus lanuginosus and RT Pagurus maculosus."; RL Genetica 0:0-0(2017). CC -!- FUNCTION: Core subunit of the mitochondrial membrane respiratory CC chain NADH dehydrogenase (Complex I) that is believed to belong to CC the minimal assembly required for catalysis. Complex I functions CC in the transfer of electrons from NADH to the respiratory chain. CC The immediate electron acceptor for the enzyme is believed to be CC ubiquinone. {ECO:0000256|RuleBase:RU003297}. CC -!- CATALYTIC ACTIVITY: CC Reaction=a ubiquinone + 5 H(+)(in) + NADH = a ubiquinol + 4 CC H(+)(out) + NAD(+); Xref=Rhea:RHEA:29091, Rhea:RHEA-COMP:9565, CC Rhea:RHEA-COMP:9566, ChEBI:CHEBI:15378, ChEBI:CHEBI:16389, CC ChEBI:CHEBI:17976, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; CC EC=7.1.1.2; Evidence={ECO:0000256|RuleBase:RU003297}; CC -!- SUBCELLULAR LOCATION: Mitochondrion membrane CC {ECO:0000256|RuleBase:RU003297}; Multi-pass membrane protein CC {ECO:0000256|RuleBase:RU003297}. CC -!- SIMILARITY: Belongs to the complex I subunit 4 family. CC {ECO:0000256|RuleBase:RU003297, ECO:0000256|SAAS:SAAS00707377}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; LC222534; BBB16292.1; -; Genomic_DNA. DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW. DR GO; GO:0005739; C:mitochondrion; IEA:UniProtKB-KW. DR GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; IEA:UniProtKB-UniRule. DR GO; GO:0006120; P:mitochondrial electron transport, NADH to ubiquinone; IEA:InterPro. DR InterPro; IPR000260; NADH4_N. DR InterPro; IPR003918; NADH_UbQ_OxRdtase. DR InterPro; IPR001750; ND/Mrp_mem. DR PANTHER; PTHR43507; PTHR43507; 1. DR Pfam; PF01059; Oxidored_q5_N; 1. DR Pfam; PF00361; Proton_antipo_M; 1. DR PRINTS; PR01437; NUOXDRDTASE4. PE 3: Inferred from homology; KW Electron transport {ECO:0000256|RuleBase:RU003297}; KW Membrane {ECO:0000256|RuleBase:RU003297, KW ECO:0000256|SAAS:SAAS00707373}; KW Mitochondrion {ECO:0000256|RuleBase:RU003297, KW ECO:0000313|EMBL:BBB16292.1}; KW NAD {ECO:0000256|RuleBase:RU003297, ECO:0000256|SAAS:SAAS00707374}; KW Respiratory chain {ECO:0000256|RuleBase:RU003297}; KW Transmembrane {ECO:0000256|RuleBase:RU003297, KW ECO:0000256|SAAS:SAAS00707385}; KW Transmembrane helix {ECO:0000256|RuleBase:RU003297, KW ECO:0000256|SAAS:SAAS00707361}; KW Transport {ECO:0000256|RuleBase:RU003297}; KW Ubiquinone {ECO:0000256|RuleBase:RU003297}. FT TRANSMEM 53 70 Helical. {ECO:0000256|RuleBase:RU003297}. FT TRANSMEM 82 101 Helical. {ECO:0000256|RuleBase:RU003297}. FT TRANSMEM 107 129 Helical. {ECO:0000256|RuleBase:RU003297}. FT TRANSMEM 141 161 Helical. {ECO:0000256|RuleBase:RU003297}. FT TRANSMEM 181 204 Helical. {ECO:0000256|RuleBase:RU003297}. FT TRANSMEM 216 235 Helical. {ECO:0000256|RuleBase:RU003297}. FT TRANSMEM 247 266 Helical. {ECO:0000256|RuleBase:RU003297}. FT TRANSMEM 273 295 Helical. {ECO:0000256|RuleBase:RU003297}. FT TRANSMEM 301 322 Helical. {ECO:0000256|RuleBase:RU003297}. FT TRANSMEM 343 361 Helical. {ECO:0000256|RuleBase:RU003297}. FT TRANSMEM 381 401 Helical. {ECO:0000256|RuleBase:RU003297}. FT TRANSMEM 422 443 Helical. {ECO:0000256|RuleBase:RU003297}. FT TRANSMEM 481 501 Helical. {ECO:0000256|RuleBase:RU003297}. FT DOMAIN 1 100 Oxidored_q5_N. {ECO:0000259|Pfam: FT PF01059}. FT DOMAIN 103 390 Proton_antipo_M. {ECO:0000259|Pfam: FT PF00361}. SQ SEQUENCE 513 AA; 58227 MW; 991703CE987DB843 CRC64; MLKFILALMM LMIFIGEWNL IQVGLFILCF LLNIFSCHDF YMSNLGFGLG SDYLGYILIL LSAWIMSLVI SSSTKVMFNN IFPSSFLIVN ILLLVALFVT FSSMDYLMFY ISFEASLIPT LILILGWGYQ PERIQAGVYM LFYTLLASLP LLVSILYLYK INGSLSMGLN YNMLNVSSMN LIWFVCSMMA FVVKLPMYMF HLWLPKAHVE APVAGSMILA GVLLKLGGYG LIRILPLFSE LNKSINWVWI GLSILGGFIV SLICLRQVDM KSLIAYSSVA HMGLVLSGLV MFGWWGLNGA VVVMVGHGLC SSGMFCLANM VYERMGSRSL LINKGLMNFM PSMALWWFLL SVGNMAAPPT INLLGEINLI MSMVSWSKLT MVGICLLSFF SAAYTLYLYS LSQHGLFSSS LYSCCSGKVR EYLVLSLHWI PLNILILKSI LIMPNFYLNS LMKTLVCGTK NMNMVILNHH MKNINQLKQN IIFIISIFIF YNNIFILYFI WKMLYYWMGN FIY //