ID UNGB_ASPV1 Reviewed; 547 AA. AC A0A2V5HGL3; DT 08-NOV-2023, integrated into UniProtKB/Swiss-Prot. DT 12-SEP-2018, sequence version 1. DT 08-NOV-2023, entry version 20. DE RecName: Full=MFS-type transporter ungB {ECO:0000303|PubMed:36715406}; DE AltName: Full=Unguisins biosynthesis cluster protein B {ECO:0000303|PubMed:36715406}; GN Name=ungB {ECO:0000303|PubMed:36715406}; ORFNames=BO99DRAFT_343333; OS Aspergillus violaceofuscus (strain CBS 115571). OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes; OC Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus. OX NCBI_TaxID=1450538; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=CBS 115571; RG DOE Joint Genome Institute; RA Vesth T.C., Nybo J., Theobald S., Brandl J., Frisvad J.C., Nielsen K.F., RA Lyhne E.K., Kogle M.E., Kuo A., Riley R., Clum A., Nolan M., Lipzen A., RA Salamov A., Henrissat B., Wiebenga A., De vries R.P., Grigoriev I.V., RA Mortensen U.H., Andersen M.R., Baker S.E.; RT "The genomes of Aspergillus section Nigri reveals drivers in fungal RT speciation."; RL Submitted (FEB-2018) to the EMBL/GenBank/DDBJ databases. RN [2] RP FUNCTION. RX PubMed=36715406; DOI=10.1021/acs.jnatprod.2c01065; RA Wei X., Chan T.K., Kong C.T.D., Matsuda Y.; RT "Biosynthetic characterization, heterologous production, and genomics- RT guided discovery of GABA-containing fungal heptapeptides."; RL J. Nat. Prod. 86:416-422(2023). CC -!- FUNCTION: MFS-type transporter; part of the gene cluster that mediates CC the biosynthesis of the unguisins, gamma-aminobutyric acid (GABA)- CC containing fungal cyclic heptapeptides with the amino acid sequence CC cyclo-(D-Ala1-D-Val2-L-Phe3-D-Val4-D-Ala5-D-Trp6-GABA7) for unguisin A CC and cyclo-(D-Ala1-D-Val2-L-Leu3-D-Val4-D-Ala5-D-Trp6-GABA7) for CC unguisin B (PubMed:36715406). May be involved in the secretion of CC unguisins (Probable). {ECO:0000269|PubMed:36715406, CC ECO:0000305|PubMed:36715406}. CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Multi-pass membrane CC protein {ECO:0000255}. CC -!- SIMILARITY: Belongs to the major facilitator superfamily. TCR/Tet CC family. {ECO:0000305}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; KZ825194; PYI15150.1; -; Genomic_DNA. DR AlphaFoldDB; A0A2V5HGL3; -. DR OMA; WDDWRIR; -. DR Proteomes; UP000249829; Unassembled WGS sequence. DR GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell. DR GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro. DR CDD; cd17502; MFS_Azr1_MDR_like; 1. DR Gene3D; 1.20.1250.20; MFS general substrate transporter like domains; 1. DR Gene3D; 1.20.1720.10; Multidrug resistance protein D; 1. DR InterPro; IPR011701; MFS. DR InterPro; IPR020846; MFS_dom. DR InterPro; IPR036259; MFS_trans_sf. DR PANTHER; PTHR23501:SF147; AZOLE RESISTANCE PROTEIN 1-RELATED; 1. DR PANTHER; PTHR23501; MAJOR FACILITATOR SUPERFAMILY; 1. DR Pfam; PF07690; MFS_1; 1. DR PRINTS; PR01036; TCRTETB. DR SUPFAM; SSF103473; MFS general substrate transporter; 1. DR PROSITE; PS50850; MFS; 1. PE 3: Inferred from homology; KW Membrane; Reference proteome; Transmembrane; Transmembrane helix; KW Transport. FT CHAIN 1..547 FT /note="MFS-type transporter ungB" FT /id="PRO_5016020346" FT TRANSMEM 14..34 FT /note="Helical" FT /evidence="ECO:0000255" FT TRANSMEM 50..70 FT /note="Helical" FT /evidence="ECO:0000255" FT TRANSMEM 80..100 FT /note="Helical" FT /evidence="ECO:0000255" FT TRANSMEM 111..131 FT /note="Helical" FT /evidence="ECO:0000255" FT TRANSMEM 138..158 FT /note="Helical" FT /evidence="ECO:0000255" FT TRANSMEM 169..189 FT /note="Helical" FT /evidence="ECO:0000255" FT TRANSMEM 210..230 FT /note="Helical" FT /evidence="ECO:0000255" FT TRANSMEM 238..258 FT /note="Helical" FT /evidence="ECO:0000255" FT TRANSMEM 279..299 FT /note="Helical" FT /evidence="ECO:0000255" FT TRANSMEM 316..336 FT /note="Helical" FT /evidence="ECO:0000255" FT TRANSMEM 343..363 FT /note="Helical" FT /evidence="ECO:0000255" FT TRANSMEM 366..386 FT /note="Helical" FT /evidence="ECO:0000255" FT TRANSMEM 392..412 FT /note="Helical" FT /evidence="ECO:0000255" FT TRANSMEM 475..495 FT /note="Helical" FT /evidence="ECO:0000255" SQ SEQUENCE 547 AA; 57751 MW; E11902F7506EBB1D CRC64; MEDEPTYPSF RQSLLVTISL CLTLFCVSLD ETVLATAIPR ITDQFQSLND VGWYGSSYLF VFTATQMAWG KLYTMYPAKW VFLTGVTVFE VGSLVCGVSP TSGALIAGRS IAGLGAGSIN AGAVLIISNT IPVQKRPIYL GCLGVVHGVV SVLGPVIGGL LTDHASWRWC FFLNLPIGAI TVLGIVFCLS TNQPSAGHLS GKEKLRSMDL LGSAFFIPGI LMLLLALEWG GSQYAWDSWR VILLFVLSAV ALAVFAVVQV RAPEKATIAP RLVTNRNMLG LIGYIVGNSG GLFVFVYYLP IWLQAIKEFS ASKSGLAILP TQLGMVAASL AGGILVTVVR YYTPFLIVSS LLAVAGAGLL SSLHPASGLG SILGYQVVLS VGIGLGAQNA MVVPSVVCAP GDVVMAIATL CFLQMLSSSI ALTLAQTVFH SRLVTNLAHR APSVDSALVE QGATRLRDRV PADLLPSVVG AYSQAVSETF YVGVAMCALS LLGSASMQWK RVPGHKEATE KVEGEGQGQG QQQEQDQGQG WGEVGESHAL AHPTADK //