ID A0A2U8FZY0_RHIFR Unreviewed; 220 AA. AC A0A2U8FZY0; DT 12-SEP-2018, integrated into UniProtKB/TrEMBL. DT 12-SEP-2018, sequence version 1. DT 14-DEC-2022, entry version 11. DE RecName: Full=(S)-2-haloacid dehalogenase {ECO:0000256|RuleBase:RU368077}; DE EC=3.8.1.2 {ECO:0000256|RuleBase:RU368077}; DE AltName: Full=2-haloalkanoic acid dehalogenase {ECO:0000256|RuleBase:RU368077}; DE AltName: Full=Halocarboxylic acid halidohydrolase {ECO:0000256|RuleBase:RU368077}; DE AltName: Full=L-2-haloacid dehalogenase {ECO:0000256|RuleBase:RU368077}; GN ORFNames=AB395_0000660 {ECO:0000313|EMBL:AWI56338.1}; OS Sinorhizobium fredii CCBAU 45436. OC Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; OC Rhizobiaceae; Sinorhizobium/Ensifer group; Sinorhizobium. OX NCBI_TaxID=1128331 {ECO:0000313|EMBL:AWI56338.1, ECO:0000313|Proteomes:UP000244927}; RN [1] {ECO:0000313|EMBL:AWI56338.1, ECO:0000313|Proteomes:UP000244927} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=CCBAU 45436 {ECO:0000313|EMBL:AWI56338.1, RC ECO:0000313|Proteomes:UP000244927}; RA Lanie J.A., Ng W.-L., Kazmierczak K.M., Andrzejewski T.M., Davidsen T.M., RA Wayne K.J., Tettelin H., Glass J.I., Rusch D., Podicherti R., Tsui H.-C.T., RA Winkler M.E.; RL Submitted (MAY-2018) to the EMBL/GenBank/DDBJ databases. CC -!- FUNCTION: Catalyzes the hydrolytic dehalogenation of small (S)-2- CC haloalkanoic acids to yield the corresponding (R)-2-hydroxyalkanoic CC acids. {ECO:0000256|RuleBase:RU368077}. CC -!- CATALYTIC ACTIVITY: CC Reaction=an (S)-2-haloacid + H2O = a (2R)-2-hydroxycarboxylate + a CC halide anion + H(+); Xref=Rhea:RHEA:11192, ChEBI:CHEBI:15377, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:16042, ChEBI:CHEBI:58314, CC ChEBI:CHEBI:137405; EC=3.8.1.2; CC Evidence={ECO:0000256|RuleBase:RU368077}; CC -!- SIMILARITY: Belongs to the HAD-like hydrolase superfamily. S-2- CC haloalkanoic acid dehalogenase family. {ECO:0000256|ARBA:ARBA00008106, CC ECO:0000256|RuleBase:RU368077}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; CP029231; AWI56338.1; -; Genomic_DNA. DR RefSeq; WP_014327673.1; NZ_CP029231.1. DR AlphaFoldDB; A0A2U8FZY0; -. DR EnsemblBacteria; AWI56338; AWI56338; AB395_0000660. DR GeneID; 48972266; -. DR Proteomes; UP000244927; Chromosome. DR GO; GO:0018784; F:(S)-2-haloacid dehalogenase activity; IEA:UniProtKB-UniRule. DR CDD; cd02588; HAD_L2-DEX; 1. DR Gene3D; 1.10.150.240; -; 1. DR Gene3D; 3.40.50.1000; -; 1. DR InterPro; IPR006328; 2-HAD. DR InterPro; IPR036412; HAD-like_sf. DR InterPro; IPR006439; HAD-SF_hydro_IA. DR InterPro; IPR041492; HAD_2. DR InterPro; IPR023214; HAD_sf. DR InterPro; IPR023198; PGP-like_dom2. DR Pfam; PF13419; HAD_2; 1. DR PRINTS; PR00413; HADHALOGNASE. DR SUPFAM; SSF56784; HAD-like; 1. DR TIGRFAMs; TIGR01493; HAD-SF-IA-v2; 1. DR TIGRFAMs; TIGR01428; HAD_type_II; 1. PE 3: Inferred from homology; KW Hydrolase {ECO:0000256|RuleBase:RU368077}. SQ SEQUENCE 220 AA; 24554 MW; E46A2B5ECB3AAC15 CRC64; MSHAAYVFDA YGTLFDVHAA VRRHAEAIGP DGQAFSELWR AKQLEYSWVR SLMGAYVDFW QLTEQSLDYA FARFPSADPK LRKPLLDAYW ALDCYPEVPA VLKGLKERGA RIAILSNGSP AMLASAVKNA ALDIIIDDVF SVDAVKAFKT APAVYDLVTT QYRLYPHAVS FQSSNRFDIA GATKFGFRTV WINRADMPDE YKDYGPSLIL PSLESLQIGI //