ID A0A2U3MRJ0_LEUCA Unreviewed; 306 AA. AC A0A2U3MRJ0; DT 18-JUL-2018, integrated into UniProtKB/TrEMBL. DT 18-JUL-2018, sequence version 1. DT 03-MAY-2023, entry version 19. DE RecName: Full=Pseudouridine synthase {ECO:0000256|RuleBase:RU362028}; DE EC=5.4.99.- {ECO:0000256|RuleBase:RU362028}; GN ORFNames=LCAC16_40010 {ECO:0000313|EMBL:SPJ43761.1}; OS Leuconostoc carnosum. OC Bacteria; Bacillota; Bacilli; Lactobacillales; Lactobacillaceae; OC Leuconostoc. OX NCBI_TaxID=1252 {ECO:0000313|EMBL:SPJ43761.1, ECO:0000313|Proteomes:UP000295336}; RN [1] {ECO:0000313|Proteomes:UP000295336} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=MFPC16A2803 {ECO:0000313|Proteomes:UP000295336}; RA Chaillou S.; RL Submitted (MAR-2018) to the EMBL/GenBank/DDBJ databases. CC -!- FUNCTION: Responsible for synthesis of pseudouridine from uracil. CC {ECO:0000256|RuleBase:RU362028}. CC -!- CATALYTIC ACTIVITY: CC Reaction=a uridine in RNA = a pseudouridine in RNA; CC Xref=Rhea:RHEA:48348, Rhea:RHEA-COMP:12068, Rhea:RHEA-COMP:12069, CC ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; CC Evidence={ECO:0000256|RuleBase:RU362028}; CC -!- SIMILARITY: Belongs to the pseudouridine synthase RluA family. CC {ECO:0000256|ARBA:ARBA00010876, ECO:0000256|RuleBase:RU362028}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; ONZM01000024; SPJ43761.1; -; Genomic_DNA. DR AlphaFoldDB; A0A2U3MRJ0; -. DR EnsemblBacteria; SPJ43761; SPJ43761; LCAC16_40010. DR OrthoDB; 9773999at2; -. DR Proteomes; UP000295336; Unassembled WGS sequence. DR GO; GO:0140098; F:catalytic activity, acting on RNA; IEA:UniProt. DR GO; GO:0009982; F:pseudouridine synthase activity; IEA:InterPro. DR GO; GO:0003723; F:RNA binding; IEA:InterPro. DR GO; GO:0034470; P:ncRNA processing; IEA:UniProt. DR GO; GO:0001522; P:pseudouridine synthesis; IEA:InterPro. DR CDD; cd02869; PseudoU_synth_RluA_like; 1. DR Gene3D; 3.30.2350.10; Pseudouridine synthase; 1. DR InterPro; IPR020103; PsdUridine_synth_cat_dom_sf. DR InterPro; IPR006224; PsdUridine_synth_RluA-like_CS. DR InterPro; IPR006225; PsdUridine_synth_RluC/D. DR InterPro; IPR006145; PsdUridine_synth_RsuA/RluA. DR PANTHER; PTHR21600:SF71; LARGE SUBUNIT PSEUDOURIDINE SYNTHASE, RLUD SUBFAMILY, PUTATIVE-RELATED; 1. DR PANTHER; PTHR21600; MITOCHONDRIAL RNA PSEUDOURIDINE SYNTHASE; 1. DR Pfam; PF00849; PseudoU_synth_2; 1. DR SUPFAM; SSF55120; Pseudouridine synthase; 1. DR TIGRFAMs; TIGR00005; rluA_subfam; 1. DR PROSITE; PS01129; PSI_RLU; 1. PE 3: Inferred from homology; KW Isomerase {ECO:0000256|RuleBase:RU362028, ECO:0000313|EMBL:SPJ43761.1}. FT DOMAIN 94..251 FT /note="Pseudouridine synthase RsuA/RluA-like" FT /evidence="ECO:0000259|Pfam:PF00849" FT ACT_SITE 146 FT /evidence="ECO:0000256|PIRSR:PIRSR606225-1" SQ SEQUENCE 306 AA; 34729 MW; 560DB0D3DEDEF6BE CRC64; MMTSWHYQIT VCDGQVGKTI KALFISWLIP QRIRGALRMK QHFRVNDHEV PTNYVLKTGD VLTLLFENDD FRTSESHYLP NDKQQVAIVF ENDDFVIVDK PAGMKMHPHS ITETDTLLNY LAANFQMRQL LSASAQAKPY MVHRIDRETS GLVIVAKNPV VVPILNRLLS EKIIKRTYLA WVTGAMGERN GSINVPIGVD FLNDRKRWIN GIDAQTAITH WTCIHTVYQN SLLRLQLETG RMHQIRVHLA SVGHPIIGDS LYGSQSEATR MMLHSVSIEL PLPFDGGHKI ITGALPVDFP RQLMID //