ID A0A2U3MRJ0_LEUCA Unreviewed; 306 AA. AC A0A2U3MRJ0; DT 18-JUL-2018, integrated into UniProtKB/TrEMBL. DT 18-JUL-2018, sequence version 1. DT 12-AUG-2020, entry version 12. DE RecName: Full=Pseudouridine synthase {ECO:0000256|RuleBase:RU362028}; DE EC=5.4.99.- {ECO:0000256|RuleBase:RU362028}; GN ORFNames=LCAC16_40010 {ECO:0000313|EMBL:SPJ43761.1}; OS Leuconostoc carnosum. OC Bacteria; Firmicutes; Bacilli; Lactobacillales; Leuconostocaceae; OC Leuconostoc. OX NCBI_TaxID=1252 {ECO:0000313|EMBL:SPJ43761.1, ECO:0000313|Proteomes:UP000295336}; RN [1] {ECO:0000313|Proteomes:UP000295336} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=MFPC16A2803 {ECO:0000313|Proteomes:UP000295336}; RA Chaillou S.; RL Submitted (MAR-2018) to the EMBL/GenBank/DDBJ databases. CC -!- FUNCTION: Responsible for synthesis of pseudouridine from uracil. CC {ECO:0000256|RuleBase:RU362028}. CC -!- CATALYTIC ACTIVITY: CC Reaction=a uridine in RNA = a pseudouridine in RNA; CC Xref=Rhea:RHEA:48348, Rhea:RHEA-COMP:12068, Rhea:RHEA-COMP:12069, CC ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; CC Evidence={ECO:0000256|RuleBase:RU362028}; CC -!- SIMILARITY: Belongs to the pseudouridine synthase RluA family. CC {ECO:0000256|ARBA:ARBA00010876, ECO:0000256|RuleBase:RU362028}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; ONZM01000024; SPJ43761.1; -; Genomic_DNA. DR Proteomes; UP000295336; Unassembled WGS sequence. DR GO; GO:0009982; F:pseudouridine synthase activity; IEA:InterPro. DR GO; GO:0003723; F:RNA binding; IEA:InterPro. DR GO; GO:0001522; P:pseudouridine synthesis; IEA:InterPro. DR CDD; cd02869; PseudoU_synth_RluCD_like; 1. DR InterPro; IPR020103; PsdUridine_synth_cat_dom_sf. DR InterPro; IPR006225; PsdUridine_synth_RluC/D. DR InterPro; IPR006224; PsdUridine_synth_RluC/D_CS. DR InterPro; IPR006145; PsdUridine_synth_RsuA/RluA. DR Pfam; PF00849; PseudoU_synth_2; 1. DR SUPFAM; SSF55120; SSF55120; 1. DR TIGRFAMs; TIGR00005; rluA_subfam; 1. DR PROSITE; PS01129; PSI_RLU; 1. PE 3: Inferred from homology; KW Isomerase {ECO:0000256|RuleBase:RU362028, ECO:0000313|EMBL:SPJ43761.1}. FT DOMAIN 94..251 FT /note="PseudoU_synth_2" FT /evidence="ECO:0000259|Pfam:PF00849" FT ACT_SITE 146 FT /evidence="ECO:0000256|PIRSR:PIRSR606225-1" SQ SEQUENCE 306 AA; 34729 MW; 560DB0D3DEDEF6BE CRC64; MMTSWHYQIT VCDGQVGKTI KALFISWLIP QRIRGALRMK QHFRVNDHEV PTNYVLKTGD VLTLLFENDD FRTSESHYLP NDKQQVAIVF ENDDFVIVDK PAGMKMHPHS ITETDTLLNY LAANFQMRQL LSASAQAKPY MVHRIDRETS GLVIVAKNPV VVPILNRLLS EKIIKRTYLA WVTGAMGERN GSINVPIGVD FLNDRKRWIN GIDAQTAITH WTCIHTVYQN SLLRLQLETG RMHQIRVHLA SVGHPIIGDS LYGSQSEATR MMLHSVSIEL PLPFDGGHKI ITGALPVDFP RQLMID //