ID A0A2T3DEL7_9RHIZ Unreviewed; 416 AA. AC A0A2T3DEL7; DT 18-JUL-2018, integrated into UniProtKB/TrEMBL. DT 18-JUL-2018, sequence version 1. DT 11-DEC-2019, entry version 7. DE SubName: Full=Class I SAM-dependent RNA methyltransferase {ECO:0000313|EMBL:PSS67205.1}; GN ORFNames=C6558_04125 {ECO:0000313|EMBL:PSS67205.1}; OS Ensifer sp. NM-2. OC Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales; Rhizobiaceae; OC Sinorhizobium/Ensifer group; Ensifer; unclassified Ensifer. OX NCBI_TaxID=2109730 {ECO:0000313|EMBL:PSS67205.1, ECO:0000313|Proteomes:UP000240263}; RN [1] {ECO:0000313|EMBL:PSS67205.1, ECO:0000313|Proteomes:UP000240263} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=NM-2 {ECO:0000313|EMBL:PSS67205.1, RC ECO:0000313|Proteomes:UP000240263}; RA Lund M.K., Andersen N., Dalskov L., Marshall I.P.G., Finster K., RA Schramm A.; RT "Genome sequence of Ensifer ninsenella sp. nov. isolated from soil."; RL Submitted (MAR-2018) to the EMBL/GenBank/DDBJ databases. CC -!- SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase CC superfamily. RNA M5U methyltransferase family. {ECO:0000256|PROSITE- CC ProRule:PRU01024, ECO:0000256|SAAS:SAAS00561307}. CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ CC whole genome shotgun (WGS) entry which is preliminary data. CC {ECO:0000313|EMBL:PSS67205.1}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; PVZR01000001; PSS67205.1; -; Genomic_DNA. DR RefSeq; WP_025426144.1; NZ_PVZR01000001.1. DR Proteomes; UP000240263; Unassembled WGS sequence. DR GO; GO:0008173; F:RNA methyltransferase activity; IEA:InterPro. DR GO; GO:0006396; P:RNA processing; IEA:InterPro. DR InterPro; IPR030390; MeTrfase_TrmA_AS. DR InterPro; IPR029063; SAM-dependent_MTases. DR InterPro; IPR010280; U5_MeTrfase_fam. DR PANTHER; PTHR11061; PTHR11061; 1. DR Pfam; PF05958; tRNA_U5-meth_tr; 1. DR SUPFAM; SSF53335; SSF53335; 1. DR PROSITE; PS51687; SAM_MT_RNA_M5U; 1. DR PROSITE; PS01230; TRMA_1; 1. PE 3: Inferred from homology; KW Methyltransferase {ECO:0000256|PROSITE-ProRule:PRU01024, KW ECO:0000256|SAAS:SAAS00234999, ECO:0000313|EMBL:PSS67205.1}; KW S-adenosyl-L-methionine {ECO:0000256|PROSITE-ProRule:PRU01024, KW ECO:0000256|SAAS:SAAS00235009}; KW Transferase {ECO:0000256|PROSITE-ProRule:PRU01024, KW ECO:0000256|SAAS:SAAS00506843, ECO:0000313|EMBL:PSS67205.1}. FT ACT_SITE 374 FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10015" FT ACT_SITE 374 FT /note="Nucleophile" FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01024" FT BINDING 253 FT /note="S-adenosyl-L-methionine" FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01024" FT BINDING 280 FT /note="S-adenosyl-L-methionine; via carbonyl oxygen" FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01024" FT BINDING 300 FT /note="S-adenosyl-L-methionine" FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01024" FT BINDING 348 FT /note="S-adenosyl-L-methionine" FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01024" SQ SEQUENCE 416 AA; 44264 MW; 72CEC1CDA0E54974 CRC64; MSTQTLTIAR LGAQGDGIAE TEAGPVYAPF TLPGESAALA VNKSQGTLIS LREASPDRVE PHCRHFGPDG QNGTCGGCTL QHAADGLYQT FKRDLVINAL KSKGLKPDVA PLITAQPGER RRAVFTARKT EKDLLLGFNQ AESHHIVAIS ECPVTSPGIV SRLATIGKIG AAMAVNAEPF RITVLETLSG LDLSVDGIKG FGDRERRRTV ETVLGERGIA RVSLNGEIIV EPTKPVIEFG GVSVSPPPGG FTQATLAAEE AMAALVLDHI GKAKRVADLF AGSGTFALRI ARKARVHAVE GEDKALKALD FSARNTQGLK PVTIEKRDLF RRPLMAQELK VYDAIVFDPP RAGADVQCQE IARSGVKKIV AVSCNPTTLA RDLAILVAAG YRITSVTPID QFLWSAHVEA VATLEK //