ID A0A2T3DEL7_9RHIZ Unreviewed; 416 AA. AC A0A2T3DEL7; DT 18-JUL-2018, integrated into UniProtKB/TrEMBL. DT 18-JUL-2018, sequence version 1. DT 12-SEP-2018, entry version 2. DE SubName: Full=Class I SAM-dependent RNA methyltransferase {ECO:0000313|EMBL:PSS67205.1}; GN ORFNames=C6558_04125 {ECO:0000313|EMBL:PSS67205.1}; OS Ensifer sp. NM-2. OC Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales; OC Rhizobiaceae; Sinorhizobium/Ensifer group; Ensifer. OX NCBI_TaxID=2109730 {ECO:0000313|EMBL:PSS67205.1, ECO:0000313|Proteomes:UP000240263}; RN [1] {ECO:0000313|EMBL:PSS67205.1, ECO:0000313|Proteomes:UP000240263} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=NM-2 {ECO:0000313|EMBL:PSS67205.1, RC ECO:0000313|Proteomes:UP000240263}; RA Lund M.K., Andersen N., Dalskov L., Marshall I.P.G., Finster K., RA Schramm A.; RT "Genome sequence of Ensifer ninsenella sp. nov. isolated from soil."; RL Submitted (MAR-2018) to the EMBL/GenBank/DDBJ databases. CC -!- SIMILARITY: Belongs to the class I-like SAM-binding CC methyltransferase superfamily. RNA M5U methyltransferase family. CC {ECO:0000256|PROSITE-ProRule:PRU01024, CC ECO:0000256|SAAS:SAAS00828973}. CC -!- CAUTION: The sequence shown here is derived from an CC EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is CC preliminary data. {ECO:0000313|EMBL:PSS67205.1}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; PVZR01000001; PSS67205.1; -; Genomic_DNA. DR RefSeq; WP_025426144.1; NZ_PVZR01000001.1. DR Proteomes; UP000240263; Unassembled WGS sequence. DR GO; GO:0008168; F:methyltransferase activity; IEA:UniProtKB-KW. DR InterPro; IPR030390; MeTrfase_TrmA_AS. DR InterPro; IPR029063; SAM-dependent_MTases. DR InterPro; IPR010280; U5_MeTrfase_fam. DR PANTHER; PTHR11061; PTHR11061; 1. DR Pfam; PF05958; tRNA_U5-meth_tr; 1. DR SUPFAM; SSF53335; SSF53335; 1. DR PROSITE; PS51687; SAM_MT_RNA_M5U; 1. DR PROSITE; PS01230; TRMA_1; 1. PE 3: Inferred from homology; KW Complete proteome {ECO:0000313|Proteomes:UP000240263}; KW Methyltransferase {ECO:0000256|PROSITE-ProRule:PRU01024, KW ECO:0000256|SAAS:SAAS00828993, ECO:0000313|EMBL:PSS67205.1}; KW S-adenosyl-L-methionine {ECO:0000256|PROSITE-ProRule:PRU01024, KW ECO:0000256|SAAS:SAAS00829012}; KW Transferase {ECO:0000256|PROSITE-ProRule:PRU01024, KW ECO:0000256|SAAS:SAAS00828989, ECO:0000313|EMBL:PSS67205.1}. FT ACT_SITE 374 374 {ECO:0000256|PROSITE-ProRule:PRU10015}. FT ACT_SITE 374 374 Nucleophile. {ECO:0000256|PROSITE- FT ProRule:PRU01024}. FT BINDING 253 253 S-adenosyl-L-methionine. FT {ECO:0000256|PROSITE-ProRule:PRU01024}. FT BINDING 280 280 S-adenosyl-L-methionine; via carbonyl FT oxygen. {ECO:0000256|PROSITE-ProRule: FT PRU01024}. FT BINDING 300 300 S-adenosyl-L-methionine. FT {ECO:0000256|PROSITE-ProRule:PRU01024}. FT BINDING 348 348 S-adenosyl-L-methionine. FT {ECO:0000256|PROSITE-ProRule:PRU01024}. SQ SEQUENCE 416 AA; 44264 MW; 72CEC1CDA0E54974 CRC64; MSTQTLTIAR LGAQGDGIAE TEAGPVYAPF TLPGESAALA VNKSQGTLIS LREASPDRVE PHCRHFGPDG QNGTCGGCTL QHAADGLYQT FKRDLVINAL KSKGLKPDVA PLITAQPGER RRAVFTARKT EKDLLLGFNQ AESHHIVAIS ECPVTSPGIV SRLATIGKIG AAMAVNAEPF RITVLETLSG LDLSVDGIKG FGDRERRRTV ETVLGERGIA RVSLNGEIIV EPTKPVIEFG GVSVSPPPGG FTQATLAAEE AMAALVLDHI GKAKRVADLF AGSGTFALRI ARKARVHAVE GEDKALKALD FSARNTQGLK PVTIEKRDLF RRPLMAQELK VYDAIVFDPP RAGADVQCQE IARSGVKKIV AVSCNPTTLA RDLAILVAAG YRITSVTPID QFLWSAHVEA VATLEK //