ID A0A2R6QA08_ACTCC Unreviewed; 100 AA. AC A0A2R6QA08; DT 20-JUN-2018, integrated into UniProtKB/TrEMBL. DT 20-JUN-2018, sequence version 1. DT 28-JUN-2023, entry version 13. DE RecName: Full=U-box domain-containing protein {ECO:0000256|RuleBase:RU369093, ECO:0000259|PROSITE:PS51698}; DE EC=2.3.2.27 {ECO:0000256|RuleBase:RU369093}; DE AltName: Full=RING-type E3 ubiquitin transferase PUB {ECO:0000256|RuleBase:RU369093}; GN ORFNames=CEY00_Acc20594 {ECO:0000313|EMBL:PSS04736.1}; OS Actinidia chinensis var. chinensis (Chinese soft-hair kiwi). OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC asterids; Ericales; Actinidiaceae; Actinidia. OX NCBI_TaxID=1590841 {ECO:0000313|EMBL:PSS04736.1, ECO:0000313|Proteomes:UP000241394}; RN [1] {ECO:0000313|EMBL:PSS04736.1, ECO:0000313|Proteomes:UP000241394} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=cv. Red5 {ECO:0000313|Proteomes:UP000241394}; RC TISSUE=Young leaf {ECO:0000313|EMBL:PSS04736.1}; RA Pilkington S., Crowhurst R., Hilario E., Nardozza S., Fraser L., Peng Y., RA Gunaseelan K., Simpson R., Tahir J., Deroles S., Templeton K., Luo Z., RA Davy M., Cheng C., Mcneilage M., Scaglione D., Liu Y., Zhang Q., Datson P., RA De Silva N., Gardiner S., Bassett H., Chagne D., Mccallum J., Dzierzon H., RA Deng C., Wang Y.-Y., Barron N., Manako K., Bowen J., Foster T., Erridge Z., RA Tiffin H., Waite C., Davies K., Grierson E., Laing W., Kirk R., Chen X., RA Wood M., Montefiori M., Brummell D., Schwinn K., Catanach A., Fullerton C., RA Li D., Meiyalaghan S., Nieuwenhuizen N., Read N., Prakash R., Hunter D., RA Zhang H., Mckenzie M., Knabel M., Harris A., Allan A., Chen A., Janssen B., RA Plunkett B., Dwamena C., Voogd C., Leif D., Lafferty D., Souleyre E., RA Varkonyi-Gasic E., Gambi F., Hanley J., Yao J.-L., Cheung J., David K., RA Warren B., Marsh K., Snowden K., Lin-Wang K., Brian L., RA Martinez-Sanchez M., Wang M., Ileperuma N., Macnee N., Campin R., RA Mcatee P., Drummond R., Espley R., Ireland H., Wu R., Atkinson R., RA Karunairetnam S., Bulley S., Chunkath S., Hanley Z., Storey R., RA Thrimawithana A., Thomson S., David C., Testolin R.; RT "An improved, manually edited Actinidia chinensis var. chinensis RT (kiwifruit) genome highlights the challenges associated with draft genomes RT and gene prediction in plants."; RL Submitted (JUL-2017) to the EMBL/GenBank/DDBJ databases. RN [2] {ECO:0000313|Proteomes:UP000241394} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=cv. Red5 {ECO:0000313|Proteomes:UP000241394}; RX PubMed=29661190; DOI=10.1186/s12864-018-4656-3; RA Pilkington S.M., Crowhurst R., Hilario E., Nardozza S., Fraser L., Peng Y., RA Gunaseelan K., Simpson R., Tahir J., Deroles S.C., Templeton K., Luo Z., RA Davy M., Cheng C., McNeilage M., Scaglione D., Liu Y., Zhang Q., Datson P., RA De Silva N., Gardiner S.E., Bassett H., Chagne D., McCallum J., RA Dzierzon H., Deng C., Wang Y.Y., Barron L., Manako K., Bowen J., RA Foster T.M., Erridge Z.A., Tiffin H., Waite C.N., Davies K.M., RA Grierson E.P., Laing W.A., Kirk R., Chen X., Wood M., Montefiori M., RA Brummell D.A., Schwinn K.E., Catanach A., Fullerton C., Li D., RA Meiyalaghan S., Nieuwenhuizen N., Read N., Prakash R., Hunter D., Zhang H., RA McKenzie M., Knabel M., Harris A., Allan A.C., Gleave A., Chen A., RA Janssen B.J., Plunkett B., Ampomah-Dwamena C., Voogd C., Leif D., RA Lafferty D., Souleyre E.J.F., Varkonyi-Gasic E., Gambi F., Hanley J., RA Yao J.L., Cheung J., David K.M., Warren B., Marsh K., Snowden K.C., RA Lin-Wang K., Brian L., Martinez-Sanchez M., Wang M., Ileperuma N., RA Macnee N., Campin R., McAtee P., Drummond R.S.M., Espley R.V., RA Ireland H.S., Wu R., Atkinson R.G., Karunairetnam S., Bulley S., RA Chunkath S., Hanley Z., Storey R., Thrimawithana A.H., Thomson S., RA David C., Testolin R., Huang H., Hellens R.P., Schaffer R.J.; RT "A manually annotated Actinidia chinensis var. chinensis (kiwifruit) genome RT highlights the challenges associated with draft genomes and gene prediction RT in plants."; RL BMC Genomics 19:257-257(2018). CC -!- FUNCTION: Functions as an E3 ubiquitin ligase. CC {ECO:0000256|RuleBase:RU369093}. CC -!- CATALYTIC ACTIVITY: CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L- CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.; CC EC=2.3.2.27; Evidence={ECO:0000256|RuleBase:RU369093}; CC -!- PATHWAY: Protein modification; protein ubiquitination. CC {ECO:0000256|ARBA:ARBA00004906, ECO:0000256|RuleBase:RU369093}. CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ CC whole genome shotgun (WGS) entry which is preliminary data. CC {ECO:0000313|EMBL:PSS04736.1}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; NKQK01000018; PSS04736.1; -; Genomic_DNA. DR AlphaFoldDB; A0A2R6QA08; -. DR EnsemblPlants; PSS04736; PSS04736; CEY00_Acc20594. DR Gramene; PSS04736; PSS04736; CEY00_Acc20594. DR InParanoid; A0A2R6QA08; -. DR UniPathway; UPA00143; -. DR Proteomes; UP000241394; Chromosome lg18. DR GO; GO:0061630; F:ubiquitin protein ligase activity; IEA:UniProtKB-UniRule. DR GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniRule. DR CDD; cd16664; RING-Ubox_PUB; 1. DR Gene3D; 3.30.40.10; Zinc/RING finger domain, C3HC4 (zinc finger); 1. DR InterPro; IPR045185; PUB22/23/24-like. DR InterPro; IPR045210; RING-Ubox_PUB. DR InterPro; IPR003613; Ubox_domain. DR InterPro; IPR013083; Znf_RING/FYVE/PHD. DR PANTHER; PTHR22849:SF112; U-BOX DOMAIN-CONTAINING PROTEIN 26; 1. DR PANTHER; PTHR22849; WDSAM1 PROTEIN; 1. DR Pfam; PF04564; U-box; 1. DR SMART; SM00504; Ubox; 1. DR SUPFAM; SSF57850; RING/U-box; 1. DR PROSITE; PS51698; U_BOX; 1. PE 4: Predicted; KW Reference proteome {ECO:0000313|Proteomes:UP000241394}; KW Transferase {ECO:0000256|RuleBase:RU369093}; KW Ubl conjugation pathway {ECO:0000256|RuleBase:RU369093}. FT DOMAIN 4..55 FT /note="U-box" FT /evidence="ECO:0000259|PROSITE:PS51698" SQ SEQUENCE 100 AA; 11338 MW; 742E1AAD0648E935 CRC64; MTNDFPNDFR CPISLEIMFD PVILSSGHTF DRPSIQRWLD TGHWACPITK LPLSEPPSLI PNHAPPEPNF QLYPRLSPQF TARPGPTNPN LHSHLPIFTV //