ID A0A2P1IT77_MERS Unreviewed; 7078 AA. AC A0A2P1IT77; DT 23-MAY-2018, integrated into UniProtKB/TrEMBL. DT 23-MAY-2018, sequence version 1. DT 03-AUG-2022, entry version 26. DE RecName: Full=Replicase polyprotein 1ab {ECO:0000256|ARBA:ARBA00022087}; DE AltName: Full=ORF1ab polyprotein {ECO:0000256|ARBA:ARBA00029611}; GN Name=orf1ab {ECO:0000313|EMBL:AVN89332.1}; OS Middle East respiratory syndrome-related coronavirus (MERS-CoV). OC Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; OC Nidovirales; Cornidovirineae; Coronaviridae; Orthocoronavirinae; OC Betacoronavirus; Merbecovirus. OX NCBI_TaxID=1335626 {ECO:0000313|EMBL:AVN89332.1}; RN [1] {ECO:0000313|EMBL:AVN89332.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=MERS-CoV camel/Burkina Faso/CIRAD-HKU434/2015 RC {ECO:0000313|EMBL:AVN89332.1}; RX PubMed=29507189; RA Chu D.K.W., Hui K.P.Y., Perera R.A.P.M., Miguel E., Niemeyer D., Zhao J., RA Channappanavar R., Dudas G., Oladipo J.O., Traore A., Fassi-Fihri O., RA Ali A., Demissie G.F., Muth D., Chan M.C.W., Nicholls J.M., Meyerholz D.K., RA Kuranga S.A., Mamo G., Zhou Z., So R.T.Y., Hemida M.G., Webby R.J., RA Roger F., Rambaut A., Poon L.L.M., Perlman S., Drosten C., Chevalier V., RA Peiris M.; RT "MERS coronaviruses from camels in Africa exhibit region-dependent genetic RT diversity."; RL Proc. Natl. Acad. Sci. U.S.A. 115:3144-3149(2018). CC -!- FUNCTION: Cleaves the C-terminus of replicase polyprotein at 11 sites. CC Recognizes substrates containing the core sequence [ILMVF]-Q-|-[SGACN]. CC Also able to bind an ADP-ribose-1''-phosphate (ADRP). CC {ECO:0000256|ARBA:ARBA00002223}. CC -!- FUNCTION: Enzyme possessing two different activities: an CC exoribonuclease activity acting on both ssRNA and dsRNA in a 3' to 5' CC direction and a N7-guanine methyltransferase activity. CC {ECO:0000256|ARBA:ARBA00003995}. CC -!- FUNCTION: Forms a hexadecamer with nsp7 (8 subunits of each) that may CC participate in viral replication by acting as a primase. Alternatively, CC may synthesize substantially longer products than oligonucleotide CC primers. {ECO:0000256|ARBA:ARBA00002182}. CC -!- FUNCTION: Forms a hexadecamer with nsp8 (8 subunits of each) that may CC participate in viral replication by acting as a primase. Alternatively, CC may synthesize substantially longer products than oligonucleotide CC primers. {ECO:0000256|ARBA:ARBA00003443}. CC -!- FUNCTION: May participate in viral replication by acting as a ssRNA- CC binding protein. {ECO:0000256|ARBA:ARBA00002012}. CC -!- FUNCTION: May play a role in the modulation of host cell survival CC signaling pathway by interacting with host PHB and PHB2. Indeed, these CC two proteins play a role in maintaining the functional integrity of the CC mitochondria and protecting cells from various stresses. CC {ECO:0000256|ARBA:ARBA00003115}. CC -!- FUNCTION: Methyltransferase that mediates mRNA cap 2'-O-ribose CC methylation to the 5'-cap structure of viral mRNAs. N7-methyl guanosine CC cap is a prerequisite for binding of nsp16. Therefore plays an CC essential role in viral mRNAs cap methylation which is essential to CC evade immune system. {ECO:0000256|ARBA:ARBA00002840}. CC -!- FUNCTION: Multi-functional protein with a zinc-binding domain in N- CC terminus displaying RNA and DNA duplex-unwinding activities with 5' to CC 3' polarity. Activity of helicase is dependent on magnesium. CC {ECO:0000256|ARBA:ARBA00002960}. CC -!- FUNCTION: Participates in the assembly of virally-induced cytoplasmic CC double-membrane vesicles necessary for viral replication. CC {ECO:0000256|ARBA:ARBA00003070}. CC -!- FUNCTION: Plays a pivotal role in viral transcription by stimulating CC both nsp14 3'-5' exoribonuclease and nsp16 2'-O-methyltransferase CC activities. Therefore plays an essential role in viral mRNAs cap CC methylation. {ECO:0000256|ARBA:ARBA00002697}. CC -!- FUNCTION: Plays a role in the initial induction of autophagosomes from CC host reticulum endoplasmic. Later, limits the expansion of these CC phagosomes that are no longer able to deliver viral components to CC lysosomes. {ECO:0000256|ARBA:ARBA00003748}. CC -!- FUNCTION: Plays a role in viral transcription/replication and prevents CC the simultaneous activation of host cell dsRNA sensors, such as CC MDA5/IFIH1, OAS, and PKR (By similarity). Acts by degrading the 5'- CC polyuridines generated during replication of the poly(A) region of CC viral genomic and subgenomic RNAs. Catalyzes a two-step reaction in CC which a 2'3'-cyclic phosphate (2'3'-cP) is first generated by 2'-O CC transesterification, which is then hydrolyzed to a 3'-phosphate (3'-P) CC (By similarity). If not degraded, poly(U) RNA would hybridize with CC poly(A) RNA tails and activate host dsRNA sensors. CC {ECO:0000256|ARBA:ARBA00025521}. CC -!- FUNCTION: Responsible for replication and transcription of the viral CC RNA genome. {ECO:0000256|ARBA:ARBA00003927}. CC -!- FUNCTION: The replicase polyprotein of coronaviruses is a CC multifunctional protein: it contains the activities necessary for the CC transcription of negative stranded RNA, leader RNA, subgenomic mRNAs CC and progeny virion RNA as well as proteinases responsible for the CC cleavage of the polyprotein into functional products. CC {ECO:0000256|ARBA:ARBA00003368}. CC -!- CATALYTIC ACTIVITY: CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065, CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12; CC Evidence={ECO:0000256|ARBA:ARBA00001665}; CC -!- CATALYTIC ACTIVITY: CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065, CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13; CC Evidence={ECO:0000256|ARBA:ARBA00001556}; CC -!- CATALYTIC ACTIVITY: CC Reaction=Thiol-dependent hydrolysis of ester, thioester, amide, peptide CC and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76- CC residue protein attached to proteins as an intracellular targeting CC signal).; EC=3.4.19.12; Evidence={ECO:0000256|ARBA:ARBA00000707}; CC -!- CATALYTIC ACTIVITY: CC Reaction=a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-ribonucleoside CC in mRNA + S-adenosyl-L-methionine = a 5'-end (N(7)-methyl 5'- CC triphosphoguanosine)-(2'-O-methyl-ribonucleoside) in mRNA + H(+) + S- CC adenosyl-L-homocysteine; Xref=Rhea:RHEA:67020, Rhea:RHEA-COMP:17167, CC Rhea:RHEA-COMP:17168, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, CC ChEBI:CHEBI:59789, ChEBI:CHEBI:156461, ChEBI:CHEBI:167609; CC EC=2.1.1.57; Evidence={ECO:0000256|ARBA:ARBA00024256}; CC -!- CATALYTIC ACTIVITY: CC Reaction=uridylyl-uridylyl-ribonucleotide-RNA = a 3'-end uridylyl- CC 2',3'-cyclophospho-uridine-RNA + a 5'-end dephospho-ribonucleoside- CC RNA; Xref=Rhea:RHEA:67732, Rhea:RHEA-COMP:13936, Rhea:RHEA- CC COMP:17334, Rhea:RHEA-COMP:17335, ChEBI:CHEBI:138284, CC ChEBI:CHEBI:173079, ChEBI:CHEBI:173080; CC Evidence={ECO:0000256|ARBA:ARBA00024600}; CC -!- COFACTOR: CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035; CC Evidence={ECO:0000256|ARBA:ARBA00001936}; CC -!- SUBUNIT: Nsp2 interacts with host PHB and PHB2. 3CL-PRO exists as CC monomer and homodimer. Nsp4 interacts with PL-PRO and nsp6. Only the CC homodimer shows catalytic activity. Eight copies of nsp7 and eight CC copies of nsp8 assemble to form a heterohexadecamer dsRNA-encircling CC ring structure. Nsp9 is a dimer. Nsp10 forms a dodecamer and interacts CC with nsp14 and nsp16; these interactions enhance nsp14 and nsp16 CC enzymatic activities. Nsp14 interacts (via N-terminus) with DDX1. CC {ECO:0000256|ARBA:ARBA00011652}. CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}. CC Endoplasmic reticulum-Golgi intermediate compartment CC {ECO:0000256|ARBA:ARBA00004399}. Host cytoplasm, host perinuclear CC region {ECO:0000256|ARBA:ARBA00004407}. Host endoplasmic reticulum- CC Golgi intermediate compartment {ECO:0000256|ARBA:ARBA00004452}. Host CC membrane {ECO:0000256|ARBA:ARBA00004301}; Multi-pass membrane protein CC {ECO:0000256|ARBA:ARBA00004301}. Membrane CC {ECO:0000256|ARBA:ARBA00004141}; Multi-pass membrane protein CC {ECO:0000256|ARBA:ARBA00004141}. CC -!- SIMILARITY: Belongs to the coronaviruses polyprotein 1ab family. CC {ECO:0000256|ARBA:ARBA00008087}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; MG923470; AVN89332.1; -; Genomic_RNA. DR GO; GO:0005793; C:endoplasmic reticulum-Golgi intermediate compartment; IEA:UniProtKB-SubCell. DR GO; GO:0044172; C:host cell endoplasmic reticulum-Golgi intermediate compartment; IEA:UniProtKB-SubCell. DR GO; GO:0033644; C:host cell membrane; IEA:UniProtKB-SubCell. DR GO; GO:0044220; C:host cell perinuclear region of cytoplasm; IEA:UniProtKB-SubCell. DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW. DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW. DR GO; GO:0004843; F:cysteine-type deubiquitinase activity; IEA:UniProtKB-EC. DR GO; GO:0004197; F:cysteine-type endopeptidase activity; IEA:InterPro. DR GO; GO:0003678; F:DNA helicase activity; IEA:UniProtKB-EC. DR GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW. DR GO; GO:0004532; F:exoribonuclease activity; IEA:InterPro. DR GO; GO:0002151; F:G-quadruplex RNA binding; IEA:InterPro. DR GO; GO:0042802; F:identical protein binding; IEA:UniProt. DR GO; GO:0016829; F:lyase activity; IEA:UniProtKB-KW. DR GO; GO:0008168; F:methyltransferase activity; IEA:UniProtKB-KW. DR GO; GO:0008242; F:omega peptidase activity; IEA:InterPro. DR GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC. DR GO; GO:0003968; F:RNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-KW. DR GO; GO:0003727; F:single-stranded RNA binding; IEA:InterPro. DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro. DR GO; GO:0039595; P:induction by virus of catabolism of host mRNA; IEA:UniProtKB-KW. DR GO; GO:0039520; P:induction by virus of host autophagy; IEA:UniProtKB-KW. DR GO; GO:0032259; P:methylation; IEA:UniProtKB-KW. DR GO; GO:0039648; P:modulation by virus of host protein ubiquitination; IEA:UniProtKB-KW. DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW. DR GO; GO:0039657; P:suppression by virus of host gene expression; IEA:UniProtKB-KW. DR GO; GO:0039579; P:suppression by virus of host ISG15-protein conjugation; IEA:UniProtKB-KW. DR GO; GO:0039644; P:suppression by virus of host NF-kappaB cascade; IEA:UniProtKB-KW. DR GO; GO:0039502; P:suppression by virus of host type I interferon-mediated signaling pathway; IEA:UniProtKB-KW. DR GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro. DR GO; GO:0019082; P:viral protein processing; IEA:InterPro. DR GO; GO:0039694; P:viral RNA genome replication; IEA:InterPro. DR CDD; cd21409; 1B_cv_Nsp13-like; 1. DR CDD; cd21560; betaCoV-Nsp6; 1. DR CDD; cd21659; betaCoV_Nsp14; 1. DR CDD; cd21666; betaCoV_Nsp5_Mpro; 1. DR CDD; cd20762; capping_2-OMTase_Nidovirales; 1. DR CDD; cd21517; cv_beta_Nsp2_MERS-like; 1. DR CDD; cd21473; cv_Nsp4_TM; 1. DR CDD; cd21165; M_cv-Nsp15-like; 1. DR CDD; cd21563; Macro_cv_SUD-M_Nsp3-like; 1. DR CDD; cd21557; Macro_X_Nsp3-like; 1. DR CDD; cd21592; MERS-CoV-like_RdRp; 1. DR CDD; cd21161; NendoU_cv_Nsp15-like; 1. DR CDD; cd21171; NTD_alpha_beta_cv_Nsp15-like; 1. DR CDD; cd21523; SUD_C_MERS-CoV_Nsp3; 1. DR CDD; cd21467; Ubl1_cv_Nsp3_N-like; 1. DR CDD; cd21466; Ubl2_cv_PLpro_N_Nsp3-like; 1. DR CDD; cd21401; ZBD_cv_Nsp13-like; 1. DR Gene3D; 1.10.150.420; -; 1. DR Gene3D; 1.10.1840.10; -; 1. DR Gene3D; 1.10.8.1190; -; 1. DR Gene3D; 1.10.8.370; -; 1. DR Gene3D; 2.40.10.10; -; 2. DR Gene3D; 2.40.10.250; -; 1. DR Gene3D; 2.60.120.1680; -; 1. DR Gene3D; 3.10.20.350; -; 1. DR Gene3D; 3.10.20.540; -; 1. DR Gene3D; 3.30.160.820; -; 1. DR Gene3D; 3.30.70.3540; -; 1. DR Gene3D; 3.40.220.10; -; 1. DR Gene3D; 3.40.220.20; -; 1. DR Gene3D; 3.40.50.11020; -; 1. DR Gene3D; 3.40.50.11580; -; 1. DR Gene3D; 3.40.50.150; -; 1. DR Gene3D; 3.40.50.300; -; 2. DR InterPro; IPR027351; (+)RNA_virus_helicase_core_dom. DR InterPro; IPR043608; CoV_NSP15_M. DR InterPro; IPR043606; CoV_NSP15_N. DR InterPro; IPR043613; CoV_NSP2_C. DR InterPro; IPR043611; CoV_NSP3_C. DR InterPro; IPR032505; CoV_NSP4_C. DR InterPro; IPR043612; CoV_NSP4_N. DR InterPro; IPR043502; DNA/RNA_pol_sf. DR InterPro; IPR041679; DNA2/NAM7-like_C. DR InterPro; IPR022733; DPUP_SUD_C_bCoV. DR InterPro; IPR037227; EndoU-like. DR InterPro; IPR002589; Macro_dom. DR InterPro; IPR043472; Macro_dom-like. DR InterPro; IPR044371; Macro_X_NSP3-like. DR InterPro; IPR043609; NendoU_nidovirus. DR InterPro; IPR044863; NIRAN. DR InterPro; IPR036333; NSP10_sf_CoV. DR InterPro; IPR044343; NSP13_1B_dom_CoV. DR InterPro; IPR027352; NSP13_ZBD_CoV-like. DR InterPro; IPR044315; NSP14_betaCoV. DR InterPro; IPR009466; NSP14_CoV. DR InterPro; IPR044330; NSP15_alpha_betaCoV_N. DR InterPro; IPR044327; NSP15_M_MERS. DR InterPro; IPR043174; NSP15_middle_sf. DR InterPro; IPR042515; NSP15_N_CoV. DR InterPro; IPR044401; NSP15_NendoU_CoV. DR InterPro; IPR009461; NSP16_CoV-like. DR InterPro; IPR021590; NSP1_bCoV. DR InterPro; IPR044388; NSP2_MERS-like. DR InterPro; IPR043615; NSP2_N_CoV. DR InterPro; IPR024375; NSP3_bCoV. DR InterPro; IPR032592; NSP3_NAB_bCoV. DR InterPro; IPR042570; NSP3_NAB_bCoV_sf. DR InterPro; IPR038400; NSP3_SUD-M_sf_bCoV. DR InterPro; IPR044382; NSP3_SUD_C_MERS-CoV. DR InterPro; IPR044357; NSP3_Ubl1_dom_CoV. DR InterPro; IPR044353; Nsp3_Ubl2_dom_CoV. DR InterPro; IPR038083; NSP3A-like. DR InterPro; IPR038123; NSP4_C_sf_CoV. DR InterPro; IPR044367; NSP6_betaCoV. DR InterPro; IPR043610; NSP6_CoV. DR InterPro; IPR014828; NSP7_CoV. DR InterPro; IPR037204; NSP7_sf_CoV. DR InterPro; IPR014829; NSP8_CoV. DR InterPro; IPR037230; NSP8_sf_CoV. DR InterPro; IPR014822; NSP9_CoV. DR InterPro; IPR036499; NSP9_sf_CoV. DR InterPro; IPR027417; P-loop_NTPase. DR InterPro; IPR013016; Peptidase_C16_CoV. DR InterPro; IPR008740; Peptidase_C30_CoV. DR InterPro; IPR043477; Peptidase_C30_dom3_CoV. DR InterPro; IPR009003; Peptidase_S1_PA. DR InterPro; IPR043504; Peptidase_S1_PA_chymotrypsin. DR InterPro; IPR043177; PLpro_N_sf_CoV. DR InterPro; IPR043503; PLpro_palm_finger_dom_CoV. DR InterPro; IPR043178; PLpro_thumb_sf_CoV. DR InterPro; IPR044350; RdRp_MERS-CoV-like. DR InterPro; IPR001205; RNA-dir_pol_C. DR InterPro; IPR007094; RNA-dir_pol_PSvirus. DR InterPro; IPR009469; RNA_pol_N_CoV. DR InterPro; IPR018995; RNA_synth_NSP10_CoV. DR InterPro; IPR029063; SAM-dependent_MTases_sf. DR Pfam; PF13087; AAA_12; 1. DR Pfam; PF16251; bCoV_NAR; 1. DR Pfam; PF11501; bCoV_NSP1; 1. DR Pfam; PF11633; bCoV_SUD_M; 1. DR Pfam; PF06471; CoV_ExoN; 1. DR Pfam; PF06460; CoV_Methyltr_2; 1. DR Pfam; PF09401; CoV_NSP10; 1. DR Pfam; PF19215; CoV_NSP15_C; 1. DR Pfam; PF19216; CoV_NSP15_M; 1. DR Pfam; PF19219; CoV_NSP15_N; 1. DR Pfam; PF19212; CoV_NSP2_C; 1. DR Pfam; PF19211; CoV_NSP2_N; 1. DR Pfam; PF19218; CoV_NSP3_C; 1. DR Pfam; PF16348; CoV_NSP4_C; 1. DR Pfam; PF19217; CoV_NSP4_N; 1. DR Pfam; PF19213; CoV_NSP6; 1. DR Pfam; PF08716; CoV_NSP7; 1. DR Pfam; PF08717; CoV_NSP8; 1. DR Pfam; PF08710; CoV_NSP9; 1. DR Pfam; PF08715; CoV_peptidase; 1. DR Pfam; PF06478; CoV_RPol_N; 1. DR Pfam; PF01661; Macro; 1. DR Pfam; PF05409; Peptidase_C30; 1. DR Pfam; PF00680; RdRP_1; 1. DR SMART; SM00506; A1pp; 1. DR SUPFAM; SSF101816; SSF101816; 1. DR SUPFAM; SSF140367; SSF140367; 1. DR SUPFAM; SSF142877; SSF142877; 1. DR SUPFAM; SSF143076; SSF143076; 1. DR SUPFAM; SSF144246; SSF144246; 1. DR SUPFAM; SSF159936; SSF159936; 1. DR SUPFAM; SSF50494; SSF50494; 1. DR SUPFAM; SSF52540; SSF52540; 1. DR SUPFAM; SSF52949; SSF52949; 1. DR SUPFAM; SSF53335; SSF53335; 1. DR SUPFAM; SSF56672; SSF56672; 1. DR PROSITE; PS51942; BCOV_NSP3C_C; 1. DR PROSITE; PS51941; BCOV_NSP3C_M; 1. DR PROSITE; PS51945; BCOV_NSP3E_NAB; 1. DR PROSITE; PS51943; COV_NSP3A_UBL; 1. DR PROSITE; PS51944; COV_NSP3D_UBL; 1. DR PROSITE; PS51946; COV_NSP4C; 1. DR PROSITE; PS51653; CV_ZBD; 1. DR PROSITE; PS51442; M_PRO; 1. DR PROSITE; PS51154; MACRO; 1. DR PROSITE; PS51947; NIRAN; 1. DR PROSITE; PS51124; PEPTIDASE_C16; 1. DR PROSITE; PS51657; PSRV_HELICASE; 1. DR PROSITE; PS50507; RDRP_SSRNA_POS; 1. PE 3: Inferred from homology; KW Activation of host autophagy by virus {ECO:0000256|ARBA:ARBA00023050}; KW ATP-binding {ECO:0000256|ARBA:ARBA00022840}; KW Decay of host mRNAs by virus {ECO:0000256|ARBA:ARBA00022616}; KW Endonuclease {ECO:0000256|ARBA:ARBA00022759}; KW Eukaryotic host gene expression shutoff by virus KW {ECO:0000256|ARBA:ARBA00023247}; KW Eukaryotic host translation shutoff by virus KW {ECO:0000256|ARBA:ARBA00022809}; KW Exonuclease {ECO:0000256|ARBA:ARBA00022839}; KW Helicase {ECO:0000256|ARBA:ARBA00022806}; KW Host cytoplasm {ECO:0000256|ARBA:ARBA00023200}; KW Host gene expression shutoff by virus {ECO:0000256|ARBA:ARBA00022995}; KW Host membrane {ECO:0000256|ARBA:ARBA00022870}; KW Host mRNA suppression by virus {ECO:0000256|ARBA:ARBA00022557}; KW Host-virus interaction {ECO:0000256|ARBA:ARBA00022581}; KW Hydrolase {ECO:0000256|ARBA:ARBA00022801}; KW Inhibition of host innate immune response by virus KW {ECO:0000256|ARBA:ARBA00022632}; KW Inhibition of host interferon signaling pathway by virus KW {ECO:0000256|ARBA:ARBA00022830}; KW Inhibition of host ISG15 by virus {ECO:0000256|ARBA:ARBA00023208}; KW Inhibition of host NF-kappa-B by virus {ECO:0000256|ARBA:ARBA00022863}; KW Lyase {ECO:0000256|ARBA:ARBA00023239}; KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius}; KW Metal-binding {ECO:0000256|ARBA:ARBA00022723}; KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603}; KW Modulation of host ubiquitin pathway by viral deubiquitinase KW {ECO:0000256|ARBA:ARBA00022876}; KW Modulation of host ubiquitin pathway by virus KW {ECO:0000256|ARBA:ARBA00022662}; Nuclease {ECO:0000256|ARBA:ARBA00022722}; KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741}; KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695}; KW Protease {ECO:0000256|ARBA:ARBA00022670}; KW Repeat {ECO:0000256|ARBA:ARBA00022737}; KW Ribosomal frameshifting {ECO:0000256|ARBA:ARBA00022758}; KW RNA-binding {ECO:0000256|ARBA:ARBA00022884, ECO:0000256|PROSITE- KW ProRule:PRU01289}; KW RNA-directed RNA polymerase {ECO:0000256|ARBA:ARBA00022484}; KW Thiol protease {ECO:0000256|ARBA:ARBA00022807}; KW Transferase {ECO:0000256|ARBA:ARBA00022679}; KW Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius}; KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989, KW ECO:0000256|SAM:Phobius}; KW Ubl conjugation pathway {ECO:0000256|ARBA:ARBA00022786}; KW Viral immunoevasion {ECO:0000256|ARBA:ARBA00023280}; KW Viral RNA replication {ECO:0000256|ARBA:ARBA00022953}; KW Zinc {ECO:0000256|ARBA:ARBA00022833}; KW Zinc-finger {ECO:0000256|ARBA:ARBA00022771, ECO:0000256|PROSITE- KW ProRule:PRU00986}. FT TRANSMEM 2175..2195 FT /note="Helical" FT /evidence="ECO:0000256|SAM:Phobius" FT TRANSMEM 2307..2325 FT /note="Helical" FT /evidence="ECO:0000256|SAM:Phobius" FT TRANSMEM 2337..2362 FT /note="Helical" FT /evidence="ECO:0000256|SAM:Phobius" FT TRANSMEM 2756..2777 FT /note="Helical" FT /evidence="ECO:0000256|SAM:Phobius" FT TRANSMEM 3024..3049 FT /note="Helical" FT /evidence="ECO:0000256|SAM:Phobius" FT TRANSMEM 3061..3092 FT /note="Helical" FT /evidence="ECO:0000256|SAM:Phobius" FT TRANSMEM 3112..3138 FT /note="Helical" FT /evidence="ECO:0000256|SAM:Phobius" FT TRANSMEM 3564..3582 FT /note="Helical" FT /evidence="ECO:0000256|SAM:Phobius" FT TRANSMEM 3594..3613 FT /note="Helical" FT /evidence="ECO:0000256|SAM:Phobius" FT TRANSMEM 3620..3638 FT /note="Helical" FT /evidence="ECO:0000256|SAM:Phobius" FT TRANSMEM 3669..3687 FT /note="Helical" FT /evidence="ECO:0000256|SAM:Phobius" FT TRANSMEM 3694..3710 FT /note="Helical" FT /evidence="ECO:0000256|SAM:Phobius" FT TRANSMEM 3730..3752 FT /note="Helical" FT /evidence="ECO:0000256|SAM:Phobius" FT TRANSMEM 3764..3786 FT /note="Helical" FT /evidence="ECO:0000256|SAM:Phobius" FT DOMAIN 856..966 FT /note="Ubiquitin-like" FT /evidence="ECO:0000259|PROSITE:PS51943" FT DOMAIN 1110..1276 FT /note="Macro" FT /evidence="ECO:0000259|PROSITE:PS51154" FT DOMAIN 1278..1404 FT /note="Macro" FT /evidence="ECO:0000259|PROSITE:PS51941" FT DOMAIN 1404..1477 FT /note="DPUP" FT /evidence="ECO:0000259|PROSITE:PS51942" FT DOMAIN 1482..1537 FT /note="Ubiquitin-like" FT /evidence="ECO:0000259|PROSITE:PS51944" FT DOMAIN 1552..1823 FT /note="Peptidase C16" FT /evidence="ECO:0000259|PROSITE:PS51124" FT DOMAIN 1837..1954 FT /note="Nucleic acid-binding" FT /evidence="ECO:0000259|PROSITE:PS51945" FT DOMAIN 3151..3247 FT /note="Nsp4C" FT /evidence="ECO:0000259|PROSITE:PS51946" FT DOMAIN 3248..3553 FT /note="Peptidase C30" FT /evidence="ECO:0000259|PROSITE:PS51442" FT DOMAIN 4383..4639 FT /note="NiRAN" FT /evidence="ECO:0000259|PROSITE:PS51947" FT DOMAIN 4990..5152 FT /note="RdRp catalytic" FT /evidence="ECO:0000259|PROSITE:PS50507" FT DOMAIN 5311..5394 FT /note="CV ZBD" FT /evidence="ECO:0000259|PROSITE:PS51653" FT DOMAIN 5567..5923 FT /note="(+)RNA virus helicase C-terminal" FT /evidence="ECO:0000259|PROSITE:PS51657" SQ SEQUENCE 7078 AA; 789909 MW; F3262279F871D4B9 CRC64; MSFVAGVTAQ GARGTYRAAL NSEKHQDHVS LTVPLCGSGN LVEKLSPWFM DGENAYEVVK AMLLKKEPLL YVPIRLAGHT RHLPGPRVYL VERLIACENP FMVNQLAYSS SANGSLVCTT LQGKPIGMFF PYDIELVTGK QNILLRKYGR GGYHYTPFYY ERDNTSCPEW MDDFEADPKG KYAQNLLKKL IGGDVTPVDQ YMCGVDGKPI SAYAFLMAKD GITKLADVEA DVAARADDEG FITLKNNLYR LVWHVERKDV PYPKQSIFTI NSVVQKDGVE NTPPHYFTLG CKILTLTPRN KWSGVSDLSL KQKLLYTFYG KESLENPTYI YHSAFIECGS CGNESWLTGN AIQGFACGCG ASYTANDVEV QSSGMIKPNA LLCATCPFAK GDSCSSNCKH SVAQLVSYLS ERCNVIADSK SFTLIFGGVA YAYFGCEEGT MYFVPRAKSV VSRIGDSIFT GCTGSWNKVT QIANMFLEQT QHSLNFVGEF VVNDVVIAIL SGTTTNVEKI RQLLKGVTLD KLRDYLADYD VAVTAGPFMD DAINVGGTGL QYAAITAPYV VLTGLGESFK KVATIPYKVC YSVKDTLAYY AHSVLYRVFP YDMDSGVSSF SELLFDCVDL SVASTYFLVR LLQDKTGDFM STIITSCQTA VSKFLDTCFE ATEATFNFLL DLAGLFRIFL RNAYVYTSQG FVVVNGKVST LVKQVLDLLN KGMQLLHTKV SWAGSKISAV IYSGRESLIF PSGTYYCVTT KAKSVQQDLD VILPGEFSKK QLGLLQPTDN STTVSVTVSS NMVETVVGQL EQTNMHSPDV IVGDYVIISE KLFVRSKEED GFAFYPACTN GHAVPTLFRL KGGAPVKKVA FGGDQVHEVA AVRSVTVEYN IHAVLDTLLA SSSLRTFVVD KSLSIEEFAD VVKEQVSDLL VKLLRGMPIP DFDLDDFIDA PCYCFNAEGD AFWSSTMIFS LHPVECDEEC SEVEASDLEE GESECISETS TEQVDVSHET SDDEWAAAVD EAFPLDEAEY VTEFVQEEAQ PVEVPVEDIA QVVIADTLQE TPVVPDTVEV PPQVVELPSE PQTIQPEVKE VAPVYEADTE QTQSVTVKPK RLRKKRNVDP LSNFEHKVIT ECVTIVLGDA IQVAKCYGES VLVNAANTHL KHGGGIAGAI NAASKGAVQK ESDEYILAKG PLQVGDSVLL QGHSLAKNIL HVVGPDARAK QDVSLLSKCY KAMNAYPLVV TPLVSAGIFG VKPAVSFDYL IREAKTRVLV VVNSQDVYKS LTIVDIPQSL TFSYDGLRGA IRKAKDYGFT VFVCTDNSAN TKVLRNKGVD YTKKFLTVDG VQYYCYTSKD TLDDILQQAN KSVGIISMPL GYVSHGLDLM QAGSVVRRVN VPYVCLLANK EQEAILMSED VKLNPSEDFI KHVRTNGGYN SWHLVEGELL VQDLRLNKLL HWSDQTICYK DSVFYVVKNS TAFPFETLSA CRAYLDSRTT QQLTIEVLVT VDGVNFRTVV LNNKNTYISQ LGCVFFNGAD ISDTIPDEKQ NGHSLYLADN LTADETKALK ELYGPVDPTF LHRFYSLKAA VHRWKMVVCD KVRFFKLSDN NCYLNAVIMT LDLLKDIKFV IPALQHAFMK HKGGDSTDFI ALIMAYGNCT FGDPDDASRL LHTVLAKAEL CCSARMVWRE WCNVCGIKDV VLQGLKACCY VGVQTVEDLR ARMTYVCQCG GERHQQLVEH TTPWLLLSGT PNEKLVTTST APDFVAFNVF QGIETAVGHY VHARLKGGLI LKFDSGTVSK TSDWKCKVTD VLFPGQKYSS DCNVVRYSLD GNFRTEVDPD LSAFYVKDGK YFTSEPPVTY SPATILAGSV YTNSCLVSSD GQPGGDAISL SFNNLLGFDS SKPVTKKYTY SFLPKEDGDV LLAEFDTYDP TYKNGAMYKG KPILWVNKAS YDTNLNKFNG ASLRQIFDVA PIELENKFTP LSVESTPVEP PTVDVVALQQ EMTIVKCKGL NKPFVKDNVI FVADDSGTPV VEYLSKEDLH TLYVDPKYQV IVLKDNVLSS MLRLHTVESG DINVVAASGS LTRKVKLLFR ASFYFKEFAT RTFTATTAVG SCIKSVVRHL GVTKGILTGC FSFVKMLFML PLAYFSDSKL GTTEVKVSAL KTAGVVTGNV VKQCCTAAVD LSMDKLCRVD WKSTLRLLLM LCTTMVLLSS VYHLYVFNQV LSSDVMFEDA QGLKKFYKEV RAYLGISSAC DGLASAYRAN SFDVPTFCAN RSAMCNWCLI SQDSITHYPA LKMVQTHLSH YVLNIDWLWF AFETGLAYML YTSAFNWLLL AGTLHYFFAQ TSIFVDWRSY NYVVSSAFWL FTHIPMAGLV RMYNLLACLW LLRKFYQHVI NGCKDTACLL CYKRNRLTRV EASTVVCGGK RTFYITANGG ISFCHRHNWN CVDCDAAGVG NTFICEEVAN DLTTALRRPI NATDRSHYYV DSVTVKETVV QFNYRRDGQP CYERFPLCAF TNLDKLKFKE VCKTTTGIPE YNFIIYDSSD RGQESLARSA CVYYSQVLCK SILLVDSSLV TSVGDSSEIA TKMFDSFVNS FVSLYNVTRD KLEKLISTAR DGVRRGDNFH SVLTTFIDAA RGPAGVESDV ETNEIVDSVQ YAHKHDIQLT NESYNNYVPS YVKPDSVSTS DLGSLIDCNA ASVNQTVLRN SNGACIWNAA AYMKLSDALK RQIRIACRKC NLAFRLTTSK LRANDNILSV RFTANKIVGG APTWFNALRD FTLKGYVLAT IIVFLCAVLM YLCLPTFSMA PVEFYEDRIL DFKVLDNGII RDVNPDDKCF ANKHRSFTQW YHEHIGGVYD NSITCPLTVA VIAGVAGARI PDVPTTLAWV NNQIIFFVSR VFANTGSVCY TPIDEIPYKS FSDSGCILPS ECTMFRDAEG RMTPYCHDPT VLPGAFAYSQ MRPHVRYDLY DGNMFIKFPE VVFESTLRIT RTLSTQYCRF GSCEYAQEGV CITTNGSWAI FNDHHLNRPG VYCGSDFIDI VRRLAVSLFQ PITYFQLTTS LVLGIGLCAF LTLLFYYINK VKRAFADYTQ CAVIAVVAAV LNSLCICFVA SIPLCIVPYT ALYYYATFYF TNEPAFIMHV SWYIMFGPIV PIWMTCVYTV AMCFRHFFWV LAYFSKKHVE VFTDGRLNCS FQDAASNIFV INKDTYAALR NSLTNDAYSR FLGLFNKYKY FSGAMETAAY REAAACHLAK ALQTYSETGS DLLYQPPNCS ITSGVLQSGL VKMSHPSGDV EACMVQVTCG SMTLNGLWLD NTVWCPRHVM CPADQLSDPN YDALLISMTN HSFSVQKHIG APANLRVVGH AMQGTLLKLT VDVANPSTPA YTFTTVKPGA SFSVLACYNG RPTGTFTVVM RPNYTIKGSF LCGSCGSVGY TKEGSVINFC YMHQMELANG THTGSAFDGT MYGAFMDKQV HQVQLTDKYC SVNVVAWLYA AILNGCAWFV KPNRTSVVSF NEWALANQFT EFVGTQSVDM LAVKTGVAIE QLLYAIQQLY TGFQGKQILG STMLEDEFTP EDVNMQIMGV VMQSGVRKVT YGTAHWLFAT LVSTYVIILQ ATKFTLWNYL FETIPTQLFP LLFVTMAFVM LLVKHKHTFL TLFLLPVAIC LTYANIVYEP TTPISSALIA VANWLAPTNA YMRTTHTDIG VYISMSLVLV IVVKRLYNPS LSNFALALCS GVMWLYTYSI GEASSPIAYL IFVTTLTSDY TITVFVTVNL AKVCTYAIFA YSPQLTLVFP EVKMILLLYT CLGFMCTCYF GVFSLLNLKL RAPMGVYDFK VSTQEFRFMT ANNLTAPRNS WEAMALNFKL IGIGGTPCIK VAAMQSKLTD LKCISVVLLS VLQQLHLEAN SRAWAFCVKC HNDILAATDP SEAFEKFVSL FATLMTFSGN VDLDALASDI FDTPSVLQAT LSEFSHLATF AELEAAQKAY QEAMDSGDTS PQVLKALQKA VNIAKNAYEK DKAVARKLER MADQAMTSMY KQARAEDKKA KIISAMQTML FGMIKKLDND VLNGIISNAR NGCIPLSVIP LCASNKLRVV IPDFTVWNQV VTYPSLNYAG ALWDITVINN VDNEIVKSSD VVDSNENLTW PLVLECTRAS TSAVKLQNNE IKPSGLKTMV VSAGQEQTNC NTSSLAYYEP VQGRKMLMAL LSDNAYLKWA RVEGKDGFVS VELQPPCKFL IAGPKGPEIR YLYFVKNLNN LHRGQVLGHI AATVRLQAGS NTEFASNSSV LSLVNFTVDP QKAYLDFVNA GGAPLTNCVK MLTPKTGTGI AISVKPESTA DQETYGGASV CLYCRAHIEH PDVSGVCKYK GKFVQIPAQC VRDPVGFCLS NTPCNVCQYW IGYGCNCDSL RQAALPQSKD SNFLNRVRGS IVNARIEPCS SGLSTDVVFR AFDICNYKAK VAGIGKYYKT NTCRFVELDD QGHHLDSYFV VKRHTMENYE LEKHCYDLLR DCDAVAPHDF FIFDVDKVKT PHIVRQRLTE YTMMDLVYAL RHFDQNSEVL KAILVKYGCC DVTYFENKLW FDFVENPSVI GVYHKLGERV RQAILNTVKF CDHIVKAGLV GVLTLDNQDL NGKWYDFGDF VITQPGSGVA IVDSYYSYLM PVLSMTDCLA AETHRDCDFN KPLIEWPLTE YDFTDYKVQL FEKYFKYWDQ TYHANCVNCT DDRCVLHCAN FNVLFAMTMP KTCFGPIVRK IFVDGVPFVV SCGYHYKELG LVMNMDVSVH RHRLSLKELM MYAADPAMHI ASSNAFLDLR TSCFSVAALT TGLTFQTVRP GNFNQDFYDF VVSKGFFKEG SSVTLKHFFF AQDGNAAITD YNYYSYNLPT MCDIKQMLFC MEVVNKYFEI YDGGCLNASE VVVNNLDKSA GHPFNKFGKA RVYYESMSYQ EQDELFAMTK RNVIPTMTQM NLKYAISAKN RARTVAGVSI LSTMTNRQYH QKMLKSMAAT RGATCVIGTT KFYGGWDFML KTLYKDVDNP HLMGWDYPKC DRAMPNMCRI FASLILARKH GTCCTTRDRF YRLANECAQV LSEYVLCGGG YYVKPGGTSS GDATTAYANS VFNILQATTA NVSALMGANG NKIVDKEVKD MQFDLYVNVY RSTSPDPKFV DKYYAFLNKH FSMMILSDDG VVCYNSDYAA KGYIAGIQNF KETLYYQNNV FMSEAKCWLE TDLKKGPHEF CSQHTLYIKD GDDGYFLPYP DPSRILSAGC FVDDIVKTDG TLMVERFVSL AIDAYPLTKH EDIEYQNVFW VYLQYIEKLY KDLTGHMLDS YSVMLCGDNS AKFWEEAFYR DLYSSPTTLQ AVGSCVVCHS QTSLRCGTCI RRPFLCCKCC YDHVIATPHK MVLSVSPYVC NAPGCGVSDV TKLYLGGMSY FCVDHRPVCS FPLCANGLVF GLYKNMCTGS PSIVEFNRLA TCDWTESGDY TLANTTTEPL KLFAAETLRA TEEASKQSYA IATIKEIVGE RQLLLVWEAG KSKPPLNRNY VFTGYHITKN SKVQLGEYIF ERIDYSDAVS YKSSTTYKLT VGDIFVLTSH SVATLMAPTI VNQERYVKIT GLYPTITVPE EFASHVANFQ KSGYSKYVTV QGPPGTGKSH FAIGLAIYYP TARVVYTACS HAAVDALCEK AFKYLNIAKC SRIIPAKARV ECYDRFKVNE TNSQYLFSTI NALPETSADI LVVDEVSMCT NYDLSIINAR IKAKHIVYVG DPAQLPAPRT LLTRGTLEPE NFNSVTRLMC NLGPDIFLSM CYRCPKEIVS TVSALVYNNK LLAKKEFSGQ CFKILYKGNV THDASSAINR PQLTFVKNFI TANPAWSKAV FISPYNSQNA VARSMLGLTT QTVDSSQGSE YQYVIFCQTA DTAHANNINR FNVAITRAQK GILCVMTSQA LFDSLEFTEL SFTNYKLQSQ IVTGLFKDCS RETSGLSPAY APTYVSVDDK YKTSDELCVN LNLPANVPYS RVISRMGFKL DATVPGYPKL FITREEAVRQ VRSWIGFDVE GAHASRNACG TNVPLQLGFS TGVNFVVQPV GVVDTEWGNM LTGIAARPPP GEQFKHLVPL MHKGAAWPIV RRRIVQMLSD TLDKLSDYCT FVCWAHGFEL TSASYFCKIG KEQKCCMCNR RAAAYSSPLQ SYACWTHSCG YDYVYNPFFV DVQQWGYVGN LATNHDRYCS VHQGAHVASN DAIMTRCLAI HSCFIERVDW DIEYPYISHE KKLNSCCRIV ERNVVRAALL AGSFDKVYDI GNPKGIPIFD DPVVDWHYFD AQPLTRKVQQ LFYTEDMASR FADGLCLFWN CNVPKYPNNA IVCRFDTRVH SEFNLPGCDG GSLYVNKHAF HTPAYDVSAF RDLKPLPFFY YSTTPCEVHG NGSMIEDIDY VPLKSAVCIT ACNLGGAVCR KHATEYREYM EAYNLVSASG FRLWCYKTFD IYNLWSTFTK VQGLENIAFN VVKQGHFIGV EGELPVAVVN DKIFTKSGVN DICMFENKTT LPTNIAFELY AKRAVRSHPD FKFLHNLQAD ICYKFVLWDY ERSNIYGTAT IGVCKYTDID VNSALNICFD IRDNGSLEKF MSTPNAIFIS DRKIKKYPCM VGPDYAYFNG AIIRDSDVVK QPVKFYLYKK VNNEFIDPTE CIYTQSRSCS DFLPLSDMEK DFLSFDSDVF IKKYGLENYA FEHVVYGDFS HTTLGGLHLL IGLYKKQQEG HIIMEEMLKG SSTIHNYFIT ETNTAAFKAV CSVIDLKLDD FVMILKSQDL GVVSKVVKVP IDLTMIEFML WCKDGQVQTF YPRLQASADW KPGHAMPSLF KVQNVNLERC ELANYKQSIP MPRGVHMNIA KYMQLCQYLN TCTLAVPANM RVIHFGAGSD KGIAPGTSVL RQWLPTDAII IDNDLNEFVS DADITLFGDC VTVRVGQQVD LVISDMYDPT TKNVTGSNES KALFFTYLCN LINNNLALGG SVAIKITEHS WSVELYELMG KFAWWTVFCT NANASSSEGF LLGINYLGTI KENIDGGAMH ANYIFWRNST PMNLSTYSLF DLSKFQLKLK GTSVLQLKES QINELVISLL SQGKLLIRDN DTLSVSTDVL VNTYRKLR //