ID A0A2N0L2A8_9CHLR Unreviewed; 253 AA. AC A0A2N0L2A8; DT 25-APR-2018, integrated into UniProtKB/TrEMBL. DT 25-APR-2018, sequence version 1. DT 31-JUL-2019, entry version 9. DE RecName: Full=tRNA pseudouridine synthase A {ECO:0000256|HAMAP-Rule:MF_00171}; DE EC=5.4.99.12 {ECO:0000256|HAMAP-Rule:MF_00171}; DE AltName: Full=tRNA pseudouridine(38-40) synthase {ECO:0000256|HAMAP-Rule:MF_00171}; DE AltName: Full=tRNA pseudouridylate synthase I {ECO:0000256|HAMAP-Rule:MF_00171}; DE AltName: Full=tRNA-uridine isomerase I {ECO:0000256|HAMAP-Rule:MF_00171}; GN Name=truA {ECO:0000256|HAMAP-Rule:MF_00171}; GN ORFNames=BZY82_11790 {ECO:0000313|EMBL:PKB64218.1}; OS SAR202 cluster bacterium Io17-Chloro-G3. OC Bacteria; Chloroflexi; SAR202 cluster. OX NCBI_TaxID=1926612 {ECO:0000313|EMBL:PKB64218.1, ECO:0000313|Proteomes:UP000232669}; RN [1] {ECO:0000313|EMBL:PKB64218.1, ECO:0000313|Proteomes:UP000232669} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=Io17-Chloro-G3 {ECO:0000313|EMBL:PKB64218.1}; RX PubMed=29208946; RA Mehrshad M., Rodriguez-Valera F., Amoozegar M.A., Lopez-Garcia P., RA Ghai R.; RT "The enigmatic SAR202 cluster up close: shedding light on a globally RT distributed dark ocean lineage involved in sulfur cycling."; RL ISME J. 0:0-0(2017). CC -!- FUNCTION: Formation of pseudouridine at positions 38, 39 and 40 in CC the anticodon stem and loop of transfer RNAs. {ECO:0000256|HAMAP- CC Rule:MF_00171}. CC -!- CATALYTIC ACTIVITY: CC Reaction=uridine(38/39/40) in tRNA = pseudouridine(38/39/40) in CC tRNA; Xref=Rhea:RHEA:22376, Rhea:RHEA-COMP:10085, Rhea:RHEA- CC COMP:10087, ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.12; CC Evidence={ECO:0000256|HAMAP-Rule:MF_00171, CC ECO:0000256|RuleBase:RU003792}; CC -!- SUBUNIT: Homodimer. {ECO:0000256|HAMAP-Rule:MF_00171}. CC -!- SIMILARITY: Belongs to the tRNA pseudouridine synthase TruA CC family. {ECO:0000256|HAMAP-Rule:MF_00171, CC ECO:0000256|RuleBase:RU003792, ECO:0000256|SAAS:SAAS00543779}. CC -!- CAUTION: Lacks conserved residue(s) required for the propagation CC of feature annotation. {ECO:0000256|HAMAP-Rule:MF_00171}. CC -!- CAUTION: The sequence shown here is derived from an CC EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is CC preliminary data. {ECO:0000313|EMBL:PKB64218.1}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; MUCQ01000100; PKB64218.1; -; Genomic_DNA. DR Proteomes; UP000232669; Unassembled WGS sequence. DR GO; GO:0003723; F:RNA binding; IEA:InterPro. DR GO; GO:0106029; F:tRNA pseudouridine synthase activity; IEA:UniProtKB-EC. DR GO; GO:0031119; P:tRNA pseudouridine synthesis; IEA:UniProtKB-UniRule. DR Gene3D; 3.30.70.660; -; 1. DR HAMAP; MF_00171; TruA; 1. DR InterPro; IPR020103; PsdUridine_synth_cat_dom_sf. DR InterPro; IPR001406; PsdUridine_synth_TruA. DR InterPro; IPR020097; PsdUridine_synth_TruA_a/b_dom. DR InterPro; IPR020095; PsdUridine_synth_TruA_C. DR PANTHER; PTHR11142; PTHR11142; 1. DR Pfam; PF01416; PseudoU_synth_1; 2. DR PIRSF; PIRSF001430; tRNA_psdUrid_synth; 1. DR SUPFAM; SSF55120; SSF55120; 1. DR TIGRFAMs; TIGR00071; hisT_truA; 1. PE 3: Inferred from homology; KW Complete proteome {ECO:0000313|Proteomes:UP000232669}; KW Isomerase {ECO:0000256|HAMAP-Rule:MF_00171, KW ECO:0000256|RuleBase:RU003792, ECO:0000256|SAAS:SAAS00119851}; KW tRNA processing {ECO:0000256|HAMAP-Rule:MF_00171, KW ECO:0000256|RuleBase:RU003792, ECO:0000256|SAAS:SAAS00427419}. FT DOMAIN 2 91 PseudoU_synth_1. {ECO:0000259|Pfam: FT PF01416}. FT DOMAIN 140 239 PseudoU_synth_1. {ECO:0000259|Pfam: FT PF01416}. FT ACT_SITE 47 47 Nucleophile. {ECO:0000256|HAMAP-Rule: FT MF_00171, ECO:0000256|PIRSR:PIRSR001430- FT 1}. FT BINDING 105 105 Substrate. {ECO:0000256|HAMAP-Rule: FT MF_00171, ECO:0000256|PIRSR:PIRSR001430- FT 2}. SQ SEQUENCE 253 AA; 27864 MW; BE5C4EBA4707FF4A CRC64; MVIEYDGTEY SGFQRQTKDP TVQSALEEAL TLLTGEQIHV NGASRTDSGA HALGQVVAFS TDSSLPLEAF VKGPNAHLPR EIAVLQAHLV PHSFDPRRDA TSRVYRYTIL NQATRSPSWE RWAYQVPGQL DLDAMSASLR SLEGSHDFSP FGGAVPSGRS TIRRIIRTQV IRYDNRVLVE IEGNAFLPGQ VRRTVGTLLK VGKGKLSPET VREIIDGSSD QRATTAVPAK GLCLMKIKYK DFPPNGYKTN ENL //