ID A0A2K7SP36_9VIBR Unreviewed; 306 AA. AC A0A2K7SP36; DT 28-MAR-2018, integrated into UniProtKB/TrEMBL. DT 28-MAR-2018, sequence version 1. DT 24-JAN-2024, entry version 27. DE RecName: Full=Glutaminase {ECO:0000256|ARBA:ARBA00012918, ECO:0000256|HAMAP-Rule:MF_00313}; DE EC=3.5.1.2 {ECO:0000256|ARBA:ARBA00012918, ECO:0000256|HAMAP-Rule:MF_00313}; GN Name=glsA {ECO:0000256|HAMAP-Rule:MF_00313, GN ECO:0000313|EMBL:BBL89972.1}; GN ORFNames=BI375_10855 {ECO:0000313|EMBL:OHY88880.1}, VroAM7_26250 GN {ECO:0000313|EMBL:BBL89972.1}; OS Vibrio rotiferianus. OC Bacteria; Pseudomonadota; Gammaproteobacteria; Vibrionales; Vibrionaceae; OC Vibrio. OX NCBI_TaxID=190895 {ECO:0000313|EMBL:OHY88880.1, ECO:0000313|Proteomes:UP000180133}; RN [1] {ECO:0000313|EMBL:OHY88880.1, ECO:0000313|Proteomes:UP000180133} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=HM-10 {ECO:0000313|EMBL:OHY88880.1, RC ECO:0000313|Proteomes:UP000180133}; RA Yang Q.; RT "Isolation, identification and antibiotic sensitivity analysis of bacterial RT pathogen from juvenile Hippocampus erectus with tail-rotted disease."; RL Submitted (SEP-2016) to the EMBL/GenBank/DDBJ databases. RN [2] {ECO:0000313|Proteomes:UP000315115} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=AM7 {ECO:0000313|Proteomes:UP000315115}; RA Miyazaki K., Wiseschart A., Pootanakit K., Ishimori K., Kitahara K.; RT "Complete Genome Sequences of Vibrion rotiferianus strain AM7."; RL Submitted (JUL-2019) to the EMBL/GenBank/DDBJ databases. RN [3] {ECO:0000313|EMBL:BBL89972.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=AM7 {ECO:0000313|EMBL:BBL89972.1}; RA Miyazaki K., Wiseschart A., Pootanakit K., Kitahara K.; RT "Complete Genome Sequence of Vibrio rotiferianus Strain AM7."; RL Microbiol. Resour. Announc. 9:e01591-19(2020). CC -!- CATALYTIC ACTIVITY: CC Reaction=H2O + L-glutamine = L-glutamate + NH4(+); CC Xref=Rhea:RHEA:15889, ChEBI:CHEBI:15377, ChEBI:CHEBI:28938, CC ChEBI:CHEBI:29985, ChEBI:CHEBI:58359; EC=3.5.1.2; CC Evidence={ECO:0000256|ARBA:ARBA00001062, ECO:0000256|HAMAP- CC Rule:MF_00313}; CC -!- SUBUNIT: Homotetramer. {ECO:0000256|ARBA:ARBA00011881, CC ECO:0000256|HAMAP-Rule:MF_00313}. CC -!- SIMILARITY: Belongs to the glutaminase family. CC {ECO:0000256|ARBA:ARBA00011076, ECO:0000256|HAMAP-Rule:MF_00313}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AP019798; BBL89972.1; -; Genomic_DNA. DR EMBL; MKFT01000060; OHY88880.1; -; Genomic_DNA. DR RefSeq; WP_005425799.1; NZ_VTXT01000036.1. DR AlphaFoldDB; A0A2K7SP36; -. DR SMR; A0A2K7SP36; -. DR GeneID; 83580569; -. DR OrthoDB; 9788822at2; -. DR Proteomes; UP000180133; Unassembled WGS sequence. DR Proteomes; UP000315115; Chromosome 1. DR GO; GO:0004359; F:glutaminase activity; IEA:UniProtKB-UniRule. DR GO; GO:0006541; P:glutamine metabolic process; IEA:InterPro. DR Gene3D; 3.40.710.10; DD-peptidase/beta-lactamase superfamily; 1. DR HAMAP; MF_00313; Glutaminase; 1. DR InterPro; IPR012338; Beta-lactam/transpept-like. DR InterPro; IPR015868; Glutaminase. DR NCBIfam; TIGR03814; Gln_ase; 1. DR PANTHER; PTHR12544; GLUTAMINASE; 1. DR PANTHER; PTHR12544:SF29; GLUTAMINASE; 1. DR Pfam; PF04960; Glutaminase; 1. DR SUPFAM; SSF56601; beta-lactamase/transpeptidase-like; 1. PE 3: Inferred from homology; KW Acetylation {ECO:0000256|HAMAP-Rule:MF_00313}; KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|HAMAP-Rule:MF_00313}. FT BINDING 64 FT /ligand="substrate" FT /evidence="ECO:0000256|HAMAP-Rule:MF_00313" FT BINDING 115 FT /ligand="substrate" FT /evidence="ECO:0000256|HAMAP-Rule:MF_00313" FT BINDING 159 FT /ligand="substrate" FT /evidence="ECO:0000256|HAMAP-Rule:MF_00313" FT BINDING 166 FT /ligand="substrate" FT /evidence="ECO:0000256|HAMAP-Rule:MF_00313" FT BINDING 190 FT /ligand="substrate" FT /evidence="ECO:0000256|HAMAP-Rule:MF_00313" FT BINDING 242 FT /ligand="substrate" FT /evidence="ECO:0000256|HAMAP-Rule:MF_00313" FT BINDING 260 FT /ligand="substrate" FT /evidence="ECO:0000256|HAMAP-Rule:MF_00313" SQ SEQUENCE 306 AA; 32846 MW; 5FFCB7CDFD05DE03 CRC64; MKPTAQILTD ILAEVRPLIG QGKVADYIPA LAKVPNTKLG IAVYTNEGEV IKAGDAEESF SIQSISKALS LTLAMCLYKQ EEIWCRVGKE PSGQAFNSMI QLEMEQGIPR NPFINAGAIV VADLLQSRLS APRQRLLEFA RQLSGDTHIV YDKVVAASEM MHGDRNAAIA YLMRSFGNFE NEVIPVLQNY FHACALKMSC VDLAKTFSYL ANKGTSVQTG KPVVSPTQTK QLNALLATCG LYDGAGEFAY RVGMPGKSGV GGGIIAVVPG EMTIAVWSPE LDASGNSLAG TKALELLAER IGRSIF //