ID A0A2K5DAB1_AOTNA Unreviewed; 397 AA. AC A0A2K5DAB1; DT 28-MAR-2018, integrated into UniProtKB/TrEMBL. DT 28-MAR-2018, sequence version 1. DT 27-NOV-2024, entry version 28. DE RecName: Full=Bis(monoacylglycero)phosphate synthase CLN5 {ECO:0000256|ARBA:ARBA00044532}; DE EC=3.1.2.22 {ECO:0000256|ARBA:ARBA00012423}; DE AltName: Full=Ceroid-lipofuscinosis neuronal protein 5 {ECO:0000256|ARBA:ARBA00044547}; DE AltName: Full=Palmitoyl protein thioesterase CLN5 {ECO:0000256|ARBA:ARBA00044557}; DE AltName: Full=S-depalmitoylase CLN5 {ECO:0000256|ARBA:ARBA00044556}; GN Name=CLN5 {ECO:0000313|Ensembl:ENSANAP00000017898.1}; OS Aotus nancymaae (Ma's night monkey). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Platyrrhini; Aotidae; OC Aotus. OX NCBI_TaxID=37293 {ECO:0000313|Ensembl:ENSANAP00000017898.1, ECO:0000313|Proteomes:UP000233020}; RN [1] {ECO:0000313|Ensembl:ENSANAP00000017898.1} RP IDENTIFICATION. RG Ensembl; RL Submitted (SEP-2023) to UniProtKB. CC -!- FUNCTION: Catalyzes the synthesis of bis(monoacylglycero)phosphate CC (BMP) via transacylation of 2 molecules of lysophosphatidylglycerol CC (LPG). BMP also known as lysobisphosphatidic acid plays a key role in CC the formation of intraluminal vesicles and in maintaining intracellular CC cholesterol homeostasis. Can use only LPG as the exclusive CC lysophospholipid acyl donor for base exchange and displays BMP synthase CC activity towards various LPGs (LPG 14:0, LPG 16:0, LPG 18:0, LPG 18:1) CC with a higher preference for longer chain lengths. Plays a role in CC influencing the retrograde trafficking of lysosomal sorting receptors CC SORT1 and IGF2R from the endosomes to the trans-Golgi network by CC controlling the recruitment of retromer complex to the endosomal CC membrane. Regulates the localization and activation of RAB7A which is CC required to recruit the retromer complex to the endosomal membrane. CC {ECO:0000256|ARBA:ARBA00045492}. CC -!- FUNCTION: Exhibits palmitoyl protein thioesterase (S-depalmitoylation) CC activity in vitro and most likely plays a role in protein S- CC depalmitoylation. {ECO:0000256|ARBA:ARBA00044494}. CC -!- CATALYTIC ACTIVITY: CC Reaction=2 3-(9Z-octadecenoyl)-sn-glycero-1-phospho-(1'-sn-glycerol) = CC 3-(9Z-octadecenoyl)-sn-glycero-1-phospho-(3'-(9Z-octadecenoyl)-1'-sn- CC glycerol) + sn-glycero-1-phospho-(1'-sn-glycerol); CC Xref=Rhea:RHEA:77599, ChEBI:CHEBI:139150, ChEBI:CHEBI:139152, CC ChEBI:CHEBI:197411; Evidence={ECO:0000256|ARBA:ARBA00044481}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:77600; CC Evidence={ECO:0000256|ARBA:ARBA00044481}; CC -!- CATALYTIC ACTIVITY: CC Reaction=2 3-hexadecanoyl-sn-glycero-1-phospho-(1'-sn-glycerol) = 3- CC hexadecanoyl-sn-glycero-1-phospho-(3'-hexadecanoyl-1'-sn-glycerol) + CC sn-glycero-1-phospho-(1'-sn-glycerol); Xref=Rhea:RHEA:77607, CC ChEBI:CHEBI:44859, ChEBI:CHEBI:197411, ChEBI:CHEBI:197415; CC Evidence={ECO:0000256|ARBA:ARBA00044455}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:77608; CC Evidence={ECO:0000256|ARBA:ARBA00044455}; CC -!- CATALYTIC ACTIVITY: CC Reaction=2 3-octadecanoyl-sn-glycero-1-phospho-(1'-sn-glycerol) = 3- CC octadecanoyl-sn-glycero-1-phospho-(3'-octadecanoyl-1'-sn-glycerol) + CC sn-glycero-1-phospho-(1'-sn-glycerol); Xref=Rhea:RHEA:77603, CC ChEBI:CHEBI:197411, ChEBI:CHEBI:197412, ChEBI:CHEBI:197414; CC Evidence={ECO:0000256|ARBA:ARBA00044460}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:77604; CC Evidence={ECO:0000256|ARBA:ARBA00044460}; CC -!- CATALYTIC ACTIVITY: CC Reaction=2 3-tetradecanoyl-sn-glycero-1-phospho-(1'-sn-glycerol) = 3- CC tetradecanoyl-sn-glycero-1-phospho-(3'-tetradecanoyl-1'-sn-glycerol) CC + sn-glycero-1-phospho-(1'-sn-glycerol); Xref=Rhea:RHEA:77611, CC ChEBI:CHEBI:197411, ChEBI:CHEBI:197413, ChEBI:CHEBI:197416; CC Evidence={ECO:0000256|ARBA:ARBA00044453}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:77612; CC Evidence={ECO:0000256|ARBA:ARBA00044453}; CC -!- CATALYTIC ACTIVITY: CC Reaction=2 a 3-acyl-sn-glycero-1-phospho-(1'-sn-glycerol) = a 3-acyl- CC sn-glycero-1-phospho-(3'-acyl-1'-sn-glycerol) + sn-glycero-1-phospho- CC (1'-sn-glycerol); Xref=Rhea:RHEA:77619, ChEBI:CHEBI:77717, CC ChEBI:CHEBI:197411, ChEBI:CHEBI:197425; CC Evidence={ECO:0000256|ARBA:ARBA00044472}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:77620; CC Evidence={ECO:0000256|ARBA:ARBA00044472}; CC -!- CATALYTIC ACTIVITY: CC Reaction=S-hexadecanoyl-L-cysteinyl-[protein] + H2O = L-cysteinyl- CC [protein] + hexadecanoate + H(+); Xref=Rhea:RHEA:19233, Rhea:RHEA- CC COMP:10131, Rhea:RHEA-COMP:11032, ChEBI:CHEBI:7896, CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:29950, CC ChEBI:CHEBI:74151; EC=3.1.2.22; CC Evidence={ECO:0000256|ARBA:ARBA00037021}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:19234; CC Evidence={ECO:0000256|ARBA:ARBA00037021}; CC -!- SIMILARITY: Belongs to the CLN5 family. CC {ECO:0000256|ARBA:ARBA00007028}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR AlphaFoldDB; A0A2K5DAB1; -. DR STRING; 37293.ENSANAP00000017898; -. DR Ensembl; ENSANAT00000035759.1; ENSANAP00000017898.1; ENSANAG00000026805.1. DR GeneTree; ENSGT00390000010065; -. DR OMA; FRPHQSF; -. DR Proteomes; UP000233020; Unplaced. DR GO; GO:0005829; C:cytosol; IEA:GOC. DR GO; GO:0005783; C:endoplasmic reticulum; IEA:Ensembl. DR GO; GO:0005794; C:Golgi apparatus; IEA:Ensembl. DR GO; GO:0005765; C:lysosomal membrane; IEA:Ensembl. DR GO; GO:0048471; C:perinuclear region of cytoplasm; IEA:Ensembl. DR GO; GO:0005775; C:vacuolar lumen; IEA:Ensembl. DR GO; GO:0160121; F:bis(monoacylglycero)phosphate synthase activity; IEA:Ensembl. DR GO; GO:0005537; F:D-mannose binding; IEA:Ensembl. DR GO; GO:0016798; F:hydrolase activity, acting on glycosyl bonds; IEA:TreeGrafter. DR GO; GO:0052816; F:long-chain fatty acyl-CoA hydrolase activity; IEA:Ensembl. DR GO; GO:0007420; P:brain development; IEA:Ensembl. DR GO; GO:0070085; P:glycosylation; IEA:Ensembl. DR GO; GO:0007042; P:lysosomal lumen acidification; IEA:Ensembl. DR GO; GO:0022008; P:neurogenesis; IEA:Ensembl. DR GO; GO:0042147; P:retrograde transport, endosome to Golgi; IEA:Ensembl. DR GO; GO:0006465; P:signal peptide processing; IEA:Ensembl. DR GO; GO:0007601; P:visual perception; IEA:Ensembl. DR InterPro; IPR026138; CLN5. DR PANTHER; PTHR15380:SF2; CEROID-LIPOFUSCINOSIS NEURONAL PROTEIN 5; 1. DR PANTHER; PTHR15380; CEROID-LIPOFUSCINOSIS, NEURONAL 5; 1. DR Pfam; PF15014; CLN5; 1. PE 3: Inferred from homology; KW Glycoprotein {ECO:0000256|ARBA:ARBA00023180}; KW Reference proteome {ECO:0000313|Proteomes:UP000233020}. FT REGION 1..37 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" SQ SEQUENCE 397 AA; 44974 MW; BDC1B865B3929ECF CRC64; GHGGLRPGLA APRKLRPPAS RGSGGAGSTG GSLMAPEGDT ALGVVMRRSA GGARGRASRR WALALLCLAA APGWLGASGS PSRRHWPEPY KRFDFRPKPD PYCQAKYTFC PTGSPIPVMK DDDDIEVFRL QTPVWEFKYG DLLGHLKIMH DAIGFRSTLT GKNYTVEWYE LFQLGNCTFP HLRPEMDAPF WCNQGAACFF EGIDDAHWKE NGTLVQVATI SGNMFNKMAK WVKQDNETGI YYETWTVKAS PEKGAETWFD SYDCSKFVLR TYNKLAEFGA EFKKIETNYT RIFLYSGEPT YLGNETSVFG PTGNKTLALA IKKFYYPFKP HLSTKEFLLS LLQIFDAVIV HRQFYLFYNF EYWFLPMKFP FIKITYEEIP LPNRNNTLSA RRGSSHL //