ID A0A2K2A5G6_POPTR Unreviewed; 411 AA. AC A0A2K2A5G6; DT 28-MAR-2018, integrated into UniProtKB/TrEMBL. DT 28-MAR-2018, sequence version 1. DT 29-SEP-2021, entry version 15. DE RecName: Full=CN hydrolase domain-containing protein {ECO:0000259|PROSITE:PS50263}; GN ORFNames=POPTR_006G207700 {ECO:0000313|EMBL:PNT32777.1}; OS Populus trichocarpa (Western balsam poplar) (Populus balsamifera subsp. OS trichocarpa). OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Malpighiales; Salicaceae; Saliceae; Populus. OX NCBI_TaxID=3694 {ECO:0000313|EMBL:PNT32777.1, ECO:0000313|Proteomes:UP000006729}; RN [1] {ECO:0000313|EMBL:PNT32777.1, ECO:0000313|Proteomes:UP000006729} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=cv. Nisqually {ECO:0000313|Proteomes:UP000006729}; RX PubMed=16973872; DOI=10.1126/science.1128691; RA Tuskan G.A., Difazio S., Jansson S., Bohlmann J., Grigoriev I., RA Hellsten U., Putnam N., Ralph S., Rombauts S., Salamov A., Schein J., RA Sterck L., Aerts A., Bhalerao R.R., Bhalerao R.P., Blaudez D., Boerjan W., RA Brun A., Brunner A., Busov V., Campbell M., Carlson J., Chalot M., RA Chapman J., Chen G.L., Cooper D., Coutinho P.M., Couturier J., Covert S., RA Cronk Q., Cunningham R., Davis J., Degroeve S., Dejardin A., RA Depamphilis C., Detter J., Dirks B., Dubchak I., Duplessis S., Ehlting J., RA Ellis B., Gendler K., Goodstein D., Gribskov M., Grimwood J., Groover A., RA Gunter L., Hamberger B., Heinze B., Helariutta Y., Henrissat B., RA Holligan D., Holt R., Huang W., Islam-Faridi N., Jones S., RA Jones-Rhoades M., Jorgensen R., Joshi C., Kangasjarvi J., Karlsson J., RA Kelleher C., Kirkpatrick R., Kirst M., Kohler A., Kalluri U., Larimer F., RA Leebens-Mack J., Leple J.C., Locascio P., Lou Y., Lucas S., Martin F., RA Montanini B., Napoli C., Nelson D.R., Nelson C., Nieminen K., Nilsson O., RA Pereda V., Peter G., Philippe R., Pilate G., Poliakov A., Razumovskaya J., RA Richardson P., Rinaldi C., Ritland K., Rouze P., Ryaboy D., Schmutz J., RA Schrader J., Segerman B., Shin H., Siddiqui A., Sterky F., Terry A., RA Tsai C.J., Uberbacher E., Unneberg P., Vahala J., Wall K., Wessler S., RA Yang G., Yin T., Douglas C., Marra M., Sandberg G., Van de Peer Y., RA Rokhsar D.; RT "The genome of black cottonwood, Populus trichocarpa (Torr. & Gray)."; RL Science 313:1596-1604(2006). CC -!- CATALYTIC ACTIVITY: CC Reaction=3-cyano-L-alanine + 2 H2O = L-aspartate + NH4(+); CC Xref=Rhea:RHEA:11188, ChEBI:CHEBI:15377, ChEBI:CHEBI:28938, CC ChEBI:CHEBI:29991, ChEBI:CHEBI:77860; EC=3.5.5.4; CC Evidence={ECO:0000256|ARBA:ARBA00000322}; CC -!- SIMILARITY: Belongs to the carbon-nitrogen hydrolase superfamily. CC Nitrilase family. {ECO:0000256|ARBA:ARBA00008129}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; CM009295; PNT32777.1; -; Genomic_DNA. DR EnsemblPlants; PNT32777; PNT32777; POPTR_006G207700v3. DR Gramene; PNT32777; PNT32777; POPTR_006G207700v3. DR OMA; ARDNQIY; -. DR Proteomes; UP000006729; Chromosome 6. DR GO; GO:0047427; F:cyanoalanine nitrilase activity; IEA:UniProtKB-EC. DR GO; GO:0000257; F:nitrilase activity; IBA:GO_Central. DR GO; GO:0018822; F:nitrile hydratase activity; IBA:GO_Central. DR GO; GO:0051410; P:detoxification of nitrogen compound; IBA:GO_Central. DR GO; GO:0006807; P:nitrogen compound metabolic process; IEA:InterPro. DR CDD; cd07564; nitrilases_CHs; 1. DR Gene3D; 3.60.110.10; -; 1. DR InterPro; IPR003010; C-N_Hydrolase. DR InterPro; IPR036526; C-N_Hydrolase_sf. DR InterPro; IPR000132; Nitrilase/CN_hydratase_CS. DR InterPro; IPR044149; Nitrilases_CHs. DR PANTHER; PTHR46044; PTHR46044; 1. DR Pfam; PF00795; CN_hydrolase; 1. DR SUPFAM; SSF56317; SSF56317; 1. DR PROSITE; PS50263; CN_HYDROLASE; 1. DR PROSITE; PS00920; NITRIL_CHT_1; 1. PE 3: Inferred from homology; KW Reference proteome {ECO:0000313|Proteomes:UP000006729}. FT DOMAIN 128..393 FT /note="CN hydrolase" FT /evidence="ECO:0000259|PROSITE:PS50263" FT REGION 71..101 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT ACT_SITE 168 FT /note="Proton acceptor" FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10139" SQ SEQUENCE 411 AA; 44005 MW; D0929313A459C2B3 CRC64; MEESTKNAEA KKEKKRGHRP LIVAQSPAEK AHIMVPAMCS TAIYRLRGEL GLGSCGAAIH WLVHHARPDL IPAPEPPTKT KSAKTCPSRK TDSVDHDPIP KPACMARADG DTPVSDFPAT ARAARSPVRA TVVQASTVVF DTPATLDKAE RLIAGAAAYG SQLVVFPEAF VGGYPRSVRF DATNPTEGDD GLQRYYASAI DVPGPEVERL AKIAGKYKVH LVMGVVERAG FYLYSTMLFF DSQGQHLGQH RKITLVASES AVWNSGGKST LPIYETSIGK IGGLTCWDNK WPLLRTELYD KGVEIYCAPT ADAGEIWKAS MIHIALEGGC FVLSANQFCR RRDYPFPPGD SNGDASLDAI TCAGGSVIIS PSGTILAGPS YHGECLISAD LDLGDIILAK TKYGGIRTMS G //