ID A0A2J6LZ50_LACSA Unreviewed; 209 AA. AC A0A2J6LZ50; DT 28-MAR-2018, integrated into UniProtKB/TrEMBL. DT 28-MAR-2018, sequence version 1. DT 10-APR-2019, entry version 7. DE RecName: Full=Thymidine kinase {ECO:0000256|RuleBase:RU000544}; DE EC=2.7.1.21 {ECO:0000256|RuleBase:RU000544}; GN ORFNames=LSAT_7X96100 {ECO:0000313|EMBL:PLY92607.1}; OS Lactuca sativa (Garden lettuce). OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliophyta; eudicotyledons; Gunneridae; OC Pentapetalae; asterids; campanulids; Asterales; Asteraceae; OC Cichorioideae; Cichorieae; Lactucinae; Lactuca. OX NCBI_TaxID=4236 {ECO:0000313|EMBL:PLY92607.1, ECO:0000313|Proteomes:UP000235145}; RN [1] {ECO:0000313|EMBL:PLY92607.1, ECO:0000313|Proteomes:UP000235145} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=cv. Salinas {ECO:0000313|Proteomes:UP000235145}; RX PubMed=28401891; DOI=10.1038/ncomms14953; RA Reyes-Chin-Wo S., Wang Z., Yang X., Kozik A., Arikit S., Song C., RA Xia L., Froenicke L., Lavelle D.O., Truco M.J., Xia R., Zhu S., Xu C., RA Xu H., Xu X., Cox K., Korf I., Meyers B.C., Michelmore R.W.; RT "Genome assembly with in vitro proximity ligation data and whole- RT genome triplication in lettuce."; RL Nat. Commun. 8:14953-14953(2017). CC -!- CATALYTIC ACTIVITY: CC Reaction=ATP + thymidine = ADP + dTMP + H(+); CC Xref=Rhea:RHEA:19129, ChEBI:CHEBI:15378, ChEBI:CHEBI:17748, CC ChEBI:CHEBI:30616, ChEBI:CHEBI:63528, ChEBI:CHEBI:456216; CC EC=2.7.1.21; Evidence={ECO:0000256|RuleBase:RU000544}; CC -!- SIMILARITY: Belongs to the thymidine kinase family. CC {ECO:0000256|RuleBase:RU004165}. CC -!- CAUTION: The sequence shown here is derived from an CC EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is CC preliminary data. {ECO:0000313|EMBL:PLY92607.1}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; NBSK01001899; PLY92607.1; -; Genomic_DNA. DR UniGene; Lsa.24549; -. DR Proteomes; UP000235145; Unassembled WGS sequence. DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW. DR GO; GO:0004797; F:thymidine kinase activity; IEA:UniProtKB-EC. DR GO; GO:0071897; P:DNA biosynthetic process; IEA:UniProtKB-KW. DR InterPro; IPR027417; P-loop_NTPase. DR InterPro; IPR001267; Thymidine_kinase. DR InterPro; IPR020633; Thymidine_kinase_CS. DR PANTHER; PTHR11441; PTHR11441; 1. DR Pfam; PF00265; TK; 1. DR PIRSF; PIRSF035805; TK_cell; 1. DR SUPFAM; SSF52540; SSF52540; 1. DR PROSITE; PS00603; TK_CELLULAR_TYPE; 1. PE 3: Inferred from homology; KW ATP-binding {ECO:0000256|RuleBase:RU000544}; KW Complete proteome {ECO:0000313|Proteomes:UP000235145}; KW DNA synthesis {ECO:0000256|RuleBase:RU000544}; KW Kinase {ECO:0000256|RuleBase:RU000544}; KW Nucleotide-binding {ECO:0000256|RuleBase:RU000544}; KW Reference proteome {ECO:0000313|Proteomes:UP000235145}; KW Transferase {ECO:0000256|RuleBase:RU000544}. FT ACT_SITE 108 108 Proton acceptor. {ECO:0000256|PIRSR: FT PIRSR035805-1}. SQ SEQUENCE 209 AA; 23310 MW; 2752C928965B5FD6 CRC64; MSLTPTVNHS DRDLMAQPLR PSGEIHVIVG PMFAGKTTAL LRRIKSEGTN GRNIAMIKSS KDTRYAVNSV VTHDGTKYPC WALPDLLSFK QKFGEEAYEK LDVIGIDEAQ FFDDLYEFCC KAADTDGKTV IVAGLDGDYL RRNFGPVLDI VPLADTITKL TARCEVCGKK AHFTFRKTKE TKTELIGGAD MYMPVCRQHY VNGQMVIKH //