ID A0A2I6SFE6_9POXV Unreviewed; 1164 AA. AC A0A2I6SFE6; DT 28-MAR-2018, integrated into UniProtKB/TrEMBL. DT 28-MAR-2018, sequence version 1. DT 03-AUG-2022, entry version 16. DE RecName: Full=DNA-directed RNA polymerase {ECO:0000256|ARBA:ARBA00012418}; DE EC=2.7.7.6 {ECO:0000256|ARBA:ARBA00012418}; GN Name=EVH146 {ECO:0000313|EMBL:AUO16289.1}; GN Synonyms=EVI146 {ECO:0000313|EMBL:CAG7614957.1}, EVM1146 GN {ECO:0000313|EMBL:CAG7621142.1}, EVM4146 GN {ECO:0000313|EMBL:CAG7621312.1}, EVM5146 GN {ECO:0000313|EMBL:CAG7621247.1}, EVMK146 GN {ECO:0000313|EMBL:CAG7621205.1}; OS Ectromelia virus. OC Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes; OC Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus. OX NCBI_TaxID=12643 {ECO:0000313|EMBL:AUO16289.1}; RN [1] {ECO:0000313|EMBL:AUO16289.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=Hampstead {ECO:0000313|EMBL:AUO16289.1}; RA Mavian C., Lopez-Bueno A., Alcami A.; RT "Comparative pathogenesis and genome sequence of ectromelia virus isolates RT from laboratory mouse outbreaks, or a wild mouse, and attenuated strains."; RL Submitted (JAN-2017) to the EMBL/GenBank/DDBJ databases. RN [2] {ECO:0000313|EMBL:CAG7621205.1} RP NUCLEOTIDE SEQUENCE. RA Mavian C., Lopez-Bueno A., Martin R., Nitsche A., Alcami A.; RL Submitted (JUN-2021) to the EMBL/GenBank/DDBJ databases. RN [3] {ECO:0000313|EMBL:CAG7614957.1} RP NUCLEOTIDE SEQUENCE. RA Mavian C., Lopez-Bueno A., Martin R., Alcami A.; RL Submitted (JUN-2021) to the EMBL/GenBank/DDBJ databases. CC -!- CATALYTIC ACTIVITY: CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA- CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395; CC EC=2.7.7.6; Evidence={ECO:0000256|ARBA:ARBA00024550}; CC -!- SUBCELLULAR LOCATION: Virion {ECO:0000256|ARBA:ARBA00004328}. CC -!- SIMILARITY: Belongs to the RNA polymerase beta chain family. CC {ECO:0000256|ARBA:ARBA00006835, ECO:0000256|RuleBase:RU000434}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; KY554976; AUO16289.1; -; Genomic_DNA. DR EMBL; OU343113; CAG7614957.1; -; Genomic_DNA. DR EMBL; OU343158; CAG7621142.1; -; Genomic_DNA. DR EMBL; OU343159; CAG7621205.1; -; Genomic_DNA. DR EMBL; OU343155; CAG7621247.1; -; Genomic_DNA. DR EMBL; OU343157; CAG7621312.1; -; Genomic_DNA. DR RefSeq; NP_671646.1; NC_004105.1. DR SMR; A0A2I6SFE6; -. DR GeneID; 951543; -. DR KEGG; vg:951543; -. DR Proteomes; UP000694220; Chromosome: 1. DR Proteomes; UP000694222; Chromosome: 1. DR Proteomes; UP000694226; Chromosome: 1. DR Proteomes; UP000694230; Chromosome: 1. DR Proteomes; UP000694245; Chromosome: 1. DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW. DR GO; GO:0003677; F:DNA binding; IEA:InterPro. DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-EC. DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW. DR GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro. DR GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro. DR Gene3D; 2.40.270.10; -; 1. DR Gene3D; 2.40.50.150; -; 1. DR InterPro; IPR015712; DNA-dir_RNA_pol_su2. DR InterPro; IPR007120; DNA-dir_RNAP_su2_dom. DR InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf. DR InterPro; IPR024390; RNA_pol_132_poxvirus. DR InterPro; IPR007121; RNA_pol_bsu_CS. DR InterPro; IPR007645; RNA_pol_Rpb2_3. DR InterPro; IPR007647; RNA_pol_Rpb2_5. DR InterPro; IPR007641; RNA_pol_Rpb2_7. DR InterPro; IPR014724; RNA_pol_RPB2_OB-fold. DR PANTHER; PTHR20856; PTHR20856; 1. DR Pfam; PF04565; RNA_pol_Rpb2_3; 1. DR Pfam; PF04567; RNA_pol_Rpb2_5; 1. DR Pfam; PF00562; RNA_pol_Rpb2_6; 1. DR Pfam; PF04560; RNA_pol_Rpb2_7; 1. DR Pfam; PF12415; rpo132; 1. DR PROSITE; PS01166; RNA_POL_BETA; 1. PE 3: Inferred from homology; KW DNA-directed RNA polymerase {ECO:0000256|ARBA:ARBA00022478}; KW Metal-binding {ECO:0000256|ARBA:ARBA00022723}; KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695}; KW Transcription {ECO:0000256|ARBA:ARBA00023163}; KW Transferase {ECO:0000256|ARBA:ARBA00022679}; KW Virion {ECO:0000256|ARBA:ARBA00022844}. FT DOMAIN 429..494 FT /note="RNA_pol_Rpb2_3" FT /evidence="ECO:0000259|Pfam:PF04565" FT DOMAIN 616..656 FT /note="RNA_pol_Rpb2_5" FT /evidence="ECO:0000259|Pfam:PF04567" FT DOMAIN 679..1047 FT /note="RNA_pol_Rpb2_6" FT /evidence="ECO:0000259|Pfam:PF00562" FT DOMAIN 1049..1141 FT /note="RNA_pol_Rpb2_7" FT /evidence="ECO:0000259|Pfam:PF04560" SQ SEQUENCE 1164 AA; 133429 MW; 81563DB6CB6DA3B6 CRC64; MKKNTDSEMD QRLGYKFLVP DPKAGVFYRP LHFQYVSYSN FILHRLHEIL TVKRPLLSFK NNTERIMIEI SNVKVTPPDY SPIIASIKGK SYDALATFTV NIFKEVMTKE GISITKISSY EGKDSHLIKI PLLIGYGNKN PLDTAKYLVP NVIGGVFINK QSVEKVGINL VEKITTWPKF RVVKPNSFTF SFSSVSPPNV LPTRYRHYKI SLDISQLEAS NISSTKTFIT VNIVLLSQYL SRVSLEFIRR SLSYDMPPEV VYLVNAIIDS AKRITESITD FNIDTYINDL VEAEHIKQKS QLTINEFKYE MLHNFLPHMN YTPDQLKGFY MISLLRKFLY CIYHTSRYPD RDSMVCHRIL TYGKYFETLA HDELENYIGN IRNDIMNNHK NRGTYAVNIH VLTTPGLNHA FSSLLSGKFK KSDGSYRTHP HYSWMQNISI PRSVGFYPDQ VKISKMFSVR KYHPSQYLYF CSSDVPERGP QVGLVSQLSV LSSITNILTS EYLDLEKKIC EYIRSYYKDD ISYFETGFPI TIENALVASL NPNMICDFVT DFRRRKRMGF FGNLEVGITL VRDHMNEIRI NIGAGRLVRP FLVVDNGELM MDVCQELESR LDDMTFSDIQ KEFPHVIEMV DIEQFTFSNV CESVQKFRMM SKDERKQYDL CDFPAEFRDG YVASSLVGIN HNSGPRAILG CAQAKQAISC LSSDIRNKID NGIHLMYPER PIVISKALET SKIAANCFGQ HVTIALMSYK GINQEDGIII KKQFIQRGGL DIVTAKKHQV EIPLENFNNK ERDRSNAYSK LESNGLVRLN AFLESGDAMA RNISSRTLED DFARDNQISF DVSEKYTDMY KSRVERVQVE LTDKVKVRVL TMKERRPILG DKFTTRTSQK GTVAYIADET ELPYDENGIT PDVIINSTSI FSRKTISMLI EVILTAAYSA KPYNNNGENR PVCFPSSNET SIDTYMQFAK QCYEHSNPQL TDDELSDKVF CEKILYDPET DKPYASKVFF GPIYYLRLRH LTQDKATVRC RGKKTKLIRQ ANEGRKRGGG IKFGEMERDC LIAHGAANTI TEVLKDSEED YQDVYICENC GDIAAQIKGI NTCLRCSKLN LSPLLTKIDT THVSKVFLTQ MNARGVKVKL DFERRPPSFY KQLDKVDLKP SFLK //