ID A0A2H6J849_9BACT Unreviewed; 152 AA. AC A0A2H6J849; DT 28-FEB-2018, integrated into UniProtKB/TrEMBL. DT 28-FEB-2018, sequence version 1. DT 02-JUN-2021, entry version 16. DE RecName: Full=Deoxyuridine 5'-triphosphate nucleotidohydrolase {ECO:0000256|HAMAP-Rule:MF_00116}; DE Short=dUTPase {ECO:0000256|HAMAP-Rule:MF_00116}; DE EC=3.6.1.23 {ECO:0000256|HAMAP-Rule:MF_00116}; DE AltName: Full=dUTP pyrophosphatase {ECO:0000256|HAMAP-Rule:MF_00116}; GN Name=dut {ECO:0000256|HAMAP-Rule:MF_00116, GN ECO:0000313|EMBL:GBE46922.1}; GN ORFNames=BMS3Bbin12_00075 {ECO:0000313|EMBL:GBE46922.1}; OS bacterium BMS3Bbin12. OC Bacteria. OX NCBI_TaxID=2005735 {ECO:0000313|EMBL:GBE46922.1, ECO:0000313|Proteomes:UP000236623}; RN [1] {ECO:0000313|Proteomes:UP000236623} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RA Kato S., Shibuya T., Takaki Y., Hirai M., Nunoura T., Suzuki K.; RT "Genome-enabled metabolic reconstruction of dominant chemosynthetic RT colonizers in deep-sea massive sulfide deposits."; RL Environ. Microbiol. 0:0-0(2017). CC -!- FUNCTION: This enzyme is involved in nucleotide metabolism: it produces CC dUMP, the immediate precursor of thymidine nucleotides and it decreases CC the intracellular concentration of dUTP so that uracil cannot be CC incorporated into DNA. {ECO:0000256|HAMAP-Rule:MF_00116}. CC -!- CATALYTIC ACTIVITY: CC Reaction=dUTP + H2O = diphosphate + dUMP + H(+); Xref=Rhea:RHEA:10248, CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:33019, CC ChEBI:CHEBI:61555, ChEBI:CHEBI:246422; EC=3.6.1.23; CC Evidence={ECO:0000256|ARBA:ARBA00000878, ECO:0000256|HAMAP- CC Rule:MF_00116}; CC -!- COFACTOR: CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420; CC Evidence={ECO:0000256|HAMAP-Rule:MF_00116}; CC -!- PATHWAY: Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP CC route): step 2/2. {ECO:0000256|HAMAP-Rule:MF_00116}. CC -!- SIMILARITY: Belongs to the dUTPase family. {ECO:0000256|HAMAP- CC Rule:MF_00116}. CC -!- CAUTION: Lacks conserved residue(s) required for the propagation of CC feature annotation. {ECO:0000256|HAMAP-Rule:MF_00116}. CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ CC whole genome shotgun (WGS) entry which is preliminary data. CC {ECO:0000313|EMBL:GBE46922.1}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; BDTV01000006; GBE46922.1; -; Genomic_DNA. DR EnsemblBacteria; GBE46922; GBE46922; BMS3Bbin12_00075. DR UniPathway; UPA00610; UER00666. DR Proteomes; UP000236623; Unassembled WGS sequence. DR GO; GO:0004170; F:dUTP diphosphatase activity; IEA:UniProtKB-UniRule. DR GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule. DR GO; GO:0006226; P:dUMP biosynthetic process; IEA:UniProtKB-UniPathway. DR GO; GO:0046081; P:dUTP catabolic process; IEA:InterPro. DR CDD; cd07557; trimeric_dUTPase; 1. DR Gene3D; 2.70.40.10; -; 1. DR HAMAP; MF_00116; dUTPase_bact; 1. DR InterPro; IPR029054; dUTPase-like. DR InterPro; IPR036157; dUTPase-like_sf. DR InterPro; IPR008181; dUTPase_1. DR InterPro; IPR033704; dUTPase_trimeric. DR PANTHER; PTHR11241; PTHR11241; 1. DR Pfam; PF00692; dUTPase; 1. DR SUPFAM; SSF51283; SSF51283; 1. DR TIGRFAMs; TIGR00576; dut; 1. PE 3: Inferred from homology; KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|HAMAP-Rule:MF_00116}; KW Magnesium {ECO:0000256|HAMAP-Rule:MF_00116}; KW Metal-binding {ECO:0000256|HAMAP-Rule:MF_00116}; KW Nucleotide metabolism {ECO:0000256|ARBA:ARBA00023080, ECO:0000256|HAMAP- KW Rule:MF_00116}. FT DOMAIN 19..150 FT /note="dUTPase" FT /evidence="ECO:0000259|Pfam:PF00692" FT REGION 71..73 FT /note="Substrate binding" FT /evidence="ECO:0000256|HAMAP-Rule:MF_00116" FT REGION 88..90 FT /note="Substrate binding" FT /evidence="ECO:0000256|HAMAP-Rule:MF_00116" FT BINDING 84 FT /note="Substrate" FT /evidence="ECO:0000256|HAMAP-Rule:MF_00116" SQ SEQUENCE 152 AA; 16066 MW; F1106FC66EB3A9CF CRC64; MPRTVQLKVL DPRIGREIPP PDYATAGAAG LDLRACLDAP ITLAAGETTL IPTGIAIHID DPGLAAVLLP RSGLGHKHGI VLGNLVGLID SDYQGQVFVS CWNRGGAPFT IEIGERIAQM VFMPVVQARF ERVEEFEASA RGAGGFGHSG RL //