ID   A0A2E8MY86_9RICK        Unreviewed;       382 AA.
AC   A0A2E8MY86;
DT   31-JAN-2018, integrated into UniProtKB/TrEMBL.
DT   31-JAN-2018, sequence version 1.
DT   14-DEC-2022, entry version 14.
DE   RecName: Full=isoleucine--tRNA ligase {ECO:0000256|ARBA:ARBA00013165};
DE            EC=6.1.1.5 {ECO:0000256|ARBA:ARBA00013165};
DE   Flags: Fragment;
GN   ORFNames=CMP48_08980 {ECO:0000313|EMBL:MBR07809.1};
OS   Rickettsiales bacterium.
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rickettsiales.
OX   NCBI_TaxID=2026788 {ECO:0000313|EMBL:MBR07809.1, ECO:0000313|Proteomes:UP000230568};
RN   [1] {ECO:0000313|Proteomes:UP000230568}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Tully B.J., Graham E.D., Heidelberg J.F.;
RT   "The Reconstruction of 2,631 Draft Metagenome-Assembled Genomes from the
RT   Global Oceans.";
RL   Submitted (SEP-2017) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS
CC       can inadvertently accommodate and process structurally similar amino
CC       acids such as valine, to avoid such errors it has two additional
CC       distinct tRNA(Ile)-dependent editing activities. One activity is
CC       designated as 'pretransfer' editing and involves the hydrolysis of
CC       activated Val-AMP. The other activity is designated 'posttransfer'
CC       editing and involves deacylation of mischarged Val-tRNA(Ile).
CC       {ECO:0000256|ARBA:ARBA00025217}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-
CC         isoleucyl-tRNA(Ile); Xref=Rhea:RHEA:11060, Rhea:RHEA-COMP:9666,
CC         Rhea:RHEA-COMP:9695, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019,
CC         ChEBI:CHEBI:58045, ChEBI:CHEBI:78442, ChEBI:CHEBI:78528,
CC         ChEBI:CHEBI:456215; EC=6.1.1.5;
CC         Evidence={ECO:0000256|ARBA:ARBA00000114};
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:MBR07809.1}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; PBTY01000026; MBR07809.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A2E8MY86; -.
DR   Proteomes; UP000230568; Unassembled WGS sequence.
DR   GO; GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004822; F:isoleucine-tRNA ligase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006428; P:isoleucyl-tRNA aminoacylation; IEA:InterPro.
DR   Gene3D; 3.40.50.620; -; 1.
DR   Gene3D; 3.90.740.10; -; 1.
DR   InterPro; IPR002300; aa-tRNA-synth_Ia.
DR   InterPro; IPR002301; Ile-tRNA-ligase.
DR   InterPro; IPR023586; Ile-tRNA-ligase_type2.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR   PANTHER; PTHR42780; SOLEUCYL-TRNA SYNTHETASE; 1.
DR   Pfam; PF00133; tRNA-synt_1; 1.
DR   PRINTS; PR00984; TRNASYNTHILE.
DR   SUPFAM; SSF50677; ValRS/IleRS/LeuRS editing domain; 1.
PE   4: Predicted;
KW   Aminoacyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00023146};
KW   ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW   Ligase {ECO:0000256|ARBA:ARBA00022598, ECO:0000313|EMBL:MBR07809.1};
KW   Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW   Protein biosynthesis {ECO:0000256|ARBA:ARBA00022917}.
FT   DOMAIN          19..380
FT                   /note="tRNA-synt_1"
FT                   /evidence="ECO:0000259|Pfam:PF00133"
FT   NON_TER         382
FT                   /evidence="ECO:0000313|EMBL:MBR07809.1"
SQ   SEQUENCE   382 AA;  43464 MW;  60A813E8E54BB2C4 CRC64;
     MAKYPEYKQV SYPQIAEEVL KFWADQQIFE KSVTEREGAE TFTFYEGPPS ANGTPGIHHV
     MARTVKDIFC RYKTQKGYQV KRKGGWDTHG LPVELQVEKE LGIKKDDIGK TISVAEYNQK
     CKETVMRFKD EWDRLTQQMG YWVDLDDPYI TFDQKYMESL WHLLKKLYEK GLLYKGYTVQ
     PYSPAAGTGL SSHELNQPGT YKDVKDTSIV AQFKVKKDDR SSFLFESDDE DVRILAWTTT
     PWTLPSNCAL AVGEKITYVK VKSYNQYTGT AISVVLAKDL VGKFFSDKAK EVAFGDYKLG
     DKLIPFEIVS EFTGKDILEV RYEQLMPYVT NEDLEKNAFR VIPGDFVSTE DGTGVVHTAS
     VFGADDFRVA QQAGVPAVMV KD
//