ID A0A2D9VNQ2_9RHOB Unreviewed; 262 AA. AC A0A2D9VNQ2; DT 31-JAN-2018, integrated into UniProtKB/TrEMBL. DT 31-JAN-2018, sequence version 1. DT 19-JAN-2022, entry version 17. DE RecName: Full=Enoyl-[acyl-carrier-protein] reductase [NADH] {ECO:0000256|PIRNR:PIRNR000094}; DE EC=1.3.1.9 {ECO:0000256|PIRNR:PIRNR000094}; GN ORFNames=CL812_13545 {ECO:0000313|EMBL:MAQ46855.1}; OS Actibacterium sp. OC Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; OC Roseobacteraceae; Actibacterium; unclassified Actibacterium. OX NCBI_TaxID=1872125 {ECO:0000313|EMBL:MAQ46855.1, ECO:0000313|Proteomes:UP000229580}; RN [1] {ECO:0000313|Proteomes:UP000229580} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RA Tully B.J., Graham E.D., Heidelberg J.F.; RT "The Reconstruction of 2,631 Draft Metagenome-Assembled Genomes from the RT Global Oceans."; RL Submitted (SEP-2017) to the EMBL/GenBank/DDBJ databases. CC -!- CATALYTIC ACTIVITY: CC Reaction=a 2,3-saturated acyl-[ACP] + NAD(+) = a (2E)-enoyl-[ACP] + CC H(+) + NADH; Xref=Rhea:RHEA:10240, Rhea:RHEA-COMP:9925, Rhea:RHEA- CC COMP:9926, ChEBI:CHEBI:15378, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, CC ChEBI:CHEBI:78784, ChEBI:CHEBI:78785; EC=1.3.1.9; CC Evidence={ECO:0000256|ARBA:ARBA00000175, CC ECO:0000256|PIRNR:PIRNR000094}; CC -!- PATHWAY: Lipid metabolism; fatty acid biosynthesis. CC {ECO:0000256|ARBA:ARBA00005194}. CC -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR) CC family. FabI subfamily. {ECO:0000256|ARBA:ARBA00009233, CC ECO:0000256|PIRNR:PIRNR000094}. CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ CC whole genome shotgun (WGS) entry which is preliminary data. CC {ECO:0000313|EMBL:MAQ46855.1}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; NZUI01000022; MAQ46855.1; -; Genomic_DNA. DR EnsemblBacteria; MAQ46855; MAQ46855; CL812_13545. DR UniPathway; UPA00094; -. DR Proteomes; UP000229580; Unassembled WGS sequence. DR GO; GO:0004318; F:enoyl-[acyl-carrier-protein] reductase (NADH) activity; IEA:UniProtKB-EC. DR GO; GO:0016631; F:enoyl-[acyl-carrier-protein] reductase activity; IEA:UniProtKB-EC. DR GO; GO:0016616; F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor; IEA:UniProt. DR GO; GO:0006633; P:fatty acid biosynthetic process; IEA:UniProtKB-UniPathway. DR CDD; cd05372; ENR_SDR; 1. DR InterPro; IPR014358; Enoyl-ACP_Rdtase_NADH. DR InterPro; IPR036291; NAD(P)-bd_dom_sf. DR InterPro; IPR002347; SDR_fam. DR PANTHER; PTHR43159; PTHR43159; 1. DR PIRSF; PIRSF000094; Enoyl-ACP_rdct; 1. DR PRINTS; PR00081; GDHRDH. DR SUPFAM; SSF51735; SSF51735; 1. PE 3: Inferred from homology; KW Fatty acid biosynthesis {ECO:0000256|ARBA:ARBA00023160, KW ECO:0000256|PIRNR:PIRNR000094}; KW Fatty acid metabolism {ECO:0000256|ARBA:ARBA00023160, KW ECO:0000256|PIRNR:PIRNR000094}; KW Lipid biosynthesis {ECO:0000256|PIRNR:PIRNR000094}; KW Lipid metabolism {ECO:0000256|ARBA:ARBA00023160, KW ECO:0000256|PIRNR:PIRNR000094}; KW NAD {ECO:0000256|ARBA:ARBA00023027, ECO:0000256|PIRNR:PIRNR000094}; KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002, KW ECO:0000256|PIRNR:PIRNR000094}. FT NP_BIND 20..21 FT /note="NAD" FT /evidence="ECO:0000256|PIRSR:PIRSR000094-3" FT NP_BIND 65..66 FT /note="NAD" FT /evidence="ECO:0000256|PIRSR:PIRSR000094-3" FT ACT_SITE 146 FT /note="Proton acceptor" FT /evidence="ECO:0000256|PIRSR:PIRSR000094-1" FT ACT_SITE 156 FT /note="Proton acceptor" FT /evidence="ECO:0000256|PIRSR:PIRSR000094-1" FT BINDING 14 FT /note="NAD; via carbonyl oxygen" FT /evidence="ECO:0000256|PIRSR:PIRSR000094-3" FT BINDING 41 FT /note="NAD" FT /evidence="ECO:0000256|PIRSR:PIRSR000094-3" FT BINDING 93 FT /note="NAD; via carbonyl oxygen" FT /evidence="ECO:0000256|PIRSR:PIRSR000094-3" FT BINDING 163 FT /note="NAD" FT /evidence="ECO:0000256|PIRSR:PIRSR000094-3" SQ SEQUENCE 262 AA; 27877 MW; D05268FF4D594003 CRC64; MAELMKGKRG LIMGVANERS IAWGIAKALA AEGAELAFSY QGESFGKRVA PLAESVGSDF LVDVDVTDDA SLDAAFGEIE KRWGKLDFVV HAIAFSDKNE LTGRFINTSR ENFKTSLVIS CYSLIDVARR ARPLMTDGGS IITLTYQGSN KVTPFYNVMG VAKSALESAM RYLANDLGPE GIRVNAISPG PMKTLAGAAI GGARKTFRMT EANAPLRHNA TLEAIGGTAV YLASDYGNCT TGEIVTVDGG FHILGMPQPD NL //