ID A0A249N4T7_LACRH Unreviewed; 320 AA. AC A0A249N4T7; DT 20-DEC-2017, integrated into UniProtKB/TrEMBL. DT 20-DEC-2017, sequence version 1. DT 14-DEC-2022, entry version 14. DE RecName: Full=N(4)-(Beta-N-acetylglucosaminyl)-L-asparaginase {ECO:0000256|Google:UnProtein}; GN ORFNames=N507_1498 {ECO:0000313|EMBL:ASY48673.1}; OS Lacticaseibacillus rhamnosus DSM 14870. OC Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae; OC Lacticaseibacillus. OX NCBI_TaxID=1380361 {ECO:0000313|EMBL:ASY48673.1, ECO:0000313|Proteomes:UP000217181}; RN [1] {ECO:0000313|EMBL:ASY48673.1, ECO:0000313|Proteomes:UP000217181} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=DSM 14870 {ECO:0000313|EMBL:ASY48673.1, RC ECO:0000313|Proteomes:UP000217181}; RA Marcotte H., Brandsborg E., Hammarstrom L.; RT "EcoVag, a novel probiotic vaginal preparation restoring the vaginal RT microbiota in bacterial vaginosis."; RL Submitted (OCT-2013) to the EMBL/GenBank/DDBJ databases. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; CP006804; ASY48673.1; -; Genomic_DNA. DR RefSeq; WP_005687925.1; NZ_CP006804.1. DR AlphaFoldDB; A0A249N4T7; -. DR EnsemblBacteria; ASY48673; ASY48673; N507_1498. DR Proteomes; UP000217181; Chromosome. DR GO; GO:0016787; F:hydrolase activity; IEA:InterPro. DR InterPro; IPR029055; Ntn_hydrolases_N. DR InterPro; IPR000246; Peptidase_T2. DR PANTHER; PTHR10188; L-ASPARAGINASE; 1. DR Pfam; PF01112; Asparaginase_2; 1. DR SUPFAM; SSF56235; N-terminal nucleophile aminohydrolases (Ntn hydrolases); 1. PE 4: Predicted; FT ACT_SITE 154 FT /note="Nucleophile" FT /evidence="ECO:0000256|PIRSR:PIRSR600246-1" FT BINDING 182..185 FT /ligand="substrate" FT /evidence="ECO:0000256|PIRSR:PIRSR600246-2" FT BINDING 205..208 FT /ligand="substrate" FT /evidence="ECO:0000256|PIRSR:PIRSR600246-2" FT SITE 153..154 FT /note="Cleavage; by autolysis" FT /evidence="ECO:0000256|PIRSR:PIRSR600246-3" SQ SEQUENCE 320 AA; 34375 MW; D236DFFB3A5E98ED CRC64; MNETAWGVIA TWRMAHDGVK KASQLLQANG TAGDAVEALI KTVEAYPYYK SVGYGGLPNE NGEVEMDAAF MDGDTLAQGA VAGIHEVLHA VSVARALSHD HYNSFLVGQG ATQYAQLNGF EMRNMLTDRA KKRWEKRRAE LADAKIKPYD GHDTVGAITL APTGSMAAAT STSGLFMKRP GRVGDSPLSG SGFYVDSDIG GAAATGLGED IMKGCLSYEI VRRMGEGRTP QEACDEAVYP FIEKLKRRYG KAGEFSLIAL DRAGNWGVAT NVEFTFSVAT ASQKPVILMA NPGPNQTTTI TPVTQAWLDA YEKRIKAPIK //